RU2008150910A - METHOD FOR PREPARING SAMPLES OF BIOLOGICAL TISSUES FOR RESEARCH IN A SCANNING ELECTRONIC MICROSCOPE - Google Patents
METHOD FOR PREPARING SAMPLES OF BIOLOGICAL TISSUES FOR RESEARCH IN A SCANNING ELECTRONIC MICROSCOPE Download PDFInfo
- Publication number
- RU2008150910A RU2008150910A RU2008150910/12A RU2008150910A RU2008150910A RU 2008150910 A RU2008150910 A RU 2008150910A RU 2008150910/12 A RU2008150910/12 A RU 2008150910/12A RU 2008150910 A RU2008150910 A RU 2008150910A RU 2008150910 A RU2008150910 A RU 2008150910A
- Authority
- RU
- Russia
- Prior art keywords
- biological tissues
- camphene
- research
- preparing samples
- electronic microscope
- Prior art date
Links
Landscapes
- Sampling And Sample Adjustment (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Способ подготовки образцов биологических тканей для исследования в сканирующем электронном микроскопе, включающий фиксацию, промывку и обезвоживание, отличающийся тем, что после обезвоживания, не высушивая, образцы переносят в смесь дегидранта и камфена, затем последовательно пропитывают в двух порциях расплавленного камфена при t° 51-60°С, после пропитывания камфеном образец высушивают на воздухе. A method of preparing samples of biological tissues for examination in a scanning electron microscope, including fixation, washing and dehydration, characterized in that after dehydration, without drying, the samples are transferred to a mixture of dehydrant and camphene, then they are subsequently impregnated in two portions of molten camphene at t ° 51- 60 ° C, after soaking with camphene, the sample is dried in air.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2008150910/12A RU2397472C1 (en) | 2008-12-22 | 2008-12-22 | Method of preparing biological tissue samples for analysis in scanning electron microscope |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2008150910/12A RU2397472C1 (en) | 2008-12-22 | 2008-12-22 | Method of preparing biological tissue samples for analysis in scanning electron microscope |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2008150910A true RU2008150910A (en) | 2010-06-27 |
| RU2397472C1 RU2397472C1 (en) | 2010-08-20 |
Family
ID=42683214
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2008150910/12A RU2397472C1 (en) | 2008-12-22 | 2008-12-22 | Method of preparing biological tissue samples for analysis in scanning electron microscope |
Country Status (1)
| Country | Link |
|---|---|
| RU (1) | RU2397472C1 (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102680289B (en) * | 2011-03-08 | 2014-09-24 | 国家纳米科学中心 | Method for preparing scanning electron microscope samples from biological samples |
| RU2466375C1 (en) * | 2011-04-07 | 2012-11-10 | Федеральное государственное бюджетное учреждение "Российский научный центр "Восстановительная травматология и ортопедия" имени академика Г.А. Илизарова" Министерства здравоохранения и социального развития Российской Федерации | Method for preparing samples of non-decalcified articular cartilage with subjacent subchondral bone for multipurpose study |
| RU2484446C1 (en) * | 2011-12-28 | 2013-06-10 | Федеральное государственное бюджетное учреждение "Российский научный центр "Восстановительная травматология и ортопедия" имени академика Г.А. Илизарова" Министерства здравоохранения и социального развития Российской Федерации | Method to prepare samples of microorganisms biofilms for survey in scanning electronic microscope |
| RU2564895C1 (en) * | 2014-06-09 | 2015-10-10 | Федеральное государственное бюджетное научное учреждение "Научно-исследовательский институт комплексных проблем сердечно-сосудистых заболеваний" (НИИ КПССЗ) | Method for making biological tissue samples with implanted elements for light microscopy study |
| RU2668879C1 (en) * | 2017-11-07 | 2018-10-04 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Уральский государственный медицинский университет" Министерства здравоохранения Российской Федерации (ФГБОУ ВО УГМУ Минздрава России) | Method for preparing the surface of bone tissue samples to study their microstructure using a scanning electron microscope |
| RU2747640C1 (en) * | 2020-04-23 | 2021-05-11 | Общество С Ограниченной Ответственностью "Международный Центр Медицинских Исследований И Разработок" (Ооо "Мц Миир") | Method for preparing large samples of regenerating bone for scanning electron microscopy |
-
2008
- 2008-12-22 RU RU2008150910/12A patent/RU2397472C1/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| RU2397472C1 (en) | 2010-08-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| RU2008150910A (en) | METHOD FOR PREPARING SAMPLES OF BIOLOGICAL TISSUES FOR RESEARCH IN A SCANNING ELECTRONIC MICROSCOPE | |
| Rosario et al. | Decellularization and sterilization of porcine urinary bladder matrix for tissue engineering in the lower urinary tract | |
| Williams et al. | Partially digested adult cardiac extracellular matrix promotes cardiomyocyte proliferation in vitro | |
| WO2018187521A3 (en) | Methods of detecting cell-free dna in biological samples | |
| CN104888273B (en) | A kind of double-layer composite endocranium and preparation method thereof | |
| Yuan et al. | Dyeing behaviors of ionic liquid treated wool | |
| RU2011143787A (en) | METHOD FOR DETECTING SUBSTANCE IN BIOLOGICAL SAMPLE | |
| EP2610402A3 (en) | Clothes dryer and control method thereof | |
| DE602006017878D1 (en) | IDENTIFICATION OF FÖTAL CELLS FROM MATTERIAL BLOOD | |
| WO2015123192A3 (en) | Universal system, method and solution for the acceleration of the process of fixing, dehydrating and clearing the structure of biological tissue | |
| RU2484446C1 (en) | Method to prepare samples of microorganisms biofilms for survey in scanning electronic microscope | |
| RU2011109741A (en) | METHOD FOR DETERMINING ELASTICITY OF BLOOD CELLS | |
| Buravkov et al. | Simple method of specimen preparation for scanning electron microscopy. | |
| RU2013141705A (en) | METHOD OF MICROSCOPIC STUDY OF NATIVE LANGUAGE ROOT SCRAPING | |
| CN102835387A (en) | Method for preparing sealed glass slide for microscope | |
| CN206848018U (en) | A kind of pedotheque air-dry apparatus | |
| FI20115157A0 (en) | A method for improving the quality and functionality of filter paper suitable for biological sample collection | |
| CN107261206B (en) | Bionic bone tissue engineering scaffold and preparation method and application thereof | |
| RU2009137971A (en) | METHOD OF OPTICAL ENLIGHTENMENT OF SAMPLES OF BIOLOGICAL TISSUES | |
| CN205513413U (en) | Neonate's heel blood blood sampling piece storage rack | |
| Kajbafzadeh et al. | Optimization and critical evaluation of decellularization and recellularization strategies to develop bioengineered heart: comparison of two different protocols | |
| Meng et al. | Comparison of adsorption behaviour for fibrinogen and albumin on single walled carbon nanotubes nonwoven | |
| CN104402967B (en) | One kind rapid extraction method of protein from simple grain cottonseed cotton-wool | |
| Xiaoqing et al. | Influences of silk fibroin on the compressive strength and injectability of calcium phosphate cement | |
| UA88914C2 (en) | method for preparation of bile micro-preparation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM4A | The patent is invalid due to non-payment of fees |
Effective date: 20101223 |