RU2008138616A - METHOD FOR PRODUCING PURIN RIBONUCLEOSIDES AND RIBONUCLEOTIDES - Google Patents

METHOD FOR PRODUCING PURIN RIBONUCLEOSIDES AND RIBONUCLEOTIDES Download PDF

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RU2008138616A
RU2008138616A RU2008138616/13A RU2008138616A RU2008138616A RU 2008138616 A RU2008138616 A RU 2008138616A RU 2008138616/13 A RU2008138616/13 A RU 2008138616/13A RU 2008138616 A RU2008138616 A RU 2008138616A RU 2008138616 A RU2008138616 A RU 2008138616A
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bacterium
purine
purine nucleoside
producing
nutrient medium
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RU2008138616/13A
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Russian (ru)
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RU2422510C2 (en
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Сергей Викторович Гронский (RU)
Сергей Викторович Гронский
Дмитрий Владимирович Романенков (RU)
Дмитрий Владимирович Романенков
Наталия Павловна Закатаева (RU)
Наталия Павловна Закатаева
Такаюки АСАХАРА (JP)
Такаюки АСАХАРА
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Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU)
Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ)
Аджиномото Ко., Инк. (JP)
Аджиномото Ко., Инк.
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Priority to RU2008138616/10A priority Critical patent/RU2422510C2/en
Priority to CN200980138368.1A priority patent/CN102171346B/en
Priority to JP2011513790A priority patent/JP5488594B2/en
Priority to PCT/JP2009/067447 priority patent/WO2010038903A1/en
Publication of RU2008138616A publication Critical patent/RU2008138616A/en
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Publication of RU2422510C2 publication Critical patent/RU2422510C2/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/32—Nucleotides having a condensed ring system containing a six-membered ring having two N-atoms in the same ring, e.g. purine nucleotides, nicotineamide-adenine dinucleotide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

1. Бактерия, принадлежащая к роду Bacillus, способная к продукции пуриновых нуклеозидов, отличающаяся тем, что указанная бактерия модифицирована таким образом, что экспрессия гена, кодирующего 3-гексулозо-6-фосфатсинтазу(HPS), в указанной бактерии ослаблена. ! 2. Бактерия по п.1, отличающаяся тем, что указанная экспрессия ослаблена за счет инактивации указанного гена. ! 3. Бактерия по п.1, отличающаяся тем, что указанным геном является ген hxlA. ! 4. Бактерия по п.1, отличающаяся тем, что указанной бактерией является бактерия В.subtilis. ! 5. Бактерия по п.1, отличающаяся тем, что указанной бактерией является бактерия В.amyloliquefaciens. ! 6. Бактерия по любому из пп.1-5, отличающаяся тем, что указанный пуриновый нуклеозид выбран из группы, состоящей из инозина, ксантозина, гуанозина и аденозина. ! 7. Способ получения пуринового нуклеозида, включающий культивирование бактерии по любому из пп.1-6 в питательной среде, приводящее к секреции указанного пуринового нуклеозида в указанную питательную среду, и выделение указанного пуринового нуклеотида из культуральной жидкости. ! 8. Способ получения пуринового нуклеозида по п.7, отличающийся тем, что указанный пуриновый нуклеозид выбран из группы, состоящей из инозина, ксантозина, гуанозина и аденозина. ! 9. Способ получения пуринового нуклеотида, включающий культивирование бактерии по любому из пп.1-6 в питательной среде, приводящее к секреции указанного пуринового нуклеозида в указанную питательную среду, выделение указанного пуринового нуклеозида из культуральной жидкости, фосфорилирование указанного пуринового нуклеозида и выделение пуринового нуклеотида. ! 10. Способ получения пуринового нуклео 1. A bacterium belonging to the genus Bacillus, capable of producing purine nucleosides, characterized in that the bacterium is modified in such a way that the expression of the gene encoding 3-hexulose-6-phosphate synthase (HPS) in this bacterium is weakened. ! 2. The bacterium according to claim 1, characterized in that said expression is attenuated due to inactivation of said gene. ! 3. The bacterium according to claim 1, characterized in that said gene is an hxlA gene. ! 4. The bacterium according to claim 1, characterized in that said bacterium is the bacterium B. subtilis. ! 5. The bacterium according to claim 1, characterized in that said bacterium is the bacterium B. amyloliquefaciens. ! 6. The bacterium according to any one of claims 1 to 5, characterized in that said purine nucleoside is selected from the group consisting of inosine, xanthosine, guanosine and adenosine. ! 7. A method of producing a purine nucleoside, comprising cultivating the bacterium according to any one of claims 1 to 6 in a nutrient medium, leading to the secretion of said purine nucleoside into said nutrient medium, and isolation of said purine nucleotide from the culture fluid. ! 8. The method for producing purine nucleoside according to claim 7, characterized in that said purine nucleoside is selected from the group consisting of inosine, xanthosine, guanosine and adenosine. ! 9. A method for producing a purine nucleotide, comprising culturing the bacterium according to any one of claims 1 to 6 in a nutrient medium, leading to the secretion of said purine nucleoside into the specified nutrient medium, isolation of said purine nucleoside from the culture fluid, phosphorylation of said purine nucleoside and isolation of purine nucleotide. ! 10. The method of obtaining purine nucleo

Claims (10)

1. Бактерия, принадлежащая к роду Bacillus, способная к продукции пуриновых нуклеозидов, отличающаяся тем, что указанная бактерия модифицирована таким образом, что экспрессия гена, кодирующего 3-гексулозо-6-фосфатсинтазу(HPS), в указанной бактерии ослаблена.1. A bacterium belonging to the genus Bacillus, capable of producing purine nucleosides, characterized in that the bacterium is modified in such a way that the expression of the gene encoding 3-hexulose-6-phosphate synthase (HPS) in this bacterium is weakened. 2. Бактерия по п.1, отличающаяся тем, что указанная экспрессия ослаблена за счет инактивации указанного гена.2. The bacterium according to claim 1, characterized in that said expression is attenuated due to inactivation of said gene. 3. Бактерия по п.1, отличающаяся тем, что указанным геном является ген hxlA.3. The bacterium according to claim 1, characterized in that said gene is an hxlA gene. 4. Бактерия по п.1, отличающаяся тем, что указанной бактерией является бактерия В.subtilis.4. The bacterium according to claim 1, characterized in that said bacterium is the bacterium B. subtilis. 5. Бактерия по п.1, отличающаяся тем, что указанной бактерией является бактерия В.amyloliquefaciens.5. The bacterium according to claim 1, characterized in that said bacterium is the bacterium B. amyloliquefaciens. 6. Бактерия по любому из пп.1-5, отличающаяся тем, что указанный пуриновый нуклеозид выбран из группы, состоящей из инозина, ксантозина, гуанозина и аденозина.6. The bacterium according to any one of claims 1 to 5, characterized in that said purine nucleoside is selected from the group consisting of inosine, xanthosine, guanosine and adenosine. 7. Способ получения пуринового нуклеозида, включающий культивирование бактерии по любому из пп.1-6 в питательной среде, приводящее к секреции указанного пуринового нуклеозида в указанную питательную среду, и выделение указанного пуринового нуклеотида из культуральной жидкости.7. A method of producing a purine nucleoside, comprising cultivating the bacterium according to any one of claims 1 to 6 in a nutrient medium, leading to the secretion of said purine nucleoside into said nutrient medium, and isolation of said purine nucleotide from the culture fluid. 8. Способ получения пуринового нуклеозида по п.7, отличающийся тем, что указанный пуриновый нуклеозид выбран из группы, состоящей из инозина, ксантозина, гуанозина и аденозина.8. The method for producing purine nucleoside according to claim 7, characterized in that said purine nucleoside is selected from the group consisting of inosine, xanthosine, guanosine and adenosine. 9. Способ получения пуринового нуклеотида, включающий культивирование бактерии по любому из пп.1-6 в питательной среде, приводящее к секреции указанного пуринового нуклеозида в указанную питательную среду, выделение указанного пуринового нуклеозида из культуральной жидкости, фосфорилирование указанного пуринового нуклеозида и выделение пуринового нуклеотида.9. A method for producing a purine nucleotide, comprising culturing the bacterium according to any one of claims 1 to 6 in a nutrient medium, leading to the secretion of said purine nucleoside into the specified nutrient medium, isolation of said purine nucleoside from the culture fluid, phosphorylation of said purine nucleoside and isolation of purine nucleotide. 10. Способ получения пуринового нуклеотида по п.9, отличающийся тем, что пуриновый нуклеотид выбран из группы, состоящей из 5'-инозиновой кислоты, ксантозин-5'-фосфата, 5'-гуаниловой кислоты и 5'-адениловой кислоты. 10. The method for producing purine nucleotide according to claim 9, characterized in that the purine nucleotide is selected from the group consisting of 5'-inosinic acid, xanthosine-5'-phosphate, 5'-guanilic acid and 5'-adenylic acid.
RU2008138616/10A 2008-09-30 2008-09-30 Method of producing purine ribonucleoside and ribonucleotides RU2422510C2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
RU2008138616/10A RU2422510C2 (en) 2008-09-30 2008-09-30 Method of producing purine ribonucleoside and ribonucleotides
CN200980138368.1A CN102171346B (en) 2008-09-30 2009-09-30 A method for producing purine ribonucleosides and ribonucleotides
JP2011513790A JP5488594B2 (en) 2008-09-30 2009-09-30 Method for producing purine ribonucleoside and ribonucleotide
PCT/JP2009/067447 WO2010038903A1 (en) 2008-09-30 2009-09-30 A method for producing purine ribonucleosides and ribonucleotides

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CN103150487B (en) * 2012-11-29 2016-05-11 扬州大学 A kind of relevant key gene network regulation method of chicken muscle inosinicacid route of synthesis that builds
WO2014153207A2 (en) * 2013-03-14 2014-09-25 The Regents Of The University Of California Recombinant microorganisms having a methanol elongation cycle (mec)
US9518278B2 (en) 2013-03-14 2016-12-13 The Regents Of The University Of California Recombinant microorganisms having a methanol elongation cycle (MEC)
CN103146786A (en) * 2013-03-25 2013-06-12 天津科技大学 Method for producing adenosine by sequentially controlling fermentation with gradient pH
RU2542387C1 (en) * 2013-09-27 2015-02-20 Федеральное государственное унитарное предприятие "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов" (ФГУП "ГосНИИгенетика") BACTERIA Bacillus subtilis PRODUCING 5`-AMINOIMIDAZOLE-4-CARBOXAMIDERIBOSIDE (AICAR), AND METHOD OF MICROBIOLOGICAL SYNTHESIS OF AICAR BY CULTURING THIS BACTERIUM
CN105670979B (en) * 2016-04-26 2019-03-26 南京工业大学 Nucleoside phosphorylase, coding gene, high-yield strain thereof and application
EP3861109A1 (en) 2018-10-05 2021-08-11 Ajinomoto Co., Inc. Method for producing target substance by bacterial fermentation
CN112574934B (en) * 2020-10-12 2022-05-06 廊坊梅花生物技术开发有限公司 Engineering bacterium for high yield of guanosine as well as construction method and application thereof
CN112592881B (en) * 2021-02-25 2021-06-11 中国科学院天津工业生物技术研究所 Engineering bacillus subtilis for high-efficiency exogenous protein expression and high-density culture

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JP2002017351A (en) * 2000-07-07 2002-01-22 Ajinomoto Co Inc Phosphohexuloisomerase and its gene
US7326546B2 (en) * 2005-03-10 2008-02-05 Ajinomoto Co., Inc. Purine-derived substance-producing bacterium and a method for producing purine-derived substance
RU2365622C2 (en) * 2006-12-22 2009-08-27 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) METHOD OF PURINE NUCLEOZIDES AND NUCLEOTIDES PRODUCTION BY FERMENTATION WITH APPLICATION OF BACTERIA BELONGING TO GENUS Escherichia OR Bacillus

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CN102171346A (en) 2011-08-31
CN102171346B (en) 2014-09-03
WO2010038903A1 (en) 2010-04-08
RU2422510C2 (en) 2011-06-27
JP2012503973A (en) 2012-02-16
JP5488594B2 (en) 2014-05-14

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