RU2006104582A - METHOD FOR DETERMINING VITAMIN B6 IN BIOLOGICALLY ACTIVE ADDITIVES AND SENSOR FOR ITS IMPLEMENTATION - Google Patents
METHOD FOR DETERMINING VITAMIN B6 IN BIOLOGICALLY ACTIVE ADDITIVES AND SENSOR FOR ITS IMPLEMENTATION Download PDFInfo
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- RU2006104582A RU2006104582A RU2006104582/28A RU2006104582A RU2006104582A RU 2006104582 A RU2006104582 A RU 2006104582A RU 2006104582/28 A RU2006104582/28 A RU 2006104582/28A RU 2006104582 A RU2006104582 A RU 2006104582A RU 2006104582 A RU2006104582 A RU 2006104582A
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Abstract
1. Сенсор для количественного определения витамина В6 в биологически активных добавках, состоящий из индикаторного химически модифицированного электрода, хлорид-серебрянных электродов сравнения и вспомогательного электрода, отличающийся повышением экспрессивности и селективности определения витамина В6.2. Способ количественного определения витамина В6 с помощью химического модифицированного электрода отличающийся простотой подготовки индикаторного электрода, постоянством его метрологических характеристик, позволяющих получать воспроизводимые аналитические сигналы от витамина В6, процесс съемки вольтамперограм происходящий с использованием трех электродной ячейки, состоящей из индикаторного химически модифицированного электрода, насыщенных хлорид-серебряных электродов сравнения и вспомогательного электрода в присутствии фонового электролита фосфатного буфера 0,025 М, ph=6,86, регистрация анодных пиков проводится в дифференциально импульсном режиме съемки вольтамперограмм при линейной скорости развертки потенциала 50 мВ/с, концентрация витамина определяется по относительному изменению тока электроокисления (высоте пика) витамина в интервале потенциалов от +0,4 - +1,4 В в диапазоне потенциалов +0,8- +0,9 В методом добавок аттестованных смесей, важным при определении витамина В6 является использование дифференциально импульсного режима съемки вольтамперограмм, существенным преимуществом такого вида съемки служит возможность получения высоких пиков, облегчающих расшифровку вольтамперограмм и уменьшающих ошибку измерения величины сигнала.1. A sensor for the quantitative determination of vitamin B6 in biologically active additives, consisting of a chemically modified indicator electrode, silver-silver reference electrodes and an auxiliary electrode, characterized by an increase in the expressivity and selectivity of determining vitamin B6.2. A method for quantitative determination of vitamin B6 using a chemical modified electrode is characterized by the simplicity of preparation of the indicator electrode, the constancy of its metrological characteristics, which allow to obtain reproducible analytical signals from vitamin B6, the process of shooting voltammograms using three electrode cells, consisting of an indicator chemically modified electrode, saturated with chloride silver reference electrodes and an auxiliary electrode in the presence of backgrounds phosphate buffer electrolyte 0.025 M, ph = 6.86, anode peaks are recorded in a differential pulse mode voltammograms at a linear potential scan rate of 50 mV / s, the vitamin concentration is determined by the relative change in the electrooxidation current (peak height) of the vitamin in the range of potentials from +0.4 - +1.4 V in the potential range + 0.8- +0.9 V using the method of additives of certified mixtures, it is important in determining vitamin B6 to use a differential-pulse mode for shooting voltammograms, it is essential m advantage of this kind of shooting is the possibility of obtaining high peaks voltammograms facilitate decoding and reduce the magnitude of the measurement error signal.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2006104582/28A RU2322665C2 (en) | 2006-02-14 | 2006-02-14 | Method of determining concentration of b6 vitamin in biology-active ingredients |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2006104582/28A RU2322665C2 (en) | 2006-02-14 | 2006-02-14 | Method of determining concentration of b6 vitamin in biology-active ingredients |
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| Publication Number | Publication Date |
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| RU2006104582A true RU2006104582A (en) | 2007-09-20 |
| RU2322665C2 RU2322665C2 (en) | 2008-04-20 |
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| Application Number | Title | Priority Date | Filing Date |
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| RU2006104582/28A RU2322665C2 (en) | 2006-02-14 | 2006-02-14 | Method of determining concentration of b6 vitamin in biology-active ingredients |
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| RU (1) | RU2322665C2 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102192946B (en) * | 2011-03-23 | 2013-07-03 | 天津市兰标电子科技发展有限公司 | Square wave volt-ampere method for detecting vitamin content in blood samples |
| RU2632629C1 (en) * | 2016-10-31 | 2017-10-06 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Астраханский государственный университет" | Pyridoxin qualitative and quantitative determination method |
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| Publication number | Publication date |
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| RU2322665C2 (en) | 2008-04-20 |
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