RU2005107579A - METHOD FOR TRANSFORMATION OF STEM CELLS OF ANIMAL SEEDS IN VIVO - Google Patents

METHOD FOR TRANSFORMATION OF STEM CELLS OF ANIMAL SEEDS IN VIVO Download PDF

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Publication number
RU2005107579A
RU2005107579A RU2005107579/13A RU2005107579A RU2005107579A RU 2005107579 A RU2005107579 A RU 2005107579A RU 2005107579/13 A RU2005107579/13 A RU 2005107579/13A RU 2005107579 A RU2005107579 A RU 2005107579A RU 2005107579 A RU2005107579 A RU 2005107579A
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RU
Russia
Prior art keywords
transformation
vivo
stem cells
testes
gene constructs
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RU2005107579/13A
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Russian (ru)
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RU2290442C2 (en
Inventor
Инна Петровна Новгородова (RU)
Инна Петровна Новгородова
Натали Анатольевна Зиновьева (RU)
Наталия Анатольевна Зиновьева
Лев Константинович Эрнст (RU)
Лев Константинович Эрнст
Original Assignee
Всероссийский государственный научно-исследовательский институт животноводства (ВИЖ) (RU)
Всероссийский государственный научно-исследовательский институт животноводства (ВИЖ)
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Application filed by Всероссийский государственный научно-исследовательский институт животноводства (ВИЖ) (RU), Всероссийский государственный научно-исследовательский институт животноводства (ВИЖ) filed Critical Всероссийский государственный научно-исследовательский институт животноводства (ВИЖ) (RU)
Priority to RU2005107579/13A priority Critical patent/RU2290442C2/en
Publication of RU2005107579A publication Critical patent/RU2005107579A/en
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Publication of RU2290442C2 publication Critical patent/RU2290442C2/en

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Claims (1)

Способ трансформации стволовых клеток семенников животных in vivo, включающий инъецирование векторных систем непосредственно в семенники и определение эффективности трансформации генных конструкций, отличающийся тем, что перед проведением биоинженерных манипуляций определяли возраст самцов с максимальным числом сперматогоний, а инъецирование векторных систем непосредственно в семенники проводили с использованием генных конструкций pX-RSVhGH и pX-Ins (гормон роста человека и инсулина человека), причем определение эффективности трансформации генных конструкций проводили при помощи иммуногистохимического набора Novocastra (Sigma, США).A method of transforming stem cells of animal testes in animals in vivo, including injecting vector systems directly into the testes and determining the efficiency of transformation of gene constructs, characterized in that before bioengineering manipulations, the age of the males with the maximum number of spermatogonia was determined, and vector systems were injected directly into the testes using gene constructs pX-RSVhGH and pX-Ins (human growth hormone and human insulin), moreover, the determination of transformation efficiency and gene constructs were carried out using immunohistochemical kit Novocastra (Sigma, USA).
RU2005107579/13A 2005-03-21 2005-03-21 Method for in vivo transformation of animal testicle stem cells RU2290442C2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU2005107579/13A RU2290442C2 (en) 2005-03-21 2005-03-21 Method for in vivo transformation of animal testicle stem cells

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Application Number Priority Date Filing Date Title
RU2005107579/13A RU2290442C2 (en) 2005-03-21 2005-03-21 Method for in vivo transformation of animal testicle stem cells

Publications (2)

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RU2005107579A true RU2005107579A (en) 2006-09-10
RU2290442C2 RU2290442C2 (en) 2006-12-27

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ629077A (en) * 2012-03-16 2015-12-24 Regeneron Pharma Non-human animals expressing ph-sensitive immunoglobulin sequences
US10940318B2 (en) 2014-06-17 2021-03-09 Morton M. Mower Method and apparatus for electrical current therapy of biological tissue

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RU2290442C2 (en) 2006-12-27

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Effective date: 20110322