RU2004136502A - METHOD OF ALLERGY DIAGNOSTICS BY INDICATIONS OF CHEMILUMINESCENT LUMINANCE OF NEUTROPHILS - Google Patents

METHOD OF ALLERGY DIAGNOSTICS BY INDICATIONS OF CHEMILUMINESCENT LUMINANCE OF NEUTROPHILS Download PDF

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Publication number
RU2004136502A
RU2004136502A RU2004136502/15A RU2004136502A RU2004136502A RU 2004136502 A RU2004136502 A RU 2004136502A RU 2004136502/15 A RU2004136502/15 A RU 2004136502/15A RU 2004136502 A RU2004136502 A RU 2004136502A RU 2004136502 A RU2004136502 A RU 2004136502A
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RU
Russia
Prior art keywords
chemiluminescence
antigen
blood
blood serum
neutrophilic leukocytes
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RU2004136502/15A
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Russian (ru)
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RU2289138C2 (en
Inventor
Тать на Анатольевна Грачева (RU)
Татьяна Анатольевна Грачева
Борис Анатольевич Рудой (RU)
Борис Анатольевич Рудой
Владимир Петрович Рассанов (RU)
Владимир Петрович Рассанов
Иль Владимирович Дармов (RU)
Илья Владимирович Дармов
Олег Евгеньевич Грачев (RU)
Олег Евгеньевич Грачев
Original Assignee
Научно-исследовательский институт микробиологии Министерства обороны Российской Федерации (RU)
Научно-исследовательский институт микробиологии Министерства обороны Российской Федерации
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Application filed by Научно-исследовательский институт микробиологии Министерства обороны Российской Федерации (RU), Научно-исследовательский институт микробиологии Министерства обороны Российской Федерации filed Critical Научно-исследовательский институт микробиологии Министерства обороны Российской Федерации (RU)
Priority to RU2004136502/15A priority Critical patent/RU2289138C2/en
Publication of RU2004136502A publication Critical patent/RU2004136502A/en
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Publication of RU2289138C2 publication Critical patent/RU2289138C2/en

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  • Investigating Or Analysing Biological Materials (AREA)

Claims (1)

Способ аллергодиагностики, включающий выделение нейтрофильных лейкоцитов из крови исследуемого, формирование in vitro иммунных комплексов из сыворотки крови и антигена, измерение уровней хемилюминесценции препаратов, отличающийся тем, что нейтрофильные лейкоциты крови выделяют в градиенте плотности разделяющей жидкости с последующей отмывкой клеток в щадящем режиме; концентрацию нейтрофильных лейкоцитов доводят до (4...6)·106 кл·мл-l, иммунные комплексы получают, смешивая сыворотку крови исследуемого и антиген (аллерген), и инкубируют их; измеряют показатели фонового значения хемилюминесценции среды инкубации, спонтанной хемилюминесценции, внесенных в пробу отмытых нейтрофилов и интенсивность хемилюминесценции после добавления в пробу комплекса сыворотки крови и исследуемого аллергена (антигена); вычисляют показатель коэффициента стимуляции; оценку достоверности отличий в показателях до и после стимуляции проводят по результатам не менее чем трехкратных повторных измерений, а в качестве среды инкубации используют следующий состав: раствор Хенкса (рН 7,4) - 0,7...0,9 мл, раствор люминола 0,1...0,5 мМ - 0,1 мл.Allergic diagnostic method, including the isolation of neutrophilic leukocytes from the blood of the subject, the formation of in vitro immune complexes from blood serum and antigen, the measurement of chemiluminescence levels of drugs, characterized in that the neutrophilic leukocytes of the blood are isolated in a density gradient separating liquid with subsequent washing of the cells in a gentle mode; the concentration of neutrophilic leukocytes is adjusted to (4 ... 6) · 10 6 cells · ml- l , the immune complexes are obtained by mixing the test blood serum and the antigen (allergen), and incubated; measures the background values of the chemiluminescence of the incubation medium, spontaneous chemiluminescence introduced into the washed neutrophil sample and the chemiluminescence intensity after adding a complex of blood serum and the test allergen (antigen) to the sample; calculate the indicator of the coefficient of stimulation; the significance of differences in the indices before and after stimulation is evaluated based on the results of at least three repeated measurements, and the following composition is used as the incubation medium: Hanks solution (pH 7.4) - 0.7 ... 0.9 ml, luminol solution 0.1 ... 0.5 mmol - 0.1 ml.
RU2004136502/15A 2004-12-14 2004-12-14 Method for allergodiagnostics by the values of chemiluminescent phosphorescence of neutrophils RU2289138C2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU2004136502/15A RU2289138C2 (en) 2004-12-14 2004-12-14 Method for allergodiagnostics by the values of chemiluminescent phosphorescence of neutrophils

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Application Number Priority Date Filing Date Title
RU2004136502/15A RU2289138C2 (en) 2004-12-14 2004-12-14 Method for allergodiagnostics by the values of chemiluminescent phosphorescence of neutrophils

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RU2004136502A true RU2004136502A (en) 2006-05-20
RU2289138C2 RU2289138C2 (en) 2006-12-10

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RU2004136502/15A RU2289138C2 (en) 2004-12-14 2004-12-14 Method for allergodiagnostics by the values of chemiluminescent phosphorescence of neutrophils

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Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EA018686B1 (en) * 2012-07-04 2013-09-30 ОБЩЕСТВО С ОГРАНИЧЕННОЙ ОТВЕТСТВЕННОСТЬЮ "ДИСофт" Method for determining functional condition of biologic tissue and use of this method for determining viability and/or necrotization rate thereof
RU2555352C1 (en) * 2013-12-12 2015-07-10 Федеральное государственное бюджетное учреждение "Научно-исследовательский институт медицинских проблем Севера" Сибирского отделения Российской академии медицинских наук (ФГБУ "НИИМПС" СО РАМН) Method of diagnosing staphylococcal allergy in case of allergic rhinitis
RU2662997C2 (en) * 2016-11-22 2018-07-31 Федеральное государственное бюджетное образовательное учреждение высшего образования "Кубанский государственный медицинский университет" Минздрава России (ФГБОУ ВО КубГМУ Минздрава России) Method for rapid evaluation of viability of cells in tissue engineering structures
BR102018015288A2 (en) * 2017-08-08 2021-11-09 Euroimmun Medizinische Labordiagnostika Ag METHOD TO PROVE A BASOPHIL ACTIVATION
CN112941025B (en) * 2021-03-25 2023-05-02 四川大学华西医院 Method and kit for separating neutrophils in blood

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Effective date: 20071215