RU2003109417A - METHOD FOR PRODUCING AN INSECTICIDE DRUG AND THE DRUG BASED ON BACILLUS THURINGIENSIS - Google Patents

METHOD FOR PRODUCING AN INSECTICIDE DRUG AND THE DRUG BASED ON BACILLUS THURINGIENSIS Download PDF

Info

Publication number
RU2003109417A
RU2003109417A RU2003109417/13A RU2003109417A RU2003109417A RU 2003109417 A RU2003109417 A RU 2003109417A RU 2003109417/13 A RU2003109417/13 A RU 2003109417/13A RU 2003109417 A RU2003109417 A RU 2003109417A RU 2003109417 A RU2003109417 A RU 2003109417A
Authority
RU
Russia
Prior art keywords
bacillus thuringiensis
adhesive
drug
filler
dissolved
Prior art date
Application number
RU2003109417/13A
Other languages
Russian (ru)
Other versions
RU2264102C2 (en
Inventor
Людмила Кирилловна Каменек (RU)
Людмила Кирилловна Каменек
Original Assignee
Людмила Кирилловна Каменек (RU)
Людмила Кирилловна Каменек
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Людмила Кирилловна Каменек (RU), Людмила Кирилловна Каменек filed Critical Людмила Кирилловна Каменек (RU)
Priority to RU2003109417/13A priority Critical patent/RU2264102C2/en
Publication of RU2003109417A publication Critical patent/RU2003109417A/en
Application granted granted Critical
Publication of RU2264102C2 publication Critical patent/RU2264102C2/en

Links

Landscapes

  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Adhesives Or Adhesive Processes (AREA)

Claims (2)

1. Способ получения инсектицидного препарата, включающий культивирование бактерий Bacillus thuringiensis, выделение из биомассы дельта-эндотоксина путем введения растворителя, удаление осадка и смешивание раствора с наполнителем и прилипателем с дальнейшим высушиванием целевого продукта, отличающийся тем, что перед выделением из биомассы дельта-эндотоксина из нее выделяют активную фракцию - осадок, который растворяют в водном растворе восстановителя в щелочи, при этом в качестве восстановителя используют тиомочевину в концентрации 0,04-0,08%, а в качестве прилипателя используют гидролизованный хитин или мелассу или сухую пахту или сыворотку или рыбий клей размолотый или пектин из свекловичного или яблочного жома.1. A method of obtaining an insecticidal preparation, comprising culturing the bacteria Bacillus thuringiensis, isolating delta endotoxin from biomass by introducing a solvent, removing sediment and mixing the solution with filler and adhesive, and then drying the target product, characterized in that before isolation of delta endotoxin from biomass an active fraction is precipitated - a precipitate, which is dissolved in an aqueous solution of a reducing agent in alkali, while thiourea at a concentration of 0.04-0.08% is used as a reducing agent, and in as an adhesive, hydrolyzed chitin or molasses or dry buttermilk or whey or fish glue or pectin from beet or apple pulp are used. 2. Инсектицидный препарат на основе бактерий Bacillus thuringiensis, содержащий в качестве действующего начала растворенный дельта-эндотоксин, а в составе целевых добавок растворитель, прилипатель-стабилизатор, наполнитель, отличающийся тем, что получен согласно способу по п.1 и имеет следующий состав, мас.%:2. An insecticidal preparation based on bacteria Bacillus thuringiensis, containing, as an active principle, dissolved delta-endotoxin, and in the composition of target additives a solvent, an adhesive stabilizer, a filler, characterized in that it is obtained according to the method according to claim 1 and has the following composition, wt .%: Действующее начало - растворенныйActive principle - dissolved дельта-эндотоксинdelta endotoxin Bacillus thuringiensis 10-20%Bacillus thuringiensis 10-20% Щелочь - NaOH или КОН 3-8%Alkali - NaOH or KOH 3-8% Восстановитель - тиомочевина 1-8%The reducing agent is thiourea 1-8% Прилипатель - стабилизатор рабочейAdhesive - stabilizer working суспензии - меласса илиsuspensions - molasses or сухая пахта или сывороткаdry buttermilk or whey или гидролизованный хитинor hydrolyzed chitin или рыбий клей размолотый илиor ground fish glue or пектин из свекловичного или яблочного жома 3-20%pectin from beet or apple pulp 3-20% Наполнитель До 100%Filler Up to 100%
RU2003109417/13A 2003-04-04 2003-04-04 Method for preparing insecticide preparation and preparation based on microorganisms bacillus thuringiensis RU2264102C2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU2003109417/13A RU2264102C2 (en) 2003-04-04 2003-04-04 Method for preparing insecticide preparation and preparation based on microorganisms bacillus thuringiensis

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2003109417/13A RU2264102C2 (en) 2003-04-04 2003-04-04 Method for preparing insecticide preparation and preparation based on microorganisms bacillus thuringiensis

Publications (2)

Publication Number Publication Date
RU2003109417A true RU2003109417A (en) 2005-01-20
RU2264102C2 RU2264102C2 (en) 2005-11-20

Family

ID=34977385

Family Applications (1)

Application Number Title Priority Date Filing Date
RU2003109417/13A RU2264102C2 (en) 2003-04-04 2003-04-04 Method for preparing insecticide preparation and preparation based on microorganisms bacillus thuringiensis

Country Status (1)

Country Link
RU (1) RU2264102C2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2514023C1 (en) * 2012-12-26 2014-04-27 Государственное научное учреждение Всероссийский научно-исследовательский институт сельскохозяйственной микробиологии Российской академии сельскохозяйственных наук (ГНУ ВНИИСХМ Россельхозакадемии) Strain bacillus thuringiensis var darmstadiensis n25 as means of integrated effect on harmful coleopteran insects and phytopathogenic fungi

Also Published As

Publication number Publication date
RU2264102C2 (en) 2005-11-20

Similar Documents

Publication Publication Date Title
US20140287919A1 (en) Modulation of plant immune system function
CN1642986A (en) Cell wall derivatives from biomass and preparation thereof
RU2011111668A (en) Stabilized Pharmaceutical Composition, Liquid Preparative Form
US11279962B2 (en) Chitin and chitosan producing methods
Humbert et al. Calcium gluconate as cross-linker improves survival and shelf life of encapsulated and dried Metarhizium brunneum and Saccharomyces cerevisiae for the application as biological control agents
CN108976306A (en) A method of extracting separating polyose from kelp
RU2003109418A (en) METHOD FOR PRODUCING AN INSECTICIDE DRUG AND THE DRUG BASED ON BACILLUS THURINGIENSIS
Thakur et al. An overview of fungal chitinases and their potential applications
RU2003109417A (en) METHOD FOR PRODUCING AN INSECTICIDE DRUG AND THE DRUG BASED ON BACILLUS THURINGIENSIS
CN106497791A (en) A kind of staphylococcus aureuses lyophilizing is prepared and uses NEW TYPE OF COMPOSITE protective agent
RU2003109416A (en) METHOD FOR PRODUCING AN INSECTICIDE DRUG AND THE DRUG BASED ON BACILLUS THURINGIENSIS
RU2003109415A (en) METHOD FOR PRODUCING AN INSECTICIDE DRUG AND THE DRUG BASED ON BACILLUS THURINGIENSIS
CN103965518A (en) Edible maize starch preservative film and preparation method thereof
CN106751281A (en) A kind of bentonite deodorant containing medical stone/PVA sterilization fresh-keeping films and preparation method thereof
CA2938065C (en) Compositions and methods for directing the oviposition of mosquitoes
CN104782754A (en) Fruit and vegetable fresh-keeping agent containing peony seed meal water extract as well as production method and application of fresh-keeping agent
GB2539744B (en) Chitinolytic enzymes for use in the treatment of sea lice infestations
CN103583528A (en) Germicidal aqueous suspension containing beta-cyclodextrin and preparation method thereof
Lárez-Velásquez Chitosan: an overview of its multiple advantages for creating sustainable development poles
ES2916102T3 (en) Method for obtaining at least one or more beta-glucan compounds or a solid suspension containing beta-glucan from yeast cells
JP2008154456A (en) Proteolytic enzyme
CN104356173A (en) Method for preparing chitosan oligose from fly grub shells
CN103444782A (en) Efficient biological insecticide
RU2012101021A (en) AGRICULTURAL PLANT GROWTH biostimulator from chitin of crustaceans and a method of producing a growth biostimulant of agricultural plants from crustacean chitin
LU501445B1 (en) Carboxymethyl Chitosan Schiff Base and its Preparation and Application

Legal Events

Date Code Title Description
MM4A The patent is invalid due to non-payment of fees

Effective date: 20160405