RU2003109416A - METHOD FOR PRODUCING AN INSECTICIDE DRUG AND THE DRUG BASED ON BACILLUS THURINGIENSIS - Google Patents

METHOD FOR PRODUCING AN INSECTICIDE DRUG AND THE DRUG BASED ON BACILLUS THURINGIENSIS Download PDF

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Publication number
RU2003109416A
RU2003109416A RU2003109416/13A RU2003109416A RU2003109416A RU 2003109416 A RU2003109416 A RU 2003109416A RU 2003109416/13 A RU2003109416/13 A RU 2003109416/13A RU 2003109416 A RU2003109416 A RU 2003109416A RU 2003109416 A RU2003109416 A RU 2003109416A
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RU
Russia
Prior art keywords
bacillus thuringiensis
adhesive
filler
dissolved
reducing agent
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RU2003109416/13A
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Russian (ru)
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RU2264101C2 (en
Inventor
Людмила Кирилловна Каменек (RU)
Людмила Кирилловна Каменек
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Людмила Кирилловна Каменек (RU)
Людмила Кирилловна Каменек
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Priority to RU2003109416/13A priority Critical patent/RU2264101C2/en
Publication of RU2003109416A publication Critical patent/RU2003109416A/en
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Publication of RU2264101C2 publication Critical patent/RU2264101C2/en

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  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Adhesives Or Adhesive Processes (AREA)

Claims (2)

1. Способ получения инсектицидного препарата, включающий культивирование бактерий Bacillus thuringiensis, выделение из биомассы дельта-эндотоксина путем введения растворителя, удаление осадка и смешивание раствора с наполнителем и прилипателем с дальнейшим высушиванием целевого продукта, отличающийся тем, что перед выделением из биомассы дельта-эндотоксина из нее выделяют активную фракцию - осадок, который растворяют в водном растворе восстановителя в щелочи, при этом в качестве восстановителя используют β-меркаптоэтонол в концентрации 0,04-0,08%, а в качестве прилипателя используют гидролизованный хитин или мелассу или сухую пахту или сыворотку или рыбий клей размолотый или пектин из свекловичного или яблочного жома.1. A method of obtaining an insecticidal preparation, comprising culturing the bacteria Bacillus thuringiensis, isolating delta endotoxin from biomass by introducing a solvent, removing sediment and mixing the solution with filler and adhesive, and then drying the target product, characterized in that before isolation of delta endotoxin from biomass an active fraction is isolated - a precipitate, which is dissolved in an aqueous solution of a reducing agent in alkali, while β-mercaptoethonol in a concentration of 0.04-0.08% is used as a reducing agent, as used adhesive hydrolyzed chitin or molasses or dry buttermilk or whey or isinglass or ground beet pectin or apple pulp. 2. Инсектицидный препарат на основе бактерий Bacillus thuringiensis, содержащий в качестве действующего начала растворенный дельта-эндотоксин, а в составе целевых добавок растворитель, прилипатель-стабилизатор, наполнитель, отличающийся тем, что получен согласно способу по п.1 и имеет следующий состав, мас.%:2. An insecticidal preparation based on bacteria Bacillus thuringiensis, containing, as an active principle, dissolved delta-endotoxin, and in the composition of target additives a solvent, an adhesive stabilizer, a filler, characterized in that it is obtained according to the method according to claim 1 and has the following composition, wt .%: Действующее начало - растворенныйActive principle - dissolved дельта-эндотоксинdelta endotoxin Bacillus thuringiensis 10-20%Bacillus thuringiensis 10-20% Щелочь - NaOH или КОН 3-8%Alkali - NaOH or KOH 3-8% Восстановитель - β-меркаптоэтонол 1-8%The reducing agent is β-mercaptoetonol 1-8% Прилипатель - стабилизатор рабочейAdhesive - stabilizer working суспензии - меласса или сухая пахта илиsuspensions - molasses or dry buttermilk or сывороткаserum или гидролизованный хитинor hydrolyzed chitin или рыбий клей размолотый илиor ground fish glue or пектин из свекловичного или яблочного жома 3-20%pectin from beet or apple pulp 3-20% Наполнитель До 100%Filler Up to 100%
RU2003109416/13A 2003-04-04 2003-04-04 Method for preparing insecticide preparation and preparation based on microorganisms bacillus thuringiensis RU2264101C2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU2003109416/13A RU2264101C2 (en) 2003-04-04 2003-04-04 Method for preparing insecticide preparation and preparation based on microorganisms bacillus thuringiensis

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2003109416/13A RU2264101C2 (en) 2003-04-04 2003-04-04 Method for preparing insecticide preparation and preparation based on microorganisms bacillus thuringiensis

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RU2003109416A true RU2003109416A (en) 2005-01-20
RU2264101C2 RU2264101C2 (en) 2005-11-20

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RU2003109416/13A RU2264101C2 (en) 2003-04-04 2003-04-04 Method for preparing insecticide preparation and preparation based on microorganisms bacillus thuringiensis

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Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2603271C1 (en) * 2015-07-22 2016-11-27 Федеральное государственное бюджетное научное учреждение "Дальневосточный зональный научно-исследовательский ветеринарный институт" (ФГБНУ ДальЗНИВИ) Bioinsecticide for controlling large bee moth

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Effective date: 20160405