RU2001121697A - New mutant carbamoyl phosphate synthetase and method for the production of carbamoyl phosphate derivative compounds - Google Patents

New mutant carbamoyl phosphate synthetase and method for the production of carbamoyl phosphate derivative compounds

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Publication number
RU2001121697A
RU2001121697A RU2001121697/13A RU2001121697A RU2001121697A RU 2001121697 A RU2001121697 A RU 2001121697A RU 2001121697/13 A RU2001121697/13 A RU 2001121697/13A RU 2001121697 A RU2001121697 A RU 2001121697A RU 2001121697 A RU2001121697 A RU 2001121697A
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RU
Russia
Prior art keywords
synthetase
carbamoyl phosphate
uridine
monophosphate
seq
Prior art date
Application number
RU2001121697/13A
Other languages
Russian (ru)
Other versions
RU2264459C2 (en
Inventor
Леонид Романович Птицын
Сергей Васильевич Смирнов
Ирина Борисовна Альтман
Анна Евгеньевна Новикова
Вероника Александровна КОТЛЯРОВА
Михаил Маркович Гусятинер
Юлия Георгиевна Ростова
Татьяна Абрамовна Ямпольская
Original Assignee
Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика"
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Publication date
Application filed by Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" filed Critical Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика"
Priority to RU2001121697/13A priority Critical patent/RU2264459C2/en
Priority claimed from RU2001121697/13A external-priority patent/RU2264459C2/en
Priority to JP2002221111A priority patent/JP4207484B2/en
Priority to US10/210,115 priority patent/US6991924B2/en
Priority to BR0202976-6A priority patent/BR0202976A/en
Publication of RU2001121697A publication Critical patent/RU2001121697A/en
Priority to US11/253,665 priority patent/US7211419B2/en
Application granted granted Critical
Publication of RU2264459C2 publication Critical patent/RU2264459C2/en

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Claims (10)

1. Карбамоилфосфатсинтетаза, отличающаяся тем, что последовательность аминокислот, соответствующая положениям с 947 по 951 в последовательности SEQ ID NO:20, заменена любой из последовательностей аминокислот SEQ ID NO:l - SEQ ID NO:9, и ингибирование уридин-5’-монофосфатом по типу обратной связи в указанной карбамоилфосфатсинтетазе ослаблено.1. Carbamoyl phosphate synthetase, characterized in that the amino acid sequence corresponding to positions 947 to 951 in the sequence of SEQ ID NO: 20 is replaced by any of the amino acid sequences of SEQ ID NO: l to SEQ ID NO: 9, and inhibition by uridine-5'-monophosphate feedback type in the specified carbamoylphosphate synthetase is weakened. 2. Карбамоилфосфатсинтетаза по п.1, отличающаяся тем, что указаная карбамоилфосфатсинтетаза является карбамоилфосфатсинтетазой из Escherichia coli.2. The carbamoyl phosphate synthetase according to claim 1, characterized in that said carbamoyl phosphate synthetase is carbamoyl phosphate synthetase from Escherichia coli. 3. Карбамоилфосфатсинтетаза по п.1, отличающаяся тем, что последовательность аминокислот в положении с 947 по 951 в последовательности SEQ ID NO:20 заменена любой из последовательностей аминокислот SEQ ID NO:l - SEQ ID NO:9, и ингибирование уридин-5'-монофосфатом по типу обратной связи в указанной карбамоилфосфатсинтетазе ослаблено.3. The carbamoyl phosphate synthetase according to claim 1, characterized in that the amino acid sequence at position 947 to 951 in the sequence of SEQ ID NO: 20 is replaced by any of the amino acid sequences of SEQ ID NO: l to SEQ ID NO: 9, and the inhibition of uridine-5 ' -monophosphate feedback type in the specified carbamoylphosphate synthetase is weakened. 4. Карбамоилфосфатсинтетаза по п.1, отличающаяся тем, что указаная карбамоилфосфатсинтетаза включает делеции, замены, вставки или добавления одной или нескольких аминокислот в одном или нескольких положениях, кроме положений с 947 по 951, и ингибирование уридин-5’-монофосфатом по типу обратной связи в указанной карбамоилфосфатсинтетазе ослаблено.4. Carbamoylphosphate synthetase according to claim 1, characterized in that said carbamoylphosphate synthetase includes deletions, substitutions, insertions or additions of one or more amino acids in one or more positions, except positions 947 to 951, and inhibition of uridine-5'-monophosphate by the reverse type bonds in the specified carbamoylphosphate synthetase are weakened. 5. ДНК, кодирующая карбамоилфосфатсинтетазу по любому из пп.1-4.5. DNA encoding a carbamoylphosphate synthetase according to any one of claims 1 to 4. 6. Бактерия, принадлежащая к роду Escherichia, отличающаяся тем, что указанная бактерия трансформирована с помощью ДНК по п.5.6. A bacterium belonging to the genus Escherichia, characterized in that the bacterium is transformed using DNA according to claim 5. 7. Бактерия по п.6, отличающаяся тем, что указанная бактерия обладает способностью к продукции соединения, выбранного из группы, состоящей из L-аргинина, цитруллина и производных пиримидина.7. The bacterium according to claim 6, characterized in that said bacterium is capable of producing a compound selected from the group consisting of L-arginine, citrulline and pyrimidine derivatives. 8. Бактерия по 7, отличающаяся тем, что производными пиримидина являются оротовая кислота, уридин, уридин-5’-монофосфат, цитадин и цитидин-5’-монофосфат.8. The bacterium according to claim 7, characterized in that the derivatives of pyrimidine are orotic acid, uridine, uridine-5’-monophosphate, cytadine and cytidine-5’-monophosphate. 9. Способ продукции соединения, выбранного из группы, состоящей из L-аргинина, цитруллина и производных пиримидина, включающий стадии: выращивания бактерии по любому из пп.6-8 в питательной среде с целью продукции и накопления указанного соединения в питательной среде, и выделения указанного соединения из культуральной жидкости.9. A method for producing a compound selected from the group consisting of L-arginine, citrulline and pyrimidine derivatives, comprising the steps of: growing a bacterium according to any one of claims 6-8 in a nutrient medium for the purpose of producing and accumulating said compound in a nutrient medium, and isolating the specified compound from the culture fluid. 10. Способ по п.9, отличающийся тем, что производными пиримидина являются оротовая кислота, уридин, уридин-5’-монофосфат, цитидин и цитидин-5’-монофосфат.10. The method according to claim 9, characterized in that the derivatives of pyrimidine are orotic acid, uridine, uridine-5’-monophosphate, cytidine and cytidine-5’-monophosphate.
RU2001121697/13A 2001-08-03 2001-08-03 New mutant carbamoyl-phosphate synthase and method for production of carbamoyl-phosphate derivatives RU2264459C2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
RU2001121697/13A RU2264459C2 (en) 2001-08-03 2001-08-03 New mutant carbamoyl-phosphate synthase and method for production of carbamoyl-phosphate derivatives
JP2002221111A JP4207484B2 (en) 2001-08-03 2002-07-30 Novel mutant carbamoyl phosphate synthetase and method for producing compound derived from carbamoyl phosphate
US10/210,115 US6991924B2 (en) 2001-08-03 2002-08-02 Mutant carbamoylphosphate synthetase and method for producing compounds derived from carbamoylphosphate
BR0202976-6A BR0202976A (en) 2001-08-03 2002-08-02 Large subunit of carbamoyl phosphate synthetase, carbamoyl phosphate synthetase, DNA, bacteria, and process to produce compound
US11/253,665 US7211419B2 (en) 2001-08-03 2005-10-20 Mutant carbamoylphosphate synthetase and method for producing compounds derived from carbamoylphosphate

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2001121697/13A RU2264459C2 (en) 2001-08-03 2001-08-03 New mutant carbamoyl-phosphate synthase and method for production of carbamoyl-phosphate derivatives

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RU2001121697A true RU2001121697A (en) 2003-07-10
RU2264459C2 RU2264459C2 (en) 2005-11-20

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US (2) US6991924B2 (en)
JP (1) JP4207484B2 (en)
BR (1) BR0202976A (en)
RU (1) RU2264459C2 (en)

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RU2230114C2 (en) * 2001-11-30 2004-06-10 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" Mutant glutamine synthetase, dna fragment, strain of escherichia coli as p roducer of l-glutamine and method for preparing l-amino acids
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RU2013140115A (en) 2013-08-30 2015-03-10 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО "АГРИ") METHOD FOR PRODUCING L-AMINO ACIDS USING THE BACTERIA OF THE Enterobacteriaceae FAMILY IN WHICH THE EXPRESSION OF THE znuACB GENERAL CLUSTER IS DISORDERED
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