RU2001117332A - The method of obtaining L-methionine, a strain of the bacterium Escherichia coli VKPM B-8125 - producer of L-methionine - Google Patents

The method of obtaining L-methionine, a strain of the bacterium Escherichia coli VKPM B-8125 - producer of L-methionine

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Publication number
RU2001117332A
RU2001117332A RU2001117332/13A RU2001117332A RU2001117332A RU 2001117332 A RU2001117332 A RU 2001117332A RU 2001117332/13 A RU2001117332/13 A RU 2001117332/13A RU 2001117332 A RU2001117332 A RU 2001117332A RU 2001117332 A RU2001117332 A RU 2001117332A
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RU
Russia
Prior art keywords
bacterium
methionine
strain
escherichia coli
resistant
Prior art date
Application number
RU2001117332/13A
Other languages
Russian (ru)
Other versions
RU2209248C2 (en
Inventor
Михаил Харисович Зиятдинов
Эльвира Борисовна Ворошилова
Михаил Маркович Гусятинер
Original Assignee
Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика"
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Publication date
Application filed by Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" filed Critical Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика"
Priority to RU2001117332A priority Critical patent/RU2209248C2/en
Priority claimed from RU2001117332A external-priority patent/RU2209248C2/en
Publication of RU2001117332A publication Critical patent/RU2001117332A/en
Application granted granted Critical
Publication of RU2209248C2 publication Critical patent/RU2209248C2/en

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Claims (8)

1. Бактерия, принадлежащая к роду Escherichia, обладающая способностью к продукции по крайней мере 0,5 г/л L-метионина в процессе выращивания на минимальной среде и устойчивая к аналогу метионина.1. A bacterium belonging to the genus Escherichia, with the ability to produce at least 0.5 g / l of L-methionine during cultivation on minimal medium and resistant to the methionine analogue. 2. Бактерия по п.1, отличающаяся тем, что указанная бактерия является устойчивой к норлейцину.2. The bacterium according to claim 1, characterized in that the bacterium is resistant to norleucine. 3. Бактерия по п.2, отличающаяся тем, что указанная бактерия получена путем селекции штамма, устойчивого к норлейцину, из бактерии, принадлежащей к роду Escherichia, причем указанная селекция осуществлена по крайней мере один раз для указанного аналога метионина.3. The bacterium according to claim 2, characterized in that said bacterium is obtained by selection of a strain resistant to norleucine from a bacterium belonging to the genus Escherichia, said selection being carried out at least once for said methionine analogue. 4. Бактерия по любому из пп.1-3, отличающаяся тем, что указанная бактерия получена из бактерии, устойчивой к L- гомосерину.4. The bacterium according to any one of claims 1 to 3, characterized in that said bacterium is obtained from a bacterium resistant to L-homoserine. 5. Бактерия по любому из пп.1-4, отличающаяся тем, что указанная бактерия является бактерией Escherichia coli.5. The bacterium according to any one of claims 1 to 4, characterized in that said bacterium is an Escherichia coli bacterium. 6. Бактерия по п.5, отличающаяся тем, что указанная бактерия является производной от штамма Escherichia coli C600 (Leu+).6. The bacterium according to claim 5, characterized in that the bacterium is derived from the strain Escherichia coli C600 (Leu + ). 7. Бактерия по п.6, отличающаяся тем, что указанная бактерия представляет собой штамм Escherichia coli ВКПМ В-8125.7. The bacterium according to claim 6, characterized in that said bacterium is a strain of Escherichia coli VKPM B-8125. 8. Способ получения L-метионина, включающий стадии выращивания бактерии по любому из пп.1-7 в питательной среде с целью продукции и накопления в питательной среде L-метионина, и выделения L-метионина из культуральной жидкости.8. A method for producing L-methionine, comprising the steps of growing the bacterium according to any one of claims 1 to 7 in a nutrient medium for the purpose of production and accumulation of L-methionine in a nutrient medium, and the isolation of L-methionine from the culture fluid.
RU2001117332A 2001-06-26 2001-06-26 Method for preparing l-methionine, strain of bacterium escherichia coli vkpm b-8125 as producer of l-methionine RU2209248C2 (en)

Priority Applications (1)

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FR2862067A1 (en) * 2003-11-06 2005-05-13 Metabolic Explorer Sa New evolved microorganisms with altered metabolic pathways, useful e.g. for production of amino acids, are selected as mutants able to grow on defined media
RU2009136544A (en) 2009-10-05 2011-04-10 Закрытое акционерное общество "Научно-исследовательский институт "Аджиномото-Генетика" (ЗАО АГРИ) (RU) METHOD FOR PRODUCING L-CISTEINE USING THE ENTEROBACTERIACEAE FAMILY BACTERIA
JP5817529B2 (en) 2009-11-30 2015-11-18 味の素株式会社 L-cysteine producing bacterium and method for producing L-cysteine
WO2012036151A1 (en) 2010-09-14 2012-03-22 味の素株式会社 Sulfur amino acid-producing bacteria and method for producing sulfur amino acids
WO2012091479A2 (en) * 2010-12-29 2012-07-05 Cj Cheiljedang Corporation Methods for production of l-methionine and related products
JP2014087259A (en) 2011-02-22 2014-05-15 Ajinomoto Co Inc L-cysteine-producing bacterium, and production method of l-cysteine
JP6020443B2 (en) 2011-04-01 2016-11-02 味の素株式会社 Method for producing L-cysteine
RU2013118637A (en) 2013-04-23 2014-10-27 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО "АГРИ") METHOD FOR PRODUCING L-AMINO ACIDS USING THE BACTERIA OF THE ENTEROBACTERIACEAE FAMILY IN WHICH THE yjjK GENE IS ADJUSTABLE
CN104736707B (en) 2013-10-21 2017-08-25 味之素株式会社 The method for producing L amino acid
RU2014105547A (en) 2014-02-14 2015-08-20 Адзиномото Ко., Инк. METHOD FOR PRODUCING L-AMINO ACIDS USING THE ENTEROBACTERIACEAE FAMILY HAVING A SUPER EXPRESSED GENE yajL
RU2015120052A (en) 2015-05-28 2016-12-20 Аджиномото Ко., Инк. A method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family in which gshA gene expression is weakened
JP7066977B2 (en) 2017-04-03 2022-05-16 味の素株式会社 Manufacturing method of L-amino acid
WO2020067487A1 (en) 2018-09-28 2020-04-02 Ajinomoto Co., Inc. Method for producing l-methionine using a bacterium
EP3861109A1 (en) 2018-10-05 2021-08-11 Ajinomoto Co., Inc. Method for producing target substance by bacterial fermentation
JP2022521544A (en) 2019-02-22 2022-04-08 味の素株式会社 Method for producing L-amino acid using a bacterium belonging to the family Enterobacteriaceae that overexpresses the ydiJ gene
JP2022526181A (en) 2019-04-05 2022-05-23 味の素株式会社 Method for producing L-amino acid
JP2022550084A (en) 2019-09-25 2022-11-30 味の素株式会社 Method for producing L-amino acid by bacterial fermentation

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