RU2000129650A - BACTERIOLYTIC COMPLEX, METHOD FOR ITS OBTAINING AND STRAIN FOR IMPLEMENTING THE METHOD - Google Patents
BACTERIOLYTIC COMPLEX, METHOD FOR ITS OBTAINING AND STRAIN FOR IMPLEMENTING THE METHODInfo
- Publication number
- RU2000129650A RU2000129650A RU2000129650/13A RU2000129650A RU2000129650A RU 2000129650 A RU2000129650 A RU 2000129650A RU 2000129650/13 A RU2000129650/13 A RU 2000129650/13A RU 2000129650 A RU2000129650 A RU 2000129650A RU 2000129650 A RU2000129650 A RU 2000129650A
- Authority
- RU
- Russia
- Prior art keywords
- strain
- complex
- bacteriolytic
- xanthomonas campestris
- bacterium
- Prior art date
Links
- 230000001420 bacteriolysis Effects 0.000 title claims 5
- 241000589636 Xanthomonas campestris Species 0.000 claims 4
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims 4
- BFNBIHQBYMNNAN-UHFFFAOYSA-N Ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims 2
- 241000894006 Bacteria Species 0.000 claims 2
- 229940041514 Candida albicans extract Drugs 0.000 claims 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N D-Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims 2
- 239000001888 Peptone Substances 0.000 claims 2
- 108010080698 Peptones Proteins 0.000 claims 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims 2
- 235000011130 ammonium sulphate Nutrition 0.000 claims 2
- 239000012531 culture fluid Substances 0.000 claims 2
- 239000008103 glucose Substances 0.000 claims 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims 2
- 235000015097 nutrients Nutrition 0.000 claims 2
- 235000019319 peptone Nutrition 0.000 claims 2
- 239000001103 potassium chloride Substances 0.000 claims 2
- 235000011164 potassium chloride Nutrition 0.000 claims 2
- 239000011734 sodium Substances 0.000 claims 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 2
- 239000012138 yeast extract Substances 0.000 claims 2
- 239000002028 Biomass Substances 0.000 claims 1
- 102000033147 ERVK-25 Human genes 0.000 claims 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L Iron(II) sulfate Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims 1
- 102000016943 Muramidase Human genes 0.000 claims 1
- 108010014251 Muramidase Proteins 0.000 claims 1
- 108091005771 Peptidases Proteins 0.000 claims 1
- 239000004365 Protease Substances 0.000 claims 1
- 241001481789 Rupicapra Species 0.000 claims 1
- 241000589634 Xanthomonas Species 0.000 claims 1
- 239000002253 acid Substances 0.000 claims 1
- 150000001412 amines Chemical class 0.000 claims 1
- 238000000502 dialysis Methods 0.000 claims 1
- 239000011790 ferrous sulphate Substances 0.000 claims 1
- 235000003891 ferrous sulphate Nutrition 0.000 claims 1
- 238000004108 freeze drying Methods 0.000 claims 1
- 150000004676 glycans Polymers 0.000 claims 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims 1
- 239000011777 magnesium Substances 0.000 claims 1
- 229910052749 magnesium Inorganic materials 0.000 claims 1
- 235000001055 magnesium Nutrition 0.000 claims 1
- 229910052757 nitrogen Inorganic materials 0.000 claims 1
- 125000002467 phosphate group Chemical class [H]OP(=O)(O[H])O[*] 0.000 claims 1
- 229920001282 polysaccharide Polymers 0.000 claims 1
- 239000005017 polysaccharide Substances 0.000 claims 1
- 150000004804 polysaccharides Polymers 0.000 claims 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N potassium Chemical class [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims 1
- 239000011591 potassium Chemical class 0.000 claims 1
- 229910052700 potassium Chemical class 0.000 claims 1
- 239000002244 precipitate Substances 0.000 claims 1
- 230000001376 precipitating Effects 0.000 claims 1
- 238000001556 precipitation Methods 0.000 claims 1
- 239000000047 product Substances 0.000 claims 1
- KEAYESYHFKHZAL-UHFFFAOYSA-N sodium Chemical class [Na] KEAYESYHFKHZAL-UHFFFAOYSA-N 0.000 claims 1
- 229910052708 sodium Inorganic materials 0.000 claims 1
Claims (3)
Глюкоза - 2-6
Пептон - 1,5-6
Дрожжевой экстракт - 1-6
Или дрожжевой автолизат с аминным азотом 440-490 мг/л - 40-300 мл
Na2HPO412H2O- - 4-5
КН2PO4 - 1-2
MgSO4•7H2O - 4-6
KCl - 0,4-1,6
FеSO4•7Н2О - 0,05-0,2
Вода - До 1 л
осаждение комплекса сульфатом аммония проводят непосредственно из культуральной жидкости.2. A method of obtaining a bacteriolytic complex, including cultivating a strain of the bacterium of the species Xanthomonas campestris on a nutrient medium, separating the biomass from the culture fluid by centrifuging, precipitating the complex with ammonium sulfate, dialysis the dissolved precipitate and freeze drying the target product, characterized in that the strain of Xanthomonas campestris is cultured. -2249 D, and as a nutrient medium using a medium containing glucose, peptone, yeast extract or yeast autolysate, phosphate salts of sodium and potassium, chamois acid magnesium, potassium chloride, ferrous sulphate and water in the following component ratio, g / l:
Glucose - 2-6
Peptone - 1.5-6
Yeast Extract - 1-6
Or yeast autolysate with amine nitrogen 440-490 mg / l - 40-300 ml
Na 2 HPO 4 12H 2 O- - 4-5
KN 2 PO 4 - 1-2
MgSO 4 • 7H 2 O - 4-6
KCl - 0.4-1.6
FeSO 4 • 7H 2 O - 0.05-0.2
Water - Up to 1 l
precipitation of the complex with ammonium sulfate is carried out directly from the culture fluid.
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
RU2000129650/13A RU2193063C2 (en) | 2000-11-29 | 2000-11-29 | Bacteriolytic complex, method of its preparing and strain for method realization |
KR1020027009664A KR20020073189A (en) | 2000-11-29 | 2001-11-28 | Bacteriolytic complex, method for producing said complex and strain for carrying out said method |
CNB018042473A CN1266271C (en) | 2000-11-29 | 2001-11-28 | Bacteriolytic complex, method for producing said complex and strain for carrying out said method |
US10/181,498 US7150985B2 (en) | 2000-11-29 | 2001-11-28 | Bacteriolytic complex, method for producing said complex and strain for carrying out said method |
PCT/RU2001/000514 WO2002044352A1 (en) | 2000-11-29 | 2001-11-28 | Bacteriolytic complex, method for producing said complex and strain for carrying out said method. |
US11/247,553 US20060051336A1 (en) | 2000-11-29 | 2005-10-11 | Bacteriolytic complex, method of its production, and strain for realization of the method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
RU2000129650/13A RU2193063C2 (en) | 2000-11-29 | 2000-11-29 | Bacteriolytic complex, method of its preparing and strain for method realization |
Publications (2)
Publication Number | Publication Date |
---|---|
RU2000129650A true RU2000129650A (en) | 2002-11-10 |
RU2193063C2 RU2193063C2 (en) | 2002-11-20 |
Family
ID=20242623
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
RU2000129650/13A RU2193063C2 (en) | 2000-11-29 | 2000-11-29 | Bacteriolytic complex, method of its preparing and strain for method realization |
Country Status (5)
Country | Link |
---|---|
US (2) | US7150985B2 (en) |
KR (1) | KR20020073189A (en) |
CN (1) | CN1266271C (en) |
RU (1) | RU2193063C2 (en) |
WO (1) | WO2002044352A1 (en) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
RU2296576C2 (en) * | 2005-05-18 | 2007-04-10 | Институт биохимии и физиологии микроорганизмов им. Г.К. Скрябина РАН | AGENT OF BACTERICIDE ACTION IN RELATES TO VEGETATIVE AND SPORE CELLS Bacillus anthracis, METHOD FOR MALIGNANT ANTHRAX PROPHYLAXIS AND TREATMENT |
EP2790514A1 (en) * | 2011-12-14 | 2014-10-22 | Fraunhofer Gesellschaft zur Förderung der angewandten Forschung e.V. | Process for degrading a biofilm on surfaces of objects |
RU2744107C1 (en) * | 2020-10-05 | 2021-03-02 | Общество с ограниченной ответственностью «Газпромнефть Научно-Технический Центр» | Xanthomonas fuscans bacterial strain - producer of xanthan gum |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5612435B1 (en) * | 1971-03-29 | 1981-03-20 | ||
GB1353877A (en) | 1972-10-12 | 1974-05-22 | Nat Food Research Inst Directo | Lytic enzymes |
SU519470A1 (en) | 1973-09-28 | 1976-06-30 | Днепропетровский Ордена Трудового Красного Знамени Государственный Университет Им.300-Летия Воссоединения Украины С Россией | Method for producing lytic enzymes |
RU1549227C (en) | 1984-10-04 | 1995-12-20 | Институт биохимии и физиологии микроорганизмов РАН | Method for producing a complex of lytic enzymes |
SU1755581A1 (en) | 1990-11-05 | 1995-08-20 | Институт Биохимии И Физиологии Микроорганизмов Ан Ссср | Method for isolation of complex of lytic ferments |
SU1774658A1 (en) | 1990-11-05 | 1995-09-10 | Институт Биохимии И Физиологии Микроорганизмов Ан Ссср | Method for production of complex of lytic ferments |
-
2000
- 2000-11-29 RU RU2000129650/13A patent/RU2193063C2/en active
-
2001
- 2001-11-28 KR KR1020027009664A patent/KR20020073189A/en not_active Application Discontinuation
- 2001-11-28 WO PCT/RU2001/000514 patent/WO2002044352A1/en active Application Filing
- 2001-11-28 US US10/181,498 patent/US7150985B2/en not_active Expired - Lifetime
- 2001-11-28 CN CNB018042473A patent/CN1266271C/en not_active Expired - Fee Related
-
2005
- 2005-10-11 US US11/247,553 patent/US20060051336A1/en not_active Abandoned
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