RU2000115708A - METHOD FOR MEASURING CONCENTRATION OF ANALYZED SUBSTANCE (OPTIONS), MEASURING INSTRUMENT FOR MEASURING CONCENTRATION OF ANALYZED SUBSTANCE - Google Patents

METHOD FOR MEASURING CONCENTRATION OF ANALYZED SUBSTANCE (OPTIONS), MEASURING INSTRUMENT FOR MEASURING CONCENTRATION OF ANALYZED SUBSTANCE

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Publication number
RU2000115708A
RU2000115708A RU2000115708/28A RU2000115708A RU2000115708A RU 2000115708 A RU2000115708 A RU 2000115708A RU 2000115708/28 A RU2000115708/28 A RU 2000115708/28A RU 2000115708 A RU2000115708 A RU 2000115708A RU 2000115708 A RU2000115708 A RU 2000115708A
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RU
Russia
Prior art keywords
sample
electrical response
measuring
predetermined
voltage
Prior art date
Application number
RU2000115708/28A
Other languages
Russian (ru)
Other versions
RU2238548C2 (en
Inventor
Тимоти Дж. ОХАРА
Мария ТЕОДОРЧИК
Махиар З. КЕРМАНИ
Original Assignee
Лайфскен, Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/333,793 external-priority patent/US6193873B1/en
Application filed by Лайфскен, Инк. filed Critical Лайфскен, Инк.
Publication of RU2000115708A publication Critical patent/RU2000115708A/en
Application granted granted Critical
Publication of RU2238548C2 publication Critical patent/RU2238548C2/en

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Claims (15)

1. Способ измерения концентрации анализируемого вещества в пробе биологической жидкости, нанесенной на полоску для электрохимической диагностики, включающую в себя наложенные друг на друга рабочий и контрольный электроды, при котором подводят заранее определенный постоянный ток источника между рабочим и контрольным электродами, контролируют разность потенциалов на электродах, наносят пробу на полоску, определяют время обнаружения пробы, отмечая, когда разность потенциалов падает ниже заранее определенного порогового напряжения, прикладывают заранее определенное постоянное напряжение к пробе, измеряют электрический отклик в заранее определенное время после приложения постоянного напряжения и вычисляют концентрацию анализируемого вещества с помощью измеренного электрического отклика.1. A method of measuring the concentration of an analyte in a sample of biological fluid deposited on a strip for electrochemical diagnostics, including working and control electrodes superimposed on each other, in which a predetermined direct current of the source between the working and control electrodes is supplied, and the potential difference on the electrodes is controlled , apply the sample to the strip, determine the detection time of the sample, noting when the potential difference falls below a predetermined threshold voltage, at put a predetermined constant voltage to the sample, measure the electrical response at a predetermined time after applying a constant voltage and calculate the concentration of the analyte using the measured electrical response. 2. Способ по п. 1, в котором измеряемым электрическим откликом является ток, протекающий через пробу в заранее определенное время. 2. The method according to claim 1, in which the measured electrical response is the current flowing through the sample at a predetermined time. 3. Способ по п. 1, в котором измеряемым электрическим откликом является заряд, проходящий через пробу от времени обнаружения пробы до заранее определенного времени. 3. The method according to p. 1, in which the measured electrical response is a charge passing through the sample from the time of detection of the sample to a predetermined time. 4. Способ по п. 1, в котором дополнительно прикладывают второе заранее определенное напряжение после заранее определенного времени и измеряют второй электрический отклик после приложения второго заранее определенного напряжения. 4. The method of claim 1, further comprising applying a second predetermined voltage after a predetermined time and measuring a second electrical response after applying the second predetermined voltage. 5. Способ по п. 4, в котором вторым электрическим откликом является скорость затухания тока, протекающего через пробу. 5. The method of claim 4, wherein the second electrical response is the decay rate of the current flowing through the sample. 6. Способ по п. 4, в котором вторым электрическим откликом является заряд, проходящий через пробу в течение заранее определенного интервала времени после приложения второго напряжения. 6. The method of claim 4, wherein the second electrical response is a charge passing through the sample for a predetermined time interval after the second voltage is applied. 7. Измерительный прибор для измерения концентрации анализируемого вещества в пробе биологической жидкости, нанесенной между рабочим и контрольным электродами диагностической полоски, содержащей связанные электрически средство для подвода заранее определенного тока между рабочим и контрольным электродами, средство для контролирования разности потенциалов на электродах, средство для определения, когда разность потенциалов падает ниже заранее определенного порогового напряжения, для индикации обнаружения пробы, средство для приложения заранее определенного постоянного напряжения к пробе в ответ на обнаружение пробы, средство для измерения результирующего электрического отклика и средство для вычисления концентрации анализируемого вещества с помощью измеренного электрического отклика. 7. A measuring device for measuring the concentration of the analyte in a sample of biological fluid deposited between the working and control electrodes of a diagnostic strip containing electrically coupled means for supplying a predetermined current between the working and control electrodes, means for controlling the potential difference on the electrodes, means for determining when the potential difference falls below a predetermined threshold voltage, to indicate the detection of a sample, means for application a predetermined constant voltage to the sample in response to the detection of the sample, means for measuring the resulting electrical response and means for calculating the concentration of the analyte using the measured electrical response. 8. Измерительный прибор по п. 7, в котором средством для измерения результирующего электрического отклика является амперметр. 8. The measuring instrument according to claim 7, wherein the means for measuring the resulting electrical response is an ammeter. 9. Измерительный прибор по п. 7, в котором средством для измерения результирующего электрического отклика является кулонометр. 9. The measuring device according to claim 7, in which the means for measuring the resulting electrical response is a coulometer. 10. Измерительный прибор по п. 7, дополнительно содержащий средство для приложения второго заранее определенного напряжения к пробе и средство для измерения второго результирующего электрического отклика. 10. The measuring device according to claim 7, further comprising means for applying a second predetermined voltage to the sample and means for measuring a second resulting electrical response. 11. Измерительный прибор по п. 10, в котором средством для измерения второго результирующего электрического отклика является амперметр. 11. The measuring device according to p. 10, in which the means for measuring the second resulting electrical response is an ammeter. 12. Измерительный прибор по п. 10, в котором средством для измерения второго результирующего электрического отклика является кулонометр. 12. The measuring device according to p. 10, in which the means for measuring the second resulting electrical response is a coulometer. 13. Способ измерения концентрации анализируемого вещества в пробе биологической жидкости, нанесенной на полоску для электрохимической диагностики, включающую в себя наложенные друг на друга рабочий и контрольный электроды, при котором подводят заранее определенный постоянный ток источника между рабочим и контрольным электродами, контролируют разность потенциалов на электродах, наносят пробу на полоску, определяют время обнаружения пробы, отмечая, когда разность потенциалов падает ниже заранее определенного порогового напряжения, прикладывают заранее определенное постоянное напряжение к пробе, прикладывают второе заранее определенное напряжение к пробе после первого заранее определенного времени, измеряют электрический отклик в заранее определенное время после первого заранее определенного времени и вычисляют концентрацию анализируемого вещества с помощью измеренного электрического отклика. 13. A method for measuring the concentration of an analyte in a sample of a biological fluid deposited on a strip for electrochemical diagnostics, including working and control electrodes superimposed on each other, in which a predetermined constant current of the source between the working and control electrodes is supplied, and the potential difference on the electrodes is controlled , apply the sample to the strip, determine the detection time of the sample, noting when the potential difference falls below a predetermined threshold voltage, pr imposes a predetermined constant voltage to the sample, applying a second predetermined voltage to the sample after a first predetermined time measured electrical response at a predetermined time after the first predetermined time and calculating the analyte concentration using the measured electrical response. 14. Способ по п. 13, в котором измеряемым электрическим откликом является скорость затухания тока, протекающего через пробу. 14. The method of claim 13, wherein the measured electrical response is the attenuation rate of the current flowing through the sample. 15. Способ по п. 13, в котором измеряемым электрическим откликом является заряд, проходящий через пробу в течение заранее определенного интервала времени после приложения второго заранее определенного напряжения. 15. The method according to p. 13, in which the measured electrical response is a charge passing through the sample for a predetermined time interval after the application of the second predetermined voltage.
RU2000115708A 1999-06-15 2000-06-14 Method for measuring concentration of analyzed substance (variants), measuring device for doing the same RU2238548C2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/333,793 US6193873B1 (en) 1999-06-15 1999-06-15 Sample detection to initiate timing of an electrochemical assay
US09/333793 1999-06-15

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RU2000115708A true RU2000115708A (en) 2002-05-27
RU2238548C2 RU2238548C2 (en) 2004-10-20

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US (1) US6193873B1 (en)
EP (1) EP1067384B1 (en)
JP (1) JP4573400B2 (en)
KR (1) KR100712380B1 (en)
CN (1) CN1160563C (en)
AR (1) AR024351A1 (en)
AT (1) ATE256869T1 (en)
AU (1) AU761438B2 (en)
BR (1) BRPI0002963B8 (en)
CA (1) CA2311431C (en)
DE (1) DE60007229T2 (en)
DK (1) DK1067384T3 (en)
ES (1) ES2212969T3 (en)
HK (2) HK1032820A1 (en)
HU (1) HUP0002275A3 (en)
ID (1) ID26387A (en)
IL (1) IL136700A (en)
MY (1) MY126044A (en)
NO (1) NO20003045L (en)
PT (1) PT1067384E (en)
RU (1) RU2238548C2 (en)
SG (1) SG80681A1 (en)
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