RU1824115C - Method of multiplication of viccinium vitis-idaea l in vitro - Google Patents

Abstract

Use in agriculture and biotechnology, in particular, to obtain lingonberry planting stock. SUMMARY OF THE INVENTION Lingonberry explants are cultured on Anderson's agar medium containing 100 mg / l mesoinositol, 80 mg / l adenine sulfate, 0.4 mg / l thiamine, 0.4 mg / indolylacetic acid. 15.0 mg / l of isopentenyladenine, 30 g / l of sucrose and 7 g / l of agar. It is cultivated on this medium until callus tissue is formed and subsequent shoots are regenerated from it, which are transplanted for rooting on a substrate, which is a mixture of peat and perlite in a 1: 1 ratio, respectively.

Description

The invention relates to a technology for clonal micropropagation of plants. Scope: agricultural and forestry, floriculture, food and medical industry.

The aim of the invention is to develop a new method for the propagation of lingonberry ordinary in vitro culture.

This is achieved by the proliferation of callus and the subsequent regeneration of plants from it on Anderson's nutrient medium containing the following additives: mesoinositol 100 mg / l adenine sulfate 80 mg / l, thiamine 0.4 mg / l, indilyl acetic acid 4 mg / l, isopentenyladenine 15 mg / l, sucrose 30 g / l, agar 7 g / l. Rooting of shoots is carried out on a substrate consisting of a mixture of peat and perlite in a ratio of 1: 1,

In order to obtain calpus, lingonberry seeds were used. Sterilization of their pronp / 1pi n / solution of Ca hypochlorite followed by washing in three shifts of sterile water for 15 minutes each. Then the seeds were planted on Anderson's solid agar medium and cultivated at a temperature of 26 ° C, relative humidity 56%, photoperiod 16h .

After 6 weeks of cultivation, seedlings were removed from the flasks under sterile conditions, divided into explants and planted on the same medium as the seeds. After 6 weeks, the explants formed a callus with subsequent regeneration of plants from it.

Claims (1)

SUMMARY OF THE INVENTION A method for propagating Vaccinium vitls-Idaea L. in vitro, the method comprising culturing explants on an agarized nutrient medium to obtain regenerated plants. that explants are cultured on agar Yo 00 IK Јь 01 Anderson’s medium containing in this cultivation is carried out until at a rate of 100 mg / l of mesoinositis. 80 mg / l of callus and regenerate sulfate adenine proliferation, 0.4 mg / l of thiamine. 0.4 mg / l Indo-tion of shoots from it, the rooting of which lilacetic acid. 15 mg / L isopentenium is carried out from a substrate consisting of a mixture ladenina, 30 g / l sucrose and 7 g / l agar, with 5 peat and perlite in a ratio of 1 1