RU1822785C - Method of preparing of velvet antlers deer extract - Google Patents

Abstract

The invention relates to medicine and the medical industry, and can be used to produce an extract from antler reindeer. The purpose of the invention is the intensification of the method and increase the specific activity of the target product. This goal is achieved by the fact that reindeer antlers of the 2nd and 3rd grade with a fur cover are used as raw materials, the raw materials are crushed to a powder state, extraction is carried out with 50% ethyl alcohol at room temperature, the extracts are acidified to pH 4.7-4.9, then they stand in the cold at minus 20-30 ° C for 12-24 hours, followed by separation of the precipitate. The proposed method allows to reduce the process time to 4-5 days.

Description

The invention relates to pharmaceutical production, mainly to the production of dosage forms from antlers of red and reindeer, elk, saiga and other animals. In particular, it can be used to produce the Rantarin preparation (auth. St. No. 350840) from antler reindeer, which is not inferior in its effectiveness to the well-known Pantocrin preparation.

The purpose of the invention is to increase the biological activity of an extract from antlers while intensifying its production and using antlers of the 2nd, 3rd and even 4th grades.

Using the proposed method in comparison with the prototype provides the following positive effect:

-increases.-yield of biologically active substances by 1.8-3.4 times, even when using antlers of the 2nd and 3rd orth and. as a result, the specific activity of the target product;

- causes intensification of the production of the extract by eliminating the time-consuming manual operation - cleaning antlers from the skin; reducing production time to 4-5 days; facilitating the separation of the clarified extract from the precipitate after freezing due to the acidification of the extract only before freezing, freezing at temperatures below minus 20-30 ° C and using a centrifugation method instead of filtration.

The aim of the invention is achieved in that the difference from the known method for producing a medicinal extract from antlers of antlers

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low grades are crushed to a fine powder with a fur cover; extraction is carried out three times at a ratio of antler weight: extractor -3: 10 for a total time of 3 days; The pH and alcohol content of the extract were adjusted before freezing. Self-freezing is carried out at a temperature of -20 ° C and below. Separation of the extract from suspension particles and colloidal particles of lipoproteins is carried out by centrifugation under cooling.

An example of a specific implementation. The invention is illustrated by the following example. Dried (canned) antlers, together with a fur cover, were crushed on a crusher. then in a Perplex type mill to a fine powder. The use of skin significantly increases the yield of extractives in relative mass, including 3 times less for unsaturated lipids, 2 times for prostaglandins, and 1.8-3.4 times for free amino acids. Then the antler meal was poured into the tincture - mixer and poured with 50% ethanol in a ratio of 3 parts by weight. powder — 10 parts of ethanol without acetic acid, which reduced the extraction of saturated lipids and the excessive extraction of pigments, which hindered the further separation of lipoproteins during freezing. The extraction was carried out with periodic stirring at room temperature for 24 hours. Extraction is repeated three times. After each extraction, the extractor was drained and the same amount of ethanol was poured again. At the end of the extraction, all three drains were combined. The resulting extract was adjusted to the required volume in a ratio of 1:10, the ethanol content to 50%. pH was adjusted to acetic

acid to a value of 4.7-4.9. Then the extract was frozen at -25 ° C for 24 hours, and at -30 ° C and below for 12-15 hours. The frozen extract was clarified by

centrifugation on a preparative centrifuge at 3000-4000 rpm, at 2-4 ° C for 30 minutes. The pH was adjusted to 5.4-5.8 with a solution of sodium alkali, the ethanol content was checked again and, if necessary,

brought it to 50 ± 2%. Chemical analysis was performed for the content of proteins, lipids, phosphates, free amino acids, heavy metal ions and iron, dry residue and ash; biological analysis by

hypo-hypertensive test in rabbits, and also tested the toxicity of the drug in laboratory mice in accordance with the requirements of the provisional pharmacopoeial article N: 42 Extract from antlers of the north

deer (Epsorin) from 10.27.91. In addition, the gamma and beta radioactive activity of the preparation was checked, which should not differ from the background in air.

Claims (1)

SUMMARY OF THE INVENTION A method for producing an extract from antler of deer, comprising grinding the feedstock, triple extraction with 50% ethyl alcohol at room temperature, mixing, combining the extracts, incubating in the cold, separating the precipitate, characterized in that, in order to intensify the method and increase the specific activity of the target product, reindeer antlers of the second and third grades with a fur cover are used as raw materials, the raw materials are crushed to a powder state, before incubation, the extract under acid to pH 4.7-4.9, and incubated at -20 ... 30 ° C for 12-24 hours.