RU1818048C - Method of purification of protein-containing raw acidic hydrolysate - Google Patents

Abstract

The invention relates to biotechnology and relates to a method for the purification of an acid hydrolyzate of a protein-containing feed. Ca (OH) g is introduced into the hydrolyzate to a pH of 3.5–4.5, mechanical impurities are separated off, boats are separated by sulfuric acid to a pH of 1.7–2.0, passed through a cation exchange column and amino acids and peptides elute with cation exchange resin: 2-3% NaOH solution taken in a volume equal to the volume of cation exchange resin. 1 s.p. f-ly.

Description

The invention relates to the microbiological industry, in particular to a method for the purification of an acid hydrolyzate used as a nitrogen base in culture media for the cultivation of microorganisms.

The aim of the invention is to simplify and improve quality by reducing the contaminants of the target product in the eluate.

This is achieved by the fact that in the method of purification of an acid hydrolyzate, including the addition of calcium hydroxide, the separation of mechanical suspensions, and the separation of amino acids and peptides with a cation exchange resin, sulfuric acid is added to the pH of 1.7-2.0 with a cation exchange resin before the separation of amino acids and peptides isatu. previously purified from mechanical suspensions by filtration after adding calcium hydroxide to pH 3.5-4.5; moreover, the elution of amino acids and peptides is carried out with a 0.5-0.8 N sodium hydroxide solution.

: The essence of the method is that the introduction of calcium hydroxide into the hydrolyzate to a pH of 3.5-4.5 before separating the mechanical suspension and its acidification with sulfuric acid to a pH of 1.7-2.0 before applying the hydrolyte to cation exchange resin reduces the amount of accumulating in cation exchanger pollutants, and also facilitates the elution of amino acids and peptides with an alkaline solution. The proposed complex of fairly simple techniques allows lower; to contaminate the content of polluting substances in the eluate to a level that ensures the production of a nitrogen base simple neutron. implementation, without additional complex methods of clarification and tertiary treatment of eluct.

The proposed method for the purification of an acid hydrolyzate is implemented as follows .-; ,. PRI me R 1. In the sulfuric acid hydrolyzate of protein-vitamin concentrate before separation of mechanical suspensions introduced a solution of calcium hydroxide to pH

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3.5, after which the hydrolyzate was filtered. This; Г 1Др6лй exposure contained 950 mg% total nitrogen, 365 mg% amino nitrogen, including 320 mg% amino nitrogen amino acid, 2000 mg% carbohydrates and 366 mg of their degradation products. Before serving

hydrolyzate to cation exchange resin was added sulfuric acid, lowering the pH to 1.7. After that, 1 liter of hydrolyzate was applied to the cation exchange resin, with a volume of 1 liter, at a space velocity referred to the free section of the cationite chamber (M3 / s) / m2 (hereinafter referred to as speed). Then the cation exchange resin was washed with water at a speed of 2 m / s. Elution was performed with 1 L of a 0.5 N sodium hydroxide solution at a rate of 5 TO 4 m / s, while taking 1 L of the eluate. This eluate contained 505 mg% of total nitrogen, 326 mg% of amine nitrogen, c. including 315 mg% of amino nitrogen of amino acids, 34 mg% of carbohydrates and 16 mg% of products of their 0 degradation and 0.55% sodium hydroxide.

: -; G ;; From this ethanol eluate, a nitrogenous base for microbiological purposes was obtained by neutralizing it with hydrochloric acid. At the same time, the salt content in the dry residue of such a base did not exceed 20% and for most nutrient media, entry into their composition will not exceed the limits of its permissible contents in these

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PRI me R 2. According to example 1, a solution of calcium hydroxide increased the pH to 4.5; then, the pH was reduced to sulfuric acid to 2 DC. A 0.8 N sodium hydroxide solution was eluted at a rate of m / s, while taking 1 liter of eluate containing 497 mg% total nitrogen, 317 mg% amine nitrogen, including 311 mg% amine nitrogen

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amino acids, 38 mg% carbohydrates and 18 mg% products of their destruction and 0.6% sodium hydroxide.

After elution, the cation exchange resin was washed with water and regenerated with a 3-4% solution of sulfuric acid. Moreover, after using cation exchanger 20 times, the quality of the resulting eluate remained practically unchanged.

In the eluate, in comparison with the initial hydrolyzate, the content of carbohydrates and products, their destruction is reduced by more than 10 times, and the amino acid content of amino acids is increased by 1.6 times.

Losses of amino nitrogen of amino acids were minimized and amounted to no more than 3%.

The proposed method for the purification of an acid hydrolyzate will find wide application in the creation of nitrogenous bases for microbiological purposes.

Formulas of the invention

Claims (2)

1. A method for purifying an acid hydrolyzate of a protein-containing raw material, comprising separating mechanical impurities, passing through a cation exchange resin column, eluting amino acids and peptides from the cation exchange resin with sodium hydroxide solution, characterized in that, in order to simplify the process and improve the quality of the target product, into the hydrolyzate before mechanical removal calcium hydroxide is introduced into the impurities to a pH of 3.5-4.5; after separation of the mechanical impurities, the hydrolyzate is acidified with sulfuric acid to a pH of 1.7-2.0. 2. The method according to claim 1, wherein the elution is carried out with a 2-3% sodium hydroxide solution, taken in a volume equal to the volume of cation exchanger.