RU1792598C - Method for aquarium fish selection - Google Patents

Description

| The invention relates to fishing

namely, the selection of such industrial breeding objects as tilpium and find application in the selection of other

ides of fish, the peculiarity of which is gestation in the mouth.

I There are known methods for increasing the variability of whitefish and cyprinids, which include processing of fertilized eggs with supermutagens and their subsequent incubation in crystallizers or incubation apparatus. However, these methods are unacceptable for working with NLPs, since during incubation of eggs of calf in the non-rotational cavity of fish, it completely dies on the second day. In the oral cavity of Tilpium, due to secretory activity), then the optimal conditions are created for the incubation of eggs, providing 100% survival of the embryos. On this, the morphogenetic effect of supermutagens is more pronounced, since it is screened to a much lesser extent by the influence of external environmental factors than during incubation of caviar of cyprinids and whitefish in incubation apparatus or in crystallizers.

The purpose of the invention is to obtain breeding material with increased phenotypic and genotypic variability and to increase the efficiency of measures for breeding specialized, highly productive forms of fish.

An increase in the efficiency of breeding processes is associated with an expansion of hereditary variability in offspring, which is provided by a specific action on the stands of super mutagens capable of inducing a wide range of genetic changes (mutations) affecting the biochemical, physiological, and morphological characteristics of fish.

A specific feature of the use of chemical supermutagens on teal pi x is

w

Yo

Vj

Yu

S3

ate s

The fact that, together with caviar, enters the oral cavity of fish, supermutagens are able to penetrate into the gonads and affect the young germ cells of fish of the next generation, which are more sensitive to mutagens than fertilized eggs. This explains a significant increase in phenrtypic variability in the offspring of tyli pi following the offspring obtained from eggs directly treated with supermutagens (see table) -; :; -;.;, - -; ::,: ..: - - V ...- ..

The proposed method is carried out as follows.

Tillpium producers selected for breeding are screened or fenced off from the bulk of the fish and are incited to spawn by using pituitary injections. After a certain time after the female (in some species the male) collects the fertilized eggs in the oral cavity, the fish is caught, the eggs are removed from her oral cavity and the eggs are placed in a crystallizer with a certain amount of water. Then, the mother liquor of supermutagen, which is prepared in plain water, is poured into the crystallizer with caviar.

The exposure of the treatment is 120 minutes, after which the treated eggs are transferred to the container where the female (male) is located, which immediately takes it back into the oral cavity and carries it until fry appear.

When the hatched fry are 3-4 days old, they are planted for cultivation, and the female (pre-labeled) is planted for feeding under conditions of good food availability.

After 20-25 days of feeding for this female, a male is selected and repeated spawning is organized. With repeated spawning, offspring are obtained in the usual way, without resorting to mutagenic processing of eggs.

Both of the resulting offspring are subsequently used as starting material for selection.

The temporary regime of mutagenic treatment of fish roe, equal to 120 minutes due to the fact that it ensures the complete penetration of the mutagen into the eggs and at the same time does not entail negative consequences for the developing calves of pi.

A high semi-lethal concentration of super mutagens is taken so that part of the mutagen can reach the producer's developing germ cells and provide increased genetic variation in the next offspring.

Example 1. Caviar of fish, for example, Mozambique tilium, is treated with nitrosoethylurea (NEM) in a semi-lethal concentration. For this, 1 part of a 1% mother liquor NEM is poured into 500 parts of ordinary water in which the processed caviar is located.

Processing exposure 120 min. Then, the treated caviar is returned to the female, who immediately takes it to the oral cavity. Subsequently, the procurement and rearing of offspring is carried out according to a conventional technique.

The spawning female is walked in

5 for 20-25 days and then used again to produce offspring without the direct use of mutagens.

Phenotypic variability of breeding traits in offspring obtained

0 from eggs treated with supermutagen, (see table prtomstvo-2) increased by 2.12-33.4% and in offspring from repeated spawning (table progeny-3) by 13.7-64.8%.

PRI me R 2. Caviar of fish, for example,

5 Mozambique tilpium is treated with ethyleneimine (AI) in semi-lethal concentrations. For this 1 hour, a 1% mother liquor HEY is added to 750 hours, the ordinary water in which the treated caviar is located:

Processing exposure 120 min. The treated eggs are then returned to the female, which takes her into the oral cavity. Subsequently, the production and cultivation of the seed is carried out according to the generally accepted technique.

The spawning female is fed for 20-25 days and then used again to produce offspring without the immediate use of mutagens.

The phenotypic variability of breeding traits in offspring obtained from eggs treated with supermutagen (in the offspring-2 table) increased by .1,155 85.7% and in offspring from repeated spawning (in the offspring-3 table) by 22.7-87.5%.

The proposed method makes it possible to expand the possibilities of breeding with til pi, a new object of industrial fish farming in the USSR. This is all the more important because tilli are poorly adapted to the conditions of industrial fish breeding farms in our country, which are characterized by extreme instability. On the other hand

55, the method can find effective application in breeding aquarium objects, which include some species of tilpium. .

Claims (1)

Formula from acquisition A method of breeding tilpium involving selection of producers, procreation and subsequent crossbreeding, characterized in that, in order to obtain higher heteroeterogenic offspring and increase the efficiency of breeding processes, procreation is carried out in two stages, the first of which is selected from selected producers From the oral cavity, fertilized eggs are treated with supermutagens and returned to producers for incubation and juvenile production; in the second stage, repeated spawning is carried out Odita; oil, and eggs are incubated without its removal from the oral cavity, and is used for mating the progeny obtained from step I and II. Variability of economically valuable traits of Tilpium fry after 30 days of cultivation in experiments using super mutagenes