RU1792270C - Method of rooting nut-fruited crop shoots obtained in vitro - Google Patents

Abstract

Usage: in agriculture and biotechnology, in particular, when receiving planting material of nut-bearing fruit in a tissue culture. The essence of the invention: rooting of shoots is carried out on a nutrient medium Murashige-Skoog, containing in its composition macrocells with four-fold dilution, 60 g / l of sucrose. Cultivation was carried out in the dark for 5-7 days, after which they were transplanted onto a Murasige-skug nutrient medium containing four-fold dilution macrocells, 30 g / l sucrose and an additional 7 g / l agar. Cultivation on this medium is carried out at a sixteen-hour photoperiod. 2 tab.

Description

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The invention relates to biotechnology, in particular to a method of cultivating plants in vitro, and can be used in forestry, nursery.

... It is known that auxin containing nutrient media are used to root the Lactiferous shoots obtained in tissue culture, or the basal end of the shoots is treated with hormones before they are planted directly on the substrate.

A two-stage rooting of the shoots is also proposed, in which, at the first stage, root formation in the dark is induced in the presence of auxins (IMC). The appearance of roots is observed on the same medium, but with the addition of activated charcoal or with a decrease in auxins.

Closest to the proposed solution is a method of rooting shoots of hazel obtained in vitro, in which the shoots were placed base in

liquid culture medium K (h), consisting of half the concentration of Cheng mineral salts with IMA (10 mg / l). and kept at a temperature of 25 ± 2 ° C with a phototerride of 18 hours. After 5 days, the shoots were transferred to fresh liquid medium of the same composition, but without BCI.

A disadvantage of the prototype is that this method is used for monoculture.

The aim of the invention is the expansion of the number of rooted species of walnut, propagated by the microclonal method, for forestry and nursery. . ; ... ....

This is achieved by rooting the walnut in two stages: first on a liquid nutrient medium with auxins (10 mg / l), at a temperature of 25 ± 3 ° C, and then on a hormone-free medium of the same composition.

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Unlike the prototype in the proposed method, the rooting of walnut shoots is kept for 5 to 7 days in the dark on a liquid nutrient medium Murashige-Skoog, microelements of which are diluted 4 times, the amount of sucrose is increased to 60 g / l, and then transferred to this same medium, but already with 7 g / l agar. The amount of sucrose is reduced to 30 g / l. Shoots are cultivated with a 16-hour photoperiod.

Example p. The shoots obtained by microclonal propagation of walnut and reached a height of 18-20 mm are placed with basal ends on filter paper bridges in sugar jars with Murashige-Skoog liquid nutrient medium of the composition shown in Table. 1, and cultivated in tomton at 25 ± 3 ° C. After 1, 3, 5, 7, or 10 days, the shoots are transferred to a nutrient medium of the same composition, but without BCI, the amount of sucrose is reduced to 30 g / l, 7 g / l of agar is added and exposed to a 16-hour photoperiod. The shoots are kept on this medium until the roots appear ..

The control is walnut shoots, which are grown for 5 or 7 days in the dark on a liquid nutrient medium Murashige-Skoog with a full set of macronutrients, 60 g / l sucrose and 10 mg / l IMC. After 5 or 7 days, the shoots are transferred to a 16-hour photoperiod on a nutrient medium of the same composition, but without BCI, with the amount of sucrose - 30 g / l and 7 g / l agar (Table 1).

As can be seen from the table. 2, with 5-day aging of shoots in a liquid medium with

. The composition of the nutrient media used in rooting walnut shoots

in vitro. -

IMA in 9.0% of shoots roots appeared after a week of cultivation on a hormone-free medium. Subsequently, the number of rooted shoots increases and after 4 weeks reaches 72.0%. With a 7-day exposure with BCI, 6.0% of rooted shoots are obtained by the end of the eighth week. In the control variants, the roots did not appear.

Thus, the rooting of walnut shoots obtained in an in vitro culture was carried out.

The claimed technical solution allows to expand the object of plants rooted in vitro.

Claims (1)

SUMMARY OF THE INVENTION A method for rooting nut shoots obtained in vitro, including cultivating shoots at a temperature of 25 ± 2 ° C in a liquid nutrient medium containing macronutrients, indolylbutyric acid in an amount of 10 mg / l and sucrose, followed by transplantation to a hormone-free nutrient medium and growing to root formation and obtaining regenerated plants, characterized in that the shoots are cultivated for 5-7 days, in the dark on a Murashige-Skoog medium, with four-fold dilution of the macroelement comrade, containing 60 g / l sucrose, subsequent change to the culture medium of Murashige-Skoog medium with a fourfold dilution macrocells containing 30 g / l sucrose and an additional 7 g / l agar, and cultivation on this medium is carried out at a sixteen-hour photoperiod. Table 1 The number of rooted shoots of walnut on a nutrient medium Murashige-Skoog at a dilution of 1: 4 macrocells and with the addition of IMC (Yumg / l) Continuation of table 1 table 2