RU1787437C - Agent for prophylaxis and treatment fishes with aeromonosis - Google Patents

Abstract

The invention relates to the field of veterinary medicine, in particular to means for controlling aeromonosis. As an agent for the prophylaxis and treatment of fish during aeromonosis, the drug Polymyxin M-sulfate was used. Polymyxin M-sulfate for disease prevention is given with feed at a concentration of 0.002-0.003% (based on 0.2-0.3 g per 10 kg of feed), and in the treatment of 0.005-0.006%, the concentration of polymyxin (0.5-0.6 g per 10 kg of feed). Feed the animals daily for 7 days.

Description

The invention relates to veterinary ichthyopathology, in particular to the development of agents for the prophylaxis and treatment of fish in aeromonosis.

The purpose of the invention is to provide an agent having high prophylactic and therapeutic effects in acute septic disease of cyprinids, aeromonose, which does not cumulate in organs and tissues.

This is achieved through the use of the drug, polymyxin M-sulfate. Total formula: CsiHgeSieOu. According to the typical composition of amino acids (found a, y-aminobutyric acid, threonine, leucine, etc.) is close to polymyxin A.

When administered orally, the drug is rapidly absorbed and, without undergoing biochemical transformations, is completely excreted from the body within 4-5 days.

New functional use of the drug, previously used in medicine and veterinary medicine for gastrointestinal diseases, allows us to identify a new

the property is to prevent and treat an object that is completely different from warm-blooded, related to cold-blooded in case of disease unusual for warm-blooded and specific for cyprinids with septic process -aeromonosis. .Example 1. Put 3 aquariums, a capacity of 500 l, each planted 25 specimens of two-year-old carp. Fishes were infected intraperitoneally, depending on the weight of 0.2-0.3 ml 24 hours by broth culture of mixed 5 pathogenic strains of aeromonads. .....

In one aquarium, the prophylactic effect of polymyxin M-sulfate was tested at a concentration of 0.002% (0.2 g per 10 kg of feed), in another, for comparison, a 0.04% concentration of furazolidone (4 g per 10 kg of feed). The preparations were mixed with mixed feed and given 4-5 hours after infection for 7-10 days, respectively. In the third control aquarium, infected fish were given food without drugs.

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The observation period for the experimental fish is 21 days after the last feeding of the preparations.

As a result, in two aquariums in which fish were fed with the addition of 0.002% polymyxin and 0.04% furazolidone, not one fish became ill, however, according to the preparations, on the 15th and 16th day of the last feeding, they became ill with aeromonosis (with a different clinic) 5 and 4 fish, respectively, which amounted to 16%. In the control aquarium, out of 125, 22 fish specimens died within 2-6 days, which is 88%.

Example 2. Put 2 aquariums with a capacity of 500 l, each planted 25 specimens of two-year-old carp. Fishes were infected 24 hours with a broth culture of pathogenic strains of aeromonas intraperitoneally at doses of 0.2-0.3 mm.

In one aquarium, a 0.003% concentration of polymyxin (0.3 g: 10 kg feed) was tested, another was taken for control, in which the fish were fed without preparations. For comparison with furazolidone (prototype), the results of Example 1 were taken into account.

As a result, in the experimental aquarium, where the fish were given food with polymyxin, not a single fish fell ill during the entire observation period (21 days), while when feeding with furazolidone (from Example 1), aeromonosis 4 fatal. In a control aquarium, out of 25 fish fell ill with aeromonosis for 2-6 days, 21, which is 84%. Preventive doses (0.002 and 0.003% concentrations) were harmless to the fish. The drug is completely excreted on the 7-8th day, however, a 0.003% concentration is effective for a longer time than 0.002%.

Thus, when comparing polymyxin with furazolidone, it turned out that polymyxin, despite relatively low doses, has a higher prophylactic effect ..

Example 3, We put 3 aquariums with a capacity of 500 l, each planted 25 specimens in two-year-old carp. Fishes were infected intraperitoneally for 24 hours with a broth culture of aeromonad in doses of 0.2-0.3 ml.

A concentration of polymyxin M-sulfate (0.5: 10 kg feed) was tested in one aquarium, and 0.06% furazolidone (6.0 g, -10 kg feed according to the instructions for the control of carp aero mononomy) was compared in another for comparison. The preparations were mixed with compound feed and given daily after the first clinical signs (on the 4th day) of the disease appeared for 7 days with the use of polymyxin and

10 days - furazolidone. In the third control aquarium, fish were given food without preparations.

As a result, in two aquariums in which fish were given food with preparations, the whole fish recovered, but in the aquarium where furazolidone was used, exacerbation of the disease began on the 18th day after the drug was stopped, which is 16%, which is 16%.

Example 4. In an aquarium with a capacity of 500 l, 25 two-year-old carp were planted, which were infected for 24 hours with a broth culture of pathogenic strains of aeromonads, in doses of 0.2-0.3 ml.

3 days after infection (after the clinical signs of the disease appeared in most infected fish), a feed containing 0.006% of the preparation (0.6 g: 10 kg of feed) was given daily for 7 days. The furazolidone and control data are taken from Example 3.

As a result, when using feed containing 0.006% polymyxin for 7 days, the whole fish recovered, and during the entire observation period (21 days), the disease did not exacerbate.

When comparing two drugs: polymyxin at concentrations of 0.005-0.006% and furazolidone 0.06%, it was noted that polymyxin M-sulfate had the best therapeutic effect and was used for 7 days, with no difference in the effects of both concentrations .

Thus, the use of polymyxin for the prophylaxis and treatment of fish with aeromonase is obvious since it provides the 1000th efficiency.

Economic efficiency when using polymyxin consists of: 100% therapeutic and prophylactic effect of the drug (with timely use), lower dose (0.005-0.006%) compared with furazolidone (0.06%), duration of use (7 and 10 days, respectively) ), longer action (21 and 18 days, respectively) and there are no complications when using polymyxin.

As a result, despite the fact that the cost of polymyxin is 245 p. 85 K., and furazolidone 33 p. due to all these indicators, the economic effect is obvious,

The use of the drug polymyxin in diseases of fish, mainly cyprinids, will find wide application in fish farming.

Claims (1)

The claims The use of a polymyxin M-sulfate preparation as a means for the prevention and treatment of fish in aeromonosis.