RU1779691C - Disintegration of microorganisms - Google Patents

Abstract

Usage: in biotechnology and the microbiological industry in the processes of disintegration of microorganisms. SUMMARY OF THE INVENTION: The disintegrator comprises a cylindrical working chamber, a drive shaft with a stirrer located therein, and bundles of elastic wires serving as working elements for influencing the cells of microorganisms. Bundles of wires are located along the entire length and circumference of the drive shaft or in the form of other disks on it. On the wall of the chamber are also placed bundles of wires in the form of rings. The axis of the wires on the shaft can be perpendicular to the axis of the shaft in cross section or at an angle to it. 4 ill.

Description

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The invention relates to biotechnology and can be used in biotechnology, medical and microbiological industries.

A known disintegrator of microorganisms containing a chamber with nozzles filled with grinding media and a mouth, an agitator (ed. St. No. 745940, class C 12 K 1/10, 1980)

A disadvantage of the disintegrator is the contamination of the disintegrants with the material of grinding media in the case of using polymer grinding media. When the disintegrator operates with glass grinding media, the disintegrator is contaminated not only with colloidal glass particles, but also with colloidal metal particles (Ni, Cr, Fe, etc.), of which the chamber and rotor are made. Cleaning disintegrators requires a significant investment of time and money. The biochemical activity of disintegrators from interaction with metal ions is reduced.

Known disintegrator of microorganisms containing a chamber with grinding media and a stirrer.

Its disadvantages are the same as that of the previous disintegrator. These are the disadvantages of all ballistic disintegrators, which reduce the quality of disintegrators.

The purpose of the invention is to improve the quality of disintegration.

This goal is achieved by the fact that in a known disintegrator containing a chamber with technological nozzles and a drive shaft with a stirrer and bundles of elastic wires serving as working elements for acting on cells, bundles of wires are located along the entire length and circumference of the drive shaft or in the form of separate disks on it, while on the wall of the chamber also

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bunches of fibers are placed in the form of rings between these disks.

When the stirrer rotates, the rigid and elastic wires of the stirrer and rings, oscillating with high frequency up to ultrasound, create gradient microcurrents between themselves, providing a high-intensity fluid shift, which leads to the destruction of microorganisms.

In FIG. 1 schematically shows a disintegrator, section; in Fig.2 - the same, with ring wire ribs; in FIG. 3 and

4- the arrangement of wires on the stirrer is perpendicular or inclined to the shaft, respectively.

The disintegrator comprises a chamber 1 with an inlet pipe 2 and an outlet 3, a shaft 4, on which elastic wires are fixed

5 and 6 located around the axis of the shaft perpendicular to the axis or at an angle to it.

A different type of wire stirrer is necessary for the disintegration of various types of microorganisms and the creation of different shear stresses,

The design of the mixer can be made in the form of a set of wires in the form of separate disks 7, between which bundles of wires 8 in the form of rings are placed on the wall of the chamber.

The disintegrator works as follows.

The suspension of microorganisms is supplied into the chamber 1 through the nozzle 2. Interaction with the high speed moving wires 5 on the rotating shaft 4, the cells of the microorganisms are destroyed and the disintegrator leaves through the nozzle 3.

The disintegrator is simple in design due to the absence of grinding media and, as a consequence, the exclusion of separators - devices that delay grinding media in the chamber. The quality of the disintegrator is improved due to the absence of grinding. The cost of extracting biochemical compounds from the disintegrator is reduced by eliminating its purification from salting.

Claims (2)

1. A microorganism disintegrator, comprising a cylindrical working chamber with technological nozzles, a drive shaft with a stirrer, and bundles of elastic wires serving as working elements for affecting microorganism cells, characterized in that, in order to increase the uniformity of the disintegrator in terms of dispersion and thereby improve quality of the final product, wire bundles are located along the entire length and circumference of the drive shaft or in the form of separate disks on it, while bundles of dragging in the form of rings between these disks. 2. The disintegrator according to claim 1, with the fact that the axis of the wires on the shaft is perpendicular to the axis of the shaft in cross section or at an angle to the shaft. (Pt / e.f fig.Z FIG. 2. fa g a