RU1427644C - Antibacterial complex for sanitation of agricultural animal sperm - Google Patents

Abstract

The invention relates to agriculture, in particular to the artificial insemination of farm animals. The aim of the invention is to reduce the bacterial contamination of diluted semen, increase its shelf life and increase the fertility of the uterus. 10-50 mg of gentamicin, 100000-300000 PIECES of penicillin and 0.1-0.3 g of streptocide are added to the medium prepared according to the instructions and cooled to kO ° C; As a result of the use of this complex, the microbial load of diluted semen is reduced by a factor of 4.6 compared with spermosan, fertility is increased by 7.66, and multiple pregnancy in pigs by 0.21 pigs. 4 tab.

Description

cl

with

The invention relates to agriculture, in particular to the artificial insemination of farm animals, mainly to the storage medium of sperm of breeders.

The aim of the invention is to reduce the bacterial contamination of diluted semen, increase its shelf life and increase the fertilization of the uterus.

Example 1. The proposed complex is used as follows. 25 mg of gentamicin 250,000 IU of penicillin and 0.3 g of streptocide per 1 liter are added to the HCCC-8 medium prepared according to instructions (7) and cooled to 40 ° C. The prepared sperm was diluted with sperm of the boars according to the instructions.

EXAMPLE 2 Gentamicin was added to the HCCC-8 medium at the rate of 10, 20, 40, 200 and 2000 mg per 1 liter of medium. The same medium with spermosan serves as a control. In the table. Figure 1 shows the effect of various doses of gentomycin on the survival rate of sperm of the pancreas. According to the data presented in table. 1, gentamicin increases sperm survival in a wide range of concentrations - from 10 to 200 mg / l. Sperm survivability is highest when 20 and 40 mg of gentamicin is added per liter of medium. These concentrations of gentamicin increase sperm survival by 13.8, respectively, compared to spermosan.

Therefore, the introduction into the environment of only gentamicin increases

Isd

O5 4 4ib

sperm life is more significant than spermosan administration. In the HCCC-8 medium without antibiotics, sperm survival rate is 11.7 lower than in the medium with gentamicin and 20.1-22.6% less than in the medium with spermosan.

Example 3. In the table. 2 | presents the results of the selection of optimal; BUT the ratio of the components of the new

antibacterial complex. A positive effect (an increase in survival by 28-39% of the control with sperm, mosan) is observed when the complex components are combined in a wide range of their concentrations in HCCC-8 medium: gentamicin 10-50 mg, penicillin 100000-300000 IU, streptocid 0. 1 - 0.3 g per 1 liter of medium. The highest survival rate of sperm cells - 139.6% of control with spermosan - is ensured by the following combination of components per 1 liter of medium: gentamicin - 25 mg, penicillin 250,000 EL, streptocide 0.3 g.

Example 4. A comparative test was conducted of the control antibacterial complex of spermosan-3 (prototype) and a new antibacterial complex containing the optimal amounts of components: gentamycin 25 mg, penicillin 250,000 units, streptocide 0.3 g per 1 liter of medium. The effectiveness of sperm sanitation, fertility and multiple pregnancy of sows inseminated with sperm diluted with GCC-8 medium were studied.

Table 1

The effect of different doses of gentamicin on the survival rate of sperm of hcp in HCCC-8

(n 9)

 BOB - an absolute indicator of sperm survival rate (8)

5

Q

0

5

0

5

the addition of spermosan-3 or a new antibacterial complex. As a result of the use of this complex, a decrease in the microbial contamination of diluted semen by a factor of 6 was found in comparison with spermosan (see Table 3). Fertility of sows (table) when using medium with a new antibacterial complex is 7.66%, and multiple pregnancy is 0.21 piglets higher than in control with spermosan.

Thus, the use of the proposed antibacterial complex provides a reduction in bacterial contamination and an increase in the storage period of sperm, an increase in fertility and multiple sows compared with the use of spermosan-3.

Claims (1)

The claims An antibacterial complex for sperm sanitation of agricultural animals, including penicillin and streptocide, characterized in that, in order to reduce the bacterial contamination of diluted semen, to increase its shelf life and increase the fertility of the uterus, it additionally contains gentamicin in the following ratio of ingredients per 1 liter Wednesday: Gentamicin, mg 10-50 Penicillin, UNIT 100000-300000 Streptocid, g 0.1-0.3 table 2 The effect of different ratios of the components of a new antibacterial complex with gentamicin on sperm survival XP kov {n 19) P..R note: + G- gentaiicin, -m-G-penicillin, -nm-C - streptocide, control I - sperm diluted with HCCC-8 medium with spermosan, control II - semen diluted with HCCC-8 medium without sanitizing additives  TABLE 3 A Efficiency of sanitation of sperm of hryvnia, standard spermosan or antimicrobial complex with genta-: mycin 1: 3 1: 2 1: 3,5 1: 4 1: 3 1: 3 1: 2 1: 2 1: 4 1: 3.5 1: 4 1: 3,5 1: 3 1: 5 1: 3,5 1: 4 1: 4 1: 3 1: 4 1 116 19 1 144 150 32 6 eleven li 2764i  The sperm was diluted with HCCC-8 medium so that one sperm dose had a volume of 125 ml and contained 5 billion active sperm f Table  Fertility of sows inseminated with freshly obtained sperm diluted with HCCC-8 medium with the addition of spermosan or an antibacterial complex with gentamicin 8 Continuation of Table 3