CN105994251B - A kind of dilution preservation liquid for killing animal semen virus and bacteria - Google Patents

A kind of dilution preservation liquid for killing animal semen virus and bacteria Download PDF

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CN105994251B
CN105994251B CN201610439771.XA CN201610439771A CN105994251B CN 105994251 B CN105994251 B CN 105994251B CN 201610439771 A CN201610439771 A CN 201610439771A CN 105994251 B CN105994251 B CN 105994251B
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parts
dilution
virus
liquid
bacteria
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CN105994251A (en
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况洪
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CHENGDU MEIQIANG VETERINARY TECHNICAL SERVICE Co Ltd
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CHENGDU MEIQIANG VETERINARY TECHNICAL SERVICE Co Ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A01N1/0215—Disinfecting agents, e.g. antimicrobials for preserving living parts
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/08—Alkali metal chlorides; Alkaline earth metal chlorides
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/12—Iodine, e.g. iodophors; Compounds thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/10—Animals; Substances produced thereby or obtained therefrom

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  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
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  • Microbiology (AREA)
  • Virology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The present invention provides a kind of dilutions for killing animal semen virus and bacteria to preserve liquid, by weight, including following component：0 0.002 parts of 25 40 parts of glucose, 2.5 3 parts of citric acid, 58 parts of trisodium citrate, 1.2 1.3 parts of sodium bicarbonate, 1.2 1.3 parts of disodium ethylene diamine tetraacetate, 0.7 0.9 parts of potassium chloride, 2.8 3 parts of trishydroxymethylaminomethane, 0.05 0.08 parts of cysteine, 0.025 0.030 parts of povidone iodine and antibacterial peptide.For the dilution of economic animal sperm, preservation and kill bacterial virus.

Description

A kind of dilution preservation liquid for killing animal semen virus and bacteria
Technical field
The invention belongs to farm animals artificial insemination fields, and in particular to a kind of to kill the dilute of animal semen virus and bacteria Release preservation liquid.
Background technology
The advantage of technology of artificial insemination is can be made by certain technological means by manually acquiring the high-quality sperm of animal It can preserve transport for a long time, and great economic benefit is brought for animal husbandry breed improvement and raising production performance.But it is artificial Insemination techniques also have its natural defect, that is, the pathogenic microorganism that may be carried inside donor sperm is all transmitted to its Receptor.By taking pig as an example, 10 are usually contained in boar semen4~105 CFU/ml bacteriums and the unknown virus that may be polluted and other Pathogenic microorganism.These bacteriums and virus can influence the holding time of boar semen and the motility rate of sperm, and can pass through people Boar may be carried pathogenic microorganism and be transmitted to sow and piggy by work insemination techniques, lead to sow breeding difficulty, piggy disease The significantly decline of the prevalence and production performance of disease, causes serious economic loss.
Domestic and foreign scholars' research shows that the main virus for endangering pig production that can be detected in boar semen has：Aftosa Virus（FMDV）, swine fever virus（CSFV）, pig circular ring virus（PCV-2）, porcine pseudorabies virus（PRV）, pig breeding with breathing it is comprehensive Close syndrome virus（PRRSV）, pig parvoviral（PPV）, Japanese B encephalitis virus（JEV）.Wherein have proven to CSFV, PRRSV, PPV, PRV can infect sow by artificial insemination and cause the breeding difficultys such as stillborn foetus, miscarriage.Herd boar sperm band poison is swinery virus One important channel of sick vertical transmission, and restrict a great problem of artificial fertilization technology's development and popularization.
Animal semen dilution preservation liquid is extensive in artificial insemination sector application, and technical difficulty is not high, is formulated simple easy life Production, major function are to increase sperm volume, prevent cold stress, provide sperm nutrition, the buffer solution as high ph-values, extend essence The time-to-live of son.But it is polluted for boar semen, existing technology is by the way that antibiotic is added in preserving liquid in semen dilution （Such as penicillin, streptomysin, gentamicin, lincomycin）It is next antibacterial, but antibiotic is only capable of inhibiting the part in sperm thin Bacterium breeds, and completely ineffective to virus, and has the risk of abuse of antibiotics.Therefore, it is led at present in economic animal artificial insemination There are no better methods in domain to handle bacterium and the viral containment issues in sperm.
Such as application for a patent for invention 201510756292.6, discloses the fresh essence of a boar and preserve long-acting dilution powder and preparation method With application.It is the glucose sugar 35-40 by parts by weight, ethanedioic acid tetraacethyl sodium 1.2-1.4, sodium citrate 4-8, sodium bicarbonate 1.2-1.4, potassium chloride 0.7-0.8, polyvinyl alcohol 0.4-0.6, cysteine 0.1-0.2, inositol 0.4-0.6, penicillin 1.0- 1.5, streptomysin 1.0-1.5 etc. raw materials form, and there are problems that abuse of antibiotics.
Application for a patent for invention 201410164618.1 discloses the formula composition of the antiviral semen diluent of a boar.Base In every 1000 milliliters of double distilled waters, the antiviral semen diluent of pig contains：15.50 the glucose of part；11.65 parts of citric acid three Sodium；2.35 parts of EDETATE SODIUM salt；1.75 parts of sodium bicarbonate；1.00 parts of polyvinyl alcohol；5.50 parts of three carboxymethylamino first Alkane；4.10 parts of citric acid；0.07 part of cysteine；1.00 parts of vitamin C；0.10 part of Enrofloxacin；And 50ml Antivirotic.Containing antiviral ingredients such as Ribavirins in formula, although Ribavirin has certain effect for inhibiting viral growth, But country forbids the antiviral drugs that animal and veterinary industry uses.
The development difficulty for killing the semen dilution preservation liquid of animal semen virus and bacteria is embodied in：
A, safety first otherwise damages sperm motility rate, is not impacted to fertilized eggs, embryo and the breeding of sow, On the heritability of sperm without influence.
B, validity is the key that this technology effectively to harmful cause of disease inside sperm, especially virus.
C, effective protection can be provided to sperm, the sperm storage phase can reach 7-10 days.
D, safety and validity are conflicts, it is difficult to the unity of opposites.
E, study condition requires high, it is desirable that pharmacy, veterinary bacteriology, virology, virocyte culture and herding Etc. relevant specialities and experimental condition, single enterprise or research unit are difficult to meet.
Document " a kind of influence of the sperm purification protective agent to boar sperm motility rate and is made the killing that 5 kinds viral in sperm With research "（Chinese herding magazine, breeding physiology, the 7th phase of volume 51 in 2015）, disclose a kind of sperm and purify protective agent to public affairs The influence of Boar spermatozoa motility rate, and to being present in killing effect viral in boar semen.The sperm of the document purifies protective agent It is not suitable for the disinfectant containing iodine.
Invention content
In order to solve the above technical problem, the present invention provides a kind of dilution preservations for killing animal semen virus and bacteria Liquid.Less toxic povidone iodine and antibacterial peptide is added in boar semen dilutes and preserves liquid, is used for the dilution of economic animal sperm, preserves With killing bacterial virus.
The present invention in order to achieve the above-mentioned object of the invention, adopts the following technical scheme that：
A kind of dilution preservation liquid for killing animal semen virus and bacteria, by weight, including following component：Glucose 25- 40 parts, 2.5-3 parts of citric acid, 5-8 parts of trisodium citrate, 1.2-1.3 parts of sodium bicarbonate, disodium ethylene diamine tetraacetate 1.2-1.3 Part, 0.7-0.9 parts of potassium chloride, 2.8-3 parts of trishydroxymethylaminomethane, 0.05-0.08 parts of cysteine, povidone iodine 0.025- 0.030 part and 0-0.002 parts of antibacterial peptide.
Including following component preferably, by weight,：35-40 parts of glucose, 2.8-3 parts of citric acid, trisodium citrate 5- 6 parts, 1.25-1.3 parts of sodium bicarbonate, 1.25-1.3 parts of disodium ethylene diamine tetraacetate, 0.7-0.8 parts of potassium chloride, trihydroxy methyl ammonia 2.8-2.9 parts of methylmethane, 0.05-0.07 parts of cysteine, 0.025-0.030 parts of povidone iodine and antibacterial peptide 0.001-0.002 Part.
This dilution preserves liquid and is divided into two parts, dilutes nutrient solution part based on first part：Glucose, citric acid, lemon Lemon acid trisodium, sodium bicarbonate, disodium ethylene diamine tetraacetate, potassium chloride, trishydroxymethylaminomethane and cysteine, therein half Cystine is sperm protective；Second part is to kill virus and bacteria active ingredient：Povidone iodine.Inventive formulation passes through to three The control of the additional proportion of hydroxymethyl aminomethane, disodium ethylene diamine tetraacetate and potassium chloride, make dilution preserve liquid pH value be 6.20-6.30, osmotic pressure 347mOsm.
The pH that dilution of the present invention preserves liquid is 6.20-6.30, osmotic pressure 347mOsm.For the tight of sperm existence Lattice environmental requirement, the storage stability of sperm is best in this context.
The solvent that dilution preservation liquid of the present invention uses is distilled water.
Influence of the water quality for sperm motility and holding time is very big, and different regions different breeding enterprise water quality is all different, Key index therein such as pH, minerals and micronutrient levels, content of microorganisms, content of beary metal etc. influence sperm quality It is very big.The present invention overcomes disadvantage inconvenient for use, that effect is unstable using stable distilled water water quality.
Povidone iodine is one of common Operand of current livestock and poultry farm, is that iodine is loaded on strongly basic anionic resin A kind of contact-type disinfectant formed, can kill most of bacterium, fungi and virus.But in the concentration model to sperm safety In enclosing, povidone iodine has good effect to part bacterium and virus, but cannot kill common five kinds of virus inside sperm completely, Especially porcine pseudorabies virus and swine fever virus is ineffective.
Antibacterial peptide is a kind of biologically active micromolecule polypeptide generated through induction in organism, and molecular weight is 2000 ~7000 or so, it is made of 20~60 amino acid residues, has wide spectrum killing effect to bacterium and virus, and to sperm safety Property is very good, especially has good killing to sperm common virus especially swine fever virus, Pseudorabies virus, reproductive and respiratory syndrome virus Effect and povidone iodine, which are used cooperatively, strong complementarity.
It is further preferred that the antibacterial peptide is cockroach Endogenous antimicrobial polypeptide.
The present invention selects cockroach Endogenous antimicrobial polypeptide, amino acid sequence SEQIDNO:1.This antibacterial peptide has good Stability and broad-spectrum high efficacy antibacterial, it is antiviral the features such as, and mature production technology and perfect, production technology is in cockroach The amino acid sequence of antibiotic peptide is masterplate, and engineering bacterium expression is used after recombinating amino acid sequence with solid-phase peptide synthesis Fermentation, obtains recombined broad spectrum antibacterial peptide sterling.Cockroach Endogenous antimicrobial polypeptide is very good to sperm safety under concentration appropriate, And there are good killing effect, and low toxicity to sperm common virus especially swine fever virus, Pseudorabies virus, reproductive and respiratory syndrome virus Povidone iodine, which is used cooperatively, strong complementarity.
The dilution of the present invention preserves the production verification that liquid has obtained the whole nation more than 100 large and medium-sized pig raising enterprise, makes 80% is had more than with what Growth Results after product of the present invention greatly improved, generally reflects that sow breeding difficulty disease is greatly reduced, Disease incident is remarkably decreased, and pregnancy rate significantly improves, and nest farrowing pig's head number increases, and production performance generally improves, and has notable Economic benefit and social benefit.
Present invention employs preferred dilutions to preserve liquid basic components and povidone iodine disinfectant, and dilution is made to preserve formula of liquid Between stability it is best, along with unified stable distilled water water quality, overcome disadvantage inconvenient for use, that effect is unstable.
Specific implementation mode
The essentiality content of the present invention is described in further detail With reference to embodiment.
Embodiment 1
A kind of dilution preservation liquid for killing animal semen virus and bacteria, by weight, including following component：Glucose 25 Part, 2.5 parts of citric acid, 5 parts of trisodium citrate, 1.2 parts of sodium bicarbonate, 1.2 parts of disodium ethylene diamine tetraacetate, 0.7 part of potassium chloride, 0.025 part of 2.8 parts of trishydroxymethylaminomethane, 0.05 part of cysteine and povidone iodine.
Embodiment 2
A kind of dilution preservation liquid for killing animal semen virus and bacteria, by weight, including following component：Glucose 40 Part, 3 parts of citric acid, 8 parts of trisodium citrate, 1.3 parts of sodium bicarbonate, 1.3 parts of disodium ethylene diamine tetraacetate, 0.9 part of potassium chloride, three 0.002 part of 3 parts of hydroxymethyl aminomethane, 0.08 part of cysteine, 0.030 part of povidone iodine and antibacterial peptide.
The pH value that the dilution preserves liquid is 6.2, osmotic pressure 347mOsm.
The solvent that the dilution preserves liquid is distilled water.
Embodiment 3
A kind of dilution preservation liquid for killing animal semen virus and bacteria, by weight, including following component：Glucose 30 Part, 2.6 parts of citric acid, 6 parts of trisodium citrate, 1.22 parts of sodium bicarbonate, 1.22 parts of disodium ethylene diamine tetraacetate, potassium chloride 0.8 Part, 2.9 parts of trishydroxymethylaminomethane, 0.06 part of cysteine and 0.026 part of povidone iodine.
The pH value that the dilution preserves liquid is 6.3, osmotic pressure 347mOsm.
The solvent that the dilution preserves liquid is distilled water.
Embodiment 4
A kind of dilution preservation liquid for killing animal semen virus and bacteria, by weight, including following component：Glucose 35 Part, 2.8 parts of citric acid, 5 parts of trisodium citrate, 1.25 parts of sodium bicarbonate, 1.25 parts of disodium ethylene diamine tetraacetate, potassium chloride 0.7 Part, 2.8 parts of trishydroxymethylaminomethane, 0.05 part of cysteine, 0.025 part of povidone iodine and 0.001 part of antibacterial peptide.
The pH value that the dilution preserves liquid is 6.22, osmotic pressure 347mOsm.
The solvent that the dilution preserves liquid is distilled water.
Embodiment 5
A kind of dilution preservation liquid for killing animal semen virus and bacteria, by weight, including following component：Glucose 40 Part, 3 parts of citric acid, 6 parts of trisodium citrate, 1.3 parts of sodium bicarbonate, 1.3 parts of disodium ethylene diamine tetraacetate, 0.8 part of potassium chloride, three 0.002 part of 2.9 parts of hydroxymethyl aminomethane, 0.07 part of cysteine, 0.030 part of povidone iodine and cockroach Endogenous antimicrobial polypeptide.
The pH value that the dilution preserves liquid is 6.25, osmotic pressure 347mOsm.
The solvent that the dilution preserves liquid is distilled water.
Embodiment 6
A kind of dilution preservation liquid for killing animal semen virus and bacteria, by weight, including following component：Glucose 37 Part, 2.9 parts of citric acid, 6 parts of trisodium citrate, 1.25 parts of sodium bicarbonate, 1.25 parts of disodium ethylene diamine tetraacetate, potassium chloride 0.75 Part, 2.8 parts of trishydroxymethylaminomethane, 0.07 part of cysteine, 0.026 part of povidone iodine and cockroach Endogenous antimicrobial polypeptide 0.0015 part.
The pH value that the dilution preserves liquid is 6.26, osmotic pressure 347mOsm.
The solvent that the dilution preserves liquid is distilled water.
Embodiment 7
A kind of dilution preservation liquid for killing animal semen virus and bacteria, by weight, including following component：Glucose 37 Part, 2.9 parts of citric acid, 6 parts of trisodium citrate, 1.25 parts of sodium bicarbonate, 1.25 parts of disodium ethylene diamine tetraacetate, potassium chloride 0.75 In part, 2.8 parts of trishydroxymethylaminomethane, 0.08 part of cysteine, 1 part of vitamin C, 0.026 part of povidone iodine and cockroach 0.0012 part of antibiotic peptide.
The pH value that the dilution preserves liquid is 6.22, osmotic pressure 347mOsm.
The solvent that the dilution preserves liquid is distilled water.
Toxicology test
Toxicology test observes its acute toxicity, subacute toxicity and irritation toxicity data using animal experiment.
As a result：The agent of this sperm purifying sterilizing is all higher than 5000 m parts/k parts BW to rat and chmice acute LD50 of passing through mouth, Its stoste is nonirritant to lagophthalmos, intact skin and damaged skin；Dosage is in 1000 m parts/k parts BW hereinafter, with feminine gender Control group compares, and does not make significant difference to the weight of rat, blood routine and haemobiochemistry index, and each internal organs of experimental animal are not See apparent pathological change.Piglet by Oral sperm purifies protective agent stoste, and twice daily, 1ml/ is each, is administered within continuous two days, medication Iodine concentration is below maximum residue limit in front and back experiment piglet edible tissues, and povidone iodine is when pig uses without the off-drug period. It is being safe using toxicology under concentration that sperm, which purifies protective agent,.
Sperm purifying sterilizing agent stability of the present invention is good, and content of iodine rate of descent is less than after 37 DEG C of insulating boxs are placed three months 5%, content of iodine rate of descent is less than 10% within six months.
The present invention is good for porcine semen at normal temperature dilution preservation liquid stability, and the term of validity was kept in dark place up to 1 year in closed room temperature. PH is 6.20 ± 0.05, osmotic pressure 347mOsm.
Preparation method：In sterile workshop, the grape of formula ratio is sequentially added in 37oC 995.00ml sterilizing distilled waters Sugar, EDTA-Na2, trisodium citrate：, citric acid, sodium bicarbonate, Tris, potassium chloride and cysteine, side edged stirring at low speed, It is constant to maintain the temperature at 37 oC, waits for that temperature is down to room temperature after stirring evenly, the povidone iodine of formula ratio is added, stirring at low speed is equal It is aseptic subpackaged after even.
The product inspection project and operating method of the present invention
1, available iodine content detects
According to《Republic of China Veterinary Pharmacopoeia》（2005 editions）Effective iodine detecting method, this product is containing povidone iodine by effective Iodine (I) calculates, and should be the 0.85%~1.20% of labelled amount.
【Character】This product is colourless liquid.
【Differentiate】
(1) it takes this product 1~5 to drip, water 10ml is added to be dripped with starch indicator solution 1, i.e., aobvious bluish violet.
(2) this product 10ml is taken, is set in 50ml conical flasks (neck is sure not to stain in bottle), bottleneck covers one with starch indicator solution The filter paper of moistening is placed 80 seconds, not aobvious blue.
【It checks】PH value should be 6.20 ± 0.05.
2, different brands semen dilution preserves liquid and semen dilution preserves influence of the powder to the semen at normal temperature holding time
It buys four kinds of different brands semen dilutions and preserves liquid or semen dilution preservation powder in market（No. 1, No. 2, No. 3 and No. 4） It is for use after dilution.
The influence that 1 different diluent of table preserves porcine semen at normal temperature
As shown in Table 1, the effective holding time pole of present invention dilution preservation liquid is significantly higher than 1,2,3, No. 4 liquid（P ＜ 0.0 1）, survival index is also significantly greater than 1,2,3, No. 4 liquid（P ＜ 0.05）.
3, present invention dilution preserves liquid room temperature and preserves sperm breeding achievement
It is diluted with the present invention and preserves liquid dilution pig semen, 5d is preserved in 17 DEG C of insulating boxs, sperm motility is 0.5 or more Sperm is diluted to test for artificial insemination.Semen deposition sow 120 altogether, concrete outcome is shown in Table 2:As it can be seen that the present invention, which dilutes, preserves liquid Room temperature preserves the pig semen non-return rate of 5d, average nest litter size is apparently higher than 1,2,3, No. 4 liquid.
2 different diluent room temperature of table preserves the pig semen non-return rate of 5d, average nest litter size
Sequence table
<110>Chengdu Mei Qiang veterinary arts Services Co., Ltd
<120>A kind of dilution preservation liquid for killing animal semen virus and bacteria
<160>1
<170>PatentInversion3.3
<210>1
<211>37
<212>PRT
<213>Cockroach Endogenous antimicrobial polypeptide
<400>1
Lys Trp Lys Val Phe Lys Lys Ile glu Lys Met gly Arg Asn Ile Arg
1 5 10 15
Asn gly Ile Val Lys Ala gly Pro Ala Ile Ala Val Leu gly glu Ala
20 25 30
Lys Ala Lys Ala Leu
35

Claims (3)

1. a kind of dilution for killing pig semen virus and bacteria preserves liquid, it is characterised in that：By weight, it consists of the following compositions： 35-40 parts of glucose, 2.8-3 parts of citric acid, 5-6 parts of trisodium citrate, 1.25-1.3 parts of sodium bicarbonate, ethylenediamine tetra-acetic acid two 1.25-1.3 parts of sodium, 2.8-2.9 parts of trishydroxymethylaminomethane, 0.05-0.07 parts of cysteine, gathers 0.7-0.8 parts of potassium chloride Tie up 0.001-0.002 parts of 0.025-0.030 parts of ketone iodine and antibacterial peptide；
The pH value that the dilution preserves liquid is 6.2-6.3, osmotic pressure 347mOsm.
2. a kind of dilution for killing pig semen virus and bacteria according to claim 1 preserves liquid, it is characterised in that：It is described dilute It is cockroach Endogenous antimicrobial polypeptide to release and preserve the antibacterial peptide of liquid.
3. a kind of dilution for killing pig semen virus and bacteria according to claim 1 preserves liquid, it is characterised in that：It is described dilute It is distilled water to release and preserve the solvent of liquid.
CN201610439771.XA 2016-06-20 2016-06-20 A kind of dilution preservation liquid for killing animal semen virus and bacteria Expired - Fee Related CN105994251B (en)

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CN107912422A (en) * 2017-11-21 2018-04-17 广东中农联生物制药有限公司 A kind of dilution for pig semen and preparation method thereof
CN108588014A (en) * 2018-03-15 2018-09-28 广州伯瑞丁生物新技术开发有限公司 A kind of Boar spermatozoa culture medium and preparation method thereof
CN109938008A (en) * 2019-03-20 2019-06-28 上海创宏生物科技有限公司 A kind of animal semen inorganic agent and its application method
CN111869658A (en) * 2020-08-22 2020-11-03 南京健邦锦源医疗科技有限公司 Inactivated virus preservation solution and preparation method thereof
CN114794085A (en) * 2022-06-08 2022-07-29 杭州东源生物科技有限公司 Diluent for preserving pig semen at low temperature and preparation method thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101168564A (en) * 2007-10-22 2008-04-30 中国科学院昆明动物研究所 Human antibiotic peptide and use for derivative thereof
CN104304237A (en) * 2014-11-11 2015-01-28 成都美强兽医技术服务有限公司 Safe and efficient animal semen disinfector and preparation method therefor
CN105010304A (en) * 2014-04-23 2015-11-04 马乃祥 Prescription composition of pig antiviral semen diluent
CN105325401A (en) * 2015-11-10 2016-02-17 天津市畜牧兽医研究所 Fresh-pig-semen preserving long-acting dilution powder, preparing method thereof and application thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101168564A (en) * 2007-10-22 2008-04-30 中国科学院昆明动物研究所 Human antibiotic peptide and use for derivative thereof
CN105010304A (en) * 2014-04-23 2015-11-04 马乃祥 Prescription composition of pig antiviral semen diluent
CN104304237A (en) * 2014-11-11 2015-01-28 成都美强兽医技术服务有限公司 Safe and efficient animal semen disinfector and preparation method therefor
CN105325401A (en) * 2015-11-10 2016-02-17 天津市畜牧兽医研究所 Fresh-pig-semen preserving long-acting dilution powder, preparing method thereof and application thereof

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