RO99451B1 - Procedeu de preparare a muteinelor fara cisteina ale factorului uman de necroza a tumorilor - Google Patents

Procedeu de preparare a muteinelor fara cisteina ale factorului uman de necroza a tumorilor

Info

Publication number
RO99451B1
RO99451B1 RO124923A RO12492386A RO99451B1 RO 99451 B1 RO99451 B1 RO 99451B1 RO 124923 A RO124923 A RO 124923A RO 12492386 A RO12492386 A RO 12492386A RO 99451 B1 RO99451 B1 RO 99451B1
Authority
RO
Romania
Prior art keywords
necrosis factor
cystein
preparing
tumour necrosis
human tumour
Prior art date
Application number
RO124923A
Other languages
English (en)
Inventor
David F. Mark
Leo S. Lin
Shi-Sa Yu Lu
Alice M. Wang
Original Assignee
Cetus Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cetus Corporation filed Critical Cetus Corporation
Priority to RO124923A priority Critical patent/RO99451B1/ro
Publication of RO99451B1 publication Critical patent/RO99451B1/ro

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  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Procedeul de preparare a muteinelor prevede substituirea reziduurilor de cisteina din pozitiile 67 si/sau 101 ale secventei de aminoacizi cu un alt aminoacid ales din grupa constând în serina, treonina, glicina, alanina, valina, leucina, izoleucina,histidina, tirozina,fenilalamina triptofan sau metionina, muteinele obtinute sunt lipsite de capacitatea de a forma legaturi disulfidice, si sunt utilizate pentru transformarea unei gazde bacteriene adecvate în care are loc replicarea heteroduplexuluisi din care se poate izola factorul de necroza a tumorilor fara continut de cisteina.
RO124923A 1986-10-03 1986-10-03 Procedeu de preparare a muteinelor fara cisteina ale factorului uman de necroza a tumorilor RO99451B1 (ro)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RO124923A RO99451B1 (ro) 1986-10-03 1986-10-03 Procedeu de preparare a muteinelor fara cisteina ale factorului uman de necroza a tumorilor

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RO124923A RO99451B1 (ro) 1986-10-03 1986-10-03 Procedeu de preparare a muteinelor fara cisteina ale factorului uman de necroza a tumorilor

Publications (1)

Publication Number Publication Date
RO99451B1 true RO99451B1 (ro) 1990-08-30

Family

ID=40907518

Family Applications (1)

Application Number Title Priority Date Filing Date
RO124923A RO99451B1 (ro) 1986-10-03 1986-10-03 Procedeu de preparare a muteinelor fara cisteina ale factorului uman de necroza a tumorilor

Country Status (1)

Country Link
RO (1) RO99451B1 (ro)

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