RO96490B1 - Procedeu de obtinere a antigenului echinoccocus granulosus - Google Patents
Procedeu de obtinere a antigenului echinoccocus granulosusInfo
- Publication number
- RO96490B1 RO96490B1 RO124674A RO12467486A RO96490B1 RO 96490 B1 RO96490 B1 RO 96490B1 RO 124674 A RO124674 A RO 124674A RO 12467486 A RO12467486 A RO 12467486A RO 96490 B1 RO96490 B1 RO 96490B1
- Authority
- RO
- Romania
- Prior art keywords
- echinoccocus
- preparing
- azide
- granulosus antigen
- granulosus
- Prior art date
Links
- 239000000427 antigen Substances 0.000 title 1
- 102000036639 antigens Human genes 0.000 title 1
- 108091007433 antigens Proteins 0.000 title 1
- 238000004519 manufacturing process Methods 0.000 title 1
- 206010014096 Echinococciasis Diseases 0.000 abstract 1
- 208000009366 Echinococcosis Diseases 0.000 abstract 1
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 abstract 1
- 239000008351 acetate buffer Substances 0.000 abstract 1
- 150000001540 azides Chemical class 0.000 abstract 1
- 238000000502 dialysis Methods 0.000 abstract 1
- 238000001704 evaporation Methods 0.000 abstract 1
- 230000008020 evaporation Effects 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 239000008363 phosphate buffer Substances 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 239000013049 sediment Substances 0.000 abstract 1
Landscapes
- Peptides Or Proteins (AREA)
- Medicinal Preparation (AREA)
Abstract
Procedeul, conform inventiei, consta în aceea ca un volum din lichidul recoltat din cavitatea chistului hidatic se filtreaza, se concentreaza de 10 ori prin evaporare în sacul de dializa, dupa care se dializeaza în tampon acetat 0,01 M la pH=5, se centrifugheaza, iar sedimentul obtinut este reluat în 0,020 vol. tampon fosfat 0,02 M la pH=8, se verifica concentratia proteica, care trebuie sa fie 1,8...2,5 mg/ml, si se trateaza cu azida de sodiu pâna la obtinerea unei concentratii de 0,5% azida.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RO124674A RO96490B1 (ro) | 1986-09-02 | 1986-09-02 | Procedeu de obtinere a antigenului echinoccocus granulosus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RO124674A RO96490B1 (ro) | 1986-09-02 | 1986-09-02 | Procedeu de obtinere a antigenului echinoccocus granulosus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| RO96490B1 true RO96490B1 (ro) | 1989-03-02 |
Family
ID=40905194
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RO124674A RO96490B1 (ro) | 1986-09-02 | 1986-09-02 | Procedeu de obtinere a antigenului echinoccocus granulosus |
Country Status (1)
| Country | Link |
|---|---|
| RO (1) | RO96490B1 (ro) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103217524A (zh) * | 2012-12-28 | 2013-07-24 | 新疆维吾尔自治区实验动物研究中心 | 适用于检测泡型包虫病抗体的囊型包虫病囊液脱酯抗原及其制备方法 |
-
1986
- 1986-09-02 RO RO124674A patent/RO96490B1/ro unknown
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103217524A (zh) * | 2012-12-28 | 2013-07-24 | 新疆维吾尔自治区实验动物研究中心 | 适用于检测泡型包虫病抗体的囊型包虫病囊液脱酯抗原及其制备方法 |
| CN103217524B (zh) * | 2012-12-28 | 2015-07-01 | 新疆维吾尔自治区实验动物研究中心 | 适用于检测泡型包虫病抗体的囊型包虫病囊液脱酯抗原及其制备方法 |
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