RO96322B1 - Method for the quantitative determination of myoglobin - Google Patents
Method for the quantitative determination of myoglobinInfo
- Publication number
- RO96322B1 RO96322B1 RO124734A RO12473486A RO96322B1 RO 96322 B1 RO96322 B1 RO 96322B1 RO 124734 A RO124734 A RO 124734A RO 12473486 A RO12473486 A RO 12473486A RO 96322 B1 RO96322 B1 RO 96322B1
- Authority
- RO
- Romania
- Prior art keywords
- parts
- myoglobin
- hours
- added
- same buffer
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 2
- 102000036675 Myoglobin Human genes 0.000 title 1
- 108010062374 Myoglobin Proteins 0.000 title 1
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 abstract 3
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 abstract 2
- 239000000872 buffer Substances 0.000 abstract 2
- 239000000243 solution Substances 0.000 abstract 2
- XZKIHKMTEMTJQX-UHFFFAOYSA-N 4-Nitrophenyl Phosphate Chemical compound OP(O)(=O)OC1=CC=C([N+]([O-])=O)C=C1 XZKIHKMTEMTJQX-UHFFFAOYSA-N 0.000 abstract 1
- 229920001213 Polysorbate 20 Polymers 0.000 abstract 1
- 238000003556 assay Methods 0.000 abstract 1
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 abstract 1
- 239000002953 phosphate buffered saline Substances 0.000 abstract 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 abstract 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Metoda de determinare, conform inventiei, consta în aceea ca, 1 parte solutie de anticorpi antimioglobina diluata conform unui litru determinat cu solutia tampon fosfat salin cu pH=7,4 ce contine Tween 20 si azida de sodiu, se pune într-un godeu si se tine 2 h la 37 degree C si 16...18 h la 4 degree C, dupa care se spala cu acelasi tampon, se adauga apoi 0,5 parti din proba de cercetat si se incubeaza 60 min la 37 degree C, se adauga, în continuare, 0,5 parti conjugat mioglobina-fosfataza alcalina, diluat tot conform unui titru determinat, se omogenizeaza si se lasa 2 h la 37 degree C, se spala cu acelasi tampon, se adauga 1 parte solutie 0,1% para-nitrfenilfosfat si se lasa 1 h la 37 degree C, se adauga apoi 0,25 parti solutie NaOH 1 N, dupa care se interpreteaza spectrofotometric dupa metode cunoscute, partile fiind exprimate în volum. The assay method according to the invention consists in that 1 part solution of diluted anti-myoglobin antibody per liter determined with phosphate buffered saline pH 7.4 containing Tween 20 and sodium azide is placed in a well and is kept for 2 hours at 37 ° C and 16-18 hours at 4 ° C, then washed with the same buffer, then 0.5 parts of the sample is added and incubated for 60 minutes at 37 ° C. add further 0.5 parts of alkaline myoglobin-phosphatase conjugate, diluted also according to a determined titre, homogenize and leave for 2 hours at 37 ° C, wash with the same buffer, add 1 part 0.1% para -nitrophenylphosphate and left for 1 hour at 37 ° C, 0.25 parts of 1 N NaOH solution is then added, and then spectrophotometrically by known methods, the parts being expressed in volume.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RO124734A RO96322B1 (en) | 1986-09-09 | 1986-09-09 | Method for the quantitative determination of myoglobin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RO124734A RO96322B1 (en) | 1986-09-09 | 1986-09-09 | Method for the quantitative determination of myoglobin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RO96322A2 RO96322A2 (en) | 1988-09-15 |
| RO96322B1 true RO96322B1 (en) | 1988-09-17 |
Family
ID=20119826
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RO124734A RO96322B1 (en) | 1986-09-09 | 1986-09-09 | Method for the quantitative determination of myoglobin |
Country Status (1)
| Country | Link |
|---|---|
| RO (1) | RO96322B1 (en) |
-
1986
- 1986-09-09 RO RO124734A patent/RO96322B1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| RO96322A2 (en) | 1988-09-15 |
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