RO102778B1 - Producing method of human anti-transpherine serum - Google Patents

Producing method of human anti-transpherine serum

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Publication number
RO102778B1
RO102778B1 RO135551A RO13555188A RO102778B1 RO 102778 B1 RO102778 B1 RO 102778B1 RO 135551 A RO135551 A RO 135551A RO 13555188 A RO13555188 A RO 13555188A RO 102778 B1 RO102778 B1 RO 102778B1
Authority
RO
Romania
Prior art keywords
serum
sodium
transpherine
human anti
producing method
Prior art date
Application number
RO135551A
Other languages
Romanian (ro)
Other versions
RO102778A2 (en
Inventor
Aurel Ionita
Constantin Ionita
Emil Ionita
Original Assignee
Institutul Cantacuzino
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institutul Cantacuzino filed Critical Institutul Cantacuzino
Priority to RO135551A priority Critical patent/RO102778B1/en
Publication of RO102778A2 publication Critical patent/RO102778A2/en
Publication of RO102778B1 publication Critical patent/RO102778B1/en

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  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Procedeul conform inventiei, consta în aceea ca 1 volum urina, ce contine 50 mg % transfeina, se amesteca cu o cantitate corespunzatoare de ser monospecific anti-transferina, se lasa 1 h la 37 degree C si se centrifugheaza, precipitatul obtinut se spala cu solutie 0,05 % dodecilsulfat de sodiu si apoi se reia la 30 degree C într-o cantitate minima de tampon veronal de sodiu 0,05 M pH=8, ce contine 0,5 % dodecilsulfat de sodiu, dupa care se trece cu o rata de 10 ml/h pe o coloana cromatografica Bio-Gel P - 100 extrafina, adusa la temperatura de 30 degree C, se recolteaza al 2-lea pic care, dupa îndepartarea dodecilsulfatului de sodiu si concentrare prinmetode cunoscute , este folosit pentru imunizarea animalelor în vederea obtinerii serului.The process according to the invention consists in that 1 volume of urine containing 50 mg% of the transfein is mixed with an appropriate amount of monospecific anti-transferrin serum, left for 1 hour at 37 ° C and centrifuged, the precipitate obtained is washed with solution 0.05% sodium dodecylsulfate and then resuspended at 30 ° C in a minimal amount of 0.05M sodium veranic buffer pH = 8, containing 0.5% sodium dodecylsulfate, followed by a rate of 10 ml / h on a Bio-Gel P-100 chromatographic column, extracted at 30 ° C, 2 nd peak is harvested which, after removal of sodium dodecyl sulfate and concentration by known methods, is used to immunize the animals in to get the serum.

RO135551A 1988-10-18 1988-10-18 Producing method of human anti-transpherine serum RO102778B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RO135551A RO102778B1 (en) 1988-10-18 1988-10-18 Producing method of human anti-transpherine serum

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RO135551A RO102778B1 (en) 1988-10-18 1988-10-18 Producing method of human anti-transpherine serum

Publications (2)

Publication Number Publication Date
RO102778A2 RO102778A2 (en) 1991-10-01
RO102778B1 true RO102778B1 (en) 1991-12-31

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ID=20122658

Family Applications (1)

Application Number Title Priority Date Filing Date
RO135551A RO102778B1 (en) 1988-10-18 1988-10-18 Producing method of human anti-transpherine serum

Country Status (1)

Country Link
RO (1) RO102778B1 (en)

Also Published As

Publication number Publication date
RO102778A2 (en) 1991-10-01

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