RO102778A2 - Producing method of human anti-transpherine serum - Google Patents

Producing method of human anti-transpherine serum

Info

Publication number
RO102778A2
RO102778A2 RO1988135551A RO13555188A RO102778A2 RO 102778 A2 RO102778 A2 RO 102778A2 RO 1988135551 A RO1988135551 A RO 1988135551A RO 13555188 A RO13555188 A RO 13555188A RO 102778 A2 RO102778 A2 RO 102778A2
Authority
RO
Romania
Prior art keywords
serum
transpherine
human anti
producing method
antitransferrin
Prior art date
Application number
RO1988135551A
Other languages
Romanian (ro)
Other versions
RO102778B1 (en
Inventor
Ro Ionita Aurel
Ro Ionita Constantin
Ro Ionita Emil
Original Assignee
Institutul Cantacuzino, Bucuresti, Ro
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institutul Cantacuzino, Bucuresti, Ro filed Critical Institutul Cantacuzino, Bucuresti, Ro
Priority to RO135551A priority Critical patent/RO102778B1/en
Publication of RO102778A2 publication Critical patent/RO102778A2/en
Publication of RO102778B1 publication Critical patent/RO102778B1/en

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  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

For the prepn. of antitransferrin human serum, 1 vol. of urine, contg. 50 mg of transferrin is mixed with a corresp. quantity of mono-specific antitransferrin specific serum. The mixt. is allowed to stand at 37 deg.C for 1 hr. prior to centrifuging. The ppte. obtd. is washed with 0.05% to Na-dodecyl-sulphate soln. and taken up into a least vol. of 0.05 M Na-veronal buffer contg. 0.5% Na-dodecyl-sulphate at 30 deg.C and pH. The liq. is passed through an extrafine Bio-Gel P-1000 chromatographic column at 30 deg.C and at a rate of 10 mls/hr. The second drop is collected, purified, concentrated and used to immunise animals with the urine to prepare the serum.
RO135551A 1988-10-18 1988-10-18 Producing method of human anti-transpherine serum RO102778B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RO135551A RO102778B1 (en) 1988-10-18 1988-10-18 Producing method of human anti-transpherine serum

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RO135551A RO102778B1 (en) 1988-10-18 1988-10-18 Producing method of human anti-transpherine serum

Publications (2)

Publication Number Publication Date
RO102778A2 true RO102778A2 (en) 1991-10-01
RO102778B1 RO102778B1 (en) 1991-12-31

Family

ID=20122658

Family Applications (1)

Application Number Title Priority Date Filing Date
RO135551A RO102778B1 (en) 1988-10-18 1988-10-18 Producing method of human anti-transpherine serum

Country Status (1)

Country Link
RO (1) RO102778B1 (en)

Also Published As

Publication number Publication date
RO102778B1 (en) 1991-12-31

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