RO96320B1 - Procedeu de obtinere a serului anti alfa 1-glicoproteina acida umana - Google Patents
Procedeu de obtinere a serului anti alfa 1-glicoproteina acida umanaInfo
- Publication number
- RO96320B1 RO96320B1 RO125728A RO12572886A RO96320B1 RO 96320 B1 RO96320 B1 RO 96320B1 RO 125728 A RO125728 A RO 125728A RO 12572886 A RO12572886 A RO 12572886A RO 96320 B1 RO96320 B1 RO 96320B1
- Authority
- RO
- Romania
- Prior art keywords
- preparing
- human anti
- centrifuged
- rpm
- concentrated
- Prior art date
Links
Landscapes
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
Procedeul conform inventiei consta în aceea ca 2 vol de urina umana, ce contin circa 30 mg alfa1-glicoproteina acida/100 ml, se concentreaza pâna la obtinerea unui volum de 20 de ori mai mic, se centrifugheaza la 6÷7000 rot/min, timp de 30 min, si apoi se adauga, sub agitare, sulfat de amoniu pâna la obtinerea unei concentratii de 30 g/100 ml solutie, se lasa 24 h la temperatura camerei, se centrifugheaza, iar la 10000 rot/min, timp de 30 min, si supernatantul obtinut se concentreaza pâna la obtinerea unui volum de 40 ori mai mic fata de cel al urinii concentrate înainte de precipitare, se trece prin coloana cromatografica de con A-Sefaroza 4 B, se elueaza cu tampon acetat si, în final se imunizeaza animalele mari, în mod cunoscut si se recolteaza serul imun.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
RO125728A RO96320B1 (ro) | 1986-12-06 | 1986-12-06 | Procedeu de obtinere a serului anti alfa 1-glicoproteina acida umana |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
RO125728A RO96320B1 (ro) | 1986-12-06 | 1986-12-06 | Procedeu de obtinere a serului anti alfa 1-glicoproteina acida umana |
Publications (2)
Publication Number | Publication Date |
---|---|
RO96320A2 RO96320A2 (ro) | 1989-02-28 |
RO96320B1 true RO96320B1 (ro) | 1989-03-02 |
Family
ID=20120125
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
RO125728A RO96320B1 (ro) | 1986-12-06 | 1986-12-06 | Procedeu de obtinere a serului anti alfa 1-glicoproteina acida umana |
Country Status (1)
Country | Link |
---|---|
RO (1) | RO96320B1 (ro) |
-
1986
- 1986-12-06 RO RO125728A patent/RO96320B1/ro unknown
Also Published As
Publication number | Publication date |
---|---|
RO96320A2 (ro) | 1989-02-28 |
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