RO95326A2 - PROCEDURE FOR PREPARING THE TREPONEMIC ANTIGEN - Google Patents
PROCEDURE FOR PREPARING THE TREPONEMIC ANTIGENInfo
- Publication number
- RO95326A2 RO95326A2 RO86123942A RO12394286A RO95326A2 RO 95326 A2 RO95326 A2 RO 95326A2 RO 86123942 A RO86123942 A RO 86123942A RO 12394286 A RO12394286 A RO 12394286A RO 95326 A2 RO95326 A2 RO 95326A2
- Authority
- RO
- Romania
- Prior art keywords
- centrifuging
- phosphate
- supernatant
- days
- stand
- Prior art date
Links
- 239000000427 antigen Substances 0.000 title abstract 2
- 102000036639 antigens Human genes 0.000 title abstract 2
- 108091007433 antigens Proteins 0.000 title abstract 2
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 abstract 3
- 239000000872 buffer Substances 0.000 abstract 2
- 239000011780 sodium chloride Substances 0.000 abstract 2
- 239000006228 supernatant Substances 0.000 abstract 2
- CJNZAXGUTKBIHP-UHFFFAOYSA-N 2-iodobenzoic acid Chemical compound OC(=O)C1=CC=CC=C1I CJNZAXGUTKBIHP-UHFFFAOYSA-N 0.000 abstract 1
- 229920002307 Dextran Polymers 0.000 abstract 1
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 abstract 1
- MBBZMMPHUWSWHV-BDVNFPICSA-N N-methylglucamine Chemical class CNC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO MBBZMMPHUWSWHV-BDVNFPICSA-N 0.000 abstract 1
- 241000283973 Oryctolagus cuniculus Species 0.000 abstract 1
- 229910019142 PO4 Inorganic materials 0.000 abstract 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 1
- 241000589886 Treponema Species 0.000 abstract 1
- 125000000218 acetic acid group Chemical group C(C)(=O)* 0.000 abstract 1
- 244000052616 bacterial pathogen Species 0.000 abstract 1
- 239000000706 filtrate Substances 0.000 abstract 1
- 239000011521 glass Substances 0.000 abstract 1
- 229960002897 heparin Drugs 0.000 abstract 1
- 229920000669 heparin Polymers 0.000 abstract 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 abstract 1
- 239000010452 phosphate Substances 0.000 abstract 1
- 239000008363 phosphate buffer Substances 0.000 abstract 1
- 239000013049 sediment Substances 0.000 abstract 1
- 210000001550 testis Anatomy 0.000 abstract 1
- RTKIYNMVFMVABJ-UHFFFAOYSA-L thimerosal Chemical compound [Na+].CC[Hg]SC1=CC=CC=C1C([O-])=O RTKIYNMVFMVABJ-UHFFFAOYSA-L 0.000 abstract 1
- 229940033663 thimerosal Drugs 0.000 abstract 1
Landscapes
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
A treponema antigen is prepd. from a strain of Treponeuca Pallidum registered under No 13156 in the collection of the Cautacuzino Triotitute in Bucherest. This is injected in the testicles of rabbits. The infected tissues are collected after ten days and are suspended in a 100 i.u-s heparin/ml containing, phosphate-saline buffer. The suspn. is allowed to stand for 45 mins at 37 deg.C. The supernatant is decanted, centrifuged and filtered through a medium porosity fritted glass filter. The filtrate is treated by a mixt. comprising a methyl-glucamine salt of N, N'-di:acetyl -3-5-di:amino-2,4,6 -tri:iodobenzoic-acid and poly-dextran of 4,000,000 Mol.Wt.; followed by centrifuging. Formaldehyde is added to the supernatant and allowed to stand for 7 days at 4 deg.C prior to centrifuging. The sediment is rinsed with phosphate saline buffer. Final concn. of 2 x 10 power 7 germs/ml phosphate-buffer is set by centrifuging. 0.75% glycocol, 0.5% sodium-glutenate and 0.02% Thimerosal is added. The prepn. is proportioned in vials, and hyophilised. Frozen at -58 deg.C and dried for 48 hours up to 30 deg.C max. Closure of vial occurs in Ar atmos.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RO123942A RO95326B1 (en) | 1986-06-30 | 1986-06-30 | Process for preparing treponema antigen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RO123942A RO95326B1 (en) | 1986-06-30 | 1986-06-30 | Process for preparing treponema antigen |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RO95326A2 true RO95326A2 (en) | 1989-05-30 |
| RO95326B1 RO95326B1 (en) | 1989-05-31 |
Family
ID=20119579
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RO123942A RO95326B1 (en) | 1986-06-30 | 1986-06-30 | Process for preparing treponema antigen |
Country Status (1)
| Country | Link |
|---|---|
| RO (1) | RO95326B1 (en) |
-
1986
- 1986-06-30 RO RO123942A patent/RO95326B1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| RO95326B1 (en) | 1989-05-31 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Coller et al. | Thromboerythrocytes. In vitro studies of a potential autologous, semi-artificial alternative to platelet transfusions. | |
| US4455256A (en) | Bone morphogenetic protein | |
| FI60022B (en) | FRAMEWORK FOR RELEASE OF RENTAL INJECTED GAMMAGLOBULIN | |
| EP0461177A4 (en) | Human intra-acrosomal sperm antigen for use in a contraceptive vaccine | |
| IE832394L (en) | Immunogenic protein or peptide complex vaccine. | |
| SE9500418D0 (en) | Novel attenuated pseudomonas aeruginosa strains | |
| US4027010A (en) | Antistaphylococcous human immunoglobulin and method of preparing same | |
| ES469328A1 (en) | Glycoprotein and process for isolating it | |
| CA1058075A (en) | Process of improving the compatibility of gamma globulins | |
| US4124702A (en) | Polynucleotides active as inducers of interferon production in living animal cells | |
| Traub et al. | Anticomplementary, anticoagulatory, and serum-protein precipitating activity of sodium polyanetholsulfonate | |
| ATE82985T1 (en) | STRUCTURAL PHOSPHOPROTEIN (PP 150) OF HUMAN CYTOMEGALOVIRUS, ITS PRODUCTION AND USE. | |
| Grumet et al. | Reversal of profilin inhibition of actin polymerization invitro by erythrocyte cytochalasin-binding complexes and cross-linked actin nuclei | |
| US4285933A (en) | Process for concentrating blood coagulation Factor XIII derived from human placentae | |
| US3449316A (en) | Process for the purification of gamma globulin employing bentonite | |
| RU2051054C1 (en) | Method of preparing preparation of human antiherpetic immunoglobulin | |
| Tsuiki et al. | Bovine submaxillary mucin | |
| NO960766L (en) | New protein and process for its preparation | |
| EP0241136A3 (en) | Human class 1 heparin-binding growth factor | |
| US3627642A (en) | Lysozyme salts | |
| Klein et al. | Relevance of antimitochondrial antibodies (anti-M7) in cardiac diseases | |
| SU448864A1 (en) | The method of obtaining antilles to the Australian antigen | |
| Shumway et al. | Survival in immune and nonimmune rabbits of Cr51-labeled erythrocytes modified by bacterial antigen | |
| WO1988008004A1 (en) | Extraction of factor viii | |
| RO95326B1 (en) | Process for preparing treponema antigen |