Institutul De Cercetari Veterinare Si Biopreparate "Pasteur"
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institutul De Cercetari Veterinare Si Biopreparate "Pasteur"filedCriticalInstitutul De Cercetari Veterinare Si Biopreparate "Pasteur"
Priority to RO108235ApriorityCriticalpatent/RO84442B1/en
Publication of RO84442A2publicationCriticalpatent/RO84442A2/en
Publication of RO84442B1publicationCriticalpatent/RO84442B1/en
Preparation Of Compounds By Using Micro-Organisms
(AREA)
Saccharide Compounds
(AREA)
Abstract
Inventia se refera la un procedeu de obtinere a nicotinamidadenindinucleotidei din drojdie de bere supusa unui proces de extragere cu apa, extractul de drojdie se trateaza cu acid clorhidric, se clarifica cu o solutie de floculant macromolecular, sepurifica pe o coloana cu un copolimer macroporos adsorbant, se trateaza la rece cu carbune activ, dupa centrifugare NAD-ul se extrage de 5 ori consecutiv la temperatura de 50...55 degree C cu un amestec constituit din acetat de etil, acetona, apa si amoniac 10%, dupa care se precipita cu acetona la o temperatura sub -10 degree C.The invention relates to a process for obtaining nicotinamide adenine dinucleotide from beer yeast subjected to a water extraction process, the yeast extract is treated with hydrochloric acid, clarified with a macromolecular flocculant solution, sepurified on a column with an adsorbent macroporous copolymer, is treated cold with activated charcoal, after centrifugation the NAD is extracted 5 times consecutively at 50-55 ° C with a mixture consisting of ethyl acetate, acetone, water and 10% ammonia, after which it precipitates with acetone at a temperature below -10 ° C.
RO108235A1982-07-231982-07-23Process for obtaining nicotinamidadenindinucleotide
RO84442B1
(en)
Biochemistry of germination and low-temperature stratification of Abies sachalinesis FR. SCHM. seeds.(1) On the change of auxin and inhibitor in the seeds during low-temperature stratification
Studies on the Mechanisms of Resistance of Rice Plants Against Xanthomonas oryzae IV. Extraction and Partial Purification of Antibacterial Substances from Infected Leaves