RO137903A2 - Biostimulant product based on proteinaceous substances, preparation process and application method - Google Patents
Biostimulant product based on proteinaceous substances, preparation process and application method Download PDFInfo
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- RO137903A2 RO137903A2 ROA202200412A RO202200412A RO137903A2 RO 137903 A2 RO137903 A2 RO 137903A2 RO A202200412 A ROA202200412 A RO A202200412A RO 202200412 A RO202200412 A RO 202200412A RO 137903 A2 RO137903 A2 RO 137903A2
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- biostimulant
- concentration
- substances
- product
- nitrogen
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- 239000000126 substance Substances 0.000 title claims abstract description 36
- 238000000034 method Methods 0.000 title claims abstract description 24
- 238000002360 preparation method Methods 0.000 title 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 40
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 20
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims abstract description 15
- 239000000243 solution Substances 0.000 claims abstract description 14
- 238000003756 stirring Methods 0.000 claims abstract description 13
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims abstract description 11
- 239000011777 magnesium Substances 0.000 claims abstract description 10
- 229910052802 copper Inorganic materials 0.000 claims abstract description 9
- 239000010949 copper Substances 0.000 claims abstract description 9
- 229910052742 iron Inorganic materials 0.000 claims abstract description 9
- 229910052749 magnesium Inorganic materials 0.000 claims abstract description 9
- 239000011701 zinc Substances 0.000 claims abstract description 9
- 229910052725 zinc Inorganic materials 0.000 claims abstract description 9
- CHWRSCGUEQEHOH-UHFFFAOYSA-N potassium oxide Chemical compound [O-2].[K+].[K+] CHWRSCGUEQEHOH-UHFFFAOYSA-N 0.000 claims abstract description 8
- DLYUQMMRRRQYAE-UHFFFAOYSA-N tetraphosphorus decaoxide Chemical compound O1P(O2)(=O)OP3(=O)OP1(=O)OP2(=O)O3 DLYUQMMRRRQYAE-UHFFFAOYSA-N 0.000 claims abstract description 8
- 229910052750 molybdenum Inorganic materials 0.000 claims abstract description 7
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 claims abstract description 6
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims abstract description 6
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims abstract description 6
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 claims abstract description 6
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims abstract description 6
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims abstract description 6
- 229910052796 boron Inorganic materials 0.000 claims abstract description 6
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 claims abstract description 6
- 229910017604 nitric acid Inorganic materials 0.000 claims abstract description 6
- ZOKXTWBITQBERF-UHFFFAOYSA-N Molybdenum Chemical compound [Mo] ZOKXTWBITQBERF-UHFFFAOYSA-N 0.000 claims abstract description 5
- 238000005903 acid hydrolysis reaction Methods 0.000 claims abstract description 5
- 239000002639 bone cement Substances 0.000 claims abstract description 5
- 229910017052 cobalt Inorganic materials 0.000 claims abstract description 5
- 239000010941 cobalt Substances 0.000 claims abstract description 5
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 claims abstract description 5
- 238000001816 cooling Methods 0.000 claims abstract description 5
- 239000011733 molybdenum Substances 0.000 claims abstract description 5
- 229910001950 potassium oxide Inorganic materials 0.000 claims abstract description 5
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims abstract description 4
- 239000007864 aqueous solution Substances 0.000 claims abstract description 3
- 238000009331 sowing Methods 0.000 claims abstract description 3
- 238000005507 spraying Methods 0.000 claims abstract description 3
- 108090000623 proteins and genes Proteins 0.000 claims description 15
- 102000004169 proteins and genes Human genes 0.000 claims description 15
- 239000003337 fertilizer Substances 0.000 claims description 8
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Chemical compound [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 claims description 8
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 7
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 7
- 235000011007 phosphoric acid Nutrition 0.000 claims description 6
- 102000015636 Oligopeptides Human genes 0.000 claims description 5
- 108010038807 Oligopeptides Proteins 0.000 claims description 5
- 150000003839 salts Chemical class 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 4
- 125000001477 organic nitrogen group Chemical group 0.000 claims description 4
- 229910000027 potassium carbonate Inorganic materials 0.000 claims description 4
- 239000011593 sulfur Substances 0.000 claims description 4
- 229910052717 sulfur Inorganic materials 0.000 claims description 4
- 229910021094 Co(NO3)2-6H2O Inorganic materials 0.000 claims description 3
- 235000015320 potassium carbonate Nutrition 0.000 claims description 3
- 230000003472 neutralizing effect Effects 0.000 claims description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 abstract description 3
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 abstract description 3
- 235000011114 ammonium hydroxide Nutrition 0.000 abstract description 3
- 239000005864 Sulphur Substances 0.000 abstract description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 150000007524 organic acids Chemical class 0.000 abstract description 2
- 235000005985 organic acids Nutrition 0.000 abstract description 2
- 238000006386 neutralization reaction Methods 0.000 abstract 1
- 229910000028 potassium bicarbonate Inorganic materials 0.000 abstract 1
- 235000015497 potassium bicarbonate Nutrition 0.000 abstract 1
- 239000011736 potassium bicarbonate Substances 0.000 abstract 1
- TYJJADVDDVDEDZ-UHFFFAOYSA-M potassium hydrogencarbonate Chemical compound [K+].OC([O-])=O TYJJADVDDVDEDZ-UHFFFAOYSA-M 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 15
- 235000018102 proteins Nutrition 0.000 description 12
- 239000000203 mixture Substances 0.000 description 8
- 239000003531 protein hydrolysate Substances 0.000 description 7
- 108010009736 Protein Hydrolysates Proteins 0.000 description 5
- 230000018109 developmental process Effects 0.000 description 5
- 230000004060 metabolic process Effects 0.000 description 5
- 235000015097 nutrients Nutrition 0.000 description 5
- 239000002253 acid Substances 0.000 description 4
- 235000013399 edible fruits Nutrition 0.000 description 4
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 3
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 235000001014 amino acid Nutrition 0.000 description 3
- 229940024606 amino acid Drugs 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 3
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 3
- 235000011130 ammonium sulphate Nutrition 0.000 description 3
- 239000011575 calcium Substances 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000004720 fertilization Effects 0.000 description 3
- 229910052748 manganese Inorganic materials 0.000 description 3
- 239000011572 manganese Substances 0.000 description 3
- 229910052698 phosphorus Inorganic materials 0.000 description 3
- 239000011574 phosphorus Substances 0.000 description 3
- 230000029553 photosynthesis Effects 0.000 description 3
- 238000010672 photosynthesis Methods 0.000 description 3
- 239000011591 potassium Substances 0.000 description 3
- 229910052700 potassium Inorganic materials 0.000 description 3
- 235000013311 vegetables Nutrition 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 239000004254 Ammonium phosphate Substances 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 239000003929 acidic solution Substances 0.000 description 2
- 150000007513 acids Chemical class 0.000 description 2
- 235000019289 ammonium phosphates Nutrition 0.000 description 2
- 230000003851 biochemical process Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 229930002875 chlorophyll Natural products 0.000 description 2
- 235000019804 chlorophyll Nutrition 0.000 description 2
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- -1 glycocol Chemical class 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 235000010755 mineral Nutrition 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 239000000049 pigment Substances 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Chemical group [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 2
- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 1
- ZMZGFLUUZLELNE-UHFFFAOYSA-N 2,3,5-triiodobenzoic acid Chemical compound OC(=O)C1=CC(I)=CC(I)=C1I ZMZGFLUUZLELNE-UHFFFAOYSA-N 0.000 description 1
- 239000004475 Arginine Substances 0.000 description 1
- 241000512260 Ascophyllum Species 0.000 description 1
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
- 101100283604 Caenorhabditis elegans pigk-1 gene Proteins 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 241000304829 Durvillaea Species 0.000 description 1
- 241001512723 Ecklonia Species 0.000 description 1
- 229930191978 Gibberellin Natural products 0.000 description 1
- 244000020551 Helianthus annuus Species 0.000 description 1
- 235000003222 Helianthus annuus Nutrition 0.000 description 1
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 1
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 1
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 1
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 1
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 1
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 1
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 1
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 1
- 241001466453 Laminaria Species 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 208000002720 Malnutrition Diseases 0.000 description 1
- 150000007945 N-acyl ureas Chemical class 0.000 description 1
- 108010089430 Phosphoproteins Proteins 0.000 description 1
- 102000007982 Phosphoproteins Human genes 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- 241000983742 Saccharina Species 0.000 description 1
- 241000195474 Sargassum Species 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 1
- 239000004473 Threonine Substances 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000009418 agronomic effect Effects 0.000 description 1
- 235000004279 alanine Nutrition 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 150000003863 ammonium salts Chemical class 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 229960001230 asparagine Drugs 0.000 description 1
- 235000009582 asparagine Nutrition 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000013522 chelant Substances 0.000 description 1
- 229910052729 chemical element Inorganic materials 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- WHDPTDWLEKQKKX-UHFFFAOYSA-N cobalt molybdenum Chemical compound [Co].[Co].[Mo] WHDPTDWLEKQKKX-UHFFFAOYSA-N 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 150000002169 ethanolamines Chemical class 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000005755 formation reaction Methods 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 description 1
- 239000003448 gibberellin Substances 0.000 description 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
- 239000003673 groundwater Substances 0.000 description 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 150000004679 hydroxides Chemical class 0.000 description 1
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 235000021073 macronutrients Nutrition 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 235000018343 nutrient deficiency Nutrition 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
- 230000008121 plant development Effects 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 230000001863 plant nutrition Effects 0.000 description 1
- 229920005862 polyol Polymers 0.000 description 1
- 150000003077 polyols Chemical class 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 235000011181 potassium carbonates Nutrition 0.000 description 1
- 235000011009 potassium phosphates Nutrition 0.000 description 1
- MSXHSNHNTORCAW-MPGIDXPLSA-M sodium;(3s,4s,5s,6r)-3,4,5,6-tetrahydroxyoxane-2-carboxylate Chemical compound [Na+].O[C@@H]1OC(C([O-])=O)[C@@H](O)[C@H](O)[C@@H]1O MSXHSNHNTORCAW-MPGIDXPLSA-M 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 230000014793 stomatal movement Effects 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- 239000004474 valine Substances 0.000 description 1
- 230000008511 vegetative development Effects 0.000 description 1
- 230000009105 vegetative growth Effects 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
- 235000010374 vitamin B1 Nutrition 0.000 description 1
- 239000011691 vitamin B1 Substances 0.000 description 1
- 238000009369 viticulture Methods 0.000 description 1
Abstract
Description
OFICIUL DE STAT PENTRU INVENȚII Șl MĂRCI Cerere de brevet de InvențieSTATE OFFICE FOR INVENTIONS AND TRADEMARKS Application for Invention patent
Nr...............2^......,^...4.LLNo...............2^......,^...4.LL
...........ii ϋ................ii ϋ.....
PRODUS BIOSTIMULANT PE BAZĂ DE SUBSTANȚE PROTEICE, PROCEDEU DE OBȚINERE Șl METODĂ DE APLICAREBIOSTIMULANT PRODUCT BASED ON PROTEIN SUBSTANCES, PROCESS OF OBTAINING AND METHOD OF APPLICATION
Invenția se referă la un produs biostimulant complex cu aplicare extraradiculară, reprezentând un concentrat de substanțe minerale, conținând azot, fosfor, potasiu, și substanțe organice cu proprietăți biostimulatoare provenite din surse proteice de origine animal prin hidroliză acidă, cu adaos de elemente secundate și microelemente fier, zinc, cupru, mangan, bor, cobalt molibden, magneziu, sulf, la un procedeu de obținere și la o metodă de aplicare a acestuia.The invention refers to a complex biostimulant product with extraradicular application, representing a concentrate of mineral substances, containing nitrogen, phosphorus, potassium, and organic substances with biostimulant properties derived from protein sources of animal origin through acid hydrolysis, with the addition of secondary elements and microelements iron, zinc, copper, manganese, boron, cobalt molybdenum, magnesium, sulphur, to a method of obtaining it and to a method of its application.
Se cunosc fertilizanți extraradiculari cu azot, fosfor, potasiu și microelemente care pot să conțină și substanțe organice de sinteză, extracte din plante sau hidrolizate proteice introduse cu scopul de a stimula metabolizarea substanțelor nutritive și a înlesni absorbția și pătrunderea în frunze a speciilor ionice sau moleculelor (RO 116082, RO 108953, RO 113846, RO 116082, RO 120258, RO 120403, RO 111005, RO 113846, RO 121814, RO 122197, RO 123139, RO 123140, RO 121814, RO 126939, RO 127400).Extra-root fertilizers with nitrogen, phosphorus, potassium and microelements are known which may also contain synthetic organic substances, plant extracts or protein hydrolysates introduced with the aim of stimulating the metabolism of nutrients and facilitating the absorption and penetration of ionic species or molecules into the leaves (RO 116082, RO 108953, RO 113846, RO 116082, RO 120258, RO 120403, RO 111005, RO 113846, RO 121814, RO 122197, RO 123139, RO 123140, RO 121814, RO 1269 39, RO 127400).
Se cunosc o serie de procedee de obținere a fertilizanților extraradiculari care constau în obținerea unei soluții de fosfați primari și / sau secundari de amoniu, amoniu și potasiu, adaos de azot sub forma de uree, săruri de amoniu, acizi organici policarboxilici, polioli, etanolamine, glucide (RO 108953, RO 113846, RO 116082, RO 118953), respectiv de înnobilare cu microelemente Fe, Mn, Cu, Zn, Mg, Co, Mo a unor hidrolizate proteice de natură animală sau vegetală (RO 103651, RO 116083, RO 120403, RO 127400, RO 126939).A series of procedures for obtaining extraradicular fertilizers are known, which consist in obtaining a solution of primary and / or secondary ammonium, ammonium and potassium phosphates, addition of nitrogen in the form of urea, ammonium salts, polycarboxylic organic acids, polyols, ethanolamines , carbohydrates (RO 108953, RO 113846, RO 116082, RO 118953), respectively for ennobling with microelements Fe, Mn, Cu, Zn, Mg, Co, Mo of protein hydrolysates of animal or vegetable nature (RO 103651, RO 116083, RO 120403, RO 127400, RO 126939).
Prezența în componența produselor fertilizante cu aplicare extraradiculară a substanțelor organice naturale sau de sinteză cu activitate de stimulare a creșterii, ca de exemplu heteroauxina, acidul betaindoilacetic, acidul triiodbenzoic, giberelina și derivații săi, aminoacizi precum glicocol, alanina, fenilalanina, prolina, oxiprolina, asparagina, glutamina, arginina, histidina, lizina, serina, treonina, valina, peptide, ureide și fosfoproteine în concentrații reduse chiar de zeci de ppm-uri conduc la creșterea suprafeței foliare a plantelor, a procesului de fotosinteza și a pigmenților clorofilieni, a dezvoltarea noilor formațiuni vegetale, a florilor și a fructelor.The presence in the composition of fertilizing products with extraradicular application of natural or synthetic organic substances with growth-stimulating activity, such as heteroauxin, betaindoylacetic acid, triiodobenzoic acid, gibberellin and its derivatives, amino acids such as glycocol, alanine, phenylalanine, proline, oxyproline, asparagine, glutamine, arginine, histidine, lysine, serine, threonine, valine, peptides, ureides and phosphoproteins in low concentrations of even tens of ppm lead to the increase of the leaf surface of plants, the process of photosynthesis and chlorophyll pigments, the development new plant formations, flowers and fruits.
Asimilarea substanțelor nutritive de către plante are loc într-un sistem complex de factori a cărui desfășurare este însoțită de efecte energointensive și se intercondiționează cu procesele metabolice din plantă și factorii externi pedoclimatici. Cum majoritatea proceselor metabolice se produc în plantă la nivel celular, în special în frunze, este evidentă importanța modalității de fertilizare extraradiculară, factor important care intervine semnificativ în creșterea și dezvoltarea plantelor, influența fiind decisivă și determinată de asigurarea necesarului de elemente nutritive sub o formă accesibilă plantelor. Elementele nutritive sunt absorbite sub formă de molecule neutre în cazul CO(NH2)2, ioni NOa’, K+, Ca+2, Mg+2, HPCU2', NH4+, hidroxizi, chelați, complecși organici cu aminoacizi, acizi organici, substanțe humice, extracte organice. în procesele de biosinteză participă elemente chimice, precum Fe, Cu, Mn, Mg, Zn, Co, Mo ș.a. sub formă ionică, unele din ele intrând în alcătuirea unor enzime ce catalizează diferite procese biochimice în timp ce altele asigură menținerea stării de echilibru necesară desfășurării proceselor biochimice, precum K+, Ca+2, CI-, la transportul și depozitarea substanțelor de acumulare, procesele de evapotranspirație K+, HPO42', sau azotul care în intră în constituția aminoacizilor, proteinelor protoplasmatice structurale, acizilor nucleici ADN, ARN, substanțelor cu rol bioactiv, pigmenților clorofilieni, a unor vitamine B1, B2, B6, B12, PP, enzime, a unor substanțe specifice din categoria alcaloizilor și în același timp participă la procesele de creștere.The assimilation of nutrients by plants takes place in a complex system of factors, the development of which is accompanied by energy-intensive effects and is interconnected with the metabolic processes in the plant and external pedoclimatic factors. As most of the metabolic processes occur in the plant at the cellular level, especially in the leaves, the importance of the method of extraradicular fertilization is obvious, an important factor that significantly intervenes in the growth and development of plants, the influence being decisive and determined by ensuring the necessary nutritional elements in a form accessible to plants. Nutrient elements are absorbed in the form of neutral molecules in the case of CO(NH 2 )2, NOa', K + , Ca +2 , Mg +2 , HPCU 2 ', NH4 + ions, hydroxides, chelates, organic complexes with amino acids, acids organics, humic substances, organic extracts. chemical elements, such as Fe, Cu, Mn, Mg, Zn, Co, Mo and others, participate in biosynthesis processes. in ionic form, some of them entering into the composition of enzymes that catalyze various biochemical processes while others ensure the maintenance of the equilibrium state necessary for the development of biochemical processes, such as K + , Ca +2 , CI - , for the transport and storage of accumulation substances, the processes of evapotranspiration K + , HPO4 2 ', or the nitrogen that enters into the constitution of amino acids, structural protoplasmic proteins, nucleic acids DNA, RNA, substances with a bioactive role, chlorophyll pigments, some vitamins B1, B2, B6, B12, PP, enzymes, of specific substances from the category of alkaloids and at the same time participate in growth processes.
Compoziția fertilizanților cu aplicare extraradiculară trebuie selectată astfel încât să furnizeze conținutul de nutrienți necesari pentru a contracara situații de stres determinate de cererea de nutrienți în timpul creșterii plantelor, condiților nefavorabile de temperature, umiditate, tehnologie sau diferitelor tipuri de soluri.The composition of fertilizers with extraradial application must be selected in such a way as to provide the content of nutrients necessary to counteract stress situations determined by the demand for nutrients during plant growth, unfavorable conditions of temperature, humidity, technology or different types of soils.
In categoria produselor biostimulante se pot încadra hidrolizatele proteice de origine animala sau vegetală, extractele din alge (Ascophyllum, Durvillaea, Ecklonia, Laminaria/Saccharina, Sargassum) și substanțele humice.The category of biostimulant products can include protein hydrolysates of animal or vegetable origin, algae extracts (Ascophyllum, Durvillaea, Ecklonia, Laminaria/Saccharina, Sargassum) and humic substances.
Se cunoaște faptul că hidrolizatele proteice reprezintă medii polidisperse formate din polipeptide, peptide, oligopeptide și amnioacizi liberi, într-un procent determinat de gradul de hidroliză obținut în proces și că acestea au capacitatea de a chelata o serie de cationic metalici precum Fe, Mn, Cu, Zn, Mg, Ca, Co, fapt ce le conferă o gamă largă de aplicații în industria farmaceutica si cosmetica, industria chimică, precum si cea a fertilizanților si a produselor alimentare (US 4,427,658, US 4,169,716, US 4,491,464, US 7,271,128 B2, US 2005-0086987 A1, US 2007-0087039 A1).It is known that protein hydrolysates represent polydisperse media made up of polypeptides, peptides, oligopeptides and free amniotic acids, in a percentage determined by the degree of hydrolysis obtained in the process, and that they have the ability to chelate a series of metal cations such as Fe, Mn, Cu, Zn, Mg, Ca, Co, which gives them a wide range of applications in the pharmaceutical and cosmetic industry, the chemical industry, as well as that of fertilizers and food products (US 4,427,658, US 4,169,716, US 4,491,464, US 7,271,128 B2 , US 2005-0086987 A1, US 2007-0087039 A1).
Hidrolizatele proteice au câștigat importanță ca biostimulatori datorită spectru larg de acțiuni pozitive asupra plantelor, influiențând pe lângă creșterea și dezvoltarea plantelor, procesele de fotosinteză, pe cele biochimice și fenomenele biologice, precum germinarea, pornirea mugurilor, tubercurilor și bulbilor, circulația apei, a sevei brute și elaborate, evapotranspirația, absorția și transportul substanțelor anorganice și organice, mișcarea stomatelor, rezistenta la cădere a frunzelor, florilor si fructelor, înflorirea si fecundarea, rezistența plantelor în condiții de stress pedoclimatic, tehnologic și agenții patogeni, concomitant cu reducerea dozelor aplicate de îngrășăminte chimice de sinteză.Protein hydrolysates have gained importance as biostimulators due to a wide spectrum of positive actions on plants, influencing, in addition to plant growth and development, photosynthesis processes, biochemical ones and biological phenomena, such as germination, initiation of buds, tubers and bulbs, circulation of water, sap raw and elaborated, evapotranspiration, absorption and transport of inorganic and organic substances, stomatal movement, resistance to falling of leaves, flowers and fruits, flowering and fertilization, resistance of plants under conditions of pedoclimatic, technological stress and pathogens, concomitant with the reduction of applied doses of synthetic chemical fertilizers.
Problema tehnică pe care o rezolvă invenția constă în obținerea unor compoziții biostimulante complexe de înaltă eficiență și eficacitate agronomică, cu efecte și de protecție a mediului, prin valorificarea superioara folosind procesul de hidroliză acida a unor substanțe proteice naturale, intr-o matrice de tip NPK prin inobilarea cu săruri minerale și o concentrație mare de elemente secundare și microelemente complet chelatate, compoziție fertilizantă care optimizează nutriția plantelor, favorizează absorbția și metabolizarea în parenchimul frunzei a ionilor, stimulează dezvoltarea vegetativă radiculară și extraradiculară, crește rezistența plantelor la factorii de stres climatic și tehnologic, la agenții patogeni.The technical problem that the invention solves consists in obtaining complex biostimulant compositions of high efficiency and agronomic effectiveness, with environmental protection effects, through the superior valorization using the process of acid hydrolysis of natural protein substances, in an NPK type matrix by ennobling with mineral salts and a high concentration of fully chelated secondary elements and microelements, fertilizing composition that optimizes plant nutrition, favors absorption and metabolism in the leaf parenchyma of ions, stimulates root and extra-root vegetative development, increases plant resistance to climatic stress factors and technologically, to pathogenic agents.
Compoziția biostimulantă complexă ce face obiectul invenției are un raport al macronutrienților N : P2O5 : K2O cuprins între 4: 1: 1,5 și 9: 1: 3 îndeplinind condițiile de aplicare extraradiculară în diferite faze de vegetație ale plantelor, asigurând atât creșterea vegetativă, cât și prevenirea și combaterea carențelor nutriționale. Biostimulantul asigură o foarte buna aderență a elementelor nutritive pe frunze, inclusiv un transfer ridicat al acestora in metabolismul plantelor și o acoperire eficientă a suprafeței foliare.The complex biostimulant composition that is the subject of the invention has a ratio of macronutrients N : P2O5 : K2O between 4: 1: 1.5 and 9: 1: 3 meeting the conditions of extraradicular application in different vegetation phases of the plants, ensuring both vegetative growth, as well as preventing and combating nutritional deficiencies. The biostimulant ensures a very good adhesion of the nutritional elements on the leaves, including a high transfer of them in plant metabolism and an effective coverage of the leaf surface.
Aplicarea extraradiculară a biostimulantului în trei tratamente poate reduce cu 10...15% doza de îngrășăminte clasice cu azot utilizată în fertilizarea de bază, factor important în asigurarea protecției mediului prin reducerea fenomenelor de livigare în apa freatică a azotului.The extraradicular application of the biostimulant in three treatments can reduce by 10...15% the dose of classic nitrogen fertilizers used in basic fertilization, an important factor in ensuring environmental protection by reducing nitrogen leaching into the groundwater.
Produsul biostimulant complex cu aplicare extraradiculară, conform invenției, este constituit din: 7,4...9,8% azot total, din care 3,2...4,6% azot amoniacal, 3,3...3,6% azot nitric, 0,9...1,6% azot organic, 0,8...2,4% pentaoxid de fosfor, 2,7...3,4% oxid de potasiu, 7...11,4% substanțe organice din care 5,3...9,2% ca substanțe proteice, respectiv peptide, oligopeptide și amnioacizi liberii, 0,09...0,1% fier, 0,07...0,1% zinc, 0,06...0,1% cupru, 0,03...0,04% bor, 0,05...0,08% mangan, 0,02...0,06% magneziu, 0,006...0,02% molibden, 0,001...0,003% cobalt, 0,5...1,6% sulf ca SO3, un raport molar N:P2Os:K2O cuprins între 40 : 1: 2 și 90 : 1 : 5 și un pH = 5,5...6,5.The complex biostimulant product with extraradicular application, according to the invention, consists of: 7.4...9.8% total nitrogen, of which 3.2...4.6% ammoniacal nitrogen, 3.3...3, 6% nitric nitrogen, 0.9...1.6% organic nitrogen, 0.8...2.4% phosphorus pentaoxide, 2.7...3.4% potassium oxide, 7... 11.4% organic substances of which 5.3...9.2% as protein substances, respectively peptides, oligopeptides and free amnioacids, 0.09...0.1% iron, 0.07...0, 1% zinc, 0.06...0.1% copper, 0.03...0.04% boron, 0.05...0.08% manganese, 0.02...0.06% magnesium, 0.006...0.02% molybdenum, 0.001...0.003% cobalt, 0.5...1.6% sulfur as SO3, a N:P2Os:K2O molar ratio between 40:1:2 and 90 : 1 : 5 and a pH = 5.5...6.5.
Procedeul de obținere a produsului biostimulant, conform invenției, constă în reacția de hidroliză acidă în prezență de 600...660g HNO3 de concentrație 28...30% și 15...45g H3PO4 de concentrație 85%, la un raport molar acid azotic : acid fosforic de 30...11.5 : 1, a 100...180 g clei de oase, ca sursă de substanțe proteice, la temperatura de 8O...85°C, timp de 2...4 ore, racirea soluției la temperature de 2O...25°C, neutralizarea acesteia pana la un pH de 5,5...6,5 cu 180...250g apa amoniacala de concentrație 23% și 45...60g K2CO3 si adăugarea pentru 1000g de produs a 2,4...6,5 g sare tetrasodică a acidului etilendiaminotetraacetic, 1...2 g CeHsCh, 1,5...3 g MgSO4 7H2O, 7,8...9,3g FeEDTANa2, 5,1...6,1g ZnEDTANa2, 4,3...5,2g CuEDTANa2, 4,5...5,0g MnEDTANa2, 0,05...0,15g Οο(ΝΟ3)2 6H2O, 1...4g Na2B4O7 WH2O, 0,12...0,4g (ΝΗ4)6Μθ7θ24 4H2O si 10...30g (NH4)2SO4 și agitarea timp de o oră la temperatura de 2O...22°C.The process of obtaining the biostimulant product, according to the invention, consists in the acid hydrolysis reaction in the presence of 600...660g of HNO3 of 28...30% concentration and 15...45g of H3PO4 of 85% concentration, at an acidic molar ratio nitric: phosphoric acid of 30...11.5:1, a 100...180 g of bone glue, as a source of protein substances, at a temperature of 8O...85°C, for 2...4 hours, cooling the solution to a temperature of 2O...25°C, neutralizing it to a pH of 5.5...6.5 with 180...250g ammoniacal water of 23% concentration and 45...60g K2CO3 and adding for 1000g of product, 2.4...6.5 g tetrasodium salt of ethylenediaminetetraacetic acid, 1...2 g CeHsCh, 1.5...3 g MgSO 4 7H 2 O, 7.8...9 ,3g FeEDTANa 2 , 5.1...6.1g ZnEDTANa 2 , 4.3...5.2g CuEDTANa 2 , 4.5...5.0g MnEDTANa 2 , 0.05...0.15g Οο(ΝΟ 3 ) 2 6H2O, 1...4g Na 2 B 4 O7 WH2O, 0.12...0.4g (ΝΗ4) 6 Μθ7θ 2 4 4H2O and 10...30g (NH4)2SO4 and stirring time for one hour at a temperature of 2O...22°C.
Metoda de aplicare a fertilizantului extraradicular, conform invenției, constă în aceea că produsul se administrează prin pulverizare pe plante sub formă de soluție apoasă de concentrație 0,25...2,5%, în cantitate de 150...1500 l/ha în funcție de cultura și fazele de vegetație ale plantelor, respective aplicat pe sămânță premergător semănatului ca soluție de concentrație 0,15 - 0,3% biostimulant, în cantitate de 10...15 litri la tona de semințe.The method of applying the extraradicular fertilizer, according to the invention, consists in the fact that the product is administered by spraying on plants in the form of an aqueous solution with a concentration of 0.25...2.5%, in an amount of 150...1500 l/ha depending on the crop and vegetation phases of the plants, respectively applied to the seed before sowing as a 0.15 - 0.3% biostimulant concentration solution, in the amount of 10...15 liters per ton of seeds.
Produsul biostimulant aplicat extraradicular asigură sporuri de producție de 10...15% la culturile de floarea-soarelui, grâu si porumb, 20...25% în legumicultură, pomicultură și viticultură, favorizează acumularea elementelor azot, fosfor și potasiu în plante și fructe, crește cu 15...20% procesul de fotosinteză în frunze, asigură dezvoltarea sistemului foliar și radicular, precum si crește rezistența plantelor la factorii de stres pedoclimatic.The biostimulant product applied extraradially ensures production increases of 10...15% in sunflower, wheat and corn crops, 20...25% in vegetable, fruit and viticulture, favors the accumulation of nitrogen, phosphorus and potassium elements in plants and fruits, increases by 15...20% the photosynthesis process in the leaves, ensures the development of the leaf and root system, as well as increases the plants' resistance to pedoclimatic stress factors.
Se dau în continuare 2 exemple de realizare a invenției.2 examples of the invention are given next.
Exemplull:Example:
Pentru obținerea a 1000 g de produs biostimulant, într-o suluție acida ce conține 600g acid HNO3 de concentrație 28% și 15 g H3PO4 de concentrație 85% se adaugă treptat și sub agitare 100 g clei de oase, ca sursă de substanțe proteice. După omogenizarea amestecului, acesta se încălzește la 8O...85°C și se hidrolizează substanțele organice timp de doua ore la temperatură constantă, sub agitare continuă.To obtain 1000 g of biostimulant product, in an acidic solution containing 600 g of HNO3 acid of 28% concentration and 15 g of H3PO4 of 85% concentration, 100 g of bone glue is added gradually and with stirring, as a source of protein substances. After the mixture is homogenized, it is heated to 80...85°C and the organic substances are hydrolyzed for two hours at a constant temperature, under continuous stirring.
Soluția rezultată se răcește la 2O...22°C si se neutralizează treptat aciditatea liberă, sub agitare si răcire la temperatură constantă, cu 180g apă amoniacală de concentrație 23% si 45 g K2CO3, până la un pH cuprins între 5,5...6,0.The resulting solution is cooled to 2O...22°C and the free acidity is gradually neutralized, under stirring and cooling at a constant temperature, with 180g of ammonia water of 23% concentration and 45g of K2CO3, until a pH between 5.5. ..6.0.
în 100 cm3 apă demineralizată se prepară soluția de elemente secundare si microelemente prin dizolvarea sub agitare a: 2,4g sare tetrasodică a acidului etilendiaminotetraacetic, 1g CeHsCh, 1,5g MgSCU 7H2O, 7,8g FeEDTANa2, 5,1gin 100 cm 3 of demineralized water, prepare the solution of secondary elements and trace elements by dissolving under stirring: 2.4g tetrasodium salt of ethylenediaminetetraacetic acid, 1g CeHsCh, 1.5g MgSCU 7H2O, 7.8g FeEDTANa2, 5.1g
ZnEDTANaz, 4,3g CuEDTANa2, 4,5g MnEDTANa2, 0,05g Co(NO3)2 6H2O, 1g Na2B4O? IOH2O, 0,12g (NH4)6Mo?O24 4H2O si 10g (NH4)2SO4.ZnEDTANaz, 4.3g CuEDTANa2, 4.5g MnEDTANa2, 0.05g Co(NO3)2 6H2O, 1g Na2B4O? IOH2O, 0.12g (NH4)6Mo?O24 4H2O and 10g (NH4)2SO4.
Soluția de elemente secundare si microelemente se adaugă treptat peste soluția de hidrolizatul proteic obținută anterior și se continuuă agitarea, timp de o oră la temperatura de 2O...22°C.The solution of secondary elements and microelements is gradually added over the previously obtained protein hydrolyzate solution and the stirring is continued for one hour at a temperature of 20...22°C.
Biostimulantul complex, cu aplicare extraradiculara, pe bază de substanțe proteice naturale, conform invenției, este constituit din: 7,4% azot total, din care 3,2% azot amoniacal, 3,3% azot nitric, 0,9% azot organic, 0,8% pentaoxid de fosfor, 2,7% oxid de potasiu, 7% substanțe organice din care 5,3% ca substanțe proteice, respectiv peptide, oligopeptide și amnioacizi liberii, 0,09% fier, 0,07% zinc, 0,06% cupru, 0,03% bor, 0,05% mangan, 0,02% magneziu, 0,006% molibden, 0,001% cobalt, 0,5% sulf ca SO3 și un pH = 5,5...6,0.The complex biostimulant, with extraradicular application, based on natural protein substances, according to the invention, consists of: 7.4% total nitrogen, of which 3.2% ammoniacal nitrogen, 3.3% nitric nitrogen, 0.9% organic nitrogen , 0.8% phosphorus pentaoxide, 2.7% potassium oxide, 7% organic substances of which 5.3% as protein substances, namely peptides, oligopeptides and free amnioacids, 0.09% iron, 0.07% zinc , 0.06% copper, 0.03% boron, 0.05% manganese, 0.02% magnesium, 0.006% molybdenum, 0.001% cobalt, 0.5% sulfur as SO3 and a pH = 5.5... 6.0.
Exemplul 2:Example 2:
Pentru obținerea a 1000g de produs biostimulant, într-o suluție acida ce conține 660g acid azotic de concentrație 30% și 45 g acid fosforic de concentrație 85% se adaugă treptat și sub agitare 180g clei de oase, ca sursă de substanțe proteice. După omogenizarea amestecului, acesta se încălzește la 8O...85°C și se hidrolizează substanțele organice timp de patru ore la temperatură constantă, sub agitare continuă.To obtain 1000g of biostimulant product, in an acidic solution containing 660g of nitric acid of 30% concentration and 45g of phosphoric acid of 85% concentration, 180g of bone glue is added gradually and with stirring, as a source of protein substances. After the mixture is homogenized, it is heated to 80...85°C and the organic substances are hydrolyzed for four hours at constant temperature, under continuous stirring.
Soluția rezultată se răcește la 2O...22°C si se neutralizează treptat aciditatea, sub agitare si răcire la temperatură constantă, cu 250g apa amoniacala de concentrație 23% si 60g carbonat de potasiu până la un pH cuprins între 6,0...6,5.The resulting solution is cooled to 2O...22°C and the acidity is gradually neutralized, with stirring and cooling at a constant temperature, with 250g of 23% ammonia water and 60g of potassium carbonate until a pH between 6.0.. .6.5.
în 100 cm3 apă demineralizată se prepară soluția de elemente secundare si microelemente prin dizolvarea sub agitare a: 8,5g sare tetrasodică a acidului etilendiaminotetraacetic, 3g acid citric, 4g MgSO4 7H2O, 10,8g FeEDTANa2, 7,6g ZnEDTANa2, 6,8g CuEDTANa2, 7,5g MnEDTANa2, 0,15g Co(NO3)2 6H2O, 4g Na2B40y IOH2O, 0,4g (ΝΗ4)6Μθ7θ24 4H2O si 30g (NH4)2SO4.in 100 cm 3 of demineralized water, the solution of secondary elements and microelements is prepared by dissolving under stirring: 8.5g tetrasodium salt of ethylenediaminetetraacetic acid, 3g citric acid, 4g MgSO4 7H2O, 10.8g FeEDTANa2, 7.6g ZnEDTANa2, 6.8g CuEDTANa2, 7.5g MnEDTANa2, 0.15g Co(NO3)2 6H2O, 4g Na2B40y IOH2O, 0.4g (ΝΗ 4 )6Μθ7θ 2 4 4H2O and 30g (NH4)2SO4.
Soluția de elemente secundare si microelemente se adaugă treptat peste soluția de hidrolizatul proteic obținută anterior și se continuuă agitarea, timp de o oră la temperatura de 2O...22°C.The solution of secondary elements and microelements is gradually added over the previously obtained protein hydrolyzate solution and the stirring is continued for one hour at a temperature of 20...22°C.
Biostimulantul complex, cu aplicare extraradiculara, pe bază de substanțe proteice naturale, conform invenției, este constituit din: 9,8% azot total, din care 4,6% azot amoniacal, 3,6% azot nitric, 1,6% azot organic, 2,4% pentaoxid de fosfor, 3,4% oxid de potasiu, 11,4% substanțe organice din care 9,2% ca substanțe proteice, respectiv peptide, oligopeptide și amnioacizi liberi, 0,1% fier, 0,1% zinc, 0,1% cupru, 0,04% bor, 0,08% mangan, 0,06% magneziu, 0,02% molibden, 0,003% cobalt, 1,6% sulf ca SO3 și un pH = 6...6,5.The complex biostimulant, with extraradicular application, based on natural protein substances, according to the invention, consists of: 9.8% total nitrogen, of which 4.6% ammoniacal nitrogen, 3.6% nitric nitrogen, 1.6% organic nitrogen , 2.4% phosphorus pentaoxide, 3.4% potassium oxide, 11.4% organic substances of which 9.2% as protein substances, respectively peptides, oligopeptides and free amnioacids, 0.1% iron, 0.1 % zinc, 0.1% copper, 0.04% boron, 0.08% manganese, 0.06% magnesium, 0.02% molybdenum, 0.003% cobalt, 1.6% sulfur as SO3 and a pH = 6. ..6.5.
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