RO116205B1 - Process for preparing an active product for regulating intestinal flora in humans - Google Patents

Process for preparing an active product for regulating intestinal flora in humans

Info

Publication number
RO116205B1
RO116205B1 RO96-01321A RO9601321A RO116205B1 RO 116205 B1 RO116205 B1 RO 116205B1 RO 9601321 A RO9601321 A RO 9601321A RO 116205 B1 RO116205 B1 RO 116205B1
Authority
RO
Romania
Prior art keywords
humans
preparing
intestinal flora
active product
hours
Prior art date
Application number
RO96-01321A
Other languages
Romanian (ro)
Inventor
Tudora Caraiani
Maria Pamfil
Doina Dobrovolschi
Sanda Stan
Original Assignee
Institutul Naţional De Cercetare-Dezvoltare Chimico-Farmaceutică - I.C.C.F. Bucureşti R.A.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institutul Naţional De Cercetare-Dezvoltare Chimico-Farmaceutică - I.C.C.F. Bucureşti R.A. filed Critical Institutul Naţional De Cercetare-Dezvoltare Chimico-Farmaceutică - I.C.C.F. Bucureşti R.A.
Priority to RO96-01321A priority Critical patent/RO116205B1/en
Publication of RO116205B1 publication Critical patent/RO116205B1/en

Links

Abstract

The invention relates to a process for preparing an active product regulating the intestinal flora in humans by biosynthesis, by means of the microorganism Bacillus subtilis, said process comprising the following stages: the inoculum production by growing the strain I.C.C.F.-77A in a medium containing corn extract and mineral salts, at 30...32°C for 18...20 hours, the bioprocess as such by vegetative growth in an identical medium and under the same conditions, with maximal sporulation of the bacterial population in a ratio of more than 90% for 22...26 hours, settling and centrifuging at 3,000 rpm for 20...30 min while cooling, drying the sporulated biomass by lyophilization, finally producing a powder with 1.7x10 10...2x10 12 spores/gramme, raw protein with 8...15% dry substance and free amino acids: aspartic acid, valine, leucine, gluconic acid, alpha-alanine and heavy metals 0.001%.
RO96-01321A 1996-06-28 1996-06-28 Process for preparing an active product for regulating intestinal flora in humans RO116205B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RO96-01321A RO116205B1 (en) 1996-06-28 1996-06-28 Process for preparing an active product for regulating intestinal flora in humans

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RO96-01321A RO116205B1 (en) 1996-06-28 1996-06-28 Process for preparing an active product for regulating intestinal flora in humans

Publications (1)

Publication Number Publication Date
RO116205B1 true RO116205B1 (en) 2000-11-30

Family

ID=64358088

Family Applications (1)

Application Number Title Priority Date Filing Date
RO96-01321A RO116205B1 (en) 1996-06-28 1996-06-28 Process for preparing an active product for regulating intestinal flora in humans

Country Status (1)

Country Link
RO (1) RO116205B1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11718892B2 (en) 2015-03-30 2023-08-08 CvPlus GmbH Biological ore processing for the isolation of heavy metals

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11718892B2 (en) 2015-03-30 2023-08-08 CvPlus GmbH Biological ore processing for the isolation of heavy metals

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