RO114943B1 - Process and apparatus for preparing medicine-solutions and method for cellular treatments using said solutions - Google Patents

Abstract

The invention relates to an apparatus and a process for preparing medicine-solutions and to a method for cellular treatments using said solutions. According to the invention, ionic solutions specific to each disease are obtained by employing the claimed apparatus and process, while the method of treatment is applied to cellular diseases with a view to detoxicating the organism, concomitantly with revitalizing and energizing the body cells, being directly applicable to treating the diseases of the digestive, osseous, neuro-muscular, blood-vascular, urinary, glandular systems, of the blood, as well as to treating external diseases.

Description

RO 114943 Β1

The invention relates to an apparatus and a process for obtaining medicinal solutions and to a method of cellular treatments using these medicinal solutions. Using the device and the process according to the invention, ionic solutions specific to each disease are obtained, and the treatment method is applied to cellular diseases, in order to detoxify the body, in parallel with the revitalization and energization of the body's cells, with direct applicability to the treatment of diseases of the digestive system, of the bone system , the neuromuscular system, the circulatory system, the urinary system, the glandular system, the blood, as well as the treatment of external conditions.

A US patent 4,663,166 discloses in vivo techniques and compositions for restoring fluid electrolytes and nutrients in the regulation of metabolic processes in living mammals.

This invention presents therapies with water and electrolytes, simultaneously with the normalization of blood composition, by parenteral, intra-arterial, intramuscular, intravascular, dialysis or oral introduction into the body of aqueous solutions in which:

- the ratio between the milliequivalents per liter of sodium cations and the milliequivalents per liter of chlorine anions is so selected as to have values that are normally found in the blood;

- to find in the blood at least one couple selected to be close to balance from a group consisting of:

a. bicarbonate and carbon dioxide

b. l-lactate' and pyruvate'

c. d-betahydroxybutyraf and acetoacetate'

- to maintain the pH within the limits of 5 to 9.

The electrolyte solutions used can be included in two distinct classes:

- class I, which includes fluids containing at least one, but no more than two metal cations selected from the group comprising sodium, potassium, calcium and magnesium;

- class II which includes solutions containing at least three, but generally not more than four metal cations from the same group as those in class I.

Class I solutions are administered in doses not greater than 1 liter in 24 h for adults, the typical dose being 500 ml/day. Class II solutions are administered in doses chosen by the doctor, between 0 and 100 I/24 h for an adult, depending on the circumstances.

The electrolytic solutions proposed in this patent cannot be used in the case of hemodialysis, because the organs affected in these cases are the kidneys and the liver and, depending on the chronicity of the condition, the circulatory system and the spleen. The blood must be protected from an overload in the elements mentioned in the patent, which lead to damage to the kidney tissue and the other organs mentioned. The method in the patent cannot be used either as an adjuvant in hemodialysis or as a substitute for hemodialysis.

Patent DE 3321831 presents a device that allows the local influence or destruction of liver cells, in particular, neoplastic ones, with the aim of evaluating the stage of healing using electrolytic means. An electrical contact with the extracellular and intracellular substrates of the tumor, used as the main electrolyte, is made through an auxiliary electrolyte liquid, in separate compartments of the device that are provided with non-conductive cannulas, without exposing the healthy tissue to current. The electrodeposition of the toxic substances decomposed from the tumor takes place in a harmless way for the patient, at the electrodes in the separate compartments, located outside the body.

The disadvantage of using the device is that, through the application of the electric field, neoplastic cells from the tumor on which it is acting can be disseminated in the body, through their direct energetic charge.

An electrolysis cell for wastewater treatment is presented in patent WO 91/18837. The cell comprises several anodes, in a titanium-plated grid, interspersed between the cathodes.

RO 114943 Β1

Anodes are contained in compartments of a materia! ion-permeable microporous, which serves as a diaphragm. The acolyte circulation includes a siphon to control the top of the acolyte so as to avoid unwanted mixing of anolyte and catholyte.

The electrolysis cell in this patent performs an industrial, impure electrolysis, one of the electrodes being plated with Ti and not completely made of Ti.

The device according to the invention allows the creation of ionic solutions of quality and ionic concentration specific to the disease being treated. The device is an electrolyser consisting of a device for converting alternating current into direct current, two electrodes and a diaphragm. The ionic solutions are separated with the help of the diaphragm, which allows the light penetration of the ionic solutions and their separation during electrolysis.

The process according to the invention is based on the mixture in the electrolyzer of the stoichiometrically calculated elements and the electrolytically purified water, the electrolysis of this mixture, the separation of the ionic solution of the desired polarity, containing the purified elements and their activation through a new electrolysis.

The treatment method according to the invention uses ionic solutions-medicine, which contain elements specific to the diseases being treated and is based on the creation of ion channels in the body, which allow the components of the solutions to be fixed in the affected organ. The method contains two phases, in a first phase an ionic solution is used that acts to dislodge the diseased cells, simultaneously with the revitalization and energization of the affected area, and in a second phase other ionic solutions are used, which achieve total destruction of the agents that caused the condition.

The advantages of the invention are the following; 70

- non-toxic ion-drug solutions are used;

- they can be administered orally, parenterally, intramuscularly, intravenously and externally;

- the dislocation of cells from the affected organ is obtained, simultaneously with its revitalization and energization;

- the total destruction of the agents that caused the condition is obtained; 75

- in the case of external treatment, no scar remains;

- using the ionic-medicine solutions, the cellular treatment of the entire body is carried out, using pure and activated elements specific to the organs being treated.

Below is an example of an embodiment of the invention in connection with fig. 1, which represents the electrolysis apparatus. 80

The apparatus for obtaining ionic solutions according to the invention, comprises an electrolysis vessel 1, provided at the bottom with an outlet nozzle 6, vessel that closes with a lid 7. Two electrodes 2a and 2b and a diaphragm are fixed on the lower part of the lid 5.

A rectifier diode 3 fixed on the electrode 2a, outside the vessel 1, is connected to one wire of the power cord 4, the other wire being soldered to the electrode 2b.85

Electrodes 2a and 2b are made of Cr, Ni, Mo (18/8/3) type W4A stainless steel. in another constructive variant, electrode 2b can be made of Ti or Ag, and electrode 2a is made of the same W4A type steel. The ratio between the length and width of the electrodes is about 1/3, and their thickness is about 2 mm. The construction of the electrodes and the parameters of the electrolysis avoid the appearance of the ebullioscopic effect. 90

The diaphragm 5 is formed from a natural textile material, preferably cotton or natural silk. The natural silk fabric is made of 20 denx2 cabled thread.

The procedure for obtaining ionic solutions includes the following phases:

- the stoichiometric calculation of the components that make up the ionic solution to be prepared; 95

- qualitative analysis of the water used for electrolysis, determining the amount and types of impurities in the water;

RO 114943 Β1

- water purification by simple electrolysis, lasting between two and twelve minutes, depending on the hardness of the water;

- electronegatively charged water, purified by electrolysis, is decanted and further used for the preparation of ionic solutions;

- the amount of electronegatively charged water required is calculated;

- the electronegatively charged water and the stoichiometrically calculated components are mixed in the electrolyzer;

- the electrolysis vessel 1 is covered with the lid 7 on which the electrolyses 2a, 2b and the diaphragm 5 are fixed and the electrolysis is carried out, obtaining the solution with the pure elements of interest;

- the duration of the electrolysis is from two to twelve minutes, depending on the concentration of the components;

- evacuate the part of the solution that is not of interest in terms of purity and polarity;

- electrolysis is repeated on the solution containing pure elements in order to activate the respective elements.

Thus, ionic solutions are obtained that include elements of positive or negative polarity used in cell treatments using the method according to the invention.

Through the electrochemistry process carried out by electrolysis according to the invention, any element from the periodic system of elements can be extracted, purified and activated. Activation is done by increasing the energy level of the electrons in the last orbit, which provides the element used with maximum reactivity.

These elements extracted, purified and activated in ionic solutions are used in cellular treatments.

By determining the biological age of the human body, with the help of intracellular electrophoresis, the correlation between the number of electronegative and electroneutral nuclei in the body was found, thus establishing that the percentage of electronegative nuclei is the highest at a young age, then it decreases as the body progresses in age.

ionic solutions can be introduced into the body, the compounds of which penetrate the entire cellular mass of the body, creating ion channels and revitalizing the cells of the entire body. By creating ion channels, ion solutions with the necessary components to treat the affected organ dislodge the diseased cells, increase the phagocytosis process and heal the affected part of the diseased organ.

Through the method of cell treatments according to the invention, the creation of intracellular ion channels is ensured, through which the electronegativization of the cell nuclei is obtained, which leads to the revitalization and energization of the whole organism, thus being able to act directly on some diseases.

The treatment method is applied internally per os, parenterally, intramuscularly or intravenously and externally through plastering.

The method of cellular treatments according to the invention uses drugs prepared in the form of tonic solutions, made with the device described according to the invention, using the described process and is based on the creation in the body of ion channels of the appropriate polarity, which allow the fixing of the components of the ionic solutions-medicine in the organ affected.

in a first phase, in order to dislodge the diseased cells, simultaneously with the revitalization and energization of the affected area, an ionic solution is administered in doses and at predetermined time intervals, and in a second phase, the affected area is acted upon, aiming at the destruction total of the agents that caused the condition by using other ionic solutions, of composition and concentration corresponding to the disease being treated, the respective ionic solutions being calculated in ml/kbody and applied according to body weight and age.

RO 114943 Β1

The second phase of treatment is repeated if medical analyzes indicate the need for this.

Both the ionic solutions used in the first phase and those used in the second phase are usually 150 electronegative. Electropositive solutions are used in the case of digestive tubes with hypoacidity.

Tests were made regarding the toxicity of the drug-ionic solutions used. in 1993, at the Cantacuzino Institute, the lack of toxicity of the drug 1 A was confirmed by intraperitoneal injection in mice that were injected with 0.5 ml of solution and that were kept 155 under observation for seven days.

in February 1998 also at the Cantacuzino Institute, the following ionic drug solutions were tested: 1A, 2A, 3A, 1B, 2B, 3B on adult mice. 0.5 ml of each solution was administered, both by intraperitoneal injection and orally, in separate batches and the mice were followed for seven days, finding 100% survival and the absence of any 160 sign of disease .

The lack of toxicity of solution 2B, 90% concentration, was also verified by the Chemical-Pharmaceutical Research Institute (ICCF) in July 1999. The checks were made on groups of mice, by a) intraperitoneal injection of the solution into amount of 1 ml; b) by intravenous injection of the solution in the amount of 0.5 ml, on groups of rats by: a) intraperitoneal injection of 3 ml of solution; b) by intravenous injection of 1 ml of solution and on groups of rabbits by intravenous injection to the auricular vein of 3 ml of solution.

All groups were kept under observation for 14 days, after which one animal from each group was sacrificed and it was found that during the observation period all animals behaved normally, no sign of disease was reported, the internal organs (organs genitals, kidneys, 170 adrenal glands, liver, pancreas, spleen, heart, lungs, meninges, brain, stomach, intestines, flesh, inner skin), blood, had a normal appearance. Through these tests, tolerance to the injection was also ascertained.

The antimicrobial, antifungal, antiviral and antineoplastic activity of the drug solutions was also tested. 175

The antimicrobial activity was tested in several steps. in 1993, at the Cantacuzino Institute, solutions 1 A, 2A, 3A, 1B, 2B, 3B were tested on the following international reference strains: Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa, Staphylococcus aureus. Only solution 3A was found to be active and only undiluted.

in 1998, the antibacterial and bactericidal testing of solutions 1 B and 2B, with a concentration of 90%, was repeated on the following bacteria: staphylococcus aureus, Streptococcus faecalis, Proteus vulgaris, Salmonella typhi, Shigella flexneri, Escherichia coli, Klebsiella pneumonae, Pseudomonas aeruginosa, Bacillus subtilis. The testing was done by the diffusimetric method, by the method of incorporating the test substance into the culture medium and by the method of limited time contact between the test substance and the test microorganisms. Solutions 1B 185 and 2B were found to exhibit antimicrobial action manifested by inhibiting the development of test microorganisms. It was also found that the two solutions have bactericidal action, acting on both vegetative and sporulated forms. Of the nine types of bacteria tested, five types were completely destroyed after thirty minutes of contact, and the other four types, after the same time interval, were not completely destroyed, but 190 a very large reduction in the number of colonies was obtained . in each case testing was done on two batches of solutions 1B and 2B, finding that the initial results are reproducible.

Antifungal and fungicidal activity was tested in 1998 by the Cantacuzino Institute on Candida albicans and Aspergillus niger using drug solutions 1B and 2B. The same three test methods were used: the diffusimetric method, the substance incorporation method

RO 114943 Β1 to be tested in the culture medium and the time-limited contact method between the test solution and the test microorganism. The conclusion of the tests was the following: by means of the diffusimetric method, the inhibition of the development of the test microorganisms was obtained in the diffusion area of the solutions; by the method of incorporating the test substance into the culture medium, inhibition of the growth of all species of tested microorganisms was also obtained, for amounts greater than or equal to 1 ml of solution incorporated in 15 ml of culture medium.

Solutions 1B and 2B have fungicidal action, producing after 30 min of contact a significant decrease in the number of fungal colonies.

Antiviral activity was tested in 1998 at the Cantacuzino Institute on influenza virus APR/8/34 using solutions 1 A, 2A, 3A, 1B, 2B, 3B, 2Bb, 2Br. The method used was the inoculation in the intraallantoic fluid of embryonated chicken eggs with an 11-day embryo. It was consistently found that the 2Bb solution inhibits the multiplication of the influenza virus after 30 min by 100%, and the 2Br solution inhibits the viral multiplication by 75% after 60 min of preliminary contact with the virus.

The antineoplastic activity was tested in 1998 in vitro at the Cantacuzino Institute.

The action of solutions 1B, 2B and 1A on HEp-2 human laryngeal carcinoma cells, cultured in vitro, was tested using two test systems: on a macrotumor, followed for 72 h and on a multiplying neopiazic cell (early tumor) followed for 96 h and using 30%, 60% and 90% concentrations for drug solutions.

For solution 1B of 30% and 60% concentration, a slow reduction of cell density in the macrotumor during 72 h and a reduction of early tumor development during 96 h was observed.

For the same solution, with a concentration of 90%, a drastic reduction (7-8 times) of the cell density in the macrotumor was found, over a period of 72 h and a total inhibition of the development of the incipient tumor, followed over a period of 96 hours .

For solution 1A of 30% and 60% concentrations, the brutal reduction of the cell density in the macrotumor was found over a period of 72 h and slightly higher cell mortality compared to the control sample in the case of the incipient tumor, over a period of 96 hours.

For the same 90% concentration solution, it was found that the cell density is placed at lower values for a duration of 72 h compared to a duration of 24 h, for the macrotumor and the neopiazic cell mortality is much superior compared to the control in the early tumor, on during 96 hours.

in the case of solution 2B of 30% and 60% concentration, a brutal and important reduction of the cell density was found in the case of the macrotumor, over a period of 72 h.

For the 90% concentration of solution 2B, a total destruction of the macrotumor was found over a period of 72 h.

Solution 2B does not allow cell growth in culture, at any of the three concentrations tested, in the early tumor.

Comparing the effect on stationary or growing HEp-2 cells for the three tested solutions, increasing cytotoxicity was observed in the order 1A, 1B, 2B.

Also in 1998, in vitro tests were performed with solution 2B on the Hela cell line - human cervical carcinoma, in concentrations of 30%, 60% and 90%. Testing was done on stationary cells (macrotumor) and multiplying cells (incipient tumor) for 72 h and 96 h, respectively.

The result was a brutal and significant reduction in cell density at 24 and 72 h of contact, for all concentrations tested, namely by about 1/3 in the case of concentrations of 30% and 60% and by about 1/4-4.5 of control value for concentration of 90%, in case of macrotumor.

Claims (15)

RO 114943 Β1 in the case of the incipient tumor, it resulted that the Hela cells do not multiply in vitro under the influence of solution 2B, presenting still after one hour of contact a mortality of 85-95% for all three tested solution concentrations. Type 1A solution is used in the first phase of treatment. Solutions 1B and 2B are used in the second phase of treatment. Solutions 2Bb and 2Br differ from solution 2B in the concentration of the component elements. 250 Examples of application of the treatment method are presented below. Example 1. The method of treatment according to the invention can be applied for conditions caused by viruses, bacteria and fungi. With the presented process and apparatus, an electronegative ionic solution is made, containing K ⁺¹ ions. Administer this solution orally, twice a day, about 15 minutes before meals, in a quantity of 10 ml, for about 255 10 days. About 3 days after the end of this treatment, another electronegative ionic solution, containing Ca ⁺² , K ⁺¹ , Fe ⁺² ions, is administered orally, twice a day, about 15 minutes before meals, in amount of 5-10 ml, for 5-10 days. The cessation and involution of the condition are monitored periodically through medical analyses. Example 2. The treatment method according to the invention can be applied to stop 260 the evolution of neoplastic tumors and to destroy them. An electronegative ionic solution is made, containing K ⁺¹ ions, which is administered orally, twice a day, about 15 minutes before meals, in a quantity of 10 ml, for about 10 days. Approximately 3 days after the end of this treatment, another electronegative ionic solution is administered orally, containing Ca ²⁺ , Mg ²⁺ , Fe ³⁺ ions, which 265 is administered orally, twice a day, before of food every 15 minutes, in the amount of 5-10 ml, for 5-10 days. Stopping the progression of the disease and destroying the tumor are monitored periodically through medical tests. Both solid (carcinoma) and liquid neoplastic cells are treated in this way. Example 3. The treatment method according to the invention can also be applied externally. An electronegative ionic solution is made, containing K ⁺¹ ions, with which the affected area is wiped, after which, with an electronegative ionic solution containing K ⁺¹ , Ca ⁺² , Mg ⁺² ions, the affected portion is painted. Healing takes place from the inside out, in a short time and without leaving traces. depending on the degree of infection of the affected site, treatment with 275 electropositive ionic solution can also be used, in the situation where the wound is very deep and heavily infected. Experiments were also made to use the treatment method according to the invention in diseases of the bone system, the muscular system, the digestive tube, the circulatory system, the urinary system, the nervous system, the glandular system, the blood. The experiments were done on human volunteers. following medical analyses, the 280 medicine ionic solutions contained those elements specific to the affected organ, treating the cause that determined the appearance of the condition and obtaining positive results for all these treated organs. Claims 285 1. Apparatus for obtaining medicinal solutions, characterized in that, in order to obtain ionic medicinal solutions, it consists of an electrolysis vessel (1), made of glass, provided at the top with a cover (7) made of material plastic, on the lower part of which two electrodes (2a, 2b) and a diaphragm (5) are fixed, on the electrode (2a), outside the vessel (1), a rectifier diode (3) is fixed, connected to a wire of a power cord (4), the other wire being soldered to the electrode (2b). RO 114943 Β1 2. Apparatus according to claim 1, characterized in that the electrodes (2a, 2b) are made of stainless steel with a content of Cr, Ni, Mo in the proportions 18/8/3, type W4A. 3. Apparatus according to claim 1, characterized in that, in another constructive variant, the electrode (2b) is made of Ti, and the electrode (2a) is made of W4A type steel. 4. Apparatus according to claim 1, characterized in that, in another constructive variant, the electrode (2b) is made of Ag, and the electrode (2a) is made of W4A type steel. 5. Apparatus according to claims 1, 2, 3 and 4, characterized in that the ratio between the length and width of the electrodes is about 1/3, and their thickness is about 2 mm. 6. Apparatus according to claims 1, 2, 3, 4 and 5, characterized in that the diaphragm (5) is made of cotton. 7. Apparatus according to claim 6, characterized in that the diaphragm (5) is made of natural silk, obtained from 20 den x2 cabled thread. 8. Process for obtaining medicinal solutions, characterized by the fact that, using the electrochemical process, carried out by electrolysis, it allows obtaining any element from the periodic system of elements, which can be extracted, purified and activated, the activation being done by increasing the energy level of the electrons from the last orbit, which ensures the maximum reactivity of the element used, these pure and activated elements being found in the ionic solutions prepared using the apparatus made according to claim 1, the process comprising a first phase in which the stoichiometric calculation of the components entering into the composition of the ionic solution to be prepared, a second phase in which the qualitative analysis of the water used for electrolysis is carried out, determining the amount and types of impurities in the water, a third phase in which the water is purified by simple electrolysis, for a duration of between two minutes and twelve minutes, depending on the water hardness, a fourth phase in which the water purified by electrolysis and electronegatively charged is decanted and used for the preparation of ionic-drug solutions, a fifth phase in which the amount of electronegatively charged water required, a sixth phase in which the electronegatively charged water and the stoichiometrically calculated components are mixed in the electrolyzer, a seventh phase in which the electrolysis vessel (1) is covered with the lid (7), on which the electrodes (2a, 2b) are fixed ) and the diaphragm (5) and the electrolysis is carried out, obtaining the solution with the pure elements of interest, the duration of the electrolysis being between two minutes and twelve minutes, depending on the concentration of the components, an eighth phase in which the part of the solution that is not of interest is evacuated and a ninth phase in which, on the solution containing pure elements, electrolysis is repeated in order to activate them. 9. Method of cellular treatments, characterized in that it uses drugs prepared in the form of ionic solutions, made with the apparatus according to claim 1, using the process presented in claim 8 and is based on the creation in the body of ionic channels of appropriate polarity, which allow fixation the components of the ionic solutions-medicine in the affected organ and in a first phase, in order to dislodge the diseased cells, simultaneously with the revitalization and energization of the affected area as well as the whole body, an ionic solution is administered in doses and at predetermined time intervals, and in -a second phase acts on the affected area, aiming for the total destruction of the agents that caused the condition, by using other ionic solutions, of a composition and concentration corresponding to the disease being treated, the respective ionic solutions being calculated in ml/kbody and applied according to body weight and age, the second phase of treatment being repeated, if medical analyzes indicate the need for this. RO 114943 Β1 10. Method according to claim 9, characterized by the fact that it can be applied for diseases caused by viruses, bacteria and fungi and consists in the administration, orally, twice a day, about 15 minutes before meals, of an electronegative ionic solution that contains, in a first phase, K ⁺¹ ions and is taken in a quantity of 10 ml for about 10 days, and in a second phase Ca ⁺² , K ⁺¹ , Fe ions ⁺² and it is taken in an amount of 5-10 ml for 5-10 days, after the passage of three days from the end of the first phase, the cessation and involution of the condition being monitored periodically through medical tests, which indicate the eventual necessity of the resumption of the second phase. 11. Treatment method according to claim 9, characterized in that it is applied to stop the evolution of neoplastic tumors and destroy them using an electronegative ionic solution, which is administered orally, twice a day, about 15 minutes before meals and which contains, in a first phase, ions of K ⁺¹ and is taken in a quantity of 350 approx. 10 ml, for approx. 10 days, and in a second phase it contains ions of Ca ²⁺ , Mg ²⁺ , Fe ^{+ 3} and is taken in an amount of 5-10 ml, for 5-10 days, after three days from the end of the first phase, stopping the evolution of the disease and destroying the tumor, followed periodically by medical analyses, which indicate the need for the possible resumption of the phase of two. 12. Treatment method according to claim 11, characterized in that it is applied to solid neoplastic cells. 13. Treatment method according to claim 11, characterized in that it is applied to liquid neoplastic cells. 14. Treatment method according to claim 9, characterized in that it is applied externally and uses in a first phase an electronegative ionic solution, containing 360 K ⁺¹ ions, with which the affected area is erased, and in a second phase, an electronegative ionic solution, containing K ⁺¹ , Ca ⁺² , Mg ⁺² ions, with which the affected portion is painted. 15. Method according to claims 10,11,12,13 and 14, characterized in that electropositive ionic solutions are used in the case of digestive tubes with hypoacidity and in the case when the condition is in an advanced stage due to strong pathogens, 365 civil and who have no medical remedy. The president of the examination committee: Eng. Popescu Livia Examiner: Eng. Dumitru Daniela