PL99288B1 - METHOD OF MANUFACTURING AN AGENT FOR THE PROTECTION OF NEEDLES - Google Patents
METHOD OF MANUFACTURING AN AGENT FOR THE PROTECTION OF NEEDLES Download PDFInfo
- Publication number
- PL99288B1 PL99288B1 PL18917776A PL18917776A PL99288B1 PL 99288 B1 PL99288 B1 PL 99288B1 PL 18917776 A PL18917776 A PL 18917776A PL 18917776 A PL18917776 A PL 18917776A PL 99288 B1 PL99288 B1 PL 99288B1
- Authority
- PL
- Poland
- Prior art keywords
- sawdust
- dry
- beech
- pine bark
- agent
- Prior art date
Links
- 238000004519 manufacturing process Methods 0.000 title description 6
- 241001070947 Fagus Species 0.000 claims description 11
- 235000010099 Fagus sylvatica Nutrition 0.000 claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 235000010204 pine bark Nutrition 0.000 claims description 9
- 239000000203 mixture Substances 0.000 claims description 7
- 241000878003 Dendrolycopodium obscurum Species 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 5
- 241001634106 Phlebiopsis gigantea Species 0.000 claims description 4
- 208000015181 infectious disease Diseases 0.000 claims description 4
- 238000001816 cooling Methods 0.000 claims description 3
- 229920000742 Cotton Polymers 0.000 claims description 2
- 239000001888 Peptone Substances 0.000 claims description 2
- 108010080698 Peptones Proteins 0.000 claims description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 2
- 229930006000 Sucrose Natural products 0.000 claims description 2
- 238000011049 filling Methods 0.000 claims description 2
- 239000011521 glass Substances 0.000 claims description 2
- 235000019319 peptone Nutrition 0.000 claims description 2
- 238000002360 preparation method Methods 0.000 claims description 2
- 239000008223 sterile water Substances 0.000 claims description 2
- 239000005720 sucrose Substances 0.000 claims description 2
- 239000003795 chemical substances by application Substances 0.000 description 10
- 239000000758 substrate Substances 0.000 description 7
- 239000000725 suspension Substances 0.000 description 5
- 239000003755 preservative agent Substances 0.000 description 3
- 230000002335 preservative effect Effects 0.000 description 3
- 208000030852 Parasitic disease Diseases 0.000 description 2
- 238000000354 decomposition reaction Methods 0.000 description 2
- 238000013467 fragmentation Methods 0.000 description 2
- 238000006062 fragmentation reaction Methods 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 1
- 235000011613 Pinus brutia Nutrition 0.000 description 1
- 241000018646 Pinus brutia Species 0.000 description 1
- 239000007900 aqueous suspension Substances 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000011928 denatured alcohol Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000001061 forehead Anatomy 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000009533 lab test Methods 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 239000011814 protection agent Substances 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000002255 vaccination Methods 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Landscapes
- Medicines Containing Plant Substances (AREA)
Description
Przedmiotem wynalazku jest sposób wytwarzania srodka do zabezpieczania pniaków potrzebiezowych, zwlaszcza przed infekcja pierwotna ze strony huby korzeni. Umozliwia on stosowanie metody biologicznej ochrony lasu przed pasozytniczymi chorobami korzeni drzew lesnych oraz wskutek szybszego rozkladu pniaków, ograniczania rozwoju zyjacych w nich owadów.The subject of the invention is a method for the production of a preparation for securing stub stumps, especially against primary infection by root hubs. It allows the use of a biological method protection of the forest against parasitic diseases of forest tree roots and as a result of faster decomposition of stumps, limiting the development of insects living in them.
Znany srodek do zabezpieczania pniaków sosnowych pod nazwa „P.g.suspension Ecological Laboratories England" jest suspensja zarodnikowa grzyba Phlebia gigantea (Fr.)Donk w srodku konserwujacym. Wytwarzana ona jest poprzez hodowle grzyba na powierzchni klocków drewnianych z których jego zarodniki — oidia — sa wyplukiwane, wirowane i liofilizowane a nastepnie zageszczane i stabilizowane. Wymaga to stosowania specjalnej technologii i aparatury oraz odpowiednich warunków jalowego pakowania i przechowywania w niskich temperaturach. Srodek ten wykazuje w normalnych warunkach trwalosc 6 do 12 miesiecy. > Celem wynalazku jest usuniecie tych niedogodnosci i opracowanie prostego sposobu wytwarzania srodka o zwiekszonej skutecznosci i trwalosci, latwego do przechowywania. Cel ten osiagnieto przez zastosowanie odpowiedniego substratu dla rozwoju zarodnikujacej kultury Phlebia gigantea, rozwijajacej sie w sposób ciagly.Known agent for protecting pine stumps under the name "P.g.suspension Ecological Laboratories England "is a spore suspension of the fungus Phlebia gigantea (Fr.) Donk in a preservative. she is by growing the fungus on the surface of wooden blocks from which its spores - oidia - sa rinsed, centrifuged and freeze-dried, and then concentrated and stabilized. This requires a special application technology and apparatus as well as appropriate conditions of sterile packaging and storage in low conditions temperatures. This product has a shelf life of 6 to 12 months under normal conditions. > The aim of the invention is to overcome these drawbacks and to provide a simple method of producing the agent with increased efficiency and durability, easy to store. This goal was achieved by application a suitable substrate for the development of a continuously developing sporulating culture of Phlebia gigantea.
Rozwiazanie zadania polega na zastosowaniu takiego substratu do produkcji srodka do zabezpieczania pniaków, który zapewnia trwaly rozwój zarodnikujacej grzybni, odpowiednie zageszczenie oidiów i mozliwosc wytwarzania srodka we wlasnym zakresie przez jednostki gospodarczo-lesne, oraz jego skutecznosc dzialania do 3 lat. • W sposobie wedlug wynalazku jako podloze zastosowano swieze trociny bukowe ze zdrowego drewna lub mieszanine tych trocin ze zmielona kora sosny w stosunku 40 czesci wagowych kory i 60 czesci wagowych trocin.The solution to the task is to use such a substrate for the production of a stump protection agent, which ensures the sustainable development of the sporulating mycelium, the proper concentration of oidia and the possibility of production of the measure on their own by economic and forest entities, and its effectiveness to 3 years. • In the method according to the invention, fresh beech sawdust of healthy wood or is used as the substrate a mixture of these sawdust with ground pine bark in a ratio of 40 parts by weight of bark and 60 parts by weight sawdust.
Trociny ze zmielona kora sosny, jako podloze do trwalej produkcji srodka, sa bardziej efektywne niz same trociny bukowe z uwagi na zwielokrotniona powierzchnie substratu na której odbywa sie fragmentacja grzybni na oidia. Trociny bukowe zapewniaja szybka kolonizacje grzybni, sa zródlem niezbednych skladników2 99 288 pokarmowych i umozliwiaja utrzymanie srodka w stanic ciaglej aktywnosci fizjologicznej. Kora sosny, ulegajac w mniejszym stopniu rozkladowi, zwieksza trwalosc srodka, a powodujac powstawanie oidiów równiez wewnatrz komórek kory, przyczynia sie do zwiekszenia stopnia fragmentacji. Uzycie kory jest wskazane zwlaszcza w przypadku kiedy przewiduje sie dluzy niz dwa lata okres czasu od zalozenia kultury do zastosowania w terenie srodka do zabezpieczania pniaków.Sawdust with ground pine bark, as a substrate for the sustainable production of the agent, is more effective than alone beech sawdust due to the multiplied surface of the substrate on which the fragmentation of the mycelium takes place on oidia. Beech sawdust ensures quick colonization of mycelium and is a source of the necessary ingredients2 99 288 nutrients and keep the agent in constant physiological activity. Pine bark, yielding decomposition to a lesser extent, increases the durability of the agent and causes the formation of oidia also inside the cells of the cortex, it contributes to an increase in the degree of fragmentation. The use of bark is advisable especially when the period from the foundation of the culture to field application of a stump preservative.
Istota sposobu wedlug wynalazku polega na nawilzeniu do wilgotnosci 60—70% suchych trocin bukowych lub suchej mieszaniny trocin z kora sosny w stosunku 40 czesci wagowych zmielonej kory sosny i 60 czesci wagowych trocin bukowych. Do nawilzania stosuje sie roztwór zawierajacy wode z cukrem w.stosunku 1 kg cukru na 101 wody. w ilosci 101 roztworu na 8 kg suchych trocin lub suchej mieszaniny kory sosny i trocin bukowych* Nastepnie sterylizuje sie w parowniku elektrycznym trzykrotnie po jednej godzinie od momentu ukazania sie pary wodnej, z przerwami umozliwiajacymi pelne wystudzenie, przenosi sie goracy substrat do przemytych spirytusem denaturowanym pojemników, najlepiej szklanych, wypelniajac je do 2/3 ich objetosci i po wystudzeniu do temperatury 20—25°C zaprawia sie roztworem przyspieszajacym rozwój grzybni, zawierajacym 2 g peptonu, 20 g sacharozy w 100 ml jalowej wody na 5 kg suchych trocin lub suchej mieszaniny trocin z kora oraz zalewa sie woda zarodnikowa sporzadzona z kultur grzyba Phlebia gigantea (Fr.)Donk i pojemniki zakrywa korkiem z waty. < W okresie od 3 do 5 miesiecy od chwili zaszczepienia grzybem, pojemniki ze srodkiem do zabezpieczania pniaków przechowuje sie w temperaturze pokojowej (18—22°C), mieszajac zawartosc kilkakrotnie (3-5 razy), np. przez przetaczanie, nie otwierajac ich; po tym okresie - w temperaturze obnizonej do okolo 10—15°C, w której zachowuje on skutecznosc dzialania do 3 lat.The essence of the method according to the invention consists in moistening the dry beech sawdust to a humidity of 60-70%. or a dry mixture of sawdust with pine bark in the ratio of 40 parts by weight of ground pine bark and 60 parts by weight of beech sawdust. For moisturizing, a solution containing water with sugar in the ratio of 1 kg is used sugar in 101 water. in the amount of 101 solution per 8 kg of dry sawdust or a dry mixture of pine bark and sawdust beech * Then sterilized in an electric steamer three times one hour from the moment the appearance of water vapor, with breaks allowing for full cooling, the hot substrate is transferred to containers washed with denatured alcohol, preferably glass containers, filling them up to 2/3 of their volume and after cooling to 20-25 ° C, it is seasoned with a solution that accelerates the development of mycelium, containing 2 g of peptone, 20 g of sucrose in 100 ml of sterile water for 5 kg of dry sawdust or a dry mixture sawdust from the bark and spore water made of Phlebia gigantea (Fr.) Donk cultures and the containers are covered with a cotton wool stopper. < In the period of 3 to 5 months from the time of mushroom inoculation, containers with a preservative stumps are stored at room temperature (18-22 ° C), by mixing the contents several times (3-5 times), eg by rolling, without opening them; after this period - at a temperature reduced to about 10-15 ° C, in which it remains effective for up to 3 years.
Srodek jest gotowy do uzycia z chwila ukazania sie na powierzchni i w calej masie substratu bialej, widocznej golym okiem grzybni. W celu zachowania ciaglosci produkcji zaleca sie zachowanie czesci uzyskanego srodka jako szczepionki z której przygotowuje sie wode zarodnikowa do nastepnych szczepien.The agent is ready for use as soon as it appears on the surface and in the entire mass of the white substrate, mycelium visible to the naked eye. In order to maintain continuity of production it is recommended to keep the obtained part the agent as a vaccine from which the spore water is prepared for the next vaccination.
Forma uzytkowa srodka jest zawiesina wodna przygotowana przez zmieszanie 1—2 czesci wagowych srodka z 10 czesciami wagowymi wody. Dziesiec litrów zawiesiny wystarcza na zabezpieczenie 800 pniaków srednicy —25 cm* Zainfekowanie pniaków nastepuje przez nalozenie zawiesiny na powierzchnie swiezo scietego pniaka.The useable form of the agent is an aqueous suspension prepared by mixing 1-2 parts by weight of the agent with 10 parts by weight of water. Ten liters of slurry is enough to protect 800 stumps in diameter —25 cm * Stumps are infected by applying the suspension to the surface of a freshly cut stump.
Przed nalozeniem zawiesiny nalezy wykonac siekiera lub pila mechaniczna kilka naciec ulatwiajacych wnikanie i zatrzymanie srodka wewnatrz pniaka. Po nalozeniu zawiesiny czolo pniaka nalezy przykryc sciólka w celu zabezpieczenia przed wysychaniem. ¦ Skutecznosc otrzymanego sposobem wedlug wynalazku srodka stwierdzono w badaniach laboratoryjnych i terenowych na podstawie oceny liczby pniaków zabezpieczonych przed infekcja ze strony huby korzeni. ¦ Uzyskano 100% udatnosc zabiegu a ponad 80% pniaków potraktowanych srodkiem uleglo znacznemu rozkladowi juz po uplywie roku. Uzyskano przez to nie tylko skuteczne ograniczenie infekcji pierwotnej ze strony huby korzeni, lecz równiez wprowadzono w srodowisko lesne aktywnie dzialajacy czynnik walki biologicznej z innymi pasozytniczymi chorobami korzeni drzew lesnych. -Before applying the suspension, make an ax or a chainsaw a few cuts to facilitate penetration and stopping the center inside the stump. After applying the suspension, cover the stump's forehead with litter protection against drying out. ¦ The effectiveness of the agent obtained according to the invention was found in laboratory tests and field based on the assessment of the number of stumps protected against infection by root hubs. ¦ The treatment success rate was 100%, and over 80% of treated stumps underwent significant decomposed after one year. The result was not only an effective reduction of the primary infection with root hubs, but also introduced into the forest environment an active fighting factor biological with other parasitic diseases of forest tree roots. -
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL18917776A PL99288B1 (en) | 1976-04-29 | 1976-04-29 | METHOD OF MANUFACTURING AN AGENT FOR THE PROTECTION OF NEEDLES |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL18917776A PL99288B1 (en) | 1976-04-29 | 1976-04-29 | METHOD OF MANUFACTURING AN AGENT FOR THE PROTECTION OF NEEDLES |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| PL99288B1 true PL99288B1 (en) | 1978-06-30 |
Family
ID=19976641
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PL18917776A PL99288B1 (en) | 1976-04-29 | 1976-04-29 | METHOD OF MANUFACTURING AN AGENT FOR THE PROTECTION OF NEEDLES |
Country Status (1)
| Country | Link |
|---|---|
| PL (1) | PL99288B1 (en) |
-
1976
- 1976-04-29 PL PL18917776A patent/PL99288B1/en unknown
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