PL447279A1 - Zwiększenie specyficzności identyfikacji jednonukleotydowych polimorfizmów genetycznych w układach izotermalnych poprzez zastosowanie oligonukleotydów z powtarzajacymi się modyfikacjami typu LNA - Google Patents

Zwiększenie specyficzności identyfikacji jednonukleotydowych polimorfizmów genetycznych w układach izotermalnych poprzez zastosowanie oligonukleotydów z powtarzajacymi się modyfikacjami typu LNA

Info

Publication number
PL447279A1
PL447279A1 PL447279A PL44727923A PL447279A1 PL 447279 A1 PL447279 A1 PL 447279A1 PL 447279 A PL447279 A PL 447279A PL 44727923 A PL44727923 A PL 44727923A PL 447279 A1 PL447279 A1 PL 447279A1
Authority
PL
Poland
Prior art keywords
lna
oligonucleotides
repeating
genetic polymorphism
increased specificity
Prior art date
Application number
PL447279A
Other languages
English (en)
Inventor
Dawid NIDZWORSKI
Sabina ŻOŁĘDOWSKA
Kasjan SZEMIAKO
Tomasz Domoradzki
Marta SKWARECKA
Original Assignee
Instytut Biotechnologii I Medycyny Molekularnej
Geneme Molecular Spółka Z Ograniczoną Odpowiedzialnością
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Instytut Biotechnologii I Medycyny Molekularnej, Geneme Molecular Spółka Z Ograniczoną Odpowiedzialnością filed Critical Instytut Biotechnologii I Medycyny Molekularnej
Priority to PL447279A priority Critical patent/PL447279A1/pl
Priority to EP24223070.4A priority patent/EP4585700A3/en
Publication of PL447279A1 publication Critical patent/PL447279A1/pl

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/186—Modifications characterised by incorporating a non-extendable or blocking moiety

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Przedmiotem niniejszego zgłoszenia jest nowa konformacja modyfikacji typu LNA w oligonukleotydach, umożliwiająca specyficzną identyfikację jednonukleotydowych mutacji (SNP) w genomowy DNA oraz metoda jej wykorzystania.
PL447279A 2023-12-23 2023-12-23 Zwiększenie specyficzności identyfikacji jednonukleotydowych polimorfizmów genetycznych w układach izotermalnych poprzez zastosowanie oligonukleotydów z powtarzajacymi się modyfikacjami typu LNA PL447279A1 (pl)

Priority Applications (2)

Application Number Priority Date Filing Date Title
PL447279A PL447279A1 (pl) 2023-12-23 2023-12-23 Zwiększenie specyficzności identyfikacji jednonukleotydowych polimorfizmów genetycznych w układach izotermalnych poprzez zastosowanie oligonukleotydów z powtarzajacymi się modyfikacjami typu LNA
EP24223070.4A EP4585700A3 (en) 2023-12-23 2024-12-23 Isothermal nucleic acid amplification using lna modified primers

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL447279A PL447279A1 (pl) 2023-12-23 2023-12-23 Zwiększenie specyficzności identyfikacji jednonukleotydowych polimorfizmów genetycznych w układach izotermalnych poprzez zastosowanie oligonukleotydów z powtarzajacymi się modyfikacjami typu LNA

Publications (1)

Publication Number Publication Date
PL447279A1 true PL447279A1 (pl) 2025-06-30

Family

ID=94733053

Family Applications (1)

Application Number Title Priority Date Filing Date
PL447279A PL447279A1 (pl) 2023-12-23 2023-12-23 Zwiększenie specyficzności identyfikacji jednonukleotydowych polimorfizmów genetycznych w układach izotermalnych poprzez zastosowanie oligonukleotydów z powtarzajacymi się modyfikacjami typu LNA

Country Status (2)

Country Link
EP (1) EP4585700A3 (pl)
PL (1) PL447279A1 (pl)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107338289A (zh) * 2017-06-20 2017-11-10 复旦大学附属华山医院 一种锁核酸修饰的lamp引物组合物及其应用
WO2022101259A1 (en) * 2020-11-10 2022-05-19 MultiplexDX, s.r.o. Loop mediated isothermal nucleic acid amplification (lamp) using lna-modified primers and a metal-based colorimetric method for detecting an amplification product
WO2023069026A2 (en) * 2021-10-22 2023-04-27 Nanyang Technological University A method for detection of point mutations in target nucleic acid using loop-mediated isothermal amplification

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107338289A (zh) * 2017-06-20 2017-11-10 复旦大学附属华山医院 一种锁核酸修饰的lamp引物组合物及其应用
WO2022101259A1 (en) * 2020-11-10 2022-05-19 MultiplexDX, s.r.o. Loop mediated isothermal nucleic acid amplification (lamp) using lna-modified primers and a metal-based colorimetric method for detecting an amplification product
WO2023069026A2 (en) * 2021-10-22 2023-04-27 Nanyang Technological University A method for detection of point mutations in target nucleic acid using loop-mediated isothermal amplification

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
MAERTENS O ET AL.: "Anal Biochem. 2006 Dec 1;359(1):144-6", REAL-TIME QUANTITATIVE ALLELE DISCRIMINATION ASSAY USING 3' LOCKED NUCLEIC ACID PRIMERS FOR DETECTION OF LOW-PERCENTAGE MOSAIC MUTATIONS *
MOURITZEN P ET AL.: "Expert Rev Mol Diagn. 2003 Jan;3(1):27-38", SINGLE NUCLEOTIDE POLYMORPHISM GENOTYPING USING LOCKED NUCLEIC ACID (LNA) *

Also Published As

Publication number Publication date
EP4585700A3 (en) 2025-09-24
EP4585700A2 (en) 2025-07-16

Similar Documents

Publication Publication Date Title
US11821030B2 (en) Multiplex targeted amplification using flap nuclease
Erdogan et al. Detection of mitochondrial single nucleotide polymorphisms using a primer elongation reaction on oligonucleotide microarrays
Krjutškov et al. Development of a single tube 640-plex genotyping method for detection of nucleic acid variations on microarrays
Barker et al. Two methods of whole-genome amplification enable accurate genotyping across a 2320-SNP linkage panel
Fouse et al. Genome-scale DNA methylation analysis
Ellonen et al. Development of SNP microarray for supplementary paternity testing
DE602005027067D1 (de) Nachweis chromosomaler störungen
Miller et al. High-density single-nucleotide polymorphism maps of the human genome
JP2017519774A5 (pl)
ATE516371T1 (de) Oligonukleotide und deren verwendungen
Kling et al. DNA microarray as a tool in establishing genetic relatedness—Current status and future prospects
JP2013544498A5 (pl)
BR0308134A (pt) análise rápida das variações em um genoma
ATE496998T1 (de) Verfahren zur fehlerverringerung in nukleinsäurepopulationen
Myllykangas et al. Efficient targeted resequencing of human germline and cancer genomes by oligonucleotide-selective sequencing
Gao et al. Forensic genetic informativeness of an SNP panel consisting of 19 multi-allelic SNPs
Ling et al. Evaluation of genome coverage and fidelity of multiple displacement amplification from single cells by SNP array
Li et al. Selection of 29 highly informative InDel markers for human identification and paternity analysis in Chinese Han population by the SNPlex genotyping system
Bruse et al. Improvements to bead-based oligonucleotide ligation SNP genotyping assays
CN105431555A (zh) 用于亚硫酸氢盐转化的序列捕获的组合物和方法
Somridhivej et al. Characterization, polymorphism assessment, and database construction for microsatellites from BAC end sequences of channel catfish (Ictalurus punctatus): A resource for integration of linkage and physical maps
Lv et al. HD-Marker: a highly multiplexed and flexible approach for targeted genotyping of more than 10,000 genes in a single-tube assay
Ehses et al. Optimization and design of oligonucleotide setup for strand displacement amplification
Kalthoff et al. High resolution melting analysis: rapid and precise characterisation of recombinant influenza A genomes
DE602006018861D1 (de) Schnelle komparative genomhybridisierung