PL441984A1 - Method of obtaining a collagen matrix from bones and the collagen matrix obtained by this method and use of the collagen matrix - Google Patents
Method of obtaining a collagen matrix from bones and the collagen matrix obtained by this method and use of the collagen matrixInfo
- Publication number
- PL441984A1 PL441984A1 PL441984A PL44198422A PL441984A1 PL 441984 A1 PL441984 A1 PL 441984A1 PL 441984 A PL441984 A PL 441984A PL 44198422 A PL44198422 A PL 44198422A PL 441984 A1 PL441984 A1 PL 441984A1
- Authority
- PL
- Poland
- Prior art keywords
- collagen matrix
- sub
- sup
- dried
- stage
- Prior art date
Links
- 239000011159 matrix material Substances 0.000 title abstract 10
- 102000008186 Collagen Human genes 0.000 title abstract 9
- 108010035532 Collagen Proteins 0.000 title abstract 9
- 229920001436 collagen Polymers 0.000 title abstract 9
- 210000000988 bone and bone Anatomy 0.000 title abstract 5
- 238000000034 method Methods 0.000 title abstract 5
- 239000000463 material Substances 0.000 abstract 4
- 235000015278 beef Nutrition 0.000 abstract 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 abstract 2
- 239000002994 raw material Substances 0.000 abstract 2
- 210000004872 soft tissue Anatomy 0.000 abstract 2
- 230000007547 defect Effects 0.000 abstract 1
- 239000008367 deionised water Substances 0.000 abstract 1
- 229910021641 deionized water Inorganic materials 0.000 abstract 1
- 238000005115 demineralization Methods 0.000 abstract 1
- 230000002328 demineralizing effect Effects 0.000 abstract 1
- 238000000605 extraction Methods 0.000 abstract 1
- 229910052500 inorganic mineral Inorganic materials 0.000 abstract 1
- 239000011707 mineral Substances 0.000 abstract 1
- 230000003239 periodontal effect Effects 0.000 abstract 1
- 239000011148 porous material Substances 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
Landscapes
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Materials For Medical Uses (AREA)
- Cosmetics (AREA)
Abstract
Przedmiotem zgłoszenia jest sposób otrzymywania matrycy kolagenowej z kości wołowych, w którym kości wołowe oczyszcza się, demineralizuje, sterylizuje i liofilizuje, który charakteryzuje się tym, że obejmuje następujące etapy: a) kości wołowe oczyszcza się z tkanki miękkiej i tnie na kawałki o objętości 0,25 - 16 cm<sup>3</sup>, b) surowiec przygotowany w etapie a) myje się wodą odsącza i osusza, c) osuszony surowiec poddaje się procesowi usunięcia tłuszczy w procesie ekstrakcji CO<sub>2</sub> fazie nadkrytycznej, d) materiał uzyskany w etapie c) poddaje się demineralizacji w roztworze kwasu solnego o stężeniu 0,05 - 1 mol/dm<sup>3</sup> przez okres 24 godzin, e) materiał pozbawiony składników mineralnych uzyskany w etapie d) w postaci surowej matrycy kolagenowej myje się wodą dejonizowaną, f) surową matrycę kolagenową poddaje się dezynfekcji w roztworze H<sub>2</sub>O<sub>2</sub> przez okres od 1do 4 godzin, przy stosunku objętościowym ilości materiału do wody 1:50, g) zdezynfekowaną matrycę zamraża się do temperatury nie niższej niż – 40°C, h) zamrożony materiał poddaje się liofilizacji. Otrzymaną matrycę kolagenową można umieścić się w roztworze substancji wzbogacającej o stężeniu od 0,5 do 5% v/w. Według sposobu otrzymuje się matrycę kolagenową w formie porowatych bloczków o średnicy porów 50 - 500 mikrometra. Matryce kolagenową stosuje się do regeneracji ubytków kostnych oraz tkanek miękkich przyzębia.The subject of the application is a method for obtaining a collagen matrix from beef bones, in which the beef bones are cleaned, demineralized, sterilized and freeze-dried, which is characterized by the following steps: a) beef bones are cleaned of soft tissue and cut into pieces with a volume of ,25 - 16 cm<sup>3</sup>, b) the raw material prepared in stage a) is washed with water, drained and dried, c) the dried raw material is subjected to the process of fat removal in the CO<sub>2</sub> extraction process supercritical phase, d) the material obtained in stage c) is subjected to demineralization in a hydrochloric acid solution with a concentration of 0.05 - 1 mol/dm<sup>3</sup> for a period of 24 hours, e) material devoid of mineral components obtained in stage d) in the form of a raw collagen matrix, it is washed with deionized water, f) the raw collagen matrix is disinfected in a H<sub>2</sub>O<sub>2</sub> solution for a period of 1 to 4 hours, at a volume ratio amount of material to water 1:50, g) the disinfected matrix is frozen to a temperature not lower than - 40°C, h) the frozen material is freeze-dried. The obtained collagen matrix can be placed in a solution of an enriching substance with a concentration of 0.5 to 5% v/w. According to the method, a collagen matrix is obtained in the form of porous blocks with a pore diameter of 50 - 500 micrometers. The collagen matrix is used to regenerate bone defects and periodontal soft tissues.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL441984A PL441984A1 (en) | 2022-08-08 | 2022-08-08 | Method of obtaining a collagen matrix from bones and the collagen matrix obtained by this method and use of the collagen matrix |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL441984A PL441984A1 (en) | 2022-08-08 | 2022-08-08 | Method of obtaining a collagen matrix from bones and the collagen matrix obtained by this method and use of the collagen matrix |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| PL441984A1 true PL441984A1 (en) | 2024-02-12 |
Family
ID=89855719
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PL441984A PL441984A1 (en) | 2022-08-08 | 2022-08-08 | Method of obtaining a collagen matrix from bones and the collagen matrix obtained by this method and use of the collagen matrix |
Country Status (1)
| Country | Link |
|---|---|
| PL (1) | PL441984A1 (en) |
-
2022
- 2022-08-08 PL PL441984A patent/PL441984A1/en unknown
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