CN102363798B - Preparation process for collagen sponge - Google Patents
Preparation process for collagen sponge Download PDFInfo
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- CN102363798B CN102363798B CN 201110361186 CN201110361186A CN102363798B CN 102363798 B CN102363798 B CN 102363798B CN 201110361186 CN201110361186 CN 201110361186 CN 201110361186 A CN201110361186 A CN 201110361186A CN 102363798 B CN102363798 B CN 102363798B
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Abstract
The invention relates to a preparation method for collagen sponge. The preparation method comprises the following steps of: pretreating fresh pig skin serving as a raw material to remove fat; treating by an acid enzyme method; performing ultrasonic treatment so as to further improve extraction efficiency; purifying to prepare a high-purity collagen product; and preparing the collagen sponge through enzyme method crosslinking and freeze drying. By the preparation method for the collagen sponge, the extraction efficiency is high and the product purity reaches over 98 percent. The product comprises the collagen sponge which is prepared by freeze-drying active collagen and maintains a triple helix structure, has low immunogenicity, high biocompatibility and biodegradability, and is applicable to medical treatment.
Description
Technical field
The present invention relates to a kind of preparation method of collagen protein sponge, belong to the protein engineering field, this product is made through crosslinked by the active collagen that keeps triple-helix structure, has reduced immunogenicity, high-biocompatibility, and is biodegradable, is applicable to medical use.
Background technology
Collagen is that the vertebrates in-vivo content is the abundantest, the most widely one group of scleroprotein distributes, molecular weight is about 300000, all can form the supramolecule aggregation in the extracellular, be present in a large number in bone, cartilage, tendon and the skin, account for the 25%-33% of human body or other animal body total protein contents.The collagen that often contains several types in the same tissue is often take certain as main.Type i collagen is dispersed throughout the each several part of human body, is mainly in skin, tendon and the ligament, has very strong anti-Zhang Nengli.The II Collagen Type VI mainly is present in hyaline cartilage, the vitreum, has stronger anti-pressure ability.The III Collagen Type VI is distributed widely in the large tissue of extensibility, such as loose connective tissue etc., has extensibility and complaisance.Wherein, type i collagen accounts for 90% of the total collagen quantity of organism, and therefore, the use of type i collagen in biomaterial is also the most extensive.
Collagen is identical in the molecular level structure, is comprised of 3 a chain polypeptide, and each bar collagen chain all is the left hand helix configuration.Article 3, the left hand helix chain is wound in again the right-handed helix structure mutually, and namely superhelix is the triple helices structure of collagen protein uniqueness, makes its molecular structure highly stable.Article 3, chain is rich in glycine, proline(Pro) and L-Ala, lacks halfcystine and tryptophane, and tyrosine content is also very low, but contains unique hydroxylation amino acid (oxyproline and hydroxylysine).Because fibriilar directivity and the difference that becomes beam diameter and density, there is textural difference in the collagen of different tissues, and has function and structure feature separately.In reticular tissue, collagen is except mechanical support, or cell adhesion and mobile important substance basis, and therefore, collagen is considered to form occurrence factor important in fetal development and the tissue regeneration, is used widely in organizational project.
The collagen protein sponge main component is collagen protein, its similar reparation and regeneration that is well suited for human organ to the human collagen protein structure.Collagen protein can promote the growth of wound healing and granulation tissue, has good hemostasis and filling effect, can be used for wound healing, hemostasis, the filling of operation residual cavity etc.In the process of preparation porous support, should consider that the three-dimensional structure of timbering material and support is on the impact of cytoactive.Can promote to have good biocompatibility, and can keep certain vivo degradation speed the bioactive support of having of cell adhesion and growth concerning one.Support should have suitable mean pore size, keeps appropriate cell adhesion area when cell is moved between the hole, and collagen protein sponge is acknowledged as a kind of medical material of high-quality.
Disclose a kind of preparation method of collagen protein sponge among the CN101005865A, carried out crosslinkedly with glutaraldehyde, its collagen extraction efficient and purity are all lower, and linking agent uses the glutaraldehyde security lower.The disclosed method of prior art is often destroyed the triple-helix structure of collagen in the extraction preparation process of collagen, perhaps extraction efficiency is lower, can't realize can either retentive activity collagen triple-helix structure, improve simultaneously again extraction efficiency and purity, and with the collagen protein sponge of this collagen through the crosslinked preparation safety of enzyme process.
Summary of the invention
For above-mentioned situation, the purpose of this invention is to provide a kind of preparation method of collagen protein sponge.The present invention uses sour enzyme to add the preparation method of gradual ultrasonication in conjunction with processing, not only can keep the triple-helix structure of collagen, has improved significantly the extraction efficiency of collagen simultaneously, and crosslinked through enzyme process, the preparation collagen protein sponge.The present invention uses H
2O
2Solution-treated acid adding dissolved salt is analysed purifying and is added the purity that the dialysis purifying effectively raises the collagen product, has guaranteed the biocompatibility that collagen protein sponge is good.
A kind of preparation method of collagen protein sponge comprises the steps: that raw materials pretreatment, grease removal, sour enzyme are in conjunction with processing, ultrasonication, H
2O
2Solution-treated, sour dissolved salt are analysed purifying, dialysis purifying, enzyme process is crosslinked, dry.
Described raw material is to be selected from a kind of in fresh porcine skin, ox-hide, the ox heel string.
Described sour enzyme is in conjunction with being treated to: immerse in 0.6～0.7mol/L Glacial acetic acid and the pepsic mixed solution of 600～700mg/L through pretreated raw material, continue to stir about 25～30 hours.
Described ultrasonication preferably adopts gradual ultrasonication, uses first 100～200W supersound process 30 minutes, re-uses 200～300W supersound process 30 minutes, uses at last 300～400W supersound process 1 hour.
The preparation method of described collagen protein sponge, concrete steps are as follows:
(1) get fresh porcine skin, oil layer and trichocutis are removed in cutting, remove impurity, clean, pigskin is broken into fine granularity, according to 1: 30～1: 40 ratio of solid-to-liquid ratio, adds the aqueous sodium hydroxide solution of 0.01～0.03mol/L, in 6～8 ℃ of immersions 1～2 hour, filter, for subsequent use;
(2) adding quality in above-mentioned pretreated pigskin is anhydrous diethyl ether or the acetone of 6～8 times of pigskin quality, in 35～40 ℃ of backflow 5-8 hours, afterwards with distilled water flushing to free from extraneous odour, for subsequent use;
(3) above-mentioned pigskin is immersed in 0.6～0.7mol/L Glacial acetic acid and the pepsic mixed solution of 600～700mg/L, continue to stir about 25～30 hours;
(4) said mixture is adopted gradual ultrasonication, used first 100～200W supersound process 30 minutes, re-used 200～300W supersound process 30 minutes, used at last 300～400W supersound process 1 hour;
(5) H of adding 2～3%
2O
2Solution mixes and left standstill 2～4 hours, regulates pH to 5.0, and is centrifugal;
(6) get supernatant liquor, add NaCl, stirred 20-30 hour, centrifugal, throw out adds the dissolving of 0.6-0.7moL/L Glacial acetic acid, and is centrifugal, gets supernatant liquor, regulates pH to 7.5, adds NaCl, stirs 20-30 hour, centrifugal;
(7) throw out that step (6) is obtained is dissolved in 0.6～0.7moL/L Glacial acetic acid, first take the Glacial acetic acid of 0.6～0.7moL/L as extracellular fluid dialysis dialysis 2 times, each 4 hours, dialyse 5～7 times take distilled water as extracellular fluid dialysis again, each 4 hours, extremely outer liquid can't detect chlorion, obtains collagen liquid;
(8) ratio in 40-60U/g collagen adds glutamine transaminage, crosslinked 2-5 hour;
(9) lyophilize obtains collagen protein sponge.
The acid enzyme is in conjunction with processing: sour enzyme of the present invention refers to immerse in 0.6～0.7mol/L Glacial acetic acid and the pepsic mixed solution of 600～700mg/L through pretreated raw material in conjunction with processing, continues to stir about 25～30 hours.The method combines the characteristics that acid system and Enzymatic Extraction prepare collagen, has prevented from the destruction of active collagen triple-helix structure is effectively raised extraction efficiency.
Ultrasonication: sour enzyme of the present invention refers to adopt gradual ultrasonication in conjunction with processing, uses first 100～200W supersound process 30 minutes, re-uses 200～300W supersound process 30 minutes, uses at last 300～400W supersound process 1 hour.The present invention adopts ultrasonication combined acid Enzymatic Extraction to prepare collagen protein, through experimental studies have found that, adopts ultrasonication can effectively improve extraction efficiency.Adopt gradual ultrasonication to compare with adopting the constant power supersound process, can better prevent the destruction of the triple-helix structure of collagen, and effectively reduce the production energy consumption in ultrasonication stage.
Beneficial effect of the present invention: the present invention uses sour enzyme to add the preparation method of gradual ultrasonication in conjunction with processing, reduced to the full extent the destruction to collagen structure, the triple-helix structure that keeps active collagen, the extraction efficiency that the while has been improved collagen has significantly reduced production cost.Simultaneously, gradual ultrasonication has also reduced energy consumption to a certain extent in the ultrasonication link.The present invention uses H
2O
2Solution-treated acid adding dissolved salt is analysed purifying and is added the purity that the dialysis purifying effectively raises the collagen product.Use the crosslinked security that improves product of enzyme process.The collagen protein sponge product that method of the present invention makes has reduced immunogenicity and high-biocompatibility, by suitability for industrialized production, and is widely used in medical product, as: skin substitute products, bone surrogate, collagen protein dressing, biotechnology film etc.
Embodiment
Below in conjunction with embodiment, further set forth the present invention:
Embodiment 1
(1) get fresh ox-hide, cut and remove oil layer and trichocutis, remove impurity, clean, ox-hide is broken into fine granularity, according to 1: 30 ratio of solid-to-liquid ratio, add the aqueous sodium hydroxide solution of 0.03mol/L, in 6～8 ℃ of immersions 2 hours, filter, for subsequent use;
(2) adding quality in above-mentioned pretreated ox-hide is anhydrous diethyl ether or the acetone of 8 times of ox-hide quality, refluxed 8 hours in 35 ℃, afterwards with distilled water flushing to free from extraneous odour, for subsequent use;
(3) above-mentioned ox-hide is immersed in 0.6mol/L Glacial acetic acid and the pepsic mixed solution of 700mg/L, continue to stir about 25 hours;
(4) said mixture is adopted gradual ultrasonication, used first the 100W supersound process 30 minutes, re-used the 300W supersound process 30 minutes, used at last the 400W supersound process 1 hour;
(5) H of adding 2%
2O
2Solution mixes and left standstill 4 hours, regulates pH to 5.0, and is centrifugal;
(6) get supernatant liquor, add NaCl, stirred 20 hours, centrifugal, throw out adds the dissolving of 0.7moL/L Glacial acetic acid, and is centrifugal, gets supernatant liquor, regulates pH to 7.5, adds NaCl, stirs 20 hours, centrifugal;
(7) throw out that step (6) is obtained is dissolved in the 0.7moL/L Glacial acetic acid, first take the Glacial acetic acid of 0.7moL/L as extracellular fluid dialysis dialysis 2 times, each 4 hours, dialyse 5 times take distilled water as extracellular fluid dialysis again, each 4 hours, extremely outer liquid can't detect chlorion, obtains collagen liquid;
(8) ratio in 40U/g collagen adds glutamine transaminage, crosslinked 5 hours;
(9) lyophilize obtains collagen protein sponge.
Embodiment 2
(1) get fresh ox heel string, oil layer and trichocutis are removed in cutting, remove impurity, clean, the ox heel string is broken into fine granularity, according to 1: 40 ratio of solid-to-liquid ratio, adds the aqueous sodium hydroxide solution of 0.01mol/L, in 6～8 ℃ of immersions 1 hour, filter, for subsequent use;
(2) adding quality in above-mentioned pretreated ox heel string is anhydrous diethyl ether or the acetone of 6 times of ox heel string quality, refluxed 5 hours in 40 ℃, afterwards with distilled water flushing to free from extraneous odour, for subsequent use;
(3) above-mentioned ox heel string is immersed in 0.7mol/L Glacial acetic acid and the pepsic mixed solution of 600mg/L, continue to stir about 30 hours;
(4) said mixture is adopted gradual ultrasonication, used first the 100W supersound process 30 minutes, re-used the 200W supersound process 30 minutes, used at last the 300W supersound process 1 hour;
(5) H of adding 3%
2O
2Solution mixes and left standstill 2 hours, regulates pH to 5.0, and is centrifugal;
(6) get supernatant liquor, add NaCl, stirred 30 hours, centrifugal, throw out adds the dissolving of 0.6moL/L Glacial acetic acid, and is centrifugal, gets supernatant liquor, regulates pH to 7.5, adds NaCl, stirs 30 hours, centrifugal;
(7) throw out that step (6) is obtained is dissolved in the 0.6moL/L Glacial acetic acid, first take the Glacial acetic acid of 0.6moL/L as extracellular fluid dialysis dialysis 2 times, each 4 hours, dialyse 7 times take distilled water as extracellular fluid dialysis again, each 4 hours, extremely outer liquid can't detect chlorion, obtains collagen liquid;
(8) ratio in 60U/g collagen adds glutamine transaminage, crosslinked 2 hours;
(9) with the aforesaid liquid lyophilize, obtain collagen protein sponge.
Embodiment 3
(1) get fresh porcine skin, cut and remove oil layer and trichocutis, remove impurity, clean, pigskin is broken into fine granularity, according to 1: 35 ratio of solid-to-liquid ratio, add the aqueous sodium hydroxide solution of 0.02mol/L, in 6～8 ℃ of immersions 1.5 hours, filter, for subsequent use;
(2) adding quality in above-mentioned pretreated pigskin is anhydrous diethyl ether or the acetone of 7 times of pigskin quality, refluxed 6 hours in 37 ℃, afterwards with distilled water flushing to free from extraneous odour, for subsequent use;
(3) above-mentioned pigskin is immersed in 0.65mol/L Glacial acetic acid and the pepsic mixed solution of 650mg/L, continue to stir about 28 hours;
(4) said mixture is adopted gradual ultrasonication, used first the 150W supersound process 30 minutes, re-used the 250W supersound process 30 minutes, used at last the 350W supersound process 1 hour;
(5) H of adding 2.5%
2O
2Solution mixes and left standstill 3 hours, regulates pH to 5.0, and is centrifugal;
(6) get supernatant liquor, add NaCl, stirred 25 hours, centrifugal, throw out adds the dissolving of 0.65moL/L Glacial acetic acid, and is centrifugal, gets supernatant liquor, regulates pH to 7.5, adds NaCl, stirs 25 hours, centrifugal;
(7) throw out that step (6) is obtained is dissolved in the 0.65moL/L Glacial acetic acid, first take the Glacial acetic acid of 0.65moL/L as extracellular fluid dialysis dialysis 2 times, each 4 hours, dialyse 6 times take distilled water as extracellular fluid dialysis again, each 4 hours, extremely outer liquid can't detect chlorion, obtains collagen liquid;
(8) ratio in 50U/g collagen adds glutamine transaminage, crosslinked 4 hours;
(9) lyophilize obtains collagen protein sponge.
Claims (1)
1. the preparation method of a collagen protein sponge is characterized in that, comprises the steps:
(1) get fresh porcine skin, oil layer and trichocutis are removed in cutting, remove impurity, clean, pigskin is broken into fine granularity, according to 1: 30～1: 40 ratio of solid-to-liquid ratio, adds the aqueous sodium hydroxide solution of 0.01～0.03mol/L, in 6～8 ℃ of immersions 1～2 hour, filter, for subsequent use;
(2) adding quality in above-mentioned pretreated pigskin is anhydrous diethyl ether or the acetone of 6～8 times of pigskin quality, in 35～40 ℃ of backflow 5-8 hours, afterwards with distilled water flushing to free from extraneous odour, for subsequent use;
(3) above-mentioned pigskin is immersed in 0.6～0.7mol/L Glacial acetic acid and the pepsic mixed solution of 600～700mg/L, continue to stir 25～30 hours;
(4) said mixture is adopted gradual ultrasonication, used first 100～200W supersound process 30 minutes, re-used 200～300W supersound process 30 minutes, used at last 300～400W supersound process 1 hour;
(5) H of adding 2～3%
2O
2Solution mixes and left standstill 2～4 hours, regulates pH to 5.0, and is centrifugal;
(6) get supernatant liquor, add NaCl, stirred 20-30 hour, centrifugal, throw out adds the dissolving of 0.6-0.7moL/L Glacial acetic acid, and is centrifugal, gets supernatant liquor, regulates pH to 7.5, adds NaCl, stirs 20-30 hour, centrifugal;
(7) throw out that step (6) is obtained is dissolved in 0.6～0.7moL/L Glacial acetic acid, first take the Glacial acetic acid of 0.6～0.7moL/L as extracellular fluid dialysis dialysis 2 times, each 4 hours, dialyse 5～7 times take distilled water as extracellular fluid dialysis again, each 4 hours, extremely outer liquid can't detect chlorion, obtains collagen liquid;
(8) ratio in 40-60U/g collagen adds glutamine transaminage, crosslinked 2-5 hour;
(9) lyophilize obtains collagen protein sponge.
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| CN1032237C (en) * | 1991-10-11 | 1996-07-10 | 青岛海洋大学 | Biological material sponge producing process |
| CN1210019A (en) * | 1998-09-10 | 1999-03-10 | 战丽芬 | Medical collagen sponge and manufacture thereof |
| CN1179755C (en) * | 2001-11-09 | 2004-12-15 | 武继民 | Medical collagen sponge and its prepn |
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Address after: 214000, Jiangsu, Wuxi province Mashan biological medicine industry park (business place, Wuxi, Binhu District, Mashan, Mei Liang Road, No. 132) Patentee after: Wuxi Betty biological engineering Limited by Share Ltd Address before: 214063, Mashan biological pharmaceutical industry park (Ziyun Road), Binhu District, Jiangsu, Wuxi Province Patentee before: Wuxi Biot Bio-technology Co., Ltd. |