PL440931A1 - Pair of oligonucleotide primers for the detection and method of detecting the allele of the dominant crown rust resistance gene from the subline of the hybrid form A. sterilis PI 296244 x A. sativa TAM-O-312 in oat plants (Avena sativa L.) - Google Patents

Pair of oligonucleotide primers for the detection and method of detecting the allele of the dominant crown rust resistance gene from the subline of the hybrid form A. sterilis PI 296244 x A. sativa TAM-O-312 in oat plants (Avena sativa L.)

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Publication number
PL440931A1
PL440931A1 PL440931A PL44093122A PL440931A1 PL 440931 A1 PL440931 A1 PL 440931A1 PL 440931 A PL440931 A PL 440931A PL 44093122 A PL44093122 A PL 44093122A PL 440931 A1 PL440931 A1 PL 440931A1
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PL
Poland
Prior art keywords
sativa
pair
sterilis
subline
tam
Prior art date
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PL440931A
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Polish (pl)
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PL245140B1 (en
Inventor
Edyta Paczos-Grzęda
Sylwia Sowa
Joanna Toporowska
Aneta Koroluk
Krzysztof Kowalczyk
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Uniwersytet Przyrodniczy W Lublinie
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Application filed by Uniwersytet Przyrodniczy W Lublinie filed Critical Uniwersytet Przyrodniczy W Lublinie
Priority to PL440931A priority Critical patent/PL245140B1/en
Publication of PL440931A1 publication Critical patent/PL440931A1/en
Publication of PL245140B1 publication Critical patent/PL245140B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Botany (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Przedmiot zgłoszenia stanowi para oligonukleotydowych starterów do wykrywania allelu dominującego genu odporności na rdzę koronową z sublinii formy A. sterilis PI 296244 x A. sativa TAM-O-312 w roślinach owsa o sekwencjach nr 1 i 2 przedstawionych na liście sekwencji. Przedmiotem zgłoszenia jest ponadto sposób identyfikacji allelu dominującego genu odporności na rdzę koronową z sublinii formy mieszańcowej A. sterilis PI 296244 x A. sativa TAM-O-312 w roślinach owsa, w którym polimorficzny fragment DNA sprzężony z badanym genem amplifikowany jest w reakcji PCR z zastosowaniem pary starterów, po czym dokonuje się detekcji produktu amplifikacji, a parę starterów stanowi para oligonukleotydowych starterów o sekwencjach nr 1 i 2 przedstawionych na liście sekwencji, przy czym stosuje się marker 821 przedstawiony na Fig. 1 - 3. W wyniku PCR amplifikowany jest fragment DNA o długości 61 par zasad o sekwencji nr 3 przedstawionej na liście sekwencji.The subject of the application is a pair of oligonucleotide primers for detecting the dominant allele of the crown rust resistance gene from the subline of the form A. sterilis PI 296244 x A. sativa TAM-O-312 in oat plants with sequences no. 1 and 2 presented in the sequence list. The subject of the application is also a method of identifying the allele of the dominant gene for resistance to crown rust from the subline of the hybrid form A. sterilis PI 296244 x A. sativa TAM-O-312 in oat plants, in which a polymorphic DNA fragment linked to the tested gene is amplified in a PCR reaction with using a pair of primers, after which the amplification product is detected, and the pair of primers is a pair of oligonucleotide primers with sequences No. 1 and 2 presented in the sequence list, using the marker 821 shown in Fig. 1 - 3. As a result of PCR, a fragment is amplified 61 base pair DNA with sequence number 3 shown in the sequence list.

PL440931A 2022-04-13 2022-04-13 Pair of oligonucleotide primers for the detection and method of detecting the allele of the dominant crown rust resistance gene from the subline of the hybrid form A. sterilis PI 296244 x A. sativa TAM-O-312 in oat plants (Avena sativa L.) PL245140B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL440931A PL245140B1 (en) 2022-04-13 2022-04-13 Pair of oligonucleotide primers for the detection and method of detecting the allele of the dominant crown rust resistance gene from the subline of the hybrid form A. sterilis PI 296244 x A. sativa TAM-O-312 in oat plants (Avena sativa L.)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL440931A PL245140B1 (en) 2022-04-13 2022-04-13 Pair of oligonucleotide primers for the detection and method of detecting the allele of the dominant crown rust resistance gene from the subline of the hybrid form A. sterilis PI 296244 x A. sativa TAM-O-312 in oat plants (Avena sativa L.)

Publications (2)

Publication Number Publication Date
PL440931A1 true PL440931A1 (en) 2023-10-16
PL245140B1 PL245140B1 (en) 2024-05-20

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PL440931A PL245140B1 (en) 2022-04-13 2022-04-13 Pair of oligonucleotide primers for the detection and method of detecting the allele of the dominant crown rust resistance gene from the subline of the hybrid form A. sterilis PI 296244 x A. sativa TAM-O-312 in oat plants (Avena sativa L.)

Country Status (1)

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Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL233477B1 (en) * 2017-08-11 2019-10-31 Univ Przyrodniczy W Lublinie A pair of starter oligonucleotides for detecting, and the method for detecting of the recessive allele of immunity gene Pc39 to crown rust in the plants of common oat (Avena sativa L.)
PL233478B1 (en) * 2017-08-11 2019-10-31 Univ Przyrodniczy W Lublinie A pair of starter oligonucleotides for detecting, and the method for detecting of the dominant allele of immunity gene to crown rust Pc39 in the plants of common oat (Avena sativa L.)
PL233476B1 (en) * 2017-08-11 2019-10-31 Univ Przyrodniczy W Lublinie Two pairs of starter oligonucleotides for detecting the presence of alleles of the dominating or recessive immunity gene to crown rust Pc39 in the genome of common oat (Avena sativa L.), combination of two starter pairs and method for detecting of the of gene Pc39 alleles system

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Publication number Publication date
PL245140B1 (en) 2024-05-20

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