PL436922A1 - Sposób pozyskiwania nieembrionalnych, mezenchymalnych komórek macierzystych i egzosomów z dziąsła oraz krioprzechowywania komórek macierzystych, dziąsła i egzosomów - Google Patents

Sposób pozyskiwania nieembrionalnych, mezenchymalnych komórek macierzystych i egzosomów z dziąsła oraz krioprzechowywania komórek macierzystych, dziąsła i egzosomów

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Publication number
PL436922A1
PL436922A1 PL436922A PL43692221A PL436922A1 PL 436922 A1 PL436922 A1 PL 436922A1 PL 436922 A PL436922 A PL 436922A PL 43692221 A PL43692221 A PL 43692221A PL 436922 A1 PL436922 A1 PL 436922A1
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Poland
Prior art keywords
exosomes
stem cells
gingiva
cryo
storage
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PL436922A
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English (en)
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PL243367B1 (pl
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Zuzanna Stern
Katarzyna Stefańska
Marta Dyszkiewicz-Korwinska
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Cellivia 3 Spółka Akcyjna
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Priority to PL436922A priority Critical patent/PL243367B1/pl
Publication of PL436922A1 publication Critical patent/PL436922A1/pl
Publication of PL243367B1 publication Critical patent/PL243367B1/pl

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0278—Physical preservation processes
    • A01N1/0284—Temperature processes, i.e. using a designated change in temperature over time
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0654—Osteocytes, Osteoblasts, Odontocytes; Bones, Teeth
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0664—Dental pulp stem cells, Dental follicle stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
    • C12N2506/1346—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells
    • C12N2506/1361—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells from dental pulp or dental follicle stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2523/00—Culture process characterised by temperature

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Rheumatology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Wynalazek dotyczy sposobu pozyskiwania nieembrionalnych komórek macierzystych i egzosomów z tkanki dziąsła i ich przechowywania w oparach wrzącego ciekłego azotu. Uwolnione komórki macierzyste po namnożeniu in vitro, lub całe fragmenty dziąseł pozyskiwanych w trakcie ekstrakcji zęba z powodów stomatologicznych lub ortodoncji są przechowywane w nieograniczonym czasie w oparach wrzącego płynnego azotu o temperaturze -196°C. Multipotentne komórki macierzyńskie pozyskane z dziąsła po namnożeniu mogą być w różnorakich kompozycjach wykorzystywane w leczeniu i rekonstrukcji tkanki zębów, jamy ustnej,  zaś egzosomy wykorzystane do przenoszenia materiału biologicznego do innych komórek, między innymi w procesach leczenia.
PL436922A 2021-02-10 2021-02-10 Sposób krioprzechowywania nieembrionalnych, mezenchymalnych komórek macierzystych z dziąsła PL243367B1 (pl)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL436922A PL243367B1 (pl) 2021-02-10 2021-02-10 Sposób krioprzechowywania nieembrionalnych, mezenchymalnych komórek macierzystych z dziąsła

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL436922A PL243367B1 (pl) 2021-02-10 2021-02-10 Sposób krioprzechowywania nieembrionalnych, mezenchymalnych komórek macierzystych z dziąsła

Publications (2)

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PL436922A1 true PL436922A1 (pl) 2022-08-16
PL243367B1 PL243367B1 (pl) 2023-08-14

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PL436922A PL243367B1 (pl) 2021-02-10 2021-02-10 Sposób krioprzechowywania nieembrionalnych, mezenchymalnych komórek macierzystych z dziąsła

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PL243367B1 (pl) 2023-08-14

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