PL434484A1 - Zastosowanie fuzyjnej polimerazy DNA Bst-Nec do izotermalnego powielania specyficznych sekwencji wirusa SARS CoV-2 - Google Patents

Zastosowanie fuzyjnej polimerazy DNA Bst-Nec do izotermalnego powielania specyficznych sekwencji wirusa SARS CoV-2

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Publication number
PL434484A1
PL434484A1 PL434484A PL43448420A PL434484A1 PL 434484 A1 PL434484 A1 PL 434484A1 PL 434484 A PL434484 A PL 434484A PL 43448420 A PL43448420 A PL 43448420A PL 434484 A1 PL434484 A1 PL 434484A1
Authority
PL
Poland
Prior art keywords
bst
nec
polymerase
isometric
reproduction
Prior art date
Application number
PL434484A
Other languages
English (en)
Other versions
PL241065B1 (pl
Inventor
Marta SKWARECKA
Kasjan SZEMIAKO
Dawid NIDZWORSKI
Sabina ŻOŁĘDOWSKA
Marcin Olszewski
Original Assignee
Geneme Spółka Z Ograniczoną Odpowiedzialnością
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Geneme Spółka Z Ograniczoną Odpowiedzialnością filed Critical Geneme Spółka Z Ograniczoną Odpowiedzialnością
Priority to PL434484A priority Critical patent/PL241065B1/pl
Priority to PCT/PL2020/000074 priority patent/WO2021262013A1/en
Publication of PL434484A1 publication Critical patent/PL434484A1/pl
Publication of PL241065B1 publication Critical patent/PL241065B1/pl

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Przedmiotem zgłoszenia jest fuzyjna polimeraza DNA Bst-Nec do zastosowania do izotermalnego powielania specyficznych sekwencji wirusa SARS CoV-2, charakteryzująca się tym, że wykrywa RNA wirusa z próbki materiału biologicznego bez izolacji RNA.
PL434484A 2020-06-26 2020-06-26 Zastosowanie fuzyjnej polimerazy DNA Bst-Nec do izotermalnego powielania specyficznych sekwencji wirusa SARS CoV-2 PL241065B1 (pl)

Priority Applications (2)

Application Number Priority Date Filing Date Title
PL434484A PL241065B1 (pl) 2020-06-26 2020-06-26 Zastosowanie fuzyjnej polimerazy DNA Bst-Nec do izotermalnego powielania specyficznych sekwencji wirusa SARS CoV-2
PCT/PL2020/000074 WO2021262013A1 (en) 2020-06-26 2020-08-31 Bst-nec dna fusion polymerase for use in isothermal replication of specific sars cov-2 virus sequences

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL434484A PL241065B1 (pl) 2020-06-26 2020-06-26 Zastosowanie fuzyjnej polimerazy DNA Bst-Nec do izotermalnego powielania specyficznych sekwencji wirusa SARS CoV-2

Publications (2)

Publication Number Publication Date
PL434484A1 true PL434484A1 (pl) 2021-12-27
PL241065B1 PL241065B1 (pl) 2022-08-01

Family

ID=72709833

Family Applications (1)

Application Number Title Priority Date Filing Date
PL434484A PL241065B1 (pl) 2020-06-26 2020-06-26 Zastosowanie fuzyjnej polimerazy DNA Bst-Nec do izotermalnego powielania specyficznych sekwencji wirusa SARS CoV-2

Country Status (2)

Country Link
PL (1) PL241065B1 (pl)
WO (1) WO2021262013A1 (pl)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL241698B1 (pl) * 2021-01-27 2022-11-21 Inst Biotechnologii I Medycyny Molekularnej Polimeraza Pwo-NeqSSB, sposób jej otrzymywania, plazmid rekombinantowy, startery oraz zastosowanie polimerazy

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070059713A1 (en) * 2005-09-09 2007-03-15 Lee Jun E SSB-DNA polymerase fusion proteins
PL426093A1 (pl) * 2018-06-27 2020-01-02 Instytut Biotechnologii I Medycyny Molekularnej Fuzyjna polimeraza kwasu jednołańcuchowego DNA Bst, cząsteczka kwasu nukleinowego kodująca fuzyjną polimerazę DNA NeqSSB-Bst, sposób jej otrzymywania oraz zastosowanie

Also Published As

Publication number Publication date
PL241065B1 (pl) 2022-08-01
WO2021262013A1 (en) 2021-12-30

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