Instytut Katalizy I Fizykochemii Powierzchni Im. Jerzego Habera Polskiej Akademii Nauk
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Preparation Of Compounds By Using Micro-Organisms
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Abstract
Sposób biokatalitycznego otrzymywania 25-hydroksylowanej witaminy D3 (kalcyfediolu) o wzorze II, polega na tym, że prowadzi się proces regioselektywnej hydroksylacji substratu o wzorze I w środowisku wodno - organicznym, w obecności reutleniacza, w atmosferze beztlenowej, w temperaturze 15 - 45°C, a zwłaszcza do 30°C, z użyciem preparatu enzymatycznego, wykazującego aktywność dehydrogenazy C25 steroidowej (S25DH), pochodzącej z bakterii Sterolibacterium denitrificans Chol-1S.The method of biocatalytic preparation of 25-hydroxylated vitamin D3 (calciphediol) of formula II consists in the process of regioselective hydroxylation of the substrate of formula I in a water-organic environment, in the presence of a re-oxidiser, in an anaerobic atmosphere at a temperature of 15 - 45 ° C and especially up to 30 ° C, using an enzyme preparation having C25 steroid dehydrogenase (S25DH) activity derived from the bacterium Sterolibacterium denitrificans Chol-1S.
PL415451A2015-12-222015-12-22Method for obtaining 25-hydroxylated vitamin D3 (calcifediol)
PL235932B1
(en)
active composition at ambient conditions for stain removal from stained substrates, pretreatment device, process for removing a stain from a stained substrate and use of an arginine alkyl ester compound