PL400718A1 - Helper plasmid, the strain and the system of the broad host spectrum plasmid mobilization and use thereof - Google Patents

Helper plasmid, the strain and the system of the broad host spectrum plasmid mobilization and use thereof

Info

Publication number
PL400718A1
PL400718A1 PL400718A PL40071812A PL400718A1 PL 400718 A1 PL400718 A1 PL 400718A1 PL 400718 A PL400718 A PL 400718A PL 40071812 A PL40071812 A PL 40071812A PL 400718 A1 PL400718 A1 PL 400718A1
Authority
PL
Poland
Prior art keywords
plasmid
mobilization
strain
broad host
host spectrum
Prior art date
Application number
PL400718A
Other languages
Polish (pl)
Other versions
PL230884B1 (en
Inventor
Michal Dmowski
Izabela Kern-Zdanowicz
Original Assignee
Instytut Biochemii I Biofizyki Polskiej Akademii Nauk
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Instytut Biochemii I Biofizyki Polskiej Akademii Nauk filed Critical Instytut Biochemii I Biofizyki Polskiej Akademii Nauk
Priority to PL400718A priority Critical patent/PL230884B1/en
Priority to PCT/IB2013/058368 priority patent/WO2014037917A1/en
Publication of PL400718A1 publication Critical patent/PL400718A1/en
Publication of PL230884B1 publication Critical patent/PL230884B1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)

Abstract

Wynalazek dotyczy plazmidu pomocniczego do mobilizacji, który obejmuje część mobilizującą systemu koniugacyjnego plazmidu pCTX-M3 (geny regionów tra i trb) oraz szczepu bakteryjnego do wydajnej mobilizacji plazmidów o szerokim spektrum gospodarzy zawierającego wintegrowaną w chromosom bakteryjny część mobilizującą systemu koniugacyjnego (geny regionów tra i trb). Wynalazek dotyczy również systemów do wydajnej mobilizacji plazmidów o szerokim spektrum gospodarzy obejmujących ten plazmid albo szczep bakteryjny oraz plazmid mobilizowany zawierający oriT o sekwencji nukleotydowej kompatybilnej z tym systemem.The invention relates to a mobilization helper plasmid which includes the mobilizing part of the pCTX-M3 conjugation system (tra and trb region genes) and a bacterial strain for efficient mobilization of broad host spectrum plasmids comprising the mobilizing part of the conjugation system (tra and trb region genes) integrated into the bacterial chromosome ). The invention also relates to systems for the efficient mobilization of plasmids with a broad spectrum of hosts comprising said plasmid or bacterial strain and a mobilized plasmid containing oriT having a nucleotide sequence compatible with said system.

PL400718A 2012-09-10 2012-09-10 Helper plasmid, the strain and the system of the broad host spectrum plasmid mobilization and use thereof PL230884B1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
PL400718A PL230884B1 (en) 2012-09-10 2012-09-10 Helper plasmid, the strain and the system of the broad host spectrum plasmid mobilization and use thereof
PCT/IB2013/058368 WO2014037917A1 (en) 2012-09-10 2013-09-07 The helper plasmid, bacterial strain and the broad host range system for plasmid mobilization and uses thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL400718A PL230884B1 (en) 2012-09-10 2012-09-10 Helper plasmid, the strain and the system of the broad host spectrum plasmid mobilization and use thereof

Publications (2)

Publication Number Publication Date
PL400718A1 true PL400718A1 (en) 2014-03-17
PL230884B1 PL230884B1 (en) 2018-12-31

Family

ID=49724617

Family Applications (1)

Application Number Title Priority Date Filing Date
PL400718A PL230884B1 (en) 2012-09-10 2012-09-10 Helper plasmid, the strain and the system of the broad host spectrum plasmid mobilization and use thereof

Country Status (2)

Country Link
PL (1) PL230884B1 (en)
WO (1) WO2014037917A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR3099769B1 (en) 2019-08-05 2021-11-05 Bgene Genetics Bacterial mutagenesis by conjugation in liquid medium
WO2023092189A1 (en) * 2021-11-25 2023-06-01 The Westmead Institute for Medical Research Broad host range conjugative plasmids
CN114874968A (en) * 2022-06-21 2022-08-09 中国林业科学研究院森林生态环境与自然保护研究所 Method for in-situ modification of metagenome of plant endophytic microbiome

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6991786B1 (en) * 2000-08-30 2006-01-31 Wisconsin Alumni Research Foundation Anti-microbial biotherapeutic agents: alternatives to conventional pharmaceutical antibiotics

Also Published As

Publication number Publication date
PL230884B1 (en) 2018-12-31
WO2014037917A1 (en) 2014-03-13

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