PL400598A1 - Method for of identification of Gram-negative bacteria using the MALDI-TOF MS method - Google Patents

Method for of identification of Gram-negative bacteria using the MALDI-TOF MS method

Info

Publication number
PL400598A1
PL400598A1 PL400598A PL40059812A PL400598A1 PL 400598 A1 PL400598 A1 PL 400598A1 PL 400598 A PL400598 A PL 400598A PL 40059812 A PL40059812 A PL 40059812A PL 400598 A1 PL400598 A1 PL 400598A1
Authority
PL
Poland
Prior art keywords
maldi
gram
tof
negative bacteria
identification
Prior art date
Application number
PL400598A
Other languages
Polish (pl)
Other versions
PL223203B1 (en
Inventor
Sabina Koj
Tomasz Niedziela
Czesław Ługowski
Original Assignee
Wroclawskie Centrum Badan Eit + Spólka Z Ograniczona Odpowiedzialnoscia
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wroclawskie Centrum Badan Eit + Spólka Z Ograniczona Odpowiedzialnoscia filed Critical Wroclawskie Centrum Badan Eit + Spólka Z Ograniczona Odpowiedzialnoscia
Priority to PL400598A priority Critical patent/PL223203B1/en
Priority to PCT/PL2013/000112 priority patent/WO2014035270A1/en
Publication of PL400598A1 publication Critical patent/PL400598A1/en
Publication of PL223203B1 publication Critical patent/PL223203B1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor

Abstract

Przedmiotem wynalazku jest sposób identyfikacji chemotypów lipopolisacharydowych bakterii Gram-ujemnych w oparciu o profil endotoksynowy pelnych komórek bakteryjnych uzyskany z zastosowaniem metody MALDI-TOF MS. Profil pochodzacy od lipopolisacharydów (LPS) dla preparatów mas bakteryjnych uzyskuje sie w trybie jonizacji ujemnej spektrometru MALDI-TOF z zastosowaniem DHB jako matrycy. Przedmiotowe rozwiazanie moze sluzyc do analizy próbek klinicznych, diagnozy infekcji powodowanych przez bakterie Gram-ujemne oraz miec zastosowanie jako narzedzie w terapii nakierowanej na antygen O z pomoca swoistych przeciwcial.The subject of the invention is a method of identifying lipopolysaccharide chemotypes of gram-negative bacteria based on the endotoxin profile of whole bacterial cells obtained using the MALDI-TOF MS method. The lipopolysaccharide (LPS) profile for bacterial mass preparations is obtained in the negative ionization mode of the MALDI-TOF spectrometer using DHB as the matrix. This solution can be used to analyze clinical samples, diagnose infections caused by Gram-negative bacteria, and be used as a tool in therapy directed towards the O antigen with the help of specific antibodies.

PL400598A 2012-08-31 2012-08-31 Method for of identification of Gram-negative bacteria using the MALDI-TOF MS method PL223203B1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
PL400598A PL223203B1 (en) 2012-08-31 2012-08-31 Method for of identification of Gram-negative bacteria using the MALDI-TOF MS method
PCT/PL2013/000112 WO2014035270A1 (en) 2012-08-31 2013-08-30 A method of identifying gram-negative bacteria using the maldi-tof ms method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL400598A PL223203B1 (en) 2012-08-31 2012-08-31 Method for of identification of Gram-negative bacteria using the MALDI-TOF MS method

Publications (2)

Publication Number Publication Date
PL400598A1 true PL400598A1 (en) 2014-03-03
PL223203B1 PL223203B1 (en) 2016-10-31

Family

ID=49510477

Family Applications (1)

Application Number Title Priority Date Filing Date
PL400598A PL223203B1 (en) 2012-08-31 2012-08-31 Method for of identification of Gram-negative bacteria using the MALDI-TOF MS method

Country Status (2)

Country Link
PL (1) PL223203B1 (en)
WO (1) WO2014035270A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9273339B2 (en) 2011-01-03 2016-03-01 University Of Maryland, Baltimore Methods for identifying bacteria
PL404247A1 (en) 2013-06-07 2014-12-08 Wrocławskie Centrum Badań Eit + Spółka Z Ograniczoną Odpowiedzialnością LOS oligosugar Bordetella pertussis and pertussis toxin and its use in the prevention and treatment of infections caused by Bordetella pertussis
ES2696757R1 (en) * 2014-05-30 2019-01-21 Csir Method and device for detection of whole bacteria

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5059527A (en) * 1989-12-08 1991-10-22 White David C Detection of endotoxins of gram negative bacteria
US6177266B1 (en) 1996-04-29 2001-01-23 The United States Of America As Represented By The Secretary Of The Army Rapid identification of bacteria by mass spectrometry
US7684934B2 (en) 2003-06-06 2010-03-23 The United States Of America As Represented By The Department Of Health And Human Services Pattern recognition of whole cell mass spectra
EP1942194A1 (en) 2007-01-08 2008-07-09 Université René Descartes Method for identifying a germ isolated from a clinical sample
US9273339B2 (en) * 2011-01-03 2016-03-01 University Of Maryland, Baltimore Methods for identifying bacteria

Also Published As

Publication number Publication date
PL223203B1 (en) 2016-10-31
WO2014035270A1 (en) 2014-03-06
WO2014035270A8 (en) 2015-02-12

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