WO2014035270A8 - A method of identifying gram-negative bacteria using the maldi-tof ms method - Google Patents

A method of identifying gram-negative bacteria using the maldi-tof ms method Download PDF

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Publication number
WO2014035270A8
WO2014035270A8 PCT/PL2013/000112 PL2013000112W WO2014035270A8 WO 2014035270 A8 WO2014035270 A8 WO 2014035270A8 PL 2013000112 W PL2013000112 W PL 2013000112W WO 2014035270 A8 WO2014035270 A8 WO 2014035270A8
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WO
WIPO (PCT)
Prior art keywords
maldi
tof
negative bacteria
gram
useful
Prior art date
Application number
PCT/PL2013/000112
Other languages
French (fr)
Other versions
WO2014035270A1 (en
Inventor
Sabina KOJ
Tomasz Niedziela
Czesław ŁUGOWSKI
Original Assignee
Wrocławskie Centrum Badań Eit + Spółka Z Organiczoną Odpowiedzialnością
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wrocławskie Centrum Badań Eit + Spółka Z Organiczoną Odpowiedzialnością filed Critical Wrocławskie Centrum Badań Eit + Spółka Z Organiczoną Odpowiedzialnością
Publication of WO2014035270A1 publication Critical patent/WO2014035270A1/en
Publication of WO2014035270A8 publication Critical patent/WO2014035270A8/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor

Abstract

The present invention relates to the identification of lipopolysaccharide chemotypes of Gram-negative bacteria based on the endotoxin profile of whole bacteria using MALDI-TOF MS. The profile derived from lipopolysaccharides (LPS) for bacterial preparation is obtained in the negative mode of a MALDI-TOF spectrometer using DHB as a matrix. The subject invention is useful in the analysis of clinical samples, diagnosis of infections caused by Gram-negative bacteria and can be useful as a tool in the therapy directed against the O-antigen using specific antibodies.
PCT/PL2013/000112 2012-08-31 2013-08-30 A method of identifying gram-negative bacteria using the maldi-tof ms method WO2014035270A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PL400598A PL223203B1 (en) 2012-08-31 2012-08-31 Method for of identification of Gram-negative bacteria using the MALDI-TOF MS method
PLP.400598 2012-08-31

Publications (2)

Publication Number Publication Date
WO2014035270A1 WO2014035270A1 (en) 2014-03-06
WO2014035270A8 true WO2014035270A8 (en) 2015-02-12

Family

ID=49510477

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/PL2013/000112 WO2014035270A1 (en) 2012-08-31 2013-08-30 A method of identifying gram-negative bacteria using the maldi-tof ms method

Country Status (2)

Country Link
PL (1) PL223203B1 (en)
WO (1) WO2014035270A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9273339B2 (en) 2011-01-03 2016-03-01 University Of Maryland, Baltimore Methods for identifying bacteria
PL404247A1 (en) 2013-06-07 2014-12-08 Wrocławskie Centrum Badań Eit + Spółka Z Ograniczoną Odpowiedzialnością LOS oligosugar Bordetella pertussis and pertussis toxin and its use in the prevention and treatment of infections caused by Bordetella pertussis
ES2696757R1 (en) * 2014-05-30 2019-01-21 Csir Method and device for detection of whole bacteria

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5059527A (en) * 1989-12-08 1991-10-22 White David C Detection of endotoxins of gram negative bacteria
US6177266B1 (en) 1996-04-29 2001-01-23 The United States Of America As Represented By The Secretary Of The Army Rapid identification of bacteria by mass spectrometry
US7684934B2 (en) 2003-06-06 2010-03-23 The United States Of America As Represented By The Department Of Health And Human Services Pattern recognition of whole cell mass spectra
EP1942194A1 (en) 2007-01-08 2008-07-09 Université René Descartes Method for identifying a germ isolated from a clinical sample
US9273339B2 (en) * 2011-01-03 2016-03-01 University Of Maryland, Baltimore Methods for identifying bacteria

Also Published As

Publication number Publication date
PL223203B1 (en) 2016-10-31
WO2014035270A1 (en) 2014-03-06
PL400598A1 (en) 2014-03-03

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