PL397240A1 - Sposób detekcji S-glikozylowanych bialek i peptydów - Google Patents
Sposób detekcji S-glikozylowanych bialek i peptydówInfo
- Publication number
- PL397240A1 PL397240A1 PL397240A PL39724011A PL397240A1 PL 397240 A1 PL397240 A1 PL 397240A1 PL 397240 A PL397240 A PL 397240A PL 39724011 A PL39724011 A PL 39724011A PL 397240 A1 PL397240 A1 PL 397240A1
- Authority
- PL
- Poland
- Prior art keywords
- amino acid
- glycosylated
- compounds
- peptides
- proteins
- Prior art date
Links
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 4
- 238000001514 detection method Methods 0.000 title abstract 3
- 102000004196 processed proteins & peptides Human genes 0.000 title abstract 3
- 108091007464 S-glycosylated proteins Proteins 0.000 title abstract 2
- 150000001875 compounds Chemical class 0.000 abstract 3
- 238000000034 method Methods 0.000 abstract 2
- 238000012986 modification Methods 0.000 abstract 2
- 230000004048 modification Effects 0.000 abstract 2
- 125000001433 C-terminal amino-acid group Chemical group 0.000 abstract 1
- 125000001429 N-terminal alpha-amino-acid group Chemical group 0.000 abstract 1
- 239000002253 acid Substances 0.000 abstract 1
- 235000001014 amino acid Nutrition 0.000 abstract 1
- 125000000539 amino acid group Chemical group 0.000 abstract 1
- 150000001413 amino acids Chemical class 0.000 abstract 1
- 230000000903 blocking effect Effects 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 abstract 1
- 150000002243 furanoses Chemical group 0.000 abstract 1
- 238000006206 glycosylation reaction Methods 0.000 abstract 1
- 230000007062 hydrolysis Effects 0.000 abstract 1
- 238000006460 hydrolysis reaction Methods 0.000 abstract 1
- 150000002772 monosaccharides Chemical class 0.000 abstract 1
- 229920001542 oligosaccharide Polymers 0.000 abstract 1
- -1 oligosaccharide glycan Chemical class 0.000 abstract 1
- 230000001323 posttranslational effect Effects 0.000 abstract 1
- 235000018102 proteins Nutrition 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 150000003215 pyranoses Chemical class 0.000 abstract 1
- 230000003595 spectral effect Effects 0.000 abstract 1
- 238000001228 spectrum Methods 0.000 abstract 1
- 238000004885 tandem mass spectrometry Methods 0.000 abstract 1
- 125000003396 thiol group Chemical group [H]S* 0.000 abstract 1
Landscapes
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
Sposób detekcji S-glikozylowanych bialek i peptydów wsród struktur o ogólnym wzorze A1A2...[An]...Az, w którym A1, A2, An, Az oznaczaja reszty aminokwasowe, w tym reszty niosace syntetyczne lub naturalne potranslacyjne modyfikacje PTM, przy czym A1 oznacza aminokwas N-terminalny, Az - aminokwas C-terminalny, zas An oznacza dowolny aminokwas wewnetrzny, w tym S-glikozylowana reszte cysteinowa CG o strukturze przedstawionej we wzorze 2, w którym A1, A2, An i Az maja wyzej podane znaczenie, zas G oznacza poszukiwany nieznany, naturalny monosacharydowy lub oligosacharydowy glikan laczacy sie z lancuchem peptydowym wiazaniem S-glikozydowym, przy czym cukier laczacy moze miec strukture piranozowa lub furanozowa; reszta CG moze wystepowac w lancuchu jednokrotnie lub wielokrotnie, a takze zajmowac pozycje terminalne I i z, polega na tym, ze próbe bialek lub peptydów z pro-SH modyfikacjami uprzednio zastapionymi permanentnymi grupami blokujacymi, poddaje sie kolejno hydrolizie wiazan S-glikozydowych promowanej metalicznym tiofilowym kwasem Lewisa, demetalacji prowadzacej do uwolnienia grup tiolowych w miejscach S-glikozylacji, które nastepnie znakuje sie wybranym tiospecyficznym odczynnikiem w znany sposób i uzyskane S-znakowane zwiazki wykrywa sie metodami tandemowej spektrometrii masowej, a z otrzymanych tandemowych widm tych zwiazków konstruuje sie zastepcza biblioteke spektralna sluzaca do bezposredniego wykrywania S-glikozylowanych zwiazków.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL397240A PL226828B1 (pl) | 2011-12-05 | 2011-12-05 | Sposób detekcji S-glikozylowanych białek ipeptydów |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL397240A PL226828B1 (pl) | 2011-12-05 | 2011-12-05 | Sposób detekcji S-glikozylowanych białek ipeptydów |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| PL397240A1 true PL397240A1 (pl) | 2013-06-10 |
| PL226828B1 PL226828B1 (pl) | 2017-09-29 |
Family
ID=48539559
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PL397240A PL226828B1 (pl) | 2011-12-05 | 2011-12-05 | Sposób detekcji S-glikozylowanych białek ipeptydów |
Country Status (1)
| Country | Link |
|---|---|
| PL (1) | PL226828B1 (pl) |
-
2011
- 2011-12-05 PL PL397240A patent/PL226828B1/pl unknown
Also Published As
| Publication number | Publication date |
|---|---|
| PL226828B1 (pl) | 2017-09-29 |
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