PL396026A1 - Process for the preparation of proteins with immunoregulatory properties - Google Patents

Process for the preparation of proteins with immunoregulatory properties

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Publication number
PL396026A1
PL396026A1 PL396026A PL39602611A PL396026A1 PL 396026 A1 PL396026 A1 PL 396026A1 PL 396026 A PL396026 A PL 396026A PL 39602611 A PL39602611 A PL 39602611A PL 396026 A1 PL396026 A1 PL 396026A1
Authority
PL
Poland
Prior art keywords
kda
permeability
membranes
ultrafiltration
limit
Prior art date
Application number
PL396026A
Other languages
Polish (pl)
Other versions
PL218076B1 (en
Inventor
Antoni Polanowski
Tadeusz Trziszka
Agnieszka Sosnowska
Original Assignee
Uniwersytet Przyrodniczy we Wrocławiu
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Uniwersytet Przyrodniczy we Wrocławiu filed Critical Uniwersytet Przyrodniczy we Wrocławiu
Priority to PL396026A priority Critical patent/PL218076B1/en
Publication of PL396026A1 publication Critical patent/PL396026A1/en
Publication of PL218076B1 publication Critical patent/PL218076B1/en

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  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Sposób polega na tym, że immunoglobulinę Y, poddaje się dializie wobec buforu fosforanowego, po czym dializat klaruje się i zagęszcza na membranie o przepuszczalności granicznej poniżej 3 kDa, a otrzymany retentat poddaje się chromatografii sitowo-molekularnej zbierając grupę niskocząsteczkowych białek oddzielonych od immunoglobuliny Y, zwaną Yolkiną. Alternatywnie dializat poddaje się ultrafiltracji przez membrany o przepuszczalności granicznej poniżej 50 kDa, po czym permeat zagęszcza się ultrafiltrując przez membrany o przepuszczalności granicznej poniżej 3 kDa. Następnie frakcje białek zwolnione z kompleksu z IgY, poddaje się dializie do wody przez błony o przepuszczalności granicznej poniżej 3 kDa w temperaturze w warunkach chłodniczych przez okres co najmniej 24 godzin z kilkukrotną zmianą wody. Alternatywnie frakcje białek odsala się z użyciem modułów ultrafiltracyjnych o przepuszczalności granicznej 1 kDa i poddaje suszeniu, uzyskując preparat o właściwościach immunoregulatorowych. Wynalazek może znaleźć zastosowanie w przemyśle spożywczym i farmaceutycznym, do produkcji nutraceutyków i produktów farmakologicznych.The method consists in dialysis of immunoglobulin Y against a phosphate buffer, then the dialysate is clarified and concentrated on a membrane with a limit permeability below 3 kDa, and the obtained retentate is subjected to molecular sieve chromatography, collecting a group of low molecular weight proteins separated from immunoglobulin Y, called Yolkina. Alternatively, the dialysate is subjected to ultrafiltration through membranes with an intrinsic permeability of less than 50 kDa, and the permeate is then concentrated by ultrafiltration through membranes with an intrinsic permeability of less than 3 kDa. Then, the protein fractions released from the complex with IgY are dialyzed into water through membranes with a limit permeability below 3 kDa at refrigerated temperature for a period of at least 24 hours with several water changes. Alternatively, protein fractions are desalted using ultrafiltration modules with a permeability limit of 1 kDa and dried, obtaining a preparation with immunoregulatory properties. The invention may be used in the food and pharmaceutical industries, for the production of nutraceuticals and pharmacological products.

PL396026A 2011-08-19 2011-08-19 Process for the preparation of proteins with immunoregulatory properties PL218076B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL396026A PL218076B1 (en) 2011-08-19 2011-08-19 Process for the preparation of proteins with immunoregulatory properties

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL396026A PL218076B1 (en) 2011-08-19 2011-08-19 Process for the preparation of proteins with immunoregulatory properties

Publications (2)

Publication Number Publication Date
PL396026A1 true PL396026A1 (en) 2012-01-30
PL218076B1 PL218076B1 (en) 2014-10-31

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ID=45510334

Family Applications (1)

Application Number Title Priority Date Filing Date
PL396026A PL218076B1 (en) 2011-08-19 2011-08-19 Process for the preparation of proteins with immunoregulatory properties

Country Status (1)

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PL (1) PL218076B1 (en)

Also Published As

Publication number Publication date
PL218076B1 (en) 2014-10-31

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