PE20020784A1 - METHOD FOR IDENTIFYING TISSUE-SPECIFIC PROGESTERONE RECEPTOR BINDERS FOR ISOFORMS AND TISSUE SELECTIVE PROGESTERONE RECEPTOR BINDERS - Google Patents
METHOD FOR IDENTIFYING TISSUE-SPECIFIC PROGESTERONE RECEPTOR BINDERS FOR ISOFORMS AND TISSUE SELECTIVE PROGESTERONE RECEPTOR BINDERSInfo
- Publication number
- PE20020784A1 PE20020784A1 PE2001001311A PE2001001311A PE20020784A1 PE 20020784 A1 PE20020784 A1 PE 20020784A1 PE 2001001311 A PE2001001311 A PE 2001001311A PE 2001001311 A PE2001001311 A PE 2001001311A PE 20020784 A1 PE20020784 A1 PE 20020784A1
- Authority
- PE
- Peru
- Prior art keywords
- cells
- tissue
- gene
- plasmid
- progesterone receptor
- Prior art date
Links
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P5/00—Drugs for disorders of the endocrine system
- A61P5/24—Drugs for disorders of the endocrine system of the sex hormones
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P5/00—Drugs for disorders of the endocrine system
- A61P5/24—Drugs for disorders of the endocrine system of the sex hormones
- A61P5/30—Oestrogens
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5011—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing antineoplastic activity
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5082—Supracellular entities, e.g. tissue, organisms
- G01N33/5088—Supracellular entities, e.g. tissue, organisms of vertebrates
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
- G01N33/743—Steroid hormones
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
- G01N2333/72—Assays involving receptors, cell surface antigens or cell surface determinants for hormones
- G01N2333/723—Steroid/thyroid hormone superfamily, e.g. GR, EcR, androgen receptor, oestrogen receptor
Abstract
SE REFIERE A UN METODO PARA IDENTIFICAR LIGANDOS ESPECIFICOS DE LA ISOFORMA DEL RECEPTOR DE PROGESTERONA A O B (PR) QUE COMPRENDE: a)TRANSFECTAR EN FORMA ESTABLE UN GRUPO 1 DE CELULAS COMO CELULAS DEL NEUROBLASTOMA HUMANO CON UN PLASMIDO TAL COMO PROMOTOR DEL VIRUS DE TUMOR MAMARIO DEL RATON QUE EXPRESA LA ISOFORMA DEL RECEPTOR DE PR A; b)TRANSFECTAR EN FORMA ESTABLE UN GRUPO 2 DE CELULAS CON UN PLASMIDO QUE EXPRESA LA ISOFORMA DEL RECEPTOR DE PR B; c)TRANSFECTAR LOS GRUPOS 1 Y 2 DE CELULAS CON UN PLASMIDO QUE COMPRENDE UN GEN INFORMANTE TAL COMO EL GEN DE LUCIFERASA, CONECTADO CON UN PROMOTOR QUE RESPONDE A HORMONAS; d)PONER EN CONTACTO LOS GRUPOS 1 Y 2 DE CELULAS CON UN LIGANDO A EVALUAR; e)DETERMINAR LA EFICACIA DE LA TRANSCRIPCION O POTENCIA DEL GEN INFORMANTE EN LOS GRUPOS 1 Y 2 DE CELULAS; f)SELECCIONAR UN LIGANDO QUE TIENE UNA SELECTIVIDAD POR LA ISOFORMA DEL RECEPTOR DE PR A O B; LA DIFERENCIA EN LA EFICACIA DE LA TRANSCRIPCION ES MAYOR O IGUAL A 10% Y LA DIFERENCIA DE LA POTENCIA ES DE 10, QUE SE DETERMINA MIDIENDO LA ACTIVIDAD DE LA LUCIFERASA; LOS PLASMIDOS Y EL GEN COMPRENDEN UN ADEMAS UN GEN DE RESISTENCIA A ANTIBIOTICOS TAL COMO NEOMICINA O PUROMICINA. TAMBIEN SE REFIERE A UN METODO PARA IDENTIFICAR LIGANDOS DE RECEPTORES DE PR SELECTIVOS PARA TEJIDOS QUE COMPRENDE ADEMAS g)SOMETER EL LIGANDO SELECCIONADO A PRUEBAS IN VIVO EN UN PRIMER TEJIDO (DE MAMA) Y EN UN SEGUNDO TEJIDO (DE UTERO) BLANCO, h)SELECCIONAR EL LIGANDO QUE TIENE LA ACTIVIDAD DESEADAIT REFERS TO A METHOD FOR IDENTIFYING SPECIFIC LIGANDS OF THE PROGESTERONE AOB (PR) RECEPTOR ISOFORM, INCLUDING: a) TRANSFECTING IN A STABLE FORM A GROUP 1 OF CELLS AS HUMAN NEUROBLASTOMA CELLS WITH A PLASMID THUMAN PLASMID SUCH AS PRAMIRUS VAMUS OF THE MOUSE THAT EXPRESSES THE ISOFORM OF THE PR A RECEIVER; b) TRANSFECTING IN A STABLE FORM A GROUP 2 OF CELLS WITH A PLASMID THAT EXPRESSES THE ISOFORM OF THE PR B RECEIVER; c) TRANSFECTING GROUPS 1 AND 2 OF CELLS WITH A PLASMID THAT INCLUDES AN INFORMANT GENE SUCH AS THE LUCIFERASE GENE, CONNECTED WITH A PROMOTER THAT RESPONDS TO HORMONES; d) PUT IN CONTACT GROUPS 1 AND 2 OF CELLS WITH A LINKAGE TO BE EVALUATED; e) DETERMINE THE EFFECTIVENESS OF THE TRANSCRIPTION OR POWER OF THE REPORTING GENE IN GROUPS 1 AND 2 OF CELLS; f) SELECT A LIGAND THAT HAS A SELECTIVITY BY THE ISOFORM OF THE RECEIVER OF PR A OR B; THE DIFFERENCE IN THE EFFICIENCY OF THE TRANSCRIPTION IS GREATER OR EQUAL TO 10% AND THE DIFFERENCE IN THE POWER IS 10, WHICH IS DETERMINED BY MEASURING THE ACTIVITY OF THE LUCIFERASA; THE PLASMIDS AND THE GENE ALSO INCLUDE A GENE OF RESISTANCE TO ANTIBIOTICS SUCH AS NEOMYCIN OR PUROMYCIN. IT ALSO REFERS TO A METHOD FOR IDENTIFYING LIGANDS OF SELECTIVE PR RECEPTORS FOR TISSUES THAT ALSO INCLUDE g) SUBMITTING THE SELECTED LIGAND TO IN VIVO TESTING IN A FIRST TISSUE (OF BREAST) AND IN A SECOND TISSUE (OF UTERUS) SELECT THE LIGAND THAT HAS THE DESIRED ACTIVITY
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US25831200P | 2000-12-28 | 2000-12-28 | |
US30587501P | 2001-07-18 | 2001-07-18 |
Publications (1)
Publication Number | Publication Date |
---|---|
PE20020784A1 true PE20020784A1 (en) | 2002-10-03 |
Family
ID=26946565
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PE2001001311A PE20020784A1 (en) | 2000-12-28 | 2001-12-28 | METHOD FOR IDENTIFYING TISSUE-SPECIFIC PROGESTERONE RECEPTOR BINDERS FOR ISOFORMS AND TISSUE SELECTIVE PROGESTERONE RECEPTOR BINDERS |
Country Status (8)
Country | Link |
---|---|
EP (1) | EP1373888A2 (en) |
JP (1) | JP2004516841A (en) |
AR (1) | AR032050A1 (en) |
AU (1) | AU2002219218A1 (en) |
NO (1) | NO20032969L (en) |
PE (1) | PE20020784A1 (en) |
UY (1) | UY27107A1 (en) |
WO (1) | WO2002054064A2 (en) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1652768A (en) * | 2002-03-11 | 2005-08-10 | 舍林股份公司 | 5-(2-hydroxy-3-'1-(3-trifluoromethylphenyl)-cyclopropl-propionylamino)-phtalide and related compounds with progesterone receptor modulating activity for use in fertility control and hormone replaceme |
DE102007023614A1 (en) | 2007-05-21 | 2008-11-27 | Bayer Schering Pharma Aktiengesellschaft | New amide compounds used for treatment of gynecological diseases e.g. endometriosis, for female fertility control and female hormone replacement therapy, has general formula |
DE102007049630A1 (en) | 2007-10-11 | 2009-10-29 | Bayer Schering Pharma Aktiengesellschaft | New amide compounds used for therapy and/or prophylaxis of gynecological diseases e.g. endometriosis, for female fertility control and female hormone replacement therapy |
DE102007032800A1 (en) | 2007-07-10 | 2009-01-15 | Bayer Schering Pharma Aktiengesellschaft | Nonsteroidal progesterone receptor modulators |
DE102007058747A1 (en) | 2007-12-05 | 2009-06-10 | Bayer Schering Pharma Aktiengesellschaft | Nonsteroidal progesterone receptor modulators |
EP2070909A1 (en) | 2007-12-15 | 2009-06-17 | Bayer Schering Pharma AG | Non-steroidal progesterone receptor modulators |
CN113801889B (en) * | 2021-09-18 | 2023-04-07 | 中国农业科学院农业质量标准与检测技术研究所 | Cell screening model, construction method and application thereof, saccharomycete, preparation method and application thereof |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB8617653D0 (en) * | 1986-07-18 | 1986-08-28 | Ici Plc | Amide derivatives |
DE4235220A1 (en) * | 1992-10-13 | 1994-06-16 | Schering Ag | Gestagen effective 19,11ß-bridged 4-estrene |
DE4337416A1 (en) * | 1993-10-27 | 1995-05-04 | Schering Ag | 10,11beta-C¶2¶-bridged steroids |
US5506102A (en) * | 1993-10-28 | 1996-04-09 | Ligand Pharmaceuticals Incorporated | Methods of using the A form of the progesterone receptor to screen for antagonists of steroid intracellar receptor-mediated transcription |
DE4413185A1 (en) * | 1994-04-12 | 1995-10-19 | Schering Ag | 11beta, 19-bridged 13alpha alkyl steroids |
DE19723722A1 (en) * | 1997-05-30 | 1998-12-10 | Schering Ag | Nonsteroidal progestogens |
-
2001
- 2001-12-21 JP JP2002554712A patent/JP2004516841A/en active Pending
- 2001-12-21 AU AU2002219218A patent/AU2002219218A1/en not_active Abandoned
- 2001-12-21 WO PCT/EP2001/015200 patent/WO2002054064A2/en active Application Filing
- 2001-12-21 EP EP01272662A patent/EP1373888A2/en not_active Ceased
- 2001-12-26 AR ARP010106030A patent/AR032050A1/en not_active Application Discontinuation
- 2001-12-28 PE PE2001001311A patent/PE20020784A1/en not_active Application Discontinuation
- 2001-12-28 UY UY27107A patent/UY27107A1/en not_active Application Discontinuation
-
2003
- 2003-06-27 NO NO20032969A patent/NO20032969L/en not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
JP2004516841A (en) | 2004-06-10 |
NO20032969D0 (en) | 2003-06-27 |
WO2002054064A2 (en) | 2002-07-11 |
EP1373888A2 (en) | 2004-01-02 |
AR032050A1 (en) | 2003-10-22 |
AU2002219218A1 (en) | 2002-07-16 |
NO20032969L (en) | 2003-08-28 |
UY27107A1 (en) | 2002-03-22 |
WO2002054064A3 (en) | 2003-10-16 |
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FD | Application declared void or lapsed |