OA20250A

OA20250A - Composition comprising tolfenpyrad and pyraclostrobin.

Info

Publication number: OA20250A
Application number: OA1202100343
Authority: OA
Inventors: Pushpender Singh CHOUHAN; Dr. Shailendra Mitharam BHARAMBE; Dr. Ashim Kumar DUTTA; Ashutosh ACHARYA; Vishwanath GADE; Santosh Shridhar AUTKAR; Ravikumar Suryanarayana SARAGUR; Ruchi GARG
Original assignee: Pi Industries Limited
Filing date: 2020-01-15
Publication date: 2022-03-18

Abstract

The present invention discloses a synergistic combination of tolfenpyrad and pyraclostrobin with its composition and a method for controlling a wide variety of undesired pathogenic microorganisms, insect pests and mites, for a plant, including the treatment of plant/plant parts and a region around the plant.The present invention also discloses an enhanced efficacy against pests and diseases in comparison to individual components of the said combination. The combination also widens the spectrum and said to have longer residual effects against undesired pathogenic microorganisms, insect pests and mites.

Description

COMPOSITION COMPRISING TOLFENPYRAD AND PYRACLOSTROBIN

FIELD OF THE INVENTION:

The présent invention relates to a combination of tolfenpyrad and pyraclostrobin and its composition and methods of préparation thereof. Particularly, the présent invention relates to asynergistic composition comprising tolfenpyrad and pyraclostrobin, methods of préparation of thecomposition and use thereof for the control of a wide variety of undesired phytopathogenic microorganisms, insect pests and mites.

BACKGROUND OF THE INVENTION:

In many economically important crops, such as rice, peppers, tomatoes, grapes, peas, cotton and other agricultural and horticultural crops, fungal infection and insect infestation may occur simultaneously for which growers are applying control measures either separately or by simply tank mixing various insecticides and fungicides together. This practice of mixing various pesticides might lead to poor efficacy and crop damage due to compatibility issues hence a premix of the best possible formulation for a combination of an insecticide and a fungicide would help growers to combat these important diseases and insects/mites in those crop stages.

Typical problems arising in the field of pest control is to reduce the dose rates of the active ingrédients in order to reduce or avoid unfavorable environmental or toxicological effects whilst still allowing effective disease/pest control. Therefore, a combined application of an effective amount of fungicide and insecticide as a uniform composition is a practical necessity.

It is known in the art that certain pyrazole carboxamide dérivatives hâve insecticidal as well as fungicidal propertiesfor example tolfenpyrad.Tolfenpyrad is a contact insecticide and fungicide,which was developed by Mitsubishi Chemical (now Nihon Nohyaku), as described in US 5,039,693. It is used to control or suppress a wide range of insect pests and diseases including Lepidoptera, Coleoptera, Diptera, Hemiptera, Orthoptera, and Thysanoptera insects, and downy mildew and powdery mildew.lt is effective in controlling ail developmental stages of most target pests. It is classified under group 21A of the IRAC mode of action andgroup 39 of FRAC mode of action.Tolfenpyrad is a MET1 inhibitor, which inhibits cell respiration when it cornes in contact with the target pests.

Chemically, it is 4-chloro-3-ethyl-l-methyl-W-[4-(p-tolyloxy)benzyl]-lH-pyrazole-5carboxamide and has the following structure:

Strobilurin dérivatives hâve fungicidal activity, for example, pyraclostrobin, azoxystrobin, fluoxastrobin, trifloxystrobin,pyribencarb, or pyraoxystrobin. Pyraclostrobin is a broad spectrumfoliar fungicide, developed by BASF, as described in US 5,869,517. It is classifïed under group 11 of the FRAC mode of action, which blocks the mitochondrial électron transport and thus inhibits the energy supply of the fungus which results in the death of the target fungus. Currently, it is used to controlvarious fungal diseasessuch asdowny mildew, powdery mildew,black shank, black spot, melanose, post bloom fruit drop, anthracnose, alternaria brown spot, scab or greasy spot,leaf rust, black point, net blotch, scald, septoria leafandglume blotch, spot blotch, stem rust, stripe rust,yellow leaf spot,eyespot, gray leaf spot, northern corn leaf spot, physoderma brown spot, rust, Southern corn leaf blight or yellow leaf blight.

Chemically, it is methyl 2-[l-(4-chlorophenyl)-lH-pyrazol-3-yloxymethyl]-Nmethoxycarbanilate and has the following structure:

Various patent applications disclose the mixtures of fungicides and insecticides for e.g. US20120322654, US20080261811 and WO2011144593.There is no such effective composition available that act simultaneously on a wide variety of fungi and pest.

It has been found that, as a solution to the above mentioned problems, a combination comprising a pyrazolecarboxamide class compound and a strobilurin class compound provides an effective composition in controlling a wide variety of undesired phytopathogenic microorganisms, insect pests and mites. More, specifically,the combinationcomprisestolfenpyrad and pyraclostrobin.

The components of the combination of the présent invention hâve a similar site of action. Tolfenpyrad acts by inhibiting complex-I in the mitochondrial electron-transport chain of mitochondria and pyraclostrobin acts by inhibiting the complex-ΠΙ in mitochondrial respiration. The présent invention provides a combination of tolfenpyrad and pyraclostrobin with enhanced efficacy and spectrum as compared to the use of tolfenpyrad and pyraclostrobin alone.

SUMMARY OF THE INVENTION:

In one aspect, the présent invention provides a composition comprising of:

a) an effective amount of tolfenpyrad or salt(s) thereof,

b) an effective amount of pyraclostrobin or salt(s) thereof,

c) optionally, an addition of one or more insecticidal or fungicidal or acaricidal or nematicidal or herbicidal or plant growth regulator compounds or any combination thereof,

d) agriculturally acceptable additives.

In a particular aspect, the présent invention provides a synergistic combination of tolfenpyrad and pyraclostrobin to control a wide variety of undesired phytopathogenic microorganisms, insect pests and mites.

In another aspect, the présent invention provides a synergistic fungicidal and insecticidal combination of tolfenpyrad and pyraclostrobin.

In yet another aspect, the présent invention provides a synergistic fungicidal combination of tolfenpyrad and pyraclostrobin.

In a further aspect, the présent invention provides a synergisticinsecticidal combination of tolfenpyrad and pyraclostrobin.

In another aspect, the présent invention is to provide longer residual control of the undesired phytopathogenic microorganisms, insect pests and mites treated with the combination of tolfenpyrad and pyraclostrobin.

In a further aspect, the présent invention provides asynergistic composition comprising of:

a) an effective amount of tolfenpyrad or salt(s) thereof,

b) an effective amount of pyraclostrobin or salt(s) thereof,

d) agriculturally acceptable additives.

In one aspect, the présent invention provides a synergistic composition of tolfenpyrad and pyraclostrobin to control a wide variety of undesired phytopathogenic microorganisms, insect pests and mites.

In a furtheraspect, the présent invention provides a synergistic composition comprising tolfenpyrad and pyraclostrobin; thecomposition possesses fungicidal and/or insecticidal activity.

In another aspect, the présent invention provides a method for the préparation of the composition comprising of tolfenpyrad and pyraclostrobin.

In a further aspect, the présent invention provides a method for improving crop health (phytotonic effect), comprising treating a plant with an effective amount of the composition of tolfenpyrad and pyraclostrobin.

The above aspectsand other objectives will become more apparent in view of the description given below.

DETAILED DESCRIPTION OFTHE INVENTION:

ABBREVIATIONS

AE: Aérosol	ME: Micro-emulsion
CS: Capsule suspension	OD: Oil dispersion
DC: Dispersible concentrate	SC: Suspension concentrate
DP: Dustable powder	SU: Ultra-low volume (ULV) suspension
EC: Emulsifiable concentrate	UL: Ultra-low volume (ULV) liquid
EG: Emulsifiable granule	WDG: Water dispersible granule
e.g.: Example	WP: Wettable powder
EP: Emulsifiable powder	WT: Water dispersible tablet
EW: Oil in water émulsion

DEFINITIONS

The foregoing définitions provided herein for the terminologies used in the présent disclosure are for illustrative purposes only and in no manner limit, the scope of the présent invention disclosed in the présent disclosure.

It will be understood that the terminology used herein is for the purpose of describing embodiments only, and is not intended to be limiting. As used in this spécification, the singular forms “a”, “an” and “the” include plural referents unless the context clearly dictâtes otherwise. Thus, for example, the reference to “a surfactant” includes one or more such surfactants.

Unless defined otherwise, ail technical and scientific ternis used herein hâve the same meaning as commonly understood by one of ordinary skill in the art to which the invention pertains. Although other methods and materials similar, or équivalent, to those described herein can be used in the practice of the présent invention, the preferred materials and methods are described herein.

As used herein, the terms “comprises”, “comprising”, “includes”, “including”, or any other variation thereof, are intended to cover a non-exclusive inclusion, subject to any limitation explicitly indicated. For example, a composition or a method that comprises a list of éléments is not necessarily limited to only those éléments but may include other éléments not expressly listed or inhérent to such composition, or method.

As used herein, the term “composition” or “formulation” can be used interchangeably, unless stated otherwise, is meant to encompass, and are not limited to, compositions or formulations containing the combination of tolfenpyrad and pyraclostrobin.

As used herein, the term “insecticidal” refers to the ability of a composition or substance to improve the ability of insect mortality or inhibit the growth rate.

As used herein the terni “fungicidal” refers to the ability of a composition or substance to reduce the rate of fungal growth or increase fungal mortality.

As used herein the terni “neniaticidal” refers to the ability of a composition or substance to improve the ability of the nematode mortality or inhibit the growth rate.

As used herein the terni “acaricidal” refers to the ability of a composition or substance belonging to the Arachnida increase mortality ectoparasites Acari or its ability to inhibit the growth rate.

As used herein the terni “herbicidal”, refers to the ability of a composition or substance that is applied to an area of interest (eg, a field or area of cultivation) before the plants appear visibly from the soil and / or before germination of the seeds or that is applied to an area after a plant has visibly emerged from the soil.

As used herein the term “plant growth regulator” refers to the ability of a composition or substancethat serves to modify the growth and development of a treated plant to an agricultural maturity state without killing the treated plant.

As used herein, the term “additive(s)” or “auxiliary agent(s)” or “agriculturally acceptable canier(s)”can be used interchangeably and refers to inert substances which are commonly used as diluent, to piovide stability or to increase the activity profile of the composition or formulation with or without having agrochemical activity or direct effect on the undesired pathogenic microorganism, insect pests and mites.

As used herein, the term “surfactant(s)” means a compound that, when dissolved in a liquid, reduces the surface tension of the liquid, which reduces the interfacial tension between two liquids or which reduces surface tension between a liquid and a solid.

As used herein, the term “stabilizer(s)” refers to a substance capable of imparting résistance against physical or Chemical détérioration or deformulation.

As used herein, the term “dcfoamer(s)” refers to a Chemical additive that reduces and hinders the formation of foam in the industrial process of liquids, semi-solids, or solids. The terms defoamer, defoaming agent and anti-foaming agent can be used interchangeably.

As used herein, the term “thickener(s)” refers to a polymeric material, which at a low concentration increases the viscosity of an aqueous solution and helps to stabilize the composition.

Unless otherwise specified, % refers to % weight; and % weight refers to % of the weight of respective component with respect to the total weight of the composition.

As used herein, the term “locus” means a plant, plant parts, plant propagation material (preferably seed), soil, area, material or environment in which a pest is growing or may grow. As used herein, the term “plant parts” are understood to mean ail above-ground and belowground parts and organs of plants, such as shoot, leaf, flower and root, examples including leaves, needles, stems, stalks, flowers, fruit-bodies, fruits and seeds, and also roots, tubers and rhizomes. The plant parts also include harvested plants and végétative and generative propagation material, for example seedlings, tubers, rhizomes, cuttings and seeds.

As used herein, the term “effective amount” means the amount of the active substances in the compositions to achieve an observable effect on growth, including the effects of necrosis, death, retardation, prévention, and removal, destruction, or otherwise diminishing the occurrence and activity ofthe target organism. The effective amount can vary for the various compositions used in the présent invention. An effective amount of the compositions will also vary according to the prevailing conditions such as desired pesticidal effect and duration, weather conditions, target species, locus, mode of application, and the like.

As used herein, the term “tolfenpyrad”encompasses tolfenpyrad or its agrochemical acceptable salt(s), ester(s), derivative(s) or any other modified form of tolfenpyrad.

As used herein, the term “pyraclostrobin” encompasses pyraclostrobin or its agrochemical acceptable salt(s), ester(s), derivative(s) or any other modified form of pyraclostrobin.

In one embodiment, the présent invention provides a composition comprising of:

a) an effective amount of tolfenpyrad or salt(s) thereof,

b) an effective amount of pyraclostrobin or salt(s) thereof,

d) agriculturally acceptable additives.

In another embodiemnt, the présent invention provides a synergistic composition comprising of:

a) an effective amount of tolfenpyrad or salt(s) thereof,

b) an effective amount of pyraclostrobin or salt(s) thereof,

d) agriculturally acceptable additives.

In one embodiment, the présent invention provides a synergistic combination of tolfenpyrad and pyraclostrobin to control a wide variety of undesired phytopathogenic microorganisms, insect pests and mites.

In a further embodiment, the présent invention provides a fungicidal and insecticidal combination of tolfenpyrad and pyraclostrobin.

In yet another embodiment, the présent invention provides a fungicidal combination of tolfenpyrad and pyraclostrobin.

In a further embodiment, the présent invention provides aninsecticidal combination of tolfenpyrad and pyraclostrobin.

In another embodiment, the présent invention provides a longer residual control of the undesired phytopathogenic microorganisms, insect pests and mites treated with the combination of tolfenpyrad and pyraclostrobin.

The combination ratio of tolfenpyrad and pyraclostrobin dépends on various factors such as, the undesired phytopathogenic microorganisms, insect pests and mites to be controlled, the degree of infestation, the climatic conditions, the characteristics of the soil and the application method, wherein the ratio of tolfenpyrad and pyraclostrobin is 50:1 to 1:50, preferably 20:1 to 1: 20 or 10: 1 to 1: 10, more preferably 3:2 to 2:3.

In one embodiment, the présent invention further provides a composition of tolfenpyrad and pyraclostrobin for the control of a wide variety of undesired phytopathogenic microorganisms, insect pests and mites.

In another embodiment, the présent invention provides a composition of tolfenpyrad and pyraclostrobin, wherein the composition is used as fungicidal and/or insecticidal composition. The composition is selected from wettable powder (WP), a water dispersible granule (WDG), a water dispersible tablet (WT), a suspension concentrate (SC), an ultra-low volume (ULV) liquid (UL), an ultra-low volume (ULV) suspension (SU), an emulsifiable granule (EG), an oil-in-water émulsion (EW), an emulsifiable concentrate (EC), an emulsifiable powder (EP), a dispersible concentrate (DC), a micro-emulsion (ME), an oil dispersion (OD), a capsule suspension (CS), a dustable powder (DP) or an aérosol (AE).

In a preferred embodiment, the présent invention provides a water dispersible granule composition of tolfenpyrad and pyraclostrobin.

In another preferred embodiment, the présent invention provides a suspension concentrate composition of tolfenpyrad and pyraclostrobin.

These compositions may be manufactured by any process known in the art, such as,“Pesticide

Formulation Guide” (edited by Pesticide Science Society of Japan, The Agricultural

Formulation and Application Committee, published by Japan Plant Protection Association,

1997).

The composition of the present invention comprises of tolfenpyrad and pyraclostrobin in a wider ratio, in relation to various factors such as, the undesired phytopathogenic microorganisms, insect pests and mites to be controlled, the degree of infestation, the climatic conditions, the characteristics of the soil and the application method, wherein the weight ratio of tolfenpyrad and pyraclostrobin is 50:1 to 1:50, preferably 20:1 to 1: 20 or 10: 1 to 1: 10, more preferably 3:2 to 2:3.

Accordingly, the present invention further provides a composition for the control of a wide variety of undesired phytopathogenic microorganisms, insect pests and mites, wherein the weight percentage of tolfenpyrad is about 4% to 80%, preferably about 10% to 60%and the weight percentage of pyraclostrobin is 80% to 4%, preferably about 60% to 10% and the weight percentage of additives is about 5% to 95%, preferably about 20% to 90%. The total content of tolfenpyrad and pyraclostrobin in the composition is selected in the weight range typically from 8% to 84%, preferably from 10% to 80%, more preferably from 12% to 75%.

The composition comprising organic or inorganic carrier materiai including agriculturally acceptable additive(s) is selected from the group comprising of solid carrier(s), liquid carrier(s), gaseous camer(s), surfactant(s), binder(s), disintegrating agent(s), pH adjuster(s), thickener(s), preservative(s), anti-freezing agent(s), defoaming agent(s), extender(s), stabilizer(s) and/or coloring agent(s) or a combination thereof.The composition may also contain if desired, one or more auxiliaries customary for crop protection compositions.

Solid carrier(s) is selected from the group comprising of, but not limited to, natural minerais quartz, talc, kaolin, pyrophyllite, montmorillonite, attapulgite, bentonite, chalk, zeolite, calcite, sericite, acid clay, diatomaceous earth, Fuller’s earth, meerschaum, gibbsite, dolomite or pumice; synthetic minerais such as precipitated silica, fumed silica, sodium silicate, alumina, aluminum hydroxide; inorganic salts such as calcium carbonate, ammonium sulfate, sodium sulfate, potassium chloride, organic materials such as urea, solid polyoxyethylene, solid polyoxypropylene, polyethylene, polypropylene, lactose, starch, lignin, cellulose, cottonseeds hulls, wheat flour, soyabean flour, wood flour, walnut shell flour, plant powders, sawdust, coconut shellflower, corn cob, tobacco stem. These solid carriers may be used alone or in combination.

Liquid carrier(s) is selected from the group comprising of, but not limited to, water; alcohols such as éthanol, propanol, butanol, n-octanol, isopropanol ethylene glycol, diethylene glycol, propylene glycol, polyethylene glycol, benzyl alcohol, glycerin; polyol ethers such as ethylene glycol monopropyl ether, diethylene glycol monomethyl ether, dipropylene glycol dimethyl ether; ketones such as acetone, methyl ethyl ketone, methyl isobutyl ketone, cyclohexanone; ethers such as dipropyl ether, dioxane, tetrahydrofuran, aliphatic hydrocarbons such as normal paraffin, isoparaffin, kerosene, minerai oil; aromatic hydrocarbons such as xylene, toluene, naphthene, solvent naphtha, solvent C9, solvent Cio, solvent C12, solvesso 100, solvesso 150, solvesso 200; chlorinated aliphatic or aromatics hydrocarbons such as chlorobenzene, chloroethylene, methylene chloride; esters such as ethyl acetate, diisopropyl phthalate, dimethyl adipate, methyl oleate, methyl tallowate; lactones such as gamma-butyrolactone, gamma-valerolactone, epsilon-caprolactone; amides such as dimethylformamide, N-methylpyrrolidone, N-octylpyrolidone, N,N-dimethyldecanamide; nitriles such as acetonitrile; organosulfur compound such as dimethyl sulfoxide; vegetable oils such as soybean oil, rapeseed oil, cotton seed oil. These liquid carriers may be used alone or in combination.

Gaseous carrier(s) is selected from the group comprising of, but not limited to, liquefied Petroleum gas, air, nitrogen, carbon dioxide or dimethyl ether. These gaseous carriers may be used alone or in combination thereof.

Surfactant(s) (a dispersing agent, a wetting agent, a spreader, an adjuvant for pénétration enhancement, rain fastness, soil leaching control etc.) are nonionic or anionic surfactants or a combination of these surfactants. It is preferred to use one or more than one kind of surfactant. Surfactant(s) is selected from the group comprising of, but not limited to, sugar esters such as sorbitan monolaurate, polyoxyethylene sorbitan monolaurate; alkyl polyglucoside such as decyl glucoside; polyoxyethylene alkyl ethers such as polyoxyethylene lauryl ether, or polyoxyethylene coconut fatty alcohol ether; polyoxyethylene alkynyl ether such as polyoxyethylene 2,4,7,9-tetramethyl-5-decyn-4,7-diol ether; polyoxyethylene aryl ethers such as polyoxyethylene nonylphenyl ether or polyoxyethylene tristyrylphenyl ether;

polyoxyethylene vegetable oil ethers such as polyoxyethylene castor oil or polyoxyethylene hydrogenated castor oil; vegetable oil ethoxylate; alcohol C12-C15 ethoxylate; polyoxyethylene fatty acid esters such as polyoxyethylene monolaurate, polyoxyethylene distearate or polyoxyethylene resin acid ester; polyoxyethylene polyoxypropylene (EO-PO) block co-polymers such as Pluronic®; polyoxyethylene polyoxypropylene alkyl ether such as polyoxyethylene polyoxypropylene lauryl ether; polyoxyethylene polyoxypropylene aryl ether such as polyoxyethylene polyoxypropylene styrylphenyl ether; polyoxyethylene alkyl amines such as polyoxyethylene stearyl amine; polyoxyethylene fatty acid amide such as lauric acid diethanolamid; fluorinated surfactant; alkyl sulfates such as sodium lauryl sulfate; sodium alkylbenzene sulfonate, calcium alkylbenzene sulphonate; polyoxyethylene alkyl ether sulfates such as sodium polyoxyethylene lauryl ether sulfate; polyoxyethylene aryl ether sulfates such as sodium polyoxyethylene nonylphenyl ether sulfate or ammonium polyoxyethylene tristyrylphenyl ether sulfate; aryl sulfonate such as calcium dodecyl benzene sulfonate, sodium naphthalene sulfonate or sodium naphthalene sulfonate formaldéhyde condensate; poly aryl phenyl ether sulphate ammonium sait; ‘alpha’-olefin sulfonate; alkyl sulfosuccinate such as sodium dioctyl sulfosuccinate; sodium lignosulfonate; polycarboxylic acid sodium sait; N-methyl fatty acid sarcosinate; polyoxyethylene alkyl ether phosphate; polyoxyethylene aryl ether phosphates such as polyoxyethylene phenyl ether phosphate; polyoxyethylene alkyl phenyl ether phosphate; graft co-polymers such as polymethyl methacrylate-polyethylene glycol graft copolymer; or a combination thereof.

Binder(s) or adhesive-imparting agent(s) is selected from the group comprising of, but not limited to,polyvinyl alcohol, dextrin, denatured dextrin, soluble starch, guar gum, xanthan gum, sucrose, polyvinylpyrrolidone, gum arabic, polyvinyl acetate, sodium polyacrylate, carboxymethyl cellulose or its sait, carboxymethylcellulose dextrin, bentonite, polyethylene glycol having an average molecular weight of 6,000 to 20,000, polyethylene oxide having average molecular weight of 100,000 to 5,000,000, natural phosphatide such as cephalinic acid or lecithin. These binders or adhesive-imparting agents may be used alone or in combination thereof.

Disintegrating agent(s)is selected from the group comprising of, but not limited to, sodium tripolyphosphate, stearic acid métal sait, cellulose powder, dextrin, méthacrylate co-polymer, polyvinylpyrrolidone, polyaminocarboxylic acid chelate compound, styrene sulfonate/isobutylene/maleic anhydride co-polymer, starch/polyacrylonitrile graft co12 polymer, sodium hexametaphosphate, carboxymethyl cellulose, sodium polycarbonate, bentonite. These disintegrating agents may be used alone or in combination thereof.

pH adjuster(s)is selected from the group comprising of, but not limited to, sodium or potassium carbonate, sodium or potassium hydrogen carbonate, sodium or potassium dihydrogenphosphate, disodium or dipotassium hydrogenphosphate, citric acid, malic acid and triethanolamine. These pH adjusters may be used alone or in combination thereof.

Thickener(s)is selected from the group comprising of, but not limited to, water-soluble polymer and inorganic fine powder, wherein the examples for water-soluble polymers are xanthan gum, welan gum, guar gum, polyvinyl alcohol, carboxymethylcellulose, polyvinylpyrrolidone, carboxyvinyl polymer, acrylic polymer, acrylic graft copolymer, starch dérivative or polysaccharide; or an inorganic fine powder selected from high purity silica, bentonite, white carbon. These thickeners may be used alone or in combination thereof.

Preservative(s)is selected from the group comprising of, but not limited to, potassium sorbate, 4-hydroxybenzoic acid esters, 2-methyl-4-isothiazolin-3-one, 5-chloro-2-methyl-4isothiazolin-3-one, l,2-benzisothiazolin-3-one (Proxel® GXL). These preservatives may be used alone or in combination thereof.

Anti-freezing agent(s)is selected from the group comprising of, but not limited to, polyhydric alcohol such as ethylene glycol, diethylene glycol, propylene glycol, polyethylene glycol and glycerol. These anti-freezing agents may be used alone or in combination thereof.

Defoamer(s)is selected from the group comprising of, but not limited to, silicone compounds such as polysiloxane, polydimethylsiloxane and organic fluorine compounds. These defoamers may be used alone or in combination thereof.

Extender(s)is selected from the group comprising of, but not limited to, Silicon type surfactant, a cellulose powder, dextrin, processed starch, a polyaminocarboxylic acid chelate compound, crosslinked polyvinylpyrrolidone, maleic acid and styrene, methacrylic acid copolymer, half ester of polymer of polyhydric alcohol with dicarboxylic anhydride, watersoluble sait of polystyrène sulfonic acid. These extenders may be used alone or in combination thereof.

Stabilizer(s)is selected from the group comprising of, but not limited to, drying agents such as zeolite, quick lime or magnésium oxide; antioxidant agent such as phénol type, amine type, sulfur type or phosphorus type; or ultraviolet absorber such as salicylic acid type or a benzophenone type. These stabilizers may be used alone or in combination thereof.

Coloring agent(s)is selected from the group comprising of, but not limited toinorganic pigment such as iron oxide, titanium oxide or Prussian blue; organic dye such as alizarin dye, azo dye, métal phthalocyanine dye. These coloring agents may be used alone or in combination thereof.

When additive ingrédients are incorporated into the composition of the présent invention, the content of the carrier is selected typically in weight range of about 5% to 95%, preferably about 20% to 90%; the content of the surfactant is selected from the range of typically about 0.1% to 30%, preferably about 0.5% to 10%, and the content of the other additives is selected from the range of typically about 0.1% to 30%, preferably about 0.5% to 10%.

The composition of the présent invention can be applied by any one of the methods selected from atomization, spreading, dusting, spraying, diffusion, immersion, irrigation, injection, mixing, sprinkling (water immersion), foaming, dressing, coating, blasting, fumigation, smoking, smog and painting.

In an embodiment, the présent invention provides a method of controlling a wide variety of undesired phytopathogenic microorganisms, insect pests and mites, with an effective amount of a composition of tolfenpyrad and pyraclostrobin.

In an embodiment, the présent invention provides a method for controlling undesired phytopathogenic microorganisms with an effective amount of the composition of tolfenpyrad and pyraclostrobin.

The undesired pathogenic microorganism for the présent invention is selected from the group comprising of Albugo spp. (white rust) on omamentals, vegetables (e.g.A. Candida) and sunflowers (e.g.A. tragopogonis); Alternaria spp. (Altemaria leaf spot) on vegetables, citrus fruits (A. citri), râpe (A. brassicola or brassicae), sugar beets (A. tennis), fruits, rice, soybeans, potatoes (e.g.A. solani or A. alternata), tomatoes (e.g.Â. solani or A. alternata) and wheat; Aphanomyces spp. on sugar beets and vegetables; Ascochyta spp. on cotton, cereals and vegetables, e.g.A. tritici (anthracnose) on wheat and A. hordei on barley; Bipolaris and Drechslera spp. (teleomorph: Cochliobolus spp. for e.g. Cochliobolus carbonum (northem corn leaf blight)), e.g. Southern leaf blight (D. maydis) or Northem leaf blight (B. zeicola) on corn, e.g. spot blotch (B. sorokiniana) on cereals, e.g. B. oryzae on rice and turfs and on oats; Blumeria (formerly Erysiphé) graminis (powdery mildew) on cereals (e.g. on wheat or barley); Botrytis cinerea (teleomorph: Botryotinia fuckeliana: grey mold) on fruits and berries (e.g. strawbemes), vegetables (e.g. lettuce, carrots, celery and cabbages), râpe, flowers, vines, forestry plants and wheat; Bremia lactucae (downy mildew) on lettuce; Ceratocystis (syn. Ophiostoma) spp. (rot or wilt) on broadleaved trees and evergreens, e.g.C. ulmi (Dutch elm disease) on elms; Cercospora spp. (Cercospora leaf spots) on corn and cotton, (Cercospora blight spots) on cotton, (e.g. Gray leaf spot: C. zeae-maydis), rice, sugar beets (e.g.C. beticola), sugar cane, vegetables, coffee, soybeans (e.g.C. sojina or C. kikuchii) and rice, sunflower (e.g. cercospora leaf spot: C. helianthï), peanut (e.g. early leaf spot: C. arachidicola); Cercosporidium spp. on peanut (e.g. C. personatum: late leaf spot); Cladosporium spp. on tomatoes (e.g.C. fulvum: leaf mold) and cereals, e.g.C. herbarum (black car) on wheat, C. caiyigenum (pecan scab) on pecan; Cylindrocladium spp. on peanut (C. crotalariae: cylindrocladium black rot); Claviceps purpurea (ergot) on c&vvàW.Cochliobohis (anamorph: Helminthosporium of Bipolaris) spp. (leaf spots) on corn (C. carbonum), cereals (e.g.C. sativuslfiAack point), anamorph: B. sorokiniana) and rice (e.g.C. miyabeanus, anamorph: H. oryzae); Colletotrichum (teleomorph: Glomerella) spp. (anthracnose) on cotton (e.g.C. gossypii), com (e.g.C. graminicola: Anthracnose stalk rot), soft fruits, potatoes (e.g. C. coccodes: black dot), beans (e.g.C. Hndemuthianum), citrus fruits (e.g. C. acutatumlpost bloom fruit drop), C. gloeosporioides) and soybeans (e.g. C. truncatum or C. gloeosporioides); Corticium spp., e.g. C. sasakii (sheath blight) on rice; Corynespora cassiicola (leaf spots) on soybeans and omamentals; Cycloconium spp., e.g. C. oleaginum on olive trees; Cylindrocarpon spp. (e.g. fruit tree canker or young vine décliné, teleomorph: Nectria or Neonectria spp.) on fruit trees, vines (e.g.C. liriodendri, teleomorph: Neonectria liriodendri·. Black Foot Disease) and omamentals; Dematophora (teleomorph: Rosellinia) necatrix (root andstem rot) on soybeans; Diplodia spp. e.g. Diplodia boll rot on cotton, Diaporthe spp., e.g.D. phaseolorum (damping off) on soybeans, D. citri (melanose) on citrus fruits; Drechslera (syn. Helminthosporium, teleomorph: Pyrenophora) spp. on corn,cereals, such as barley (e.g.D. teres, net blotch), oats (e.g. D. avenae, leaf spot), and wheat (e.g.D. tritici-repentis: tan spot), rice and turf; Esca (dieback, apoplexy) on vines, caused by Formitiporia (syn. Phellinus) punctata, F. mediterranea, Phaeomoniella chlamydospora (earlier Phaeoacremonium chlamydosporum), Phaeoacremonium aleophilum and/or Botryosphaeriaobtusa; Elsinoe spp. on pome fruits (E. pyri), on citrus fruits (E. fawcetti), soit fruits (E. veneta'. anthracnose) and vines (E. ampelina: anthracnose); Entyloma oryzae (leaf smut) on rice; Epicoccum spp.(black mold) on wheat; Erysiphe spp. (powdery mildew) on sugar beets (E. betae),(powdery mildew) on rye (E. graminis), vegetables (e.g.P. pisi), such as cucurbits (e.g.P. cichoracearum), cabbages, sunflower, rape(e.g.P. cruciferarum), peas and bean (e.g. E. polygoni); Eutypa lata (Eutypa canker or dieback, anamorph: Cytosporinalata, syn. Libertella blepharis) on fruit trees, vines and omamental woods; Exserohilum(syn. Helminthosporiurri) spp. on corn (e.g.E. turcicum); Fusarium (teleomorph: Gibberella)sgg. (wilt, root or stem rot) on various plants, such as hardlock, boll rot of cotton, F. graminearum or F.culmorum (root rot, scab or head blight) on cereals (e.g. wheat or barley), F. oxysporumon tomatoes, F. solani on soybeans and F. verticillioides on corn; Gaeumannomycesgraminis (take-all) on cereals (e.g. wheat or barley) and corn; Gibberella spp. on cereals (e.g.G. zeae) and rice (e.g.G. fujikuroi: Bakanae disease); Glomerella cingulata on vines, pome fruits and other plants and G. gossypii on cotton; Grain staining complex on rice; Guignardia bidwellii (black rot) on vines, Guignardia citricarpa (balck spot) on citrus fruits; Gymnosporangium spp. on rosaceous plants and junipers, e.g.G. sabinae (rust) on pears; Helminthe sporium spp. (syn. Drechslera, teleomorph: Cochliobolus) on corn, cereals and rice; Hemileia spp., e.g.//. vastatrix (coffee leaf rust) on coffee; Isariopsis clavispora (syn. Cladosporium vitis) on vines; Kabatiella zeae (eyespot) on corn; Leptosphaeria macidans (blackleg) on oilseed crops; Leptosphaerulina spp. on peanut (e.g. L. crassiasca: pepperspot); Macrophomina phaseolina (syn. phaseoli) (root and stem rot) on soybeans and cotton; Microdochium (syn. Fusarium) nivale (pink snow mold) on cereals (e.g. wheat or barley); Microsphaera diffusa (powdery mildew) on soybeans; Myeosphaerella citri (greasy spot) on citrus fruit, Monilinia spp., e.g.M laxa, M. fructicola and M. fructigena (bloom and twig blight, brown rot) on stone fruits and other rosaceous plants; Myeosphaerella spp. on peas and beans, cereals, bananas, soft fruits and ground nuts, such as e.g.M graminicola (anamorph: Septoria tritici, Septoria blotch) on wheat or M. fijiensis (black Sigatoka disease) on 'oanana^Peronospora spp. (downy mildew) on cabbage (e.g.P. brassicae), râpe (e.g.P. parasitica), onions (e.g.P. destructor), tobacco (P. tabacina) and soybeans (e.g.P. manshurica); Phyllosticta maydis (yellow leaf blight) on corn; Phakopsora pachyrhizi and P. meibomiae (soybean rust) on soybeans; Phialophora spp. e.g. on vines (e.g.P. tracheiphila and P. tetraspora) and soybeans (e.g.P. gregata: stem rot); Phoma

Hngam(root and stem rot) on râpe and cabbage and P. betae (root rot, leaf spot and dampingoff) on sugar beets, Phoma exigua (ascochyta blight) on peas and beans, phoma blight, boll rot on cotton, Phoma arachidicola (web blotch) on peanut; Phomopsis spp. on sunflowers, vines (e.g.P. viticola: can and leaf spot) and soybeans (e.g. stem rot:, phaseoli, teleomorph: Diaporthe phaseolorum)·, Phykopsora spp. e.g. rust on cotton, Physoderma maydis (physoderma brown spot) on corn; Phytophthora spp. (wilt, root, leaf, fruit and stem root) on various plants, such as paprika and cucurbits (e.g.F. capsici), soybeans (e.g./’. megasperma, syn. P. sojae), potatoes and tomatoes (e.g.R. infestons: late blight), broad-leaved trees (e.g.P. ramorum: sudden oak death) and peas and beans (e.g. P. nicotianae: downy mildew); Plasmodiophora brassicae (club root) on cabbage, râpe, radish and other plants; Plasmopara spp., e.g.P. viticola (downy mildew) on vines and P. halstedii on sunflowers; Podosphaera spp. (powdery mildew) on rosaceous plants, hop, pome and soft fruits, e.g.P. leucotricha on apples; Polymyxa spp., e.g. on cereals, such as barley and wheat (P. graminis) and sugar beets (P. betae) and thereby transmitted viral diseases; Pseudocercosporella herpotrichoides (eyespot, teleomorph: Tapesia yallundae) on cereals, e.g. wheat or barley; Pseudoperonospora (downy mildew) on various plants, e.g.P. cubensis on cucurbits or P. humili on hop; Pseudopezicula tracheiphila (red fire disease or rotbrenner, anamorph: Phialophora) on vines; Puccinia spp. (rusts) on various plants, e.g.P. triticina (brown or leaf rust), P. striiformis (stripe or yellow rust), P. hordei (dwarf rust), P. graminis (stem or black rust) or P. recondita (brown or leaf rust) on cereals, such as e.g. wheat, barley or rye (P. recondita), P. kuehnii (orange rust) on sugar cane and P. asparagi on asparagus, P. coronata (crown rust) and P. graminis (stem rust) on oats, P. arachidis (rust) on peanut; Pyrenophora (anamorph: Drechslera) tritici-repentis (tan spot) on wheat,P. feres (net blotch) on barley, or P. avenae (leaf blotch) on oats; Pyricularia spp., e.g.P. oryzae (teleomorph: Magnaporthe grisea, rice blast) on rice and P. grisea on turf and cereals; Pythium spp. (damping-off) on turf, rice, corn, wheat, cotton, râpe, sunflowers, soybeans, sugar beets, vegetables and various other plants (e.g./’. ultimum or P. aphanidermatum)', Ramularia spp., e.g./?. collo-cygni (Ramularia leaf spots, physiological leaf spots) on barley and mint, and 7?. beticola on sugar beets; Rhizoctonia spp. on cotton, rice, potatoes, turf, corn, râpe, potatoes, sugar beets, vegetables and various other plants, e.g./?. solani (root and stem rot) on soybeans, R. solani (sheath blight) on rice and peanut, or R. cerealis (Rhizoctonia spring blight) on wheat or barley; Rhizopus stolonifer (black mold, soft rot) on strawberries, carrots, cabbage, vines and tomatoes; Rhynchosporium secalis (scald) on barley, rye and triticale; Sarocladium oiyzae and 5. atténuation (sheath rot) on rice; Sclerotinia spp. (stem rot or white mold) on vegetables and field crops, such as râpe, sunflowers (e.g.S. sclerotiorum) and soybeans (e.g.Y rolfsii or S. sclerotiorum), S. minor (sclerotinia blight) on peanut, 5. sclerotiorum (white mold) on potato; Sclerotium spp. on peanut (e.g. S. rolfsiiy, Septoria spp. on various plants, e.g.S. glycines (brown spot) on soybeans, S. tritici (Septoria blotch) on wheat and S. nodorum (syn. Stagonospora) (Stagonospora blotch) on cereals, S. avenue (septoria blotch) on oats, 5. linicola (pasmo) on flax seed; Uncinula (syn.Erysiphe) necator (powdery mildew, anamorph: Oïdium tuckeri) on vines; Setospaeria spp. (leaf blight) on corn (e.g.S. turcicum, syn. Helminlhosporium turcicum) and turf; Sphacelotheca spp. (smut) on corn, (e.g.S. reiliana: head smut), sorghum und sugarcane; Sphaerotheca fuliginea (powdery mildew) on cucurbits; Spongospora subterranea(powdery scab) on potatoes and thereby transmitted viral diseases; Stagonosporaspp. on cereals, e.g.S. nodorum (Stagonospora blotch, teleomorph: Leptosphaeria(syn. Phaeosphaeria)nodorum) on wheat, septrotia leaf and glume blotch on rye;Stemphyllium spp. e.g. stemphyllium leaf spot on coiton,Synchytrium endobioticum on potatoes (potatowart disease); Taphrina spp., e.g.T. déformons (leaf curl disease) on peachesand T. pruni (plum pocket) on plums; Thielaviopsis spp. (black root rot) on tobacco,pome fruits, vegetables, soybeans and cotton, e.g.T. basicola (syn. Chalara elegans);Tilletia spp. (common bunt or stinking smut) on cereals, such as e.g.T. tritici (syn. T.caries, wheat bunt) and T. controversa (dwarf bunt) on wheat; Typhula incarnata (grey snow mold) on barley or wheat; Urocystis spp., e.g.U. occulta (stem smut) on rye; Uromyces spp. (rust) on vegetables, such as beans (e.g.U. appendiculatus, syn. U. phaseoli) and sugar beets (e.g.U. betae): Ustilago spp. (loose smut) on cereals (e.g.U. nuda and U. avaenae), corn (e.g.U. maydis: corn smut) and sugar cane; Venturia spp. (scab) on apples (e.g.V. inaequalis) and pears; and Verticillium spp. (wilt) on various plants, such as fruits and ornamentals, vines, soft fruits, vegetables and field crops, e.g.V. dahliae on strawbemes, râpe, potatoes and tomatoes.

In a preferred embodiment, the présent invention provides a fungicidal composition comprising of tolfenpyrad and pyraclostrobin to control the undesired pathogenic microorganisms selected from the group comprising of, but not limited to, downy mildew, powdery mildew, black shank, black spot, melanose, post bloom fruit drop, anthracnose, alternaria brown spot, scab or greasy spot, leaf rust, black point, net blotch, scald, septoria leafand glume blotch, spot blotch, stem rust, stripe or yellow rust, yellow leaf spot, eyespot, gray leaf spot, northem corn leaf spot, physoderma brown spot, rust, Southern corn leaf blight, yellow leaf blight, leaf blast, or late blight.

The combination or the composition of the présent invention is also suitable for controlling the undesired pathogenic microorganisms occurring in plant parts, in the protection of materials (e.g. wood, paper, paint dispersions, fiber or fabrics) and in the protection of stored products. The term “protection of materials” is to be understood to dénoté the protection of technical and non-living materials, such as adhesives, glues, wood, paper and paperboard, textiles, leather, paint dispersions, plastics, cooling lubricants, fiber or fabrics, against the infestation and destruction by undesired pathological microorganisms, such as fungi and bacteria. As to the protection of wood and other materials, the particular attention is paid to the following undesired pathogenic microorganisms: Ascomycètes such as Ophiostoma spp., Ceratocystis spp., Aureobasidium pulhdans, Sclerophoma spp., Chaetomium spp., Humicola spp., Petriella spp., Trichunis spp.; Basidiomycetes such as Coniophora spp., Coriolus spp., Gloeophyllum spp., Lentinus spp., Pleurotus spp., Poria spp., Serpida spp. and Tyromyces spp., Deuteromycetes such as Aspergillus spp., Cladosporium spp., Pénicillium spp., Trichorma spp., Alternaria spp., Paecilomyces spp. and Zygomycetes such as Mucor spp., and in addition in the protection of stored products the following undesired pathogenic microorganisms are worthy of note: Candida spp. and Saccharomyces cerevisae.

The composition of the présent invention also provides a nonagronomic (other than field crops) application such as application on horticultural crops (e.g., greenhouse, nursery or ornamental plants not grown in a field), residential, commercial and industrial structures, turf (e.g., sod farm, pasture, golf course, lawn, sports field, etc.).

In an embodiment, the présent invention provides a method for controllinginsect pests and mites with an effective amount of the composition of tolfenpyrad and pyraclostrobin.

Insect pests or mites is selected from the order of moths and butterflies (Lepidoptera), beetles (Coleoptera),Oies, mosquitoes (Diptera), thrips (Thysanoptera), termites (Isopterd), cockroaches (Blattaria - Blaltoded), true bugs (Hemiptera), crickets, grasshoppers, locusts (Orthoptera), arachnids (Acarina), fleas (Siphonaptera), silverfish, firebrat (Thysanura), millipedes (Diplopoda), earwigs (Dermaptera), lice (Phthirapterd), or plant parasitic nématodes.

In afurther embodiment of the présent invention defines the insect pests and mites from the ordersof:

lepidopteians (Lepidoptera), for example Agrotis ypsilonfigrotis segetum (cutworm), Alabama argillacea, Anticarsia gemmatalis, Argyresthia conjugella,Autographa gamma, Bupalus piniarius, Cacoecia murinana, Capua reticulana, Cheimatobiabrumata, Choristoneura fumiferana, Choristoneura occidentalis, Cirphisunipuncta, Cydia pomonella, Cydia caryana (hickory shuckworm), Dendrolimus pini, Diaphania nitidalis, Diatraea grandosella, Earias insulana, Elasmopalpus lignosellus, Eupoecilia ambiguella, Evetria bouliana, Feltia subterranea, Galleria mellonella, Grapholitha funebrana, Grapholitha molesta,Heliothis armigera, Heliothis virescens(\.dbacco budworm), Heliothis zea, Hellula undalis, Hiberniadefoliaria, Hyphantria cunea, Hyponomeuta malinellus, Keiferia lycopersicella, Lambdina fiscellaria, Laphygma exigua, Leucoptera coffeella, Leucoptera scitella, Lithocolletis blancardella, Lobesia botrana, Loxostege sticticalis, Lymantria dispar, Lymantria monacha, Lyonelia clerkella, Malacosoma neustria, Mamestra brassicae, Orgyia pseudotsugata,Ostrinia nubilalisfeuropean corn borer), Panolis flammea, Pectinophora gossypiella(pmk bollworm), Peridroma saucia, Phalera bucephala, Phthorimaea operculella, Phyllocnistis citrella, Pieris brassicae, Pieris rapae (imported cabbageworm), Plathypena scabra, Plutella xylostella, Pseudoplusia includens, Rhyacionia frustrana, Scrobipalpula absoluta, Sitolroga cerealella, Sparganothis pilleriana, Spodoptera Jrugiperda(i'a\\ armyworm), Spodoptera littoralis, Spodoptera litura, Thaumatopoea pityocampa, Tortrix viridana, Trichoplusia ni, Zeiraphera canadensis, Giant swallowtail (orangedog), Paralobesia viteana (grape berry moth), Desmia funeralis (grape leaffolder),Helicoverpa zea (corn earworm), Helicoverpa armigeral (cotton bollworm), Agrotis segetum, Agrotis ipsilon, Agrotis exclamationis,Manduca quinquemaculata (tomato hornworm), Lithophane antennata (green fruitworm), Lithophane unimoda, Orthosia hibisci, Anarsia lineatella (peach twig borer), Acrobasis nuxvorella (pecan nut casebearer), Amyelois transitella (navel orangewoÎm),Marmara gulosa (Citrus peelminer)and Harrisina Americana (Grapeleaf skeletonizer), beetles (Coleoptera),fox example Agrilus sinuatus, Agriotes lineatus, Agriotes obscurus, Amphimallus solstitialis, Anisandrus dispar, Anthonomus grandis, Anthonomus pomorum, Aphthona euphoridae, Athous haemorrhoidalis, Atomaria linearis, Blastophagus piniperda, Blitophaga undata, Bruchus rufimanus, Bruchus pisorum, Bruchus lentis, Byctiscus betulae, Cassida nebtdosa, Cerotoma trifurcata, Cetonia aurata, Ceuthorrhynchus assimilis, Ceuthorrhynchus napi, Chaetocnema tibialis, Conoderus vespertinus, Crioceris asparagi, Ctenicera ssp., Curculio caryae (pecan weevil), Diabrotica longicornis, Diabrotica semipunctata, Diabrotica 12-punctata, Diabrotica speciosa, Diabrotica virgifera, Epilachna varivestis, Epitrix hirtipennis, Eutinobothrus brasiliensis, Hylobius abietis, Hypera brunneipennis, Hypera postica, Ips typographus, Lema bilineata, Lema melanopus, Leptinotarsa decemlineata(colorado potato beetle), Limonius californiens, Lissorhoptrus oryzophilus, Melanotus communis, Meligethes aeneus, Melolontha hippocastani, Melolontha melolontha,Ouléma oryzae, Ortiorrhynchus sulcatus, Otiorrhynchus ovatus, Phaedon cochleariae, Phyllobius pyri, Phyllotreta chrysocephala, Phyllophaga sp., Phyllopertha horticola, Phyllotreta nemorum, Phyllotreta striolala, Popillia japonicalfaganese beetle), Sitona lineatus, Sitophilus granaria, Diaprepes abbreviatus, and Conotrachelus nénuphar (plum curculio), flies, mosquitoes (Diptera), e.g. Aedes aegypti, Aedes albopictus, Aedes vexans, Anastrepha ludens, Anopheles maculipennis, Anopheles crucians, Anopheles albimanus, Anopheles gambiae, Anopheles freeborni, Anopheles leucosphyrus, Anopheles minimus, Anopheles quadrimaculatus, Calliphora vicina, Ceratitis capitata, Chrysomya bezziana, Chrysomya hominivorax, Chrysomya macellaria, Chrysops discalis, Chrysops silacea, Chrysops atlanticus, Cochliomyia hominivorax, Contarinia sorghicola Cordylobia anthropophaga, Cidicoides furens, Culex pipiens, Culex nigripalpus, Culex quinquefasciatus, Culex tarsalis, Culiseta inornata, Culiseta melanura, Dacus cucurbitae, Dacus oleae, Dasineura brassicae, Délia antique, Délia coarctata, Délia platura, Délia radicum, Dermatobia hominis, Drosophila suzukii (spotted wing drosophila),Fæwîzôz canicularis, Geomyza Tripunctata, Gasterophilus intestinalis, Glossina morsitans, Glossina palpalis, Glossina fuscipes, Glossina tachinoides, Haematobia irritans, Haplodiplosis equestris, Hippelates spp., Hylemyia platura, Hypoderma lineata, Leptoconops torrens, Liriomyza sativae, Liriomyza trifolii, Lucilia caprina, Lucilia cuprina, Lucilia sericata, Lycoria pectoralis, Mansonia titillanus, Mayetiola destructor, Musca domestica, Muscina stabulans, Oestrus avis, Oponiyza forum, Oscinella frit, Pegomya hysocyami, Phorbia antiqua, Phorbia brassicae, Phorbia coarctata, Phlebotomus argentipes, Psorophora columbiae, Psila rosae, Psorophora discolor. Prosimulium mixtum, Rhagoletis cerasi, Rhagoletis pomonellal (apple maggot), Sarcophaga haemorrhoidalis, Sarcophaga sp., Simulium vittatum, Stomoxys calcitrans, Tabanus bovinus, Tabanus atratus, Tabanus lineola, Tabanus similis, Tipula oleracea, Tipula paludosa, and Rhagoletis cerasi (cherry fruit fly),

Ümps(Thysanoptera), e.g. Dichromothrips corbetti, Dichromothrips ssp., Frankliniella fnsea,

Frankliniella occidentalis, Frankliniella tritici, Scirtothrips citri{C\tms thrips), Thrips oryzae, Thrips palmi, Thrips tabaci, and Drepanothrips reuleri (grape thrips) ténuités (Isoptera), e.g. Calotermes Jlavicollis, Leucotermes flavipes, Heterotermes aureus, Reticulitermes flavipes, Reticulitermes virginicus, Reticulitermes lucifugus, Termes natalensis, and Coptotermes formosanus, cockroaches (Blattaria - Blattodea), e.g. Blattella germanica, Blattella asahinae, Penplaneta americana, Periplaneta japonica, Periplaneta brunnea, Periplaneta fuligginosa,Periplaneta australasiae, and Blatta orientalis, true bugs (Hemiptera), e.g. Diaphorina citri, Acrosternum hilare, Blissns leiicopterus, Cyrtopeltis notatus, Dysdercus cingulatus, Dysdercus intermedius, Eurygaster integriceps, Euschistus impictiventris, Leptoglossus phyllopus, Lygus lineolaris, Lygiis pratensis, Nezara viridula, Piesma quadrata, Solubea insularis , Thyanta perditor, Acyrthosiphon onobrychis, Adelges laricis, Aphidula nasturtii, Aphis fabae, Aphis forbesi, Aphis pomi, Aphis gos sypii, Aphis grossulariae, Aphis schneideri, Aphis spiraecola, Aphis sambuci, Acyrthosiphon pisum, Aulacorthum solani, Bemisia argentifolii, Brachycaudus cardui, Brachycaudus helichrysi, Brachycaudus persicae, Brachycaudus prunicola, Brevicoryne brassicae, Capitophorus horni, Cerosipha gossypii, Chaetosiphon fragaefolii, Cryptomyzus ribis, Dreyfusia nordmannianae, Dreyfusia piceae, Dysaphis radicola, Dysaulacorthum pseudosolani, Dysaphis plantaginea, Dysaphis pyri, Empoasca fabae, Hyalopterus pruni, Hyperomyzus lactucae, Macrosiphum avenae, Macrosiphum euphorbiae, Macrosiphon rosae, Megoura viciae, Melanaphis pyrarius, Metopolophium dirhodum, Myzus persicae, Myzus ascalonicus, Myzus cerasi, Myzus varions, Nasonovia ribis-nigri, Nilaparvata lugens, Pemphigus bursarius, Perkinsiella saccharicida, Phorodon humuli, Psylla mail, Psylla piri, Rhopalomyzus ascalonicus, Rhopalosiphum maidis, Rhopalosiphum padi, Rhopalosiphum insertum, Sappaphis mala, Sappaphis mall, Schizaphis graminum, Schizoneura lanuginosa, Sitobion avenae, Trialeurodes vaporariorum,Toxoptera aurantiiaud, Viteus vitifolii, Cimex lectularius, Cimex hemipterus, Reduvius senilis, Triatoma spp., Cicadella viridis (green leafhoppers), Bactericera cockerelli (potato psyllid^/ri/us· critatus,Planococcus citri (citrus mea\ybug),Pseudococcus maritimus (grape me<Aybug),Pseudatomoscelis seriatus (cotton fleahopper),Proxys punctulatus (black stink bug), Halyomorpha halys,Citricola scale (Coccus pseudomagnoliarum), Bamacle sca\e(Ceroplastes cirripediformis), and Brown soft scale (Coccus hesperidum), ants, bees, wasps, sawflies (Hymenoptera), e.g. Athcdia rosae, Atta cephalotes, Atta capiguara, Atta cephalotes, Atta laevigata, Atta robusta, Atta sexdens, Atta texana, Cremalogasterspp., Hoplocampa minuta, Hoplocampa testudinea, Monomorium pharaonis, Solenopsis geminata, Solenopsis invicta, Solenopsis richteri, Solenopsis xyloni, Pogonomyrmex barbatus, Pogonomyrmex californiens, Pheidole megacephala, Dasymutilla occidentalis, Bombus spp. Vespula squamosa, Paravespula vulgaris, Paravespula pennsylvanica, Paravespula germanica, Dolichovespula maculata, Vespa crabro, Polistes rubiginosa, Camponotus floridanus, and Linepithema humile, crickets, grasshoppers, locusts (Orthoptera), e.g. Acheta domestica, Gryllotalpa gryllotalpa, Locusta migratoria, Melanoplus bivittatus, Melanoplus femurrubrum, Melanoplus mexicanus, Melanoplus sanguinipes, Melanoplus spretus, Nomadacris septemfasciata, Schistocerca americana, Schistocerca gregaria, Dociostaurus maroccanus, Tachycines asynamorus, Oedaleus senegalensis, Zonozerus variegatus, Hieroglyphus daganensis, Kraussaria angulifera, Calliptamus italiens, Chortoicetes terminifera, Scudderia spp. (katydid) and Locustana pardalina,

Arachnoidea, such as arachnids (Acarina), e.g. of the families Argasidae, Ixodidae and Sarcoptidae, such as Amblyomma americanum, Amblyomma variegatum, Ambryomma macidatum, Argas persicus, Boophilus annulatus, Boophilus decoloratus, Boophilus microplus, Dermacentor silvarum, Dermacentor andersoni, Dermacentor variabilis, Hyalomma truncatum, Ixodes ricinus, Ixodes rubicundus, Ixodes scapularis, Ixodes holocyclus, Ixodes pacifiais, Ornithodorus moubata, Ornithodorus hermsi, Ornithodorus turicata, Ornithonyssus bacoti, Otobius megnini, Dermanyssus gallinae, Psoroptes avis, Rhipicephalus sanguineus, Rhipicephalus appendiculatus, Rhipicephalus evertsi, Sarcoptes scabiei, and Eriophyidae spp. such as Aculus schlechtendali, PhyUocoptrataoleivora(c\ims rust mite), Aceria sheldoni (citrus bud mite)and Eriophyes sheldoni; Tarsonemidae spp. such as Phytonemus pallidus anâPolyphagotarsonemus lattis; Tenuipalpidae spp. such as Brevipalpus phoenicis; Tetranychidae spp. such as Tetranychus cinnabarinus, Tetranychus kanzawai, Tetranychuspacificus, Tetranychus telarius and Tetranychus urticae, Panonychus ulmi, Panonychuscitri(citms red mite), and Oligonychus pratensis; Araneida, e.g.

Lalrodectus mactans, and Loxoscelesreclusa, fleas (Siphonaptera), e.g. Ctenocephalides felis, Ctenocephalides canis, Xenopsylla cheopis, Pulex irritans, Tunga pénétrons, and Nosopsyllus fasciatus, silverfish, firebrat (Thysanura), e.g. Lepisma saccharina and Thermobia domestica, centipedes (Chilopoda), e.g. Scutigera coleoptrata, millipedes (Diplopoda), e.g. Narceus spp., earwigs (Dermaptera), e.g. forficula auricularia, lice (Phthiraptera), e.g. Pediculus humanus capitis, Pediculus humanus corporis, Pthirus pubis, Haematopinus eurystemus, Haematopinus suis, Linognathus vituli, Bovicola bovis, Menopon gallinae, Menacanthus stramineus and Solenopotes capillatus, plant parasitic nematodes such as root-knot nematodes, Meloidogyne arenaria, Meloidogyne chitwoodi, Meloidogyne exigua, Meloidogyne hapla, Meloidogyne incognita, Meloidogyne javanica and other Meloidogyne species; cyst nematodes, Globodera rostochiensis, Globodera pallida, Globodera tabacum and other Globodera species, Heterodera avenae, Heterodera glycines, Heterodera schachtii, Heterodera trifolii, and other Heterodera species; seed gall nematodes, Anguina funesta, Anguina tritici and other Anguina species; stem and foliar nematodes, Aphelenchoides besseyi, Aphelenchoides fragariae, Aphelenchoides ritzemabosi and other Aphelenchoides species; sting nematodes, Belonolaimus longicaudatus and other Belonolaimus species; pine nematodes, Bursaphelenchus xylophilus and other Bursaphelenchus species; ring nematodes, Criconema species, Criconemella species, Criconemoides species, and Mesocriconema species; stem and bulb nematodes, Ditylenchus destructor, Ditylenchus dipsaci, Ditylenchus myceliophagus and other Ditylenchus species; awl nematodes, Dolichodorus species; spiral nematodes, Helicotylenchus dihystera, Helicotylenchus multicinctus and other Helicotylenchus species, Rotylenchus robustus and other Rotylenchus species; sheath nematodes, Hemicycliophora species and Hemicriconemoides species; Hirshmanniella species; lance nematodes, Hoplolaimus columbus, Hoplolaimus galeatus and other Hoplolaimus species; false root-knot nematodes, Nacobbus aberrans and other Nacobbtts species; needle nematodes, Longidorus elongates and other Longidorus species; pin nematodes, Paratylenchus species; lésion nematodes, Pratylenchus brachyurus, Pratylenchus coffeae, Pratylenchus curvitatus, Pratylenchus goodeyi, Pratylencus neglectus, Pratylenchus pénétrons, Pratylenchus scribneri, Pratylenchus vidnus, Pratylenchus zeae and other Pratylenchus species; Radinaphelenchus cocophilus and other Radinaphelenchus species; burrowing nematodes, Radopholus similis and other Radopholus species; reniform nematodes, Rotylenchulus reniformis and other Rotylenchulus species; Scutellonema species; stubby root nematodes, Trichodorus primitivus and other Trichodorus species; Paratrichodorus minor and other Paratrichodorus species; stunt nematodes, Tylenchorhynchus claytoni, Tylenchorhynchus dubius and other Tylenchorhynchus species and Merlinius species; citrus nematodes, Tylenchulus semipenetrans and other Tylenchulus species; dagger nematodes, Xiphinema americanum, Xiphinema index, Xiphinema diversicaudatum and other Xiphinema species; and other plant parasitic nematode species.

In a preferred embodiment, the présent invention provides aninsecticidal combination/composition comprising of tolfenpyrad and pyraclostrobin to control the insect pests and mitesfrom the order of Lepidoptera, Coleoptera, Diptera, Hemiptera, Orthoptera, Acarina and Thysanoptera insects.

The composition of the présent invention used to control a wide variety of undesired pathogenic microorganisms, insect pests and mites, is advantageous, for several purposes, e.g.:

• useful for addressing a wider range of pest and diseases e.g. insecticidal, fungicidal and acaricidal activity;

• offering a single application as auniform composition in place of separate application of fungicides and insecticides;

• offering crop health improvement in comparison to separate applications of fungicides and insecticides;

• longer residual control after application of the composition.

In another embodiment, the présent invention providesa combination/composition that shows enhanced action against undesired pathogenic microorganisms, insect pests and mites, in comparison to the control rates that are possible with the individual compounds and/or suitable for improving the health of plants when applied to plants, parts of plants, plant propagation materials, or at their locus of their growth.

In yet another embodiment, the combination or the composition of the présent invention is particularly important for controlling a multitude of undesired pathogenic microorganisms, insect pests and mites, on various cultivated plants or plant parts, such as cereals, e.g. wheat, rye, barley, triticale, oats or rice; beet, e.g. sugar beet or fodder beet; fruits, such as pomes, stone fruits or soit fruits, e.g. apples, pears, plums, peaches, almonds, chemes, strawberries, raspberries, nectarine, prune, blackberries or gooseberries; leguminous plants, such as lentils, peas, alfalfa or soybeans; oil plants, such as râpe, mustard, olives, sunflowers, coconut, cocoa beans, castor oil plants, oil palms, ground nuts or soybeans; cucurbits, such as squashes, cucumber or melons; fiber plants, such as cotton, flax, hemp or jute; citrus fruit, such as oranges, lemons, grapefruits or mandarins; vegetables, such as spinach, lettuce, asparagus, cabbages, carrots, onions, tomatoes, potatoes, cucurbits or paprika; lauraceous plants, such as avocados, cinnamon or camphor; energy and raw material plants, such as corn, soybean, râpe, sugar cane or oil palm; corn; tobacco; nuts; coffee; tea; bananas; vines (table grapes and grape juice grape vines); hop; turf; sweet leaf (also called Stevia); natural rubber plants or ornamental and forestry plants, such as flowers, shrubs, broad-leaved trees or evergreens, e.g. conifers; and on the plant propagation material, such as seeds, and the crop material of these plants.

In a preferred embodiment, the combination or composition of the présent invention is particularly important for controlling a multitude of undesired pathogenic microorganisms, insect pests and mites, in the cucumber crop.

The rate of application amount varies depending on, for example, the blending ratio of an active ingrédient, a metrological condition, a dosage form, an application time, an application method, an application place, a pest to be controlled, and a target crop, in ordinary cases.

In the method of combating harmful fungi depending on the type of compound and the desired effect, the application rates of the mixtureaccording to the invention are , preferably from 5 to 1000 g/ha, most preferably from 10 to 750 g/ha, in particular from 20 to 500 g/ha.

In the method of combating pests (insects, acarids or nematodes) depending on the type of compound and the desired effect, the application rates of the mixture according to the invention are from , preferably from 20 to 1000 g/ha,more preferably from 10 to 750 g/ha, in particular from 20 to 500 g/ha.

In an embodiment, the présent invention provides a method for improving crop health (phytotonic effect), comprising treating a plant with an effective amount of composition of tolfenpyrad and pyraclostrobin.

The présent invention is further illustrated by the following examples. These examples describe possible preferred embodiments for illustrative purposes only, but they do not limit the scope ofthe invention.

Example 1: Emulsifiable concentrate (EC)

Ingrédients	% w/w	Function
Tolfenpyrad technical (97.0%)	15.5	Active ingrédient
Pyraclostrobintechnical (97.0%)	10.4	Active ingrédient
Polyoxyethylene alkyl ether	3.0	Emulsifier
Polyoxyethylene tristyrylphenyl ether	2.0	Emulsifier
Calcium alkylbenzene sulfonate	4.0	Emulsifier
N-Methyl-2-pyrrolidone	5.0	Solvent
Xylene	60.1	Solvent
Total	100.0

Préparation Method: Polyoxyethylene alkyl ether 3.0 parts, polyoxiethylene tristyrylppenol ether 2.0 parts and calcium alkylbenzene sulfonate wereput into a mixture of N-methyl-2pyrrolidone 5.0 parts and xylene 60.1 parts under mixing with an overhead stirrer and well mixed. Into this mixture, tolfenpyrad technical 15.5 parts and then pyraclostrobin technical 10.4 parts were added by portion wise. This mixture was well mixed till ail materials were completely solved.

Example 2: Oil-in water émulsion (EW)

Ingrédients	% w/w	Function
Tolfenpyrad technical (97.0%)	7.80	Active ingrédient
Pyraclostrobin technical (97.0%)	5.20	Active ingrédient
Polyoxyethylene castor oil ether	4.00	Emulsifier
Polyoxyethylene polyoxypropylene block copolymer	7.00	Emulsifier
Xanthan gum 1% aqueous solution	20.00	Thickener
Polydimethyl siloxane émulsion	0.10	Defoamer
1,2-Benzisothiazolin-3-one	0.05	Preservative

Xylene	15.00	Solvent
Polyethylene glycol	5.00	Anti-freezing agent
Water	35.85	Solvent
Total	100.0

Préparation Method: Tolfenpyrad technical 7.80 parts and pyraclostrobin 5.20 parts were completely solved into xylene 15.00 parts. Polyoxyethylene castor oil ether 4.00 parts, polyoxyethylene polyoxypropylene block copolymer 7.00 parts, polydimethyl siloxane émulsion 0.10 parts, l,2-benzisothiazolin-3-one 0.05 parts, polyethylene glycol 5.00 parts and water 35.85 parts were well mixed in a collodal mill. Into this mixture, the xylene solution of tolfenpyrad and pyraclostrobinwas put portion wise and emulsified till the médian diameter of oil droplet becomes below 2 pm. Into the prepared emulstion mixture, xanthan gum 1% aqueous solution 20.00 parts was added and mixed uniformly.

Example 3: Wettable Powder (WP)

Ingrédients	% w/w	Function
Tolfenpyrad technical (97.0%)	15.5	Active ingrédient
Pyraclostrobin technical (97.0%)	10.4	Active ingrédient
Sodium alkyl sulfate	3.0	Wetting Agent
Sodium lignin sulfonate	2.0	Wetting Agent
Sodium naphthalene sulfonate formaldéhyde condensate	4.0	Dispersing Agent
Precipitated silica	6.0	Carrier
Kaolin	59.1	Carrier
Total	100.0

Préparation Method: Kaolin 59.1 parts and precipitated silica 6.0% were well mixed in a high shear mixer. After mixing well, tolfenpyrad technical 15.5 parts, pyroxastrobin technical 10.4 parts, sodium alkyl sulfate 3.0 parts, sodium lignin sulfonate 2.0 parts and sodium naphthalene sulfonate formaldéhyde condensate 4.0 parts were added and mixed well in a high shear mixer. The mixture was milled by an air jet mill under operaton condition to get the médian diameter below 1 Opm.

Example 4:Water dispersible granule (WG)

Ingrédients	% w/w	Function
Tolfenpyradtechnical (97.0%)	31.0	Active ingrédient
Pyraclostrobin technical (97.0%)	20.6	Active ingrédient
Sodium naphthalene sulfonate formaldéhyde condensate	3.0	Dispersing Agent
Sodium lignin sulfonate	8.0	Dispersing Agent

Sodium alkyl sulfonate	2.0	Wetting agent
_Fumedsilica	2.0	Carrier
Ammonium sulfate	16.7	Cartier
Kaolin	16.7	Carrier
Total	100.0

Préparation Method: Ammonium sulfate 16.7 parts, kaolin 16.7 parts and fumed silica 2.0% were well mixed in a high shear mixer. After mixing well, tolfenpyrad technical 31.0 parts, pyroxastrobin technical 20.6 parts, sodium naphthalene sulfonate formaldéhyde condensate 3.0 parts, sodium lignin sulfate8.0 parts andsodium alkyl sulfonate2.0 parts and were added, and mixed well in a high shear mixer. The mixture was milled by an air jet mill under operaton condition to get the médian diameter below 10 pm.The resulting mixture was kneaded with water, extruded through a screen (0.8 mm diameter opening) and dried.

Example 5: Suspension Concentrate (SC)

Ingrédients	% w/w	Function
Tolfenpyrad technical (97.0%)	15.50	Active ingrédient
Pyraclostrobin technical (97.0%)	10.40	Active ingrédient
Polyoxyethylene alkyl ether	3.00	D i sp ers ing/W etting Agent
Polymethacrylate-polyoxy ethylene graft copolymer	4.00	Dispersing Agent
Sodium naphthalene sulfonate formaldéhyde condensate	1.00	Dispersing Agent
Xanthan gumaqueous 1 % solution	15.00	Thickener
1,2-BenzisothiazoIin-3-one	0.05	Preservative
Polyethylene glycol	6.00	Antifreezing agent
Polysiloxane émulsion	0.30	Defoamer
Distilled water	44.75	Solvent
Total	100.00

Préparation Method: Polyoxyethylene alkyl ether 3.00 parts, polymethacrylate-polyoxy ethylene graft copolymer 4.00 parts, sodium naphthalene sulfonate formaldéhyde condensate 1.00 parts, l,2-benzisothiazolin-3-one 0.05 parts and polysiloxane émulsion 0.30 parts were mixed into a solution of distilled water 44.75 parts and polyethylene glycol 6.00 parts. Into this mixture, tolfenpyrad technical 15.50 parts and pyraclostrobin technical 10.40 were added and pre-milled in a colloïdal mill till the médian diameter of tolfenpyrad technical and pymetrozine technical become below 100 micrometers.The pre-milled mixture was milled in a bead mill till the médian diameter of the technicals become below 5 pm. After milling, xanthan gum aqueous 1% solution 15.00 parts is added into the milled solution and mixed uniformly.

Following the test results illustrate the présent invention and are not intended to limit the présent invention.

Example: Biological effect of Combination of Tolfenpyrad and Pyraclostrobin:

Experiments were carried out to study the biological efficacy in terms of disease, pest control, crop vigour, and yield with the combinations of the présent invention. Bio-efficacy assessments were done for control of Downey mildew, Powdery mildew, Jassid and thrips in cucumber and its indirect influence on cucumber vigour and yield.

S. No.	Product/ Compound	Formulation Type	Dose g a.i./ha
1	Tolfenpyrad	AI	120
2	Tolfenpyrad	AI	150
3	Pyraclostrobin	AI	80
4	Pyraclostrobin	AI	100
5	Tolfenpyrad + Pyraclostrobin	AI	120+80
6	Tolfenpyrad + Pyraclostrobin	AI	120+100
7	Tolfenpyrad + Pyraclostrobin	AI	150+80
8	Tolfenpyrad + Pyraclostrobin	AI	150+100
9	Untreated
	*Combinations are tank mix

Trials were conducted in open field to test the efficacy of the combination of tolfenpyrad and pyraclostrobin for controlling downy mildew, powdery mildew, thrips and jassid in cucumber at different test doses.

The test compounds were applied 30 days after sowing at the given application rates with the help of back pack sprayers fitted with a hollow cône nozzle. The water volume used for spraying was 5001ts/ha, the size of each plotwas 50 sq. mt and each treatment was replicated 3 times.

Disease and pest severity was recorded one day before application and 10 days post application. Crop vigour was recorded at 30 days after application. The cucumber was harvested at a weekly interval based on the maturity index of green cucumber. Six picking yield was recorded.

At application time the cucumber crop (Cucumber) indicated 8% downy mildew and 10% powdery mildew severity, while the mean pre-count of jassids was 3/leaf and thrips was 5/leaf. At data recording time, the disease severity of downy mildew was 25% and of powdery mildew was 35% in the untreated plots while the jassid population was 8/leaf and thrips population was 15/leaf at data recording. The composition comprising the compound of component (1) and the component (2) shows a synergistic effect. According to the présent invention, a synergistic effect of the combination is always présent when the pesticidal activity of the active compound combinations exceeds the total of the activities of the single combining partners when applied individually. The expected activity for a given combination of two active compounds (binary mixture) can be calculated by using Colby’s formula (COLBY, S.R. “Calculating synergistic and antagonistic responses of herbicide combination”, Weeds, Vol. 15, pages 20-22; 1967).

Colby's formula:

The expected activity for a given combination of two active compounds (binary composition) can be calculated as follows:

XY ïôo

In which E represents the expected percentage of inhibition of the disease for the combination of two pesticides at defined doses (for example equal to x and y respectively), x is the percentage of inhibition observed for the disease by the compound (1) at a given dose (equal to x), y is the percentage of inhibition observed for the disease by the compound (2) at a defined dose (equal to y). When the percentage of inhibition observed for the combination is greater than E, there is a synergistic effect.

Here the efficacy is determined in %. 0% means an efficacy which corresponds to that of the control; whereas 100 % means that no infection is observed.

If an actual combination exceeds the calculated value, the action of the combination is super additive i.e., a synergistic effect is présent, in this case, the actually observed efficacy must exceed the value calculated using the above formula for expected efficacy (E). In the case of purely complementary addition of activities (expected activity), said différence (O-E) is zéro. A négative value of the différence (O-E) signais a loss of activity compared to the expected efficacy. However, besides the actual synergistic action with respect to fungicidal activity, the composition according to the invention can also hâve further surprising advantageous properties. Examples of such advantageous properties that may be mentioned are more advantageous degradability; improved toxicological and ecotoxicological behaviours; or improved characteristics of the useful plants including, emergence,crop yields, more developed root system, tillering increases, increase in plant height, bigger leaf blades, stronger tillers, greener leaf colours, early flowering, early maturity, increased shoot growth, increased plant vigour and early germination.

The agrochemical composition of the présent invention also exhibits vigourous growth in crop. Vigour, here refers to early leaf area development and growth rate of the crop, has often been associated with improved compétitive ability. Rapid canopy closure allows crops to quickly shade the ground and competing plants. Vigourous early growth may also promote greater root growth and improve water-use efficiency (Siddique et al., 1990; Richards et al., 2007; Richards, 2008), which may contribute to higher yields in water and nutrient-limited environments overall a potent strengthening effect in plants is observed. Accordingly, they can be used for mobilizing the defenses of the plant against attack by undesirable microorganisms. Increased plant vigour, comprising plant health/plant quality and seed vigour, reduced stand failure, improved appearance, increased recovery after periods of stress, improved pigmentation (e.g. chlorophyll content, stay-green effects, etc.) and improved photosynthetic efficiency.

Table 1 : Vigour index followed

Under optimal conditions, more fruit may initially develop from the axil of 4 per leaf than can later be supported to full size, so fruit may need thinning. Plants allowed to bear too much fruit become exhausted, abort fruit, and fluctuate widely in productivity over time. Excessive plant vigor is indicated by rapid growth, thick and brittle stems, large leaves, long tendrils, deep green foliage, the profusion of fruit, and large, deep yellow flowers. On the other hand, cucumbers are very sensitive to unfavorable conditions, and the slightest stress affects their growth and productivity.

Score	Description
1	Very thin and tender stem & stunted tendrils, very small yellowish leaf, very light yellow flower, very low fruit growth, very poor vigour

2	Thin and soit stem, stunted tendrils, small yellowish leaf, very light yellow flower, low fruit growth, poor vigour
3	Thin and soft stem, short tendrils, green and moderate leaf, moderately light yellow flower, low moderate fruit growth, low vigour
4	Moderately thick stem, short tendrils, green and moderate leaf, moderately light yellow flower, moderate fruit growth, fairly low vigour
5	Moderately thick stem, moderately tendrils, moderately large and green leaf, light yellow flower, moderate fruit growth, moderate vigour
6	Moderately thick and brittle stem, moderately tendrils, moderately large and slightly pale leaf, light yellow flower, good fruit growth, fairly high vigour
7	Thick and brittle stem, average length tendrils, moderately large and green leaf, yellow flower, good fruit growth, high vigour
8	Thick and brittle stem, long tendrils large and green leaf, yellow flower, excellent fruit growth, very high vigour
9	Very thick and very brittle stem long tendrils, very large and green leaf, deep yellow flower and profuse fruit growth, excellent vigour

Effects of the combinations on disease control, pest control, vigour and yield of cucumber are given in the following tables.

Table 2: Activity of the combination on Downey mildew of Cucumber

SN	Product/ Compound	Formulation type	Dose g a.i./ha	Mean % control 10 Days after Application	E = (X+Y)(X*Y)/ 100	O-E
A	B		RI	R2	R3	Mean
1	Tolfenpyrad	AI	120	60	62	64	62
2	Tolfenpyrad	AI	150	66	64	65	65
3	Pyraclostrobin	AI	80	68	69	70	69
4	Pyraclostrobin	AI	100	73	74	77	75
5	Tolfenpyrad + Pyraclostrobin	AI	120+80	97	94	94	95	88.22	6.78
6	Tolfenpyrad + Pyraclostrobin	AI	120+10 0	100	100	100	100	90.5	9.5
7	Tolfenpyrad + Pyraclostrobin	AI	150+80	86	92	92	90	89.15	0.85
8	Tolfenpyrad + Pyraclostrobin	AI	150+10 0	100	100	100	100	91.25	8.75

It was thus concluded that the combinations of the présent invention were surprisingly synergistically efiîcacious for the control of downy mildew.

I able 3: Activity of the combination on Powdery mildew of Cucumber

SN	Product/ Compound	Formulation Type	Dose g a.i./ha	Mean % control 10 Days after application	E =(X+Y)(X*Y)/100	O-E
	A	B		RI	R2	R3	Mean
1	Tolfenpyrad	AI	120	54	54	57	55
2	Tolfenpyrad	AI	150	66	65	67	66
3	Pyraclostrobin	AI	80	60	59	64	61
4	Pyraclostrobin	AI	100	73	69	71	71
5	Tolfenpyrad + Pyraclostrobin	AI	120+80	81	88	86	85	82.45	2.55
6	Tolfenpyrad + Pyraclostrobin	AI	120+100	89	89	92	90	86.95	3.05
7	Tolfenpyrad + Pyraclostrobin	AI	150+80	93	91	86	90	86.74	3.26
8	Tolfenpyrad + Pyraclostrobin	AI	150+100	97	94	94	95	90.14	4.86

It was thus concluded that the combinations of the présent invention were surprisingly synergistically active for the control of Powdery mildew.

Table 4: Activity of the combination on Jassid of Cucumber

SN	Product/ Compound	Formulation Type	Dose g a.i./ha	Mean % control 10 Days after Application	E = (X+Y)(X*Y)/100	O-E
A	B		RI	R2	R3	Mean
1	Tolfenpyrad	AI	120	76	70	79	75
2	Tolfenpyrad	AI	150	81	85	83	83
3	Pyraclostrobin	AI	80	0	0	0	0
4	Pyraclostrobin	AI	100	0	0	0	0
5	Tolfenpyrad + Pyraclostrobin	AI	120+80	75	78	72	77	75	2
6	Tolfenpyrad + Pyraclostrobin	AI	120+100	85	78	77	80	75	5
7	Tolfenpyrad + Pyraclostrobin	AI	150+80	86	89	89	88	83	5
8	Tolfenpyrad + Pyraclostrobin	AI	150+100	88	92	90	90	83	7
9	Untreated (Jassid count/leaf)			9	7	8	8

It was thus concluded that the combinations of the présent invention surprisingly indicated jassids control synergies.

Table 5: Activity of the combination on Thrips of Cucumber

SN	Product/ Compound	Formulation Type	Dose g a.i./ha	Mean % control 10 Days after Application	E = (X+Y)(X*Y)/100	O-E
A	B		RI	R2	R3	Mean
1	Tolfenpyrad	AI	120	71	73	72	72
2	Tolfenpyrad	Al	150	82	86	87	85
3	Pyraclostrobin	AJ	80	0	0	0	0
4	Pyraclostrobin	AI	100	0	0	0	0
5	Tolfenpyrad + Pyraclostrobin	Al	120+80	78	74	73	75	72	3
6	Tolfenpyrad + Pyraclostrobin	AI	120+10 0	79	75	74	76	72	4
7	Tolfenpyrad + Pyraclostrobin	AI	150+80	89	91	90	90	85	5
8	Tolfenpyrad + Pyraclostrobin	AI	150+10 0	94	90	89	91	85	6
9	Untreated (thrips count/leaf)			16	15	14	15

Synergies in terms of thrips control with the said combinations are clearly reflected in the data.

Table 6: Activity of the combination on crop vigour

SN	Product/ Compound	Formulation Type	Dose g a.i./ha	Crop vigour 30 days after application	(%) increas e over control	E =(X+Y) (X*Y)/ 100 (%)	O-E (%)
A	B		RI	R2	R3	Mean
1	Tolfenpyrad	AI	120	4.1	4.1	4.4	4.2	40.00
2	Tolfenpyrad	AI	150	4.6	4.5	4.7	4.6	53.33
3	Pyraclostrobin	AI	80	5.8	6.0	6.2	6	100.00
4	Pyraclostrobin	AI	100	6.4	6.3	6.2	6.3	110.00
5	Tolfenpyrad + Pyraclostrobin	AI	120+80	6.5	6.5	6.8	6.6	120.00	100.00	20.00
6	Tolfenpyrad + Pyraclostrobin	AI	120+10 0	6.9	6.8	7.0	6.9	130.00	106.00	24.00
7	Tolfenpyrad + Pyraclostrobin	AI	150+80	7.2	7.3	7.6	7.4	146.67	100.00	46.67
8	Tolfenpyrad + Pyraclostrobin	AI	150+10 0	8.0	7.8	7.9	7.9	163.33	104.67	58.67
9	Untreated			2.7	3.1	3.2	3

It was thus concluded that the cucumber crop treated with the test combinations according to the présent invention surprisingly indicated synergies in terms of vigour which could be causedby the effective disease control, pest control and the activation of some bénéficiai physiological pathways. Test combination of Tolfenpyrad + Pyraclostrobin @ 150+100 & 80 ga.i./ha indicated the highest synergies in terms of crop vigour. Delayed onset of senescence in the combination plots was one of the key observations at maturity which led to an extended crop cycle of 10 days.

Table 7: Activity of the combination on yield of Cucumber

SN	Product/ Compound	Formulation type	Dose g a.i./ha	Yield (Mean of 6 pickings)	E =(X+Y)- (X*Y)/100 (%)	O-E (%)
A	B	A	RI	R2	R3	Yield Quintal/ha	O=Yield Synergy (%)
1	Tolfenpyrad	AI	120	31.6	31.2	31.4	31.4	4.67
2	Tolfenpyrad	AI	150	32.1	32.2	32.3	32.2	7.33
3	Pyraclostrobin	AI	80	32.9	32.9	32.6	32.8	9.33
4	Pyraclostrobin	AI	100	33.6	33.9	33.6	33.2	10.67
5	Tolfenpyrad + Pyraclostrobin	AI	120+80	34.9	35.2	35.2	35.1	17.00	13.56	3.4
6	Tolfenpyrad + Pyraclostrobin	AI	120+100	36.0	35.9	35.8	36.7	22.33	14.84	7.4
7	Tolfenpyrad + Pyraclostrobin	AI	150+80	38.1	38.0	38.2	38.7	29.00	15.98	13.0
8	Tolfenpyrad + Pyraclostrobin	AI	150+100	39.4	39.1	39.1	40	33.33	17.22	16.1
9	Untreated			29.0	32.0	29.0	30

Synergies seen in terms of increased yield can be attributed to better pest and disease control and delayed senescence.

Thus, from the foregoing description, it will be apparent to one of the person skilled in the art that many changes and modifications can be made thereto without departing from the spirit or scope of the invention as set forth in the description. Accordingly, it is not intended that the scope ofthe foregoing description be limited to the description set forth above, but rather that 20 such description be construed as encompassing such features that résidé in the présent invention, including ail the features and embodiments that would be treated as équivalents thereof by those skilled in the relevant art.

Claims

Claims:

1. A composition comprising of

a) an effective amount of tolfenpyrad or salt(s) thereof,

b) an effective amount of pyraclostrobin or salt(s) thereof,

c) optionally, an addition of one or more insecticidal or fungicidal or acaricidal or nematicidal or herbicidal or plant growth regulator compounds or any combination thereof,

d) agriculturally acceptable additives.
2. A synergistic composition comprising of

a) an effective amount of tolfenpyrad or salt(s) thereof,

b) an effective amount of pyraclostrobin or salt(s) thereof,

c) optionally, an addition of one or more insecticidal or fungicidal or acaricidal or nematicidal or herbicidal or plant growth regulator compounds or any combination thereof,

d) agriculturally acceptable additives.
3. The composition as claimed in claim 1, wherein said composition comprises of weight percentage of tolfenpyrad is about 4% to 80%, preferably about 10% to 60%, the weight percentage of pyraclostrobin is about 80% to 4%, preferably about 60% to 10% and the weight percentage of additives is about 5% to 95%, preferably about 20% to 90%.
4. The composition as claimed in claim 1, wherein the tolfenpyrad and pyraclostrobin in a ratio 50:1 to 1:50, preferably 20:1 to 1:20 or 10:1 to 1:10, more preferably 3:2 to 2:3.
5. The composition as claimed in claim 1, wherein said composition is formulated as a wettable powder (WP), a water dispersible granule (WDG), a water dispersible powder, a water dispersible tablet (WT), a suspension concentrate (SC), an ultra-low volume (ULV) liquid, an ultralow volume (ULV) suspension (SU), a water soluble granule (SG), an emulsifiable granule (EG), an emulsifiable powder (EP), a dispersible concentrate (DC), an oil-in water émulsion (EW), an emulsifiable concentrate (EC), a micro-emulsion (ME), an oil dispersion (OD), a capsule suspension (CS), a dustable powder (DP) or an aérosol.
6. The composition as claimed in claim 1, wherein said composition further comprises of agriculturally acceptable formulation additives is selected from the group comprising of surfactant(s), liquid carrier(s), solid carrier(s), binder(s), disintegrating agent(s), pH adjuster(s), thickener(s), preservative(s), antifreezing agent(s), defoamer(s), coloring agent(s), or a combination thereof.
7. The composition as claimed in claim 6, wherein suitable surfactant(s) is selected from the group comprising of sorbitan monolaurate, polyoxyethylene sorbitan monolaurate; alkyl polyglucoside (decyl glucoside), polyoxyethylene alkyl ethers (polyoxyethylene lauryl ether, or polyoxyethylene coconut fatty alcohol ether), polyoxyethylene alkynyl ether (polyoxyethylene 2,4,7,9-tetramethyl-5-decyn-4,7-diol ether), polyoxyethylene aryl ethers (polyoxyethylene nonylphenyl ether or polyoxyethylene tristyrylphenyl ether), polyoxyethylene vegetable oil ethers (polyoxyethylene castor oil or polyoxyethylene hydrogenated castor oil), vegetable oil ethoxylate, alcohol C12-C15 ethoxylate; polyoxyethylene fatty acid esters(polyoxyethylene monolaurate, polyoxyethylene distearate or polyoxyethylene resin acid ester); polyoxyethylene polyoxypropylene (EO-PO) block co-polymers (Pluronic®), polyoxyethylene polyoxypropylene alkyl ether (polyoxyethylene polyoxypropylene lauryl ether), polyoxyethylene polyoxypropylene aryl ether (polyoxyethylene polyoxypropylene styrylphenyl ether), polyoxyethylene alkyl amines (polyoxyethylene stearyl amine), polyoxyethylene fatty acid amide (lauric acid diethanolamid), fluorinated surfactant, alkyl sulfates (sodium lauryl sulfate), sodium alkylbenzene sulfonate, calcium alkylbenzene sulphonate, polyoxyethylene alkyl ether sulfates (sodium polyoxyethylene lauryl ether sulfate), polyoxyethylene aryl ether sulfates (sodium polyoxyethylene nonylphenyl ether sulfate or ammonium polyoxyethylene tristyrylphenyl ether sulfate), aryl sulfonate (calcium dodecyl benzene sulfonate, sodium naphthalene sulfonate or sodium naphthalene sulfonate formaldéhyde condensate), poly aryl phenyl ether sulphate ammonium sait, ‘alpha’-olefin sulfonate, alkyl sulfosuccinate (sodium dioctyl sulfosuccinate), sodium lignosulfonate,polycarboxylic acid sodium sait, N-methyl fatty acid sarcosinate, polyoxyethylene alkyl ether phosphate, polyoxyethylene aryl ether phosphates(polyoxyethylene phenyl ether phosphate), polyoxyethylene alkyl phenyl ether phosphate, graft co-polymers (polymethyl methacrylate-polyethylene glycol graft copolymer), or a combination thereof.
8. The composition as claimed in claim 6, wherein the suitable liquid carrier(s) is selected from the group comprising of water, alcohols (éthanol, propanol, butanol, noctanol, isopropanol ethylene glycol, diethylene glycol, propylene glycol, polyethylene glycol, benzyl alcohol, glycerin), polyol ethers (ethylene glycol monopropyl ether, diethylene glycol monomethyl ether, dipropylene glycol dimethyl ether), ketones (acetone, methyl ethyl ketone, methyl isobutyl ketone, cyclohexanone; ethers such as dipropyl ether, dioxane, tetrahydrofuran), aliphatic hydrocarbons (normal paraffin, isoparaffin, kerosene, minerai oil), aromatic hydrocarbons (xylene, toluene, naphthene, solvent naphtha, solvent C9, solvent Cio, solvent C12, solvesso 100, solvesso 150, solvesso 200), chlorinated aliphatic or aromatics hydrocarbons (chlorobenzene, chloroethylene, methylene chloride); esters (ethyl acetate, diisopropyl phthalate, dimethyl adipate, methyl oleate, methyl tallowate), lactones (gammabutyrolactone, gamma-valerolactone, epsilon-caprolactone), amides (dimethylformamide, N-methylpyrrolidone, N-octylpyrolidone, N,Ndimethyldecanamide), nitriles (acetonitriie); organosulfur compound (dimethyl sulfoxide), vegetable oils (soybean oil, rapeseed oil, cotton seed oil) or a combination thereof.
9. The composition as claimed in claim 6, wherein suitable solid camer(s) is selected from the group comprising of natural minerais (quartz, talc, kaolinite, pyrophyllite, montmorillonite, attapulgite, bentonite, kieselguhr, chalk, zeolite, calcite, sericite, acid clay, diatomaceous earth, Fuller’s earth, or pumice), synthetic minerais (precipitated silica, fumed silica and calcium carbonate), inorganic salts (calcium carbonate, ammonium sulfate, sodium sulfate, potassium chloride), organic materials (urea, lactose, starch, lignin, cellulose, cottonseeds hulls, wheat flour, soyabean flour, wood flour, walnut shell flour, plant powders), plastic carrier polyethylene, polypropylene or polyvinylidene chloride or a combination thereof.
10. The composition as claimed in claim 6, wherein suitable binder(s) is selected from the group comprising of polyvinyl alcohol, dextrin, denatured dextrin, soluble starch, guar gum, sucrose, polyvinylpyrrolidone, gum arabic, polyvinyl acetate, polysodium acrylate, carboxymethyl cellulose or its sait, carboxymethylcellulose dextrin, bentonite, polyethylene glycol having an average molecular weight of 6,000 to

20,000, polyethylene oxide having average molecular weight of 100,000 to 5,000,000, natural phosphatide (cephalinic acid or lecithin) or a combination thereof.
11. The composition as claimed in claim 6, wherein suitable disintegrating agent(s) is selected from the group comprising of sodium tripolyphosphate, stearic acid métal sait, cellulose powder, dextrin, méthacrylate copolymer, polyvinylpyrrolidone, polyaminocarboxylic acid chelate compound, styrene sulfonate/isobutylene/maleic anhydride copolymer, starch/polyacrylonitrile graft copolymer, sodium hexametaphosphate, carboxymethyl cellulose, sodium polycarbonate, bentonite or a combination thereof.
12. The composition as claimed in claim 6, wherein suitable pH adjuster(s) is selected from the group comprising of sodium or potassium carbonate, sodium or potassium hydrogen carbonate, sodium or potassium dihydrogenphosphate, disodium or dipotassium hydrogenphosphate, citric acid, malic acid and triethanolamine, or a combination thereof.
13. The composition as claimed in claim 6, wherein suitable thickener(s) is selected from the group comprising of xanthan gum, welan gum, guar gum, polyvinyl alcohol, carboxylmethylcellulose, polyvinylpyrrolidone, carboxyvinyl polymer, acrylic polymer, starch dérivative or polysaccharide, silica, bentonite, or white carbon or a combination thereof.
14. The composition as claimed in claim 6, wherein suitable preservative(s) is selected from the group comprising of 4-hydroxybenzoic acid esters, 2-methyl-4-isothiazolin3-one, 5-chloro-2-methyl-4-isothiazolin-3-one and l,2-benzisothiazolin-3-one, or a combination thereof.
15. The composition as claimed in claim 6, wherein suitable anti-freezing agent(s) is selected from the group comprising of glycerin, ethylene glycol, polyethylene glycol, propylene glycol, diethylene glycol, dipropylene glycol and urea, or a combination thereof.
16. The composition as claimed in claim 6, wherein suitable defoamer(s) is selected from the group comprising of silicone compounds and organic fluorine compounds, or a combination thereof.
17. The composition as claimed in claim 6, wherein suitable coloring agent(s) is selected from the group comprising of organic dyestuffs (azo dye, phthalocyanine dye or alizarin dye) and inorganic pigments (iron oxide), or a combination thereof.
18. A method for controlling pest(s), wherein the pest, their habitat, breeding grounds, their locus, or the plants to be protected against pest attack, the soil or plant propagation material are treated with an effective amount of the composition of claim
19. A method for controlling phytopathogenic harmful fungi, comprising treating a fungus, a habitat of the fungus, a seed, soil, or a plant to be protected against fungal attack with an effective amount of the composition of claim 1.
20. A method for improving plant health, comprising treating a plant propagation material of the plant, or a locus where the plant is growing or is to grow with an effective amount of composition of claim 1.
21. A process for preparing the composition of claim 1, comprising mixing the tolfenpyrad and pyraclostrobin with agriculturally acceptable formulation additives.