NZ749305A - Rna-guided human genome engineering - Google Patents
Rna-guided human genome engineeringInfo
- Publication number
- NZ749305A NZ749305A NZ749305A NZ74930513A NZ749305A NZ 749305 A NZ749305 A NZ 749305A NZ 749305 A NZ749305 A NZ 749305A NZ 74930513 A NZ74930513 A NZ 74930513A NZ 749305 A NZ749305 A NZ 749305A
- Authority
- NZ
- New Zealand
- Prior art keywords
- rna
- cas9
- human genome
- genome engineering
- guided human
- Prior art date
Links
- 238000010362 genome editing Methods 0.000 title 1
- 108091033409 CRISPR Proteins 0.000 abstract 3
- 108020005004 Guide RNA Proteins 0.000 abstract 2
- 101710163270 Nuclease Proteins 0.000 abstract 2
- 108091027544 Subgenomic mRNA Proteins 0.000 abstract 2
- 210000003527 eukaryotic cell Anatomy 0.000 abstract 2
- 230000000295 complement effect Effects 0.000 abstract 1
- 102000034287 fluorescent proteins Human genes 0.000 abstract 1
- 108091006047 fluorescent proteins Proteins 0.000 abstract 1
- 230000004927 fusion Effects 0.000 abstract 1
- 150000007523 nucleic acids Chemical group 0.000 abstract 1
- 108091006106 transcriptional activators Proteins 0.000 abstract 1
- 108091006107 transcriptional repressors Proteins 0.000 abstract 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention provides a method of altering a eukaryotic cell comprising providing to the eukaryotic cell a plurality of crRNA-tracrRNA fusion guide RNA sequences (sgRNAs) complementary to different target nucleic acid sequences and a Cas9 protein which interacts with the guide RNA sequences. The invention also provides the use of a single sgRNAs, a nuclease null Cas9 and Cas9 modified to include a FokI nuclease domain, a transcriptional activator/repressor or a fluorescent protein.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261738355P | 2012-12-17 | 2012-12-17 | |
| US201361779169P | 2013-03-13 | 2013-03-13 | |
| NZ709429A NZ709429B2 (en) | 2012-12-17 | 2013-12-16 | Rna-guided human genome engineering |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| NZ749305A true NZ749305A (en) | 2020-06-26 |
Family
ID=72291340
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NZ749305A NZ749305A (en) | 2012-12-17 | 2013-12-16 | Rna-guided human genome engineering |
| NZ749319A NZ749319A (en) | 2012-12-17 | 2013-12-16 | Rna-guided human genome engineering |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NZ749319A NZ749319A (en) | 2012-12-17 | 2013-12-16 | Rna-guided human genome engineering |
Country Status (1)
| Country | Link |
|---|---|
| NZ (2) | NZ749305A (en) |
-
2013
- 2013-12-16 NZ NZ749305A patent/NZ749305A/en unknown
- 2013-12-16 NZ NZ749319A patent/NZ749319A/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| NZ749319A (en) | 2020-06-26 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PSEA | Patent sealed | ||
| RENW | Renewal (renewal fees accepted) |
Free format text: PATENT RENEWED FOR 1 YEAR UNTIL 16 DEC 2021 BY COMPUTER PACKAGES INC Effective date: 20201201 |
|
| RENW | Renewal (renewal fees accepted) |
Free format text: PATENT RENEWED FOR 1 YEAR UNTIL 16 DEC 2022 BY COMPUTER PACKAGES INC Effective date: 20211130 |
|
| RENW | Renewal (renewal fees accepted) |
Free format text: PATENT RENEWED FOR 1 YEAR UNTIL 16 DEC 2023 BY COMPUTER PACKAGES INC Effective date: 20221130 |
|
| RENW | Renewal (renewal fees accepted) |
Free format text: PATENT RENEWED FOR 1 YEAR UNTIL 16 DEC 2024 BY COMPUTER PACKAGES INC Effective date: 20231130 |