NZ744434A - Methods and compositions for preventing norleucine misincorporation into proteins - Google Patents
Methods and compositions for preventing norleucine misincorporation into proteinsInfo
- Publication number
- NZ744434A NZ744434A NZ744434A NZ74443413A NZ744434A NZ 744434 A NZ744434 A NZ 744434A NZ 744434 A NZ744434 A NZ 744434A NZ 74443413 A NZ74443413 A NZ 74443413A NZ 744434 A NZ744434 A NZ 744434A
- Authority
- NZ
- New Zealand
- Prior art keywords
- methods
- compositions
- proteins
- norleucine misincorporation
- present
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- 239000000203 mixture Substances 0.000 title abstract 3
- LRQKBLKVPFOOQJ-YFKPBYRVSA-N L-norleucine Chemical compound CCCC[C@H]([NH3+])C([O-])=O LRQKBLKVPFOOQJ-YFKPBYRVSA-N 0.000 title abstract 2
- 108090000623 proteins and genes Proteins 0.000 title abstract 2
- 102000004169 proteins and genes Human genes 0.000 title abstract 2
- 108700028369 Alleles Proteins 0.000 abstract 1
- 241000894006 Bacteria Species 0.000 abstract 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 abstract 1
- 101100023016 Methanothermobacter marburgensis (strain ATCC BAA-927 / DSM 2133 / JCM 14651 / NBRC 100331 / OCM 82 / Marburg) mat gene Proteins 0.000 abstract 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 abstract 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 abstract 1
- 238000010348 incorporation Methods 0.000 abstract 1
- 101150095438 metK gene Proteins 0.000 abstract 1
- 229930182817 methionine Natural products 0.000 abstract 1
- 244000005700 microbiome Species 0.000 abstract 1
- 230000035772 mutation Effects 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 238000012261 overproduction Methods 0.000 abstract 1
- 230000014616 translation Effects 0.000 abstract 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
Abstract
The present invention relates to methods and compositions for preventing incorporation of norleucine into proteins during recombinant protein production in bacteria via mutations in metA and metK alleles that cause overproduction of methionine. The present invention also provides microorganism host cells and nucleic acid molecules for use with the methods and compositions provided herein.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261703142P | 2012-09-19 | 2012-09-19 | |
| US201361777700P | 2013-03-12 | 2013-03-12 | |
| NZ70590613 | 2013-09-19 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| NZ744434A true NZ744434A (en) | 2020-06-26 |
Family
ID=70612563
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NZ742412A NZ742412A (en) | 2012-09-19 | 2013-09-19 | Methods and compositions for preventing norleucine misincorporation into proteins |
| NZ744434A NZ744434A (en) | 2012-09-19 | 2013-09-19 | Methods and compositions for preventing norleucine misincorporation into proteins |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NZ742412A NZ742412A (en) | 2012-09-19 | 2013-09-19 | Methods and compositions for preventing norleucine misincorporation into proteins |
Country Status (1)
| Country | Link |
|---|---|
| NZ (2) | NZ742412A (en) |
-
2013
- 2013-09-19 NZ NZ742412A patent/NZ742412A/en not_active IP Right Cessation
- 2013-09-19 NZ NZ744434A patent/NZ744434A/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| NZ742412A (en) | 2020-04-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| PH12019500694A1 (en) | Factor viii compositions and methods of making and using the same | |
| MX2013003681A (en) | Engineered nucleic acids and methods of use thereof. | |
| MY180183A (en) | Methods and compositions for preventing norleucine misincorporation into proteins | |
| BR112015004467A2 (en) | method for controlling protein heterogeneity | |
| MX2016004564A (en) | Metabolically optimized cell culture. | |
| AU2017248555B2 (en) | Closed nucleic acid structures | |
| MX2018001040A (en) | Artificial nucleic acid molecules. | |
| WO2012135805A3 (en) | Delivery and formulation of engineered nucleic acids | |
| SG10201906673WA (en) | Artificial nucleic acid molecules | |
| EP4241784A3 (en) | Artificial nucleic acid molecules for improved protein expression | |
| BR112016023304A2 (en) | recombinant host cell engineered to overexpress helper proteins | |
| MX357803B (en) | Artificial nucleic acid molecules. | |
| SG11201403450VA (en) | Artificial nucleic acid molecules for improved protein or peptide expression | |
| BR112015019879A2 (en) | SPECIES COMPOSITIONS OF MODULATED LYSINE VARIANTS AND METHODS FOR THE PRODUCTION AND USE OF THEM | |
| BR112014018630A2 (en) | bleaching of chondroitin sulfate glycans | |
| MY173953A (en) | Recombinant host cells for the production of malonate | |
| IN2014DN02043A (en) | ||
| WO2016022377A3 (en) | Methods for modulating the glycosylation profile of recombinant proteins using dissolved oxygen | |
| BR112016023769A2 (en) | recombinant host cell for expression of proteins of interest | |
| SG11201601262QA (en) | CELL CULTURE MEDIUM AND PROCESS FOR CONTROLLING α-AMIDATION AND/OR C-TERMINAL AMINO ACID CLEAVAGE OF POLYPEPTIDES | |
| MX2016008258A (en) | Novel eukaryotic cells and methods for recombinantly expressing a product of interest. | |
| MX2017001432A (en) | Apoe mimetic peptides and higher potency to clear plasma cholesterol. | |
| PH12014501200A1 (en) | Bacteriophage gene 3 protein compositions and use as amyloid binding agents | |
| FI20115328A (en) | New cutinases, their production and uses | |
| MX2013001836A (en) | Recombinant fc-fusion protein of the fifth fibronectin type iii domain of dcc. |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PSEA | Patent sealed | ||
| RENW | Renewal (renewal fees accepted) |
Free format text: PATENT RENEWED FOR 1 YEAR UNTIL 19 SEP 2022 BY THOMSON REUTERS Effective date: 20210803 |
|
| RENW | Renewal (renewal fees accepted) |
Free format text: PATENT RENEWED FOR 1 YEAR UNTIL 19 SEP 2023 BY THOMSON REUTERS Effective date: 20220801 |
|
| LAPS | Patent lapsed |