NZ742412A - Methods and compositions for preventing norleucine misincorporation into proteins - Google Patents
Methods and compositions for preventing norleucine misincorporation into proteinsInfo
- Publication number
- NZ742412A NZ742412A NZ742412A NZ74241213A NZ742412A NZ 742412 A NZ742412 A NZ 742412A NZ 742412 A NZ742412 A NZ 742412A NZ 74241213 A NZ74241213 A NZ 74241213A NZ 742412 A NZ742412 A NZ 742412A
- Authority
- NZ
- New Zealand
- Prior art keywords
- methods
- compositions
- proteins
- norleucine misincorporation
- present
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- 239000000203 mixture Substances 0.000 title abstract 3
- LRQKBLKVPFOOQJ-YFKPBYRVSA-N L-norleucine Chemical compound CCCC[C@H]([NH3+])C([O-])=O LRQKBLKVPFOOQJ-YFKPBYRVSA-N 0.000 title abstract 2
- 102000004169 proteins and genes Human genes 0.000 title abstract 2
- 108090000623 proteins and genes Proteins 0.000 title abstract 2
- 108700028369 Alleles Proteins 0.000 abstract 2
- 241000894006 Bacteria Species 0.000 abstract 1
- 101100023016 Methanothermobacter marburgensis (strain ATCC BAA-927 / DSM 2133 / JCM 14651 / NBRC 100331 / OCM 82 / Marburg) mat gene Proteins 0.000 abstract 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 abstract 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 abstract 1
- 238000010348 incorporation Methods 0.000 abstract 1
- 101150060102 metA gene Proteins 0.000 abstract 1
- 101150086633 metAA gene Proteins 0.000 abstract 1
- 101150091110 metAS gene Proteins 0.000 abstract 1
- 101150095438 metK gene Proteins 0.000 abstract 1
- 101150043924 metXA gene Proteins 0.000 abstract 1
- 244000005700 microbiome Species 0.000 abstract 1
- 230000035772 mutation Effects 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 102200132944 rs74315449 Human genes 0.000 abstract 1
- 238000006467 substitution reaction Methods 0.000 abstract 1
- 230000014616 translation Effects 0.000 abstract 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
Abstract
The present invention relates to methods and compositions for preventing incorporation of norleucine into proteins during recombinant protein production in bacteria. The present invention also provides microorganism host cells and nucleic acid molecules encoding mutant metA alleles having R27C or Y294C substitutions and mutant metK alleles having V185E or c1132del mutations for use with the methods and compositions provided herein.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261703142P | 2012-09-19 | 2012-09-19 | |
| US201361777700P | 2013-03-12 | 2013-03-12 | |
| NZ70590613 | 2013-09-19 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| NZ742412A true NZ742412A (en) | 2020-04-24 |
Family
ID=70612563
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NZ742412A NZ742412A (en) | 2012-09-19 | 2013-09-19 | Methods and compositions for preventing norleucine misincorporation into proteins |
| NZ744434A NZ744434A (en) | 2012-09-19 | 2013-09-19 | Methods and compositions for preventing norleucine misincorporation into proteins |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NZ744434A NZ744434A (en) | 2012-09-19 | 2013-09-19 | Methods and compositions for preventing norleucine misincorporation into proteins |
Country Status (1)
| Country | Link |
|---|---|
| NZ (2) | NZ742412A (en) |
-
2013
- 2013-09-19 NZ NZ742412A patent/NZ742412A/en not_active IP Right Cessation
- 2013-09-19 NZ NZ744434A patent/NZ744434A/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| NZ744434A (en) | 2020-06-26 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PSEA | Patent sealed | ||
| RENW | Renewal (renewal fees accepted) |
Free format text: PATENT RENEWED FOR 1 YEAR UNTIL 19 SEP 2021 BY THOMSON REUTERS Effective date: 20200820 |
|
| RENW | Renewal (renewal fees accepted) |
Free format text: PATENT RENEWED FOR 1 YEAR UNTIL 19 SEP 2022 BY THOMSON REUTERS Effective date: 20210803 |
|
| RENW | Renewal (renewal fees accepted) |
Free format text: PATENT RENEWED FOR 1 YEAR UNTIL 19 SEP 2023 BY THOMSON REUTERS Effective date: 20220801 |
|
| LAPS | Patent lapsed |