NZ583974A - Methods for dynamic vector assembly of DNA cloning vector plasmids - Google Patents

Methods for dynamic vector assembly of DNA cloning vector plasmids

Info

Publication number
NZ583974A
NZ583974A NZ583974A NZ58397405A NZ583974A NZ 583974 A NZ583974 A NZ 583974A NZ 583974 A NZ583974 A NZ 583974A NZ 58397405 A NZ58397405 A NZ 58397405A NZ 583974 A NZ583974 A NZ 583974A
Authority
NZ
New Zealand
Prior art keywords
insert
vector
polynucleotide
docking point
docking
Prior art date
Application number
NZ583974A
Other languages
English (en)
Inventor
Thomas D Reed
Original Assignee
Intrexon Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Intrexon Corp filed Critical Intrexon Corp
Publication of NZ583974A publication Critical patent/NZ583974A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
NZ583974A 2004-05-18 2005-05-18 Methods for dynamic vector assembly of DNA cloning vector plasmids NZ583974A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US57201104P 2004-05-18 2004-05-18
NZ551447A NZ551447A (en) 2004-05-18 2005-05-18 Methods for dynamic vector assembly of DNA cloning vector plasmids

Publications (1)

Publication Number Publication Date
NZ583974A true NZ583974A (en) 2011-11-25

Family

ID=35450900

Family Applications (2)

Application Number Title Priority Date Filing Date
NZ551447A NZ551447A (en) 2004-05-18 2005-05-18 Methods for dynamic vector assembly of DNA cloning vector plasmids
NZ583974A NZ583974A (en) 2004-05-18 2005-05-18 Methods for dynamic vector assembly of DNA cloning vector plasmids

Family Applications Before (1)

Application Number Title Priority Date Filing Date
NZ551447A NZ551447A (en) 2004-05-18 2005-05-18 Methods for dynamic vector assembly of DNA cloning vector plasmids

Country Status (9)

Country Link
US (4) US20090170727A1 (de)
EP (2) EP1751298A4 (de)
JP (2) JP2007537755A (de)
CN (1) CN1981047B (de)
AU (1) AU2005248371B2 (de)
CA (1) CA2567337C (de)
ES (1) ES2603062T3 (de)
NZ (2) NZ551447A (de)
WO (1) WO2005116231A1 (de)

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US20080050808A1 (en) * 2002-10-09 2008-02-28 Reed Thomas D DNA modular cloning vector plasmids and methods for their use
EP1751298A4 (de) * 2004-05-18 2009-11-11 Intrexon Corp Verfahren zum dynamischen vektorzusammenbau von dna-klonierungsvektorplasmiden
US8153598B2 (en) * 2005-10-19 2012-04-10 Intrexon Corporation PKD ligands and polynucleotides encoding PKD ligands
US7943732B2 (en) 2006-06-05 2011-05-17 Intrexon Corporation AKT ligands and polynucleotides encoding AKT ligands
RU2468820C2 (ru) 2006-07-26 2012-12-10 Интрексон Корпорейшн Способы и композиции для лечения заболевания
US8993263B2 (en) 2006-08-07 2015-03-31 Intrexon Corporation PKA ligands and polynucleotides encoding PKA ligands
US8999666B2 (en) 2006-08-09 2015-04-07 Intrexon Corporation PKC ligands and polynucleotides encoding PKC ligands
US7897394B2 (en) 2006-09-21 2011-03-01 Intrexon Corporation Endoplasmic reticulum localization signals
US8283445B2 (en) 2006-11-13 2012-10-09 Intrexon Corporation Extracellular-signal-regulated-kinase (ERK) heteropolyligand polypeptide
US8729225B2 (en) 2006-11-13 2014-05-20 Intrexon Corporation Glycogen synthase kinase heteropolyligand polypeptide
US20090181458A1 (en) 2006-12-04 2009-07-16 Thomas David Reed Tubulo-vesicular structure localization signals
US7705122B2 (en) 2006-12-04 2010-04-27 Intrexon Corporation mTOR ligands and polynucleotides encoding mTOR ligands
JP5611814B2 (ja) 2007-03-27 2014-10-22 イントレキソン コーポレーション Ｍｅｋリガンドおよびｍｅｋリガンドをコードするポリヌクレオチド
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MX2010001993A (es) 2007-08-23 2010-03-11 Intrexon Corp Metodos y composiciones para diagnosticar una enfermedad.
DK3156414T3 (da) 2007-09-26 2020-03-09 Intrexon Corp Syntetisk 5'utrs ekspressionsvektorer og fremgangsmåder til øgning af transgen ekspression
EP2205249B1 (de) 2007-09-28 2018-11-07 Intrexon Corporation Therapeutische genschalterkonstrukte und bioreaktoren zur expression biotherapeutischer moleküle und ihre verwendung
RU2484132C2 (ru) 2007-10-08 2013-06-10 Интрексон Корпорейшн Модифицированные дендритные клетки и их применение в лечении злокачественных опухолей
JP2011509093A (ja) 2008-01-09 2011-03-24 イントレキソン コーポレーション Ｐａｉ−１機能の治療的阻害因子およびその使用法
CN103038343A (zh) 2010-03-23 2013-04-10 英特瑞克斯顿股份有限公司 条件表达治疗蛋白的载体，包含所述载体的宿主细胞，及其应用
WO2012122025A2 (en) 2011-03-04 2012-09-13 Intrexon Corporation Vectors conditionally expressing protein
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FR2986536A1 (fr) * 2012-02-08 2013-08-09 Lfb Biotechnologies Unites de transcription et leur utilisation dans des vecteurs d'expression (cho)
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WO2015066695A1 (en) * 2013-11-04 2015-05-07 Exact Sciences Corporation Multiple-control calibrators for dna quantitation
AU2017359342B2 (en) 2016-11-09 2022-02-17 Intrexon Corporation Frataxin expression constructs

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US8153598B2 (en) * 2005-10-19 2012-04-10 Intrexon Corporation PKD ligands and polynucleotides encoding PKD ligands

Also Published As

Publication number Publication date
NZ551447A (en) 2010-08-27
CN1981047B (zh) 2013-08-21
EP2484772A1 (de) 2012-08-08
JP5638491B2 (ja) 2014-12-10
AU2005248371A1 (en) 2005-12-08
JP2007537755A (ja) 2007-12-27
US20180023089A1 (en) 2018-01-25
AU2005248371B2 (en) 2011-12-15
WO2005116231A1 (en) 2005-12-08
CA2567337A1 (en) 2005-12-08
EP1751298A4 (de) 2009-11-11
CN1981047A (zh) 2007-06-13
US10036026B2 (en) 2018-07-31
US20090226976A1 (en) 2009-09-10
US9115361B2 (en) 2015-08-25
US20090170727A1 (en) 2009-07-02
EP1751298A1 (de) 2007-02-14
US20150057192A1 (en) 2015-02-26
JP2012024099A (ja) 2012-02-09
CA2567337C (en) 2017-12-12
ES2603062T3 (es) 2017-02-23
EP2484772B1 (de) 2016-08-17

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